Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,700,863 G→C 6.5% P46A (CCG→GCG) ‡ acpS ← holo‑[acyl‑carrier‑protein] synthase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,700,8630GC 6.5% 316.1 / 10.9 93P46A (CCG→GCG) ‡acpSholo‑[acyl‑carrier‑protein] synthase 1
Reads supporting (aligned to +/- strand):  ref base G (43/44);  new base C (3/3);  total (46/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

CCTCGCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGC  >  NC_000913/2700716‑2701002
                                                                                                                                                   |                                                                                                                                           
ccTCGCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGc                                                                                                                                            <  3:292898/149‑1 (MQ=255)
 cTCGCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCt                                                                                                                                           >  2:565665/1‑149 (MQ=255)
  tCGCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCtg                                                                                                                                          <  4:258089/149‑1 (MQ=255)
    gCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCtggt                                                                                                                                        <  3:442481/149‑1 (MQ=255)
    gCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCtggt                                                                                                                                        <  1:1016242/149‑1 (MQ=255)
        cATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGgtggtg                                                                                                                                    >  1:176450/1‑149 (MQ=255)
         aTAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGc                                                                                                                                   <  2:229370/149‑1 (MQ=255)
            gccgtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGtt                                                                                                                                <  2:330097/149‑1 (MQ=255)
            gccgtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGtt                                                                                                                                <  4:711452/149‑1 (MQ=255)
            gccgtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCACCAGCCGGTGCGtt                                                                                                                                <  3:256330/149‑1 (MQ=255)
               gtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTc                                                                                                                             <  1:782144/149‑1 (MQ=255)
               gtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCACCAGCCGGTGCGTTTTc                                                                                                                             <  2:14160/149‑1 (MQ=255)
                 agccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCa                                                                                                                           <  1:934989/149‑1 (MQ=255)
                     gtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATa                                                                                                                       <  4:1003853/149‑1 (MQ=255)
                      tGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAg                                                                                                                      <  1:244972/149‑1 (MQ=255)
                        gTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGcc                                                                                                                    >  4:1066379/1‑149 (MQ=255)
                          ttGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCa                                                                                                                  <  2:481021/149‑1 (MQ=255)
                                     atcatTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCTAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGc                                                                                                       <  1:190982/149‑1 (MQ=255)
                                     atcatTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGc                                                                                                       <  2:588194/149‑1 (MQ=255)
                                           gAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATa                                                                                                 >  1:385995/1‑149 (MQ=255)
                                                 ttCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcggc                                                                                           >  1:557210/1‑149 (MQ=255)
                                                 ttCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcggc                                                                                           >  1:560743/1‑149 (MQ=255)
                                                 ttCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcggc                                                                                           >  1:1048963/1‑149 (MQ=255)
                                                 ttCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcggc                                                                                           >  2:841206/1‑149 (MQ=255)
                                                 ttCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcggc                                                                                           >  1:271634/1‑149 (MQ=255)
                                                 ttCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcggc                                                                                           >  1:124905/1‑149 (MQ=255)
                                                 ttCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcggc                                                                                           >  1:896499/1‑149 (MQ=255)
                                                    aaaTTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTg                                                                                        >  4:145177/1‑149 (MQ=255)
                                                      aTTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGcc                                                                                      >  2:411289/1‑149 (MQ=255)
                                                      aTTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGcc                                                                                      >  1:990797/1‑149 (MQ=255)
                                                      aTTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGcc                                                                                      >  1:491148/1‑149 (MQ=255)
                                                           ttAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCACCAGCCGGTGCGTTTTctg                                                                                                                           >  3:427838/1‑105 (MQ=38)
                                                                cGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCAc                                                                            <  4:261082/149‑1 (MQ=255)
                                                                cGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCAc                                                                            >  3:532814/1‑149 (MQ=255)
                                                                 gCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCACCAGCCGGTGCGttt                                                                                                                               <  2:837002/97‑1 (MQ=38)
                                                                 gCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCACCAGCCGGTGCGttt                                                                                                                               >  1:837002/1‑97 (MQ=38)
                                                                  ccAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCg                                                                          <  3:961623/149‑1 (MQ=255)
                                                                   cAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCgg                                                                         >  3:330248/1‑149 (MQ=255)
                                                                   cAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCgg                                                                         <  2:51608/149‑1 (MQ=255)
                                                                   cAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTAACGCTTAATACGCGGCGTGCCAGGCGATCACCgg                                                                         >  2:14236/1‑149 (MQ=255)
                                                                     gACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGAt                                                                       >  4:4336/1‑149 (MQ=255)
                                                                      aCCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATc                                                                      <  2:704062/149‑1 (MQ=255)
                                                                       ccATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCg                                                                     >  3:530903/1‑149 (MQ=255)
                                                                          ttGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGc                                                                  >  1:765439/1‑149 (MQ=255)
                                                                          ttGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGc                                                                  >  1:1061553/1‑149 (MQ=255)
                                                                          ttGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGc                                                                  >  3:307311/1‑149 (MQ=255)
                                                                            gCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGa                                                                <  2:557210/149‑1 (MQ=255)
                                                                              ggATCGCGGTGCCAAACGCTTTTGCTGCGGCTTCTGTCACAGCAAAACGCTTCGCCGGAAATCGCACCGGCTGGTGGTGCGTTTTGCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTTCCAGACGATCACCGGATCGGGCGAtt                                                              <  4:163906/149‑2 (MQ=255)
                                                                               gATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCa                                                             <  1:841206/149‑1 (MQ=255)
                                                                               gATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCa                                                             >  2:822160/1‑149 (MQ=255)
                                                                                 tCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCAcc                                                           >  1:756455/1‑149 (MQ=255)
                                                                                        gCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGa                                                    <  1:945036/149‑1 (MQ=255)
                                                                                         ccAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGAt                                                   <  1:662460/149‑1 (MQ=255)
                                                                                          cAAACGCTTTTGCTGCGGCTTCTTTCACAGCATAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCg                                                                                                              <  3:668883/89‑1 (MQ=255)
                                                                                          cAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCg                                                                                                              >  4:668883/1‑89 (MQ=255)
                                                                                          cAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATg                                                  >  1:719907/1‑149 (MQ=255)
                                                                                           aaaCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgc                                                 >  1:156316/1‑149 (MQ=255)
                                                                                           aaaCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgc                                                 >  2:919471/1‑149 (MQ=255)
                                                                                           aaaCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgc                                                 >  3:261499/1‑149 (MQ=255)
                                                                                           aaaCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgc                                                 <  2:728935/149‑1 (MQ=255)
                                                                                            aaCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcg                                                >  4:397465/1‑149 (MQ=255)
                                                                                             aCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGAt                                                   <  2:984826/145‑1 (MQ=255)
                                                                                             aCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGAt                                                   >  1:984826/1‑145 (MQ=255)
                                                                                               gCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCGGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcgagc                                             >  3:188789/1‑149 (MQ=255)
                                                                                                 ttttGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGAt                                                                       <  4:38933/121‑1 (MQ=255)
                                                                                                 ttttGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGAt                                                                       >  3:38933/1‑121 (MQ=255)
                                                                                                        gcGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCcac                                    <  4:109830/149‑1 (MQ=255)
                                                                                                        gcGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCcac                                    <  4:931750/149‑1 (MQ=255)
                                                                                                        gcGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCcac                                    <  2:491148/149‑1 (MQ=255)
                                                                                                          ggCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACaa                                  <  4:344396/149‑1 (MQ=255)
                                                                                                                 ttCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGt                           <  2:990797/149‑1 (MQ=255)
                                                                                                                 ttCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGt                           <  1:822160/149‑1 (MQ=255)
                                                                                                                   cacaGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCTCATTCGTTAACGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCTCTTCGATGCGAGCGATCTTCACAATATCCGTGc                         <  4:705585/149‑1 (MQ=255)
                                                                                                                   cacaGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGc                         >  1:360089/1‑149 (MQ=255)
                                                                                                                   cacaGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGc                         >  4:334986/1‑149 (MQ=255)
                                                                                                                    acaGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGcc                        >  3:582472/1‑149 (MQ=255)
                                                                                                                    acaGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGcc                        >  4:45953/1‑149 (MQ=255)
                                                                                                                        cAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTaaa                    <  2:657303/149‑1 (MQ=255)
                                                                                                                             cGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTaa               <  1:411289/149‑1 (MQ=255)
                                                                                                                              gCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAat              <  1:919471/149‑1 (MQ=255)
                                                                                                                                     cAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCa       >  2:709071/1‑149 (MQ=255)
                                                                                                                                     cAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCa       >  1:1027549/1‑149 (MQ=255)
                                                                                                                                       gAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGAt                                          >  3:918097/1‑112 (MQ=255)
                                                                                                                                       gAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGAt                                          <  4:918097/112‑1 (MQ=255)
                                                                                                                                       gAAAACGCACCACCAGCCGGTGCGTTTTCCAGATAGCCCATCCGTTATCGCTTAACACGCGGCGTGTCAGGCGATCACCGGATCGGGCGATCACCGATCCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCAtt     >  2:547467/1‑149 (MQ=255)
                                                                                                                                        aaaaCGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTa    <  1:297176/149‑1 (MQ=255)
                                                                                                                                        aaaaCGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTa    >  4:837707/1‑149 (MQ=255)
                                                                                                                                         aaaCGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAg   <  3:837707/149‑1 (MQ=255)
                                                                                                                                         aaaCGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAg   <  3:190394/149‑1 (MQ=255)
                                                                                                                                         aaaCGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAg   <  2:551171/149‑1 (MQ=255)
                                                                                                                                          aaCGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGc                                                         <  4:1066712/94‑1 (MQ=255)
                                                                                                                                          aaCGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGc                                                         >  3:1066712/1‑94 (MQ=255)
                                                                                                                                          aaCGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGc  <  4:532814/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
CCTCGCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGC  >  NC_000913/2700716‑2701002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: