Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,775,085 T→C 5.9% intergenic (+64/‑460) yfjW → / → ypjI CP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,775,0850TC 5.9% 382.6 / 10.6 120intergenic (+64/‑460)yfjW/ypjICP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base T (56/56);  new base C (3/4);  total (59/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01

Only 100 of 126 total aligned reads displayed.

ATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAA  >  NC_000913/2774937‑2775233
                                                                                                                                                    |                                                                                                                                                    
atTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGt                                                                                                                                                      <  3:62931/149‑1 (MQ=255)
 tttGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGtt                                                                                                                                                     >  3:305064/1‑149 (MQ=255)
   tGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGtttt                                                                                                                                                   <  3:381505/149‑1 (MQ=255)
    ggTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGttttt                                                                                                                                                  <  2:267912/149‑1 (MQ=255)
       ttAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTaaa                                                                                                                                               <  4:884719/149‑1 (MQ=255)
       ttAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTaaa                                                                                                                                               <  4:464423/149‑1 (MQ=255)
       ttAGGTCTGCTAGCAACTTCTATAAATTTCATTCATTCTTACTTTAAGAATGATGTGAATAAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGTAGCCTTTTTATGACTATTTATTAACATATCCTAAGTTAAGCCCGATTTTaaa                                                                                                                                               <  2:1002034/149‑1 (MQ=255)
          ggTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCa                                                                                                                                            <  4:305064/149‑1 (MQ=255)
          ggTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCa                                                                                                                                            <  4:676225/149‑1 (MQ=255)
           gTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGCTTTAAAAACAg                                                                                                                                           <  1:328617/149‑1 (MQ=255)
                cTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTg                                                                                                                                      >  4:828851/1‑149 (MQ=255)
                  aGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGCTTTAAAAACAGGCttaac                                                                                                                                    <  3:687612/149‑4 (MQ=255)
                   gCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGc                                                                                                                                   <  2:627962/149‑1 (MQ=255)
                    cAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGTATTTGGCTTCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCGTGTTTTTTAAGCAGGCTTGAGCg                                                                                                                                  >  2:819851/1‑149 (MQ=255)
                      aTTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTg                                                                                                                                >  4:546747/1‑149 (MQ=255)
                           tataAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCg                                                                                                                           >  2:362566/1‑149 (MQ=255)
                                   tcattcattcTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTaaa                                                                                                                                               <  1:757614/121‑1 (MQ=255)
                                   tcattcattcTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTaaa                                                                                                                                               >  2:757614/1‑121 (MQ=255)
                                      ttcattcTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                                                                                >  4:1038975/1‑149 (MQ=255)
                                      ttcattcTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                                                                                >  1:634273/1‑149 (MQ=255)
                                      ttcattcTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                                                                                <  1:355496/149‑1 (MQ=255)
                                            cTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCtt                                                                                                          <  2:304434/149‑1 (MQ=255)
                                             ttACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCttt                                                                                                         >  2:676049/1‑149 (MQ=255)
                                                cTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTaaa                                                                                                      <  1:631333/149‑1 (MQ=255)
                                                cTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTaaa                                                                                                      <  4:604835/149‑1 (MQ=255)
                                                      gAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCAt                                                                                                                  >  1:655148/1‑131 (MQ=255)
                                                      gAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCAt                                                                                                                  <  2:655148/131‑1 (MQ=255)
                                                      gAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCg                                                                                                >  1:452002/1‑149 (MQ=255)
                                                           atgTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTTGCGttttt                                                                                           <  3:998188/149‑1 (MQ=255)
                                                           atgTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGttttt                                                                                           >  2:899191/1‑149 (MQ=255)
                                                           atgTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGttttt                                                                                           <  1:329495/149‑1 (MQ=255)
                                                            tgtgAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTg                                                                                          >  3:274682/1‑149 (MQ=255)
                                                            tgtgAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTg                                                                                          >  2:688727/1‑149 (MQ=255)
                                                            tgtgAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTCTTg                                                                                          >  4:780112/1‑149 (MQ=255)
                                                             gtgAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGt                                                                                         <  3:1038975/149‑1 (MQ=255)
                                                               gaatgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAACAGGCTTGAGCGTGAAGCGGACAGTCATACCTGCTTTAAAGTGGCGTTTTTGTCg                                                                                       >  1:316639/1‑149 (MQ=255)
                                                                aatgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGc                                                                                                 <  2:265661/138‑1 (MQ=255)
                                                                aatgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGt                                                                                      >  3:978967/1‑149 (MQ=255)
                                                                 atgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGtt                                                                                     >  2:798183/1‑149 (MQ=255)
                                                                  tgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTa                                                                                    <  4:873500/149‑1 (MQ=255)
                                                                   gaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAc                                                                                   <  2:634273/149‑1 (MQ=255)
                                                                   gaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAc                                                                                   >  1:42806/1‑149 (MQ=255)
                                                                     aaaTTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAccc                                                                                 <  3:546747/149‑1 (MQ=255)
                                                                     aaaTTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAccc                                                                                 <  3:71232/149‑1 (MQ=255)
                                                                        tttATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATa                                                                              <  1:912444/149‑1 (MQ=255)
                                                                           aTTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCt                                                                           >  2:993153/1‑149 (MQ=255)
                                                                               ttGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTatat                                                                       >  2:542088/1‑149 (MQ=255)
                                                                                  tAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATAtttt                                                                    >  3:961664/1‑149 (MQ=255)
                                                                                   aaCGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCt                                                                                                           <  1:1081050/109‑1 (MQ=255)
                                                                                   aaCGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCt                                                                                                           >  2:1081050/1‑109 (MQ=255)
                                                                                   aaCGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTc                                                                   <  2:81984/149‑1 (MQ=255)
                                                                                    aCGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCt                                                                  <  4:379881/149‑1 (MQ=255)
                                                                                        aTTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCtgttg                                                              >  3:872185/1‑149 (MQ=255)
                                                                                          ttGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGGCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTgttgtt                                                            <  3:933102/149‑1 (MQ=255)
                                                                                          aaggCTCGAATTGGAGCCTTTTTATGACTATTCCTTAAAAAATCCCAAGTTAAGACTGTTTTTAAAGCAGGTTTGAGAGTGAATCGGACACTAATACCTGCTTTAAAGTTGCGTTTTTGTCGTTACCCATACCTATACTTTCTtttatt                                                            >  4:760314/3‑149 (MQ=255)
                                                                                               tCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGacac                                                                                                                      <  3:257743/86‑1 (MQ=255)
                                                                                               tCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGacac                                                                                                                      >  4:257743/1‑86 (MQ=255)
                                                                                               tCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACt                                                       >  4:211554/1‑149 (MQ=255)
                                                                                               tCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACt                                                       >  1:112659/1‑149 (MQ=255)
                                                                                                 cAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                                                                                >  2:49750/1‑90 (MQ=255)
                                                                                                 cAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                                                                                <  1:49750/90‑1 (MQ=255)
                                                                                                 cAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGc                                                     <  3:970400/149‑1 (MQ=255)
                                                                                                  aaTTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGcc                                                    >  4:319815/1‑149 (MQ=255)
                                                                                                   aTTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCa                                                   >  2:867882/1‑149 (MQ=255)
                                                                                                    ttGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAg                                                  >  1:493934/1‑149 (MQ=255)
                                                                                                         gCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTg                                             <  4:411863/149‑1 (MQ=255)
                                                                                                          ccTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGa                                            <  4:401028/149‑1 (MQ=255)
                                                                                                           cTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAg                                           <  2:493934/149‑1 (MQ=255)
                                                                                                           cTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAg                                           >  4:862498/1‑149 (MQ=255)
                                                                                                            tttttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCATTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGc                                          >  4:611088/1‑149 (MQ=255)
                                                                                                             ttttATGACTATTTATTAAAATATCCTACGTCACGCCTCTGTAGAAAGCAGGCTTGAGCATGAAGCTGACACTCATACCTGCCTTAAAGTGGCGTTTTTGTCGTTTCCCCTACCTACATTTACTGTTTTTTAACGGCCAGAACTGAGCg                                         <  4:947413/149‑1 (MQ=255)
                                                                                                             ttttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCg                                         <  3:212577/149‑1 (MQ=255)
                                                                                                             ttttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCg                                         <  1:993153/149‑1 (MQ=255)
                                                                                                               ttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGt                                       <  4:577343/149‑1 (MQ=255)
                                                                                                                   gACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGaa                                   >  1:44392/1‑149 (MQ=255)
                                                                                                                    aCTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGaaa                                  <  3:862498/149‑1 (MQ=255)
                                                                                                                     cTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGaaaa                                 <  3:760314/149‑1 (MQ=255)
                                                                                                                       atttattAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGc                               <  1:717550/149‑1 (MQ=255)
                                                                                                                       atttattAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGc                               <  2:735939/149‑1 (MQ=255)
                                                                                                                             taAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGaaa                         >  2:689788/1‑149 (MQ=255)
                                                                                                                               aaaTATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAata                       <  2:452002/149‑1 (MQ=255)
                                                                                                                               aaaTATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAata                       >  4:925182/1‑149 (MQ=255)
                                                                                                                               aaaTATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAata                       <  4:69129/149‑1 (MQ=255)
                                                                                                                                aaTATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAACGCCAGAAatat                      <  3:611088/149‑1 (MQ=255)
                                                                                                                                  tatCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGCACTGAGCGGTTGAAAAGCCAGAAATATGc                    <  3:925100/149‑1 (MQ=255)
                                                                                                                                     ccTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGa                 >  1:1100910/1‑149 (MQ=255)
                                                                                                                                     ccTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGATACCCATACCTATATTTTCTGTAGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGa                 <  1:524860/149‑1 (MQ=255)
                                                                                                                                      cTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATAc                                                                             >  1:394015/1‑88 (MQ=255)
                                                                                                                                      cTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCtgt                                                                >  4:934121/1‑101 (MQ=255)
                                                                                                                                      cTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAGCTGAGCGGTTGAAAAGCCAGAAATATGCCGAg                >  1:205233/1‑149 (MQ=255)
                                                                                                                                      cTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAg                >  2:914552/1‑149 (MQ=255)
                                                                                                                                           tcaaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGTCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCa                             <  2:478327/129‑1 (MQ=255)
                                                                                                                                           tcaaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGa                                            >  3:197367/3‑116 (MQ=255)
                                                                                                                                           tcaaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGa                                            <  4:197367/114‑1 (MQ=255)
                                                                                                                                             aaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCgg         >  3:506503/1‑149 (MQ=255)
                                                                                                                                                 cTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATg     >  1:916077/1‑149 (MQ=255)
                                                                                                                                                  tGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATgg    >  1:741062/1‑149 (MQ=255)
                                                                                                                                                  tGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGACAGAAATATGCCGAGCCAGCGGCATgg    >  4:344288/1‑149 (MQ=255)
                                                                                                                                                    tttttAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGaa  >  3:744832/1‑149 (MQ=255)
                                                                                                                                                    tttttAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCACGGaa  <  1:5058/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAA  >  NC_000913/2774937‑2775233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: