Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,666,978 A→G 5.3% L290S (TTG→TCG) ‡ hcaT ← putative 3‑phenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,666,9780AG 5.3% 309.8 / 3.9 94L290S (TTG→TCG) ‡hcaTputative 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base A (48/41);  new base G (2/3);  total (50/44)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA  >  NC_000913/2666832‑2667126
                                                                                                                                                  |                                                                                                                                                    
tACTGCCGCTCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAAc                                                                                                                                                    <  3:911278/149‑1 (MQ=255)
tACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACg                                                                                                                                                   >  1:869946/1‑149 (MQ=255)
     ccgccCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGt                                                                                                                                               >  1:639143/1‑149 (MQ=255)
      cgccCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTa                                                                                                                                              <  1:679567/149‑1 (MQ=255)
      cgccCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTa                                                                                                                                              <  3:752836/149‑1 (MQ=255)
        cccATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGtt                                                                                                                                           <  2:869946/149‑1 (MQ=255)
         ccATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGtt                                                                                                                                           <  1:1048850/149‑1 (MQ=255)
         ccATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGtt                                                                                                                                           >  4:847209/1‑149 (MQ=255)
              gcgACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTcc                                                                                                                                      >  1:117393/1‑149 (MQ=255)
                gACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACTACGGCGTTGCCGTGGTTgatagtg                                                                                                                                    <  1:474220/149‑7 (MQ=255)
                    gCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAAt                                                                                                                                <  3:974323/149‑1 (MQ=255)
                      agagTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGc                                                                                                                              <  4:621320/149‑1 (MQ=255)
                      agagTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGc                                                                                                                              >  2:679494/1‑149 (MQ=255)
                      agagTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGc                                                                                                                              >  3:856121/1‑149 (MQ=255)
                        agTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGccc                                                                                                                            >  2:1067149/1‑149 (MQ=255)
                          tAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCa                                                                                                                          >  1:886275/1‑149 (MQ=255)
                            aaCCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAgc                                                                                                                        <  2:917031/149‑1 (MQ=255)
                                gCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCAc                                                                                                                    <  2:786348/149‑1 (MQ=255)
                                 ccTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACt                                                                                                                   >  1:588383/1‑149 (MQ=255)
                                    gTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTAcg                                                                                                                >  4:383337/1‑149 (MQ=255)
                                     tAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTAcgc                                                                                                               <  2:208101/149‑1 (MQ=255)
                                      aaaCGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTAcgcc                                                                                                              <  1:864214/149‑1 (MQ=255)
                                      aaaCGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTAcgcc                                                                                                              <  4:688461/149‑1 (MQ=255)
                                      aaaCGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTAcgcc                                                                                                              >  1:41219/1‑149 (MQ=255)
                                       aaCGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTc                                                                                                                                       >  4:525935/1‑123 (MQ=255)
                                       aaCGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTc                                                                                                                                       <  3:525935/123‑1 (MQ=255)
                                              gACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCg                                                                                                      >  2:638758/1‑149 (MQ=255)
                                               aCTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGc                                                                                                     >  3:207498/1‑149 (MQ=255)
                                                cTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGcc                                                                                                    >  2:415923/1‑149 (MQ=255)
                                                cTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGcc                                                                                                    <  3:383337/149‑1 (MQ=255)
                                                cTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGcc                                                                                                    <  1:166047/149‑1 (MQ=255)
                                                 ttCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCg                                                                                                   >  3:82892/1‑149 (MQ=255)
                                                 ttCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCg                                                                                                   <  2:397135/149‑1 (MQ=255)
                                                 ttCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCg                                                                                                   >  2:530242/1‑149 (MQ=255)
                                                 ttCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCg                                                                                                   >  4:1058462/1‑149 (MQ=255)
                                                        cccTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCaa                                                                                            >  3:214771/1‑149 (MQ=255)
                                                         ccTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAAc                                                                                           <  2:773113/149‑1 (MQ=255)
                                                          cTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACa                                                                                          <  2:462988/149‑1 (MQ=255)
                                                          cTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACa                                                                                          <  2:117393/149‑1 (MQ=255)
                                                           tGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAg                                                                                         >  2:138145/1‑149 (MQ=255)
                                                               gAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAAGAGCCGCAATGGAGAATTTGCACCACTATCAACCACGGCACCGCCGTAGTTGCTCCCATAATGCGCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCata                                                                                     >  2:27415/1‑149 (MQ=255)
                                                                     caATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGt                                                                               >  1:668884/1‑149 (MQ=255)
                                                                       atatAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGc                                                                             >  4:943182/1‑149 (MQ=255)
                                                                           aaCGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACTACGGCGTTGCCGTGGTTga                                                                                                                                         >  2:247798/1‑84 (MQ=38)
                                                                           aaCGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACTACGGCGTTGCCGTGGTTga                                                                                                                                         <  1:247798/85‑2 (MQ=38)
                                                                           aaCGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACTACGGCGTTGCCGTGGTTga                                                                                                                                         <  3:503589/85‑2 (MQ=38)
                                                                           aaCGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACTACGGCGTTGCCGTGGTTga                                                                                                                                         >  4:503589/1‑84 (MQ=38)
                                                                              gCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACaa                                                                      <  1:638758/149‑1 (MQ=255)
                                                                              gCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACaa                                                                      >  3:618894/1‑149 (MQ=255)
                                                                               cATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAc                                                                     <  1:311760/149‑1 (MQ=255)
                                                                                  ggcggcCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcggc                                                                  <  4:571337/149‑1 (MQ=255)
                                                                                  ggcggcCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcggc                                                                  <  1:415923/149‑1 (MQ=255)
                                                                                   gcggcCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcggcg                                                                 <  3:943182/149‑1 (MQ=255)
                                                                                        cAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGaaaa                                                            <  2:1007491/149‑1 (MQ=255)
                                                                                         aGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAg                                                           >  4:996122/1‑149 (MQ=255)
                                                                                         aGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAg                                                           >  4:114134/1‑149 (MQ=255)
                                                                                          ggTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGt                                                          <  4:445678/149‑1 (MQ=255)
                                                                                          ggTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGt                                                          >  3:339359/1‑149 (MQ=255)
                                                                                             ggCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTtta                                                       <  1:803952/149‑1 (MQ=255)
                                                                                                aGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttatta                                                    <  3:996122/149‑1 (MQ=255)
                                                                                                aGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttatta                                                    >  3:456390/1‑149 (MQ=255)
                                                                                                aGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttatta                                                    <  3:114134/149‑1 (MQ=255)
                                                                                                aGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAACATATCGCGTGCACTACAACGGCGGAAAAGTttatta                                                    <  1:641148/149‑1 (MQ=255)
                                                                                                          ggTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCaa                                          >  1:535932/1‑149 (MQ=255)
                                                                                                           gTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCaaa                                         <  3:341698/149‑1 (MQ=255)
                                                                                                           gTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCaaa                                         <  3:1058462/149‑1 (MQ=255)
                                                                                                                gCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATaa                                    <  2:846425/149‑1 (MQ=255)
                                                                                                                gCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATaa                                    <  4:613250/149‑1 (MQ=255)
                                                                                                                 cAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAAt                                   <  1:1067149/149‑1 (MQ=255)
                                                                                                                   aTGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCaa                                                                                            <  1:979061/90‑1 (MQ=255)
                                                                                                                   aTGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCaa                                                                                            >  2:979061/1‑90 (MQ=255)
                                                                                                                    tGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTAATTACTCAGCGCAAAGATAATGAc                                >  2:719420/1‑149 (MQ=255)
                                                                                                                        gAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTcc                            >  3:310543/1‑149 (MQ=255)
                                                                                                                             tGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGccac                       <  2:535932/149‑1 (MQ=255)
                                                                                                                               caccacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGccacca                     >  2:94813/1‑149 (MQ=255)
                                                                                                                                accacTATGAACTACGGCAACGCCGTAGTTGCTCCCCTAATGCCCCAGCGCCCTAGGCCGCACATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGATTATTACTCAGCGCAAAAATAACGACTTCCGCcaccac                    >  4:949105/1‑149 (MQ=255)
                                                                                                                                accacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCcaccac                    >  3:58734/1‑149 (MQ=255)
                                                                                                                                accacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCcaccac                    >  2:827118/1‑149 (MQ=255)
                                                                                                                                accacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCcaccac                    >  1:71956/1‑149 (MQ=255)
                                                                                                                                  cacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGc                  >  4:243025/1‑149 (MQ=255)
                                                                                                                                     tATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCa               >  2:987581/1‑149 (MQ=255)
                                                                                                                                     tATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCa               >  4:734847/1‑149 (MQ=255)
                                                                                                                                      aTCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAg              >  3:213110/1‑149 (MQ=255)
                                                                                                                                         aaCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGa           <  4:213110/149‑1 (MQ=255)
                                                                                                                                         aaCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGa           >  3:209872/1‑149 (MQ=255)
                                                                                                                                          aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGAc          >  1:516775/1‑149 (MQ=255)
                                                                                                                                          aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGAc          >  3:911140/1‑149 (MQ=255)
                                                                                                                                          aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGAc          >  4:971207/1‑149 (MQ=255)
                                                                                                                                          aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGAc          <  3:107918/149‑1 (MQ=255)
                                                                                                                                          aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGAc          >  3:819045/1‑149 (MQ=255)
                                                                                                                                            cACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACca        >  1:904245/1‑149 (MQ=255)
                                                                                                                                                 cAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAAt   <  4:791755/149‑1 (MQ=255)
                                                                                                                                                 cAACGCCGTAGTTGCTCCCATATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGaaaa                                                            <  2:164231/91‑1 (MQ=255)
                                                                                                                                                 cAACGCCGTAGTTGCTCCCATATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGaaaa                                                            >  1:164231/1‑91 (MQ=255)
                                                                                                                                                  aaCGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATa  <  4:819045/149‑1 (MQ=255)
                                                                                                                                                  aaCGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATa  >  4:488321/1‑149 (MQ=255)
                                                                                                                                                  aaCGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATa  >  4:275423/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATA  >  NC_000913/2666832‑2667126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: