Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,775,093 G→A 6.5% intergenic (+72/‑452) yfjW → / → ypjI CP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,775,0930GA 6.5% 379.3 / 11.7 123intergenic (+72/‑452)yfjW/ypjICP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (58/57);  new base A (4/4);  total (62/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

Only 100 of 123 total aligned reads displayed.

GGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCCAG  >  NC_000913/2774947‑2775240
                                                                                                                                                  |                                                                                                                                                   
ggTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCa                                                                                                                                                   <  4:305064/149‑1 (MQ=255)
ggTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCa                                                                                                                                                   <  4:676225/149‑1 (MQ=255)
 gTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAg                                                                                                                                                  <  4:532280/149‑1 (MQ=255)
 gTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGCTTTAAAAACAg                                                                                                                                                  <  1:328617/149‑1 (MQ=255)
     gCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCtt                                                                                                                                              <  4:1009282/149‑1 (MQ=255)
      cTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTg                                                                                                                                             >  4:828851/1‑149 (MQ=255)
         gCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGc                                                                                                                                          <  2:627962/149‑1 (MQ=255)
          cAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGTATTTGGCTTCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCGTGTTTTTTAAGCAGGCTTGAGCg                                                                                                                                         >  2:819851/1‑149 (MQ=255)
            aTTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTg                                                                                                                                       >  4:546747/1‑149 (MQ=255)
              ttCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGaa                                                                                                                                     <  1:731012/149‑1 (MQ=255)
                 tataAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCg                                                                                                                                  >  2:362566/1‑149 (MQ=255)
                            ttcattcTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                                                                                       <  1:355496/149‑1 (MQ=255)
                            ttcattcTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                                                                                       >  1:634273/1‑149 (MQ=255)
                              cattcTTAAAGTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTACTTATTAAAATATCCTAACTTAAGCCTGTTATTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCt                                                                                                                     >  4:345263/1‑149 (MQ=255)
                                  cTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCtt                                                                                                                 <  2:304434/149‑1 (MQ=255)
                                   ttACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCttt                                                                                                                >  2:676049/1‑149 (MQ=255)
                                            gAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCAt                                                                                                                         <  2:655148/131‑1 (MQ=255)
                                            gAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCAt                                                                                                                         >  1:655148/1‑131 (MQ=255)
                                            gAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCg                                                                                                       >  1:452002/1‑149 (MQ=255)
                                                 atgTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTTGCGttttt                                                                                                  <  3:998188/149‑1 (MQ=255)
                                                 atgTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGttttt                                                                                                  <  1:329495/149‑1 (MQ=255)
                                                 atgTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGttttt                                                                                                  >  2:899191/1‑149 (MQ=255)
                                                  tgtgAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTg                                                                                                 >  2:688727/1‑149 (MQ=255)
                                                  tgtgAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTg                                                                                                 >  3:274682/1‑149 (MQ=255)
                                                  tgtgAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTCTTg                                                                                                 >  4:780112/1‑149 (MQ=255)
                                                   gtgAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGt                                                                                                <  3:1038975/149‑1 (MQ=255)
                                                     gaatgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAACAGGCTTGAGCGTGAAGCGGACAGTCATACCTGCTTTAAAGTGGCGTTTTTGTCg                                                                                              >  1:316639/1‑149 (MQ=255)
                                                      aatgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGc                                                                                                        <  2:265661/138‑1 (MQ=255)
                                                      aatgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGt                                                                                             >  3:978967/1‑149 (MQ=255)
                                                       atgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGtt                                                                                            >  2:798183/1‑149 (MQ=255)
                                                         gaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAc                                                                                          <  2:634273/149‑1 (MQ=255)
                                                         gaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAc                                                                                          >  1:42806/1‑149 (MQ=255)
                                                           aaaTTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAccc                                                                                        <  3:71232/149‑1 (MQ=255)
                                                           aaaTTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAccc                                                                                        <  3:546747/149‑1 (MQ=255)
                                                              tttATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATa                                                                                     <  1:912444/149‑1 (MQ=255)
                                                                 aTTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCt                                                                                  >  2:993153/1‑149 (MQ=255)
                                                                     ttGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTatat                                                                              >  2:542088/1‑149 (MQ=255)
                                                                         aaCGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCt                                                                                                                  >  2:1081050/1‑109 (MQ=255)
                                                                         aaCGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCt                                                                                                                  <  1:1081050/109‑1 (MQ=255)
                                                                         aaCGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTc                                                                          <  2:81984/149‑1 (MQ=255)
                                                                          aCGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCt                                                                         <  4:379881/149‑1 (MQ=255)
                                                                            taaaaaggCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCtgt                                                                       >  1:990322/7‑149 (MQ=255)
                                                                              aTTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCtgttg                                                                     >  4:212577/1‑149 (MQ=255)
                                                                              aTTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCtgttg                                                                     >  3:872185/1‑149 (MQ=255)
                                                                                ttGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGGCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTgttgtt                                                                   <  3:933102/149‑1 (MQ=255)
                                                                                    cTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAAc                                                               <  3:801602/149‑1 (MQ=255)
                                                                                     tCCAATTGGAGCCTTTTTATGACTATTTATTATAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACt                                                              >  4:925100/1‑149 (MQ=255)
                                                                                     tCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGacac                                                                                                                             >  4:257743/1‑86 (MQ=255)
                                                                                     tCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGacac                                                                                                                             <  3:257743/86‑1 (MQ=255)
                                                                                     tCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACt                                                              >  4:211554/1‑149 (MQ=255)
                                                                                     tCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACt                                                              >  1:112659/1‑149 (MQ=255)
                                                                                       cAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                                                                                       <  1:49750/90‑1 (MQ=255)
                                                                                       cAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                                                                                       >  2:49750/1‑90 (MQ=255)
                                                                                       cAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGc                                                            <  3:970400/149‑1 (MQ=255)
                                                                                               gCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTg                                                    >  4:805952/1‑149 (MQ=255)
                                                                                                ccTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGa                                                   <  4:401028/149‑1 (MQ=255)
                                                                                                 cTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAg                                                  >  4:862498/1‑149 (MQ=255)
                                                                                                 cTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAg                                                  <  2:493934/149‑1 (MQ=255)
                                                                                                 cTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAg                                                  >  3:672682/1‑149 (MQ=255)
                                                                                                  tttttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGc                                                 >  1:582409/1‑149 (MQ=255)
                                                                                                  tttttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCATTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGc                                                 >  4:611088/1‑149 (MQ=255)
                                                                                                   ttttATGACTATTTATTAAAATATCCTACGTCACGCCTCTGTAGAAAGCAGGCTTGAGCATGAAGCTGACACTCATACCTGCCTTAAAGTGGCGTTTTTGTCGTTTCCCCTACCTACATTTACTGTTTTTTAACGGCCAGAACTGAGCg                                                <  4:947413/149‑1 (MQ=255)
                                                                                                   ttttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCg                                                <  1:993153/149‑1 (MQ=255)
                                                                                                   ttttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCg                                                <  3:212577/149‑1 (MQ=255)
                                                                                                     ttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGt                                              <  4:577343/149‑1 (MQ=255)
                                                                                                        tGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGa                                           >  2:824025/1‑149 (MQ=255)
                                                                                                         gACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGaa                                          >  1:44392/1‑149 (MQ=255)
                                                                                                           cTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGaaaa                                        <  3:760314/149‑1 (MQ=255)
                                                                                                             atttattAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGc                                      <  1:717550/149‑1 (MQ=255)
                                                                                                             atttattAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGc                                      <  2:735939/149‑1 (MQ=255)
                                                                                                                  ttaAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGaa                                 <  2:928476/149‑1 (MQ=255)
                                                                                                                  ttaAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGaa                                 <  4:672682/149‑1 (MQ=255)
                                                                                                                   taAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGaaa                                >  2:689788/1‑149 (MQ=255)
                                                                                                                     aaaTATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAata                              >  4:925182/1‑149 (MQ=255)
                                                                                                                     aaaTATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAata                              <  4:69129/149‑1 (MQ=255)
                                                                                                                     aaaTATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAata                              <  2:452002/149‑1 (MQ=255)
                                                                                                                      aaTATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAACGCCAGAAatat                             <  3:611088/149‑1 (MQ=255)
                                                                                                                        tatCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGCACTGAGCGGTTGAAAAGCCAGAAATATGc                           <  3:925100/149‑1 (MQ=255)
                                                                                                                           ccTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGa                        >  1:1100910/1‑149 (MQ=255)
                                                                                                                           ccTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGATACCCATACCTATATTTTCTGTAGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGa                        <  1:524860/149‑1 (MQ=255)
                                                                                                                            cTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATAc                                                                                    >  1:394015/1‑88 (MQ=255)
                                                                                                                            cTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCtgt                                                                       >  4:934121/1‑101 (MQ=255)
                                                                                                                            cTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAGCTGAGCGGTTGAAAAGCCAGAAATATGCCGAg                       >  1:205233/1‑149 (MQ=255)
                                                                                                                            cTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAg                       >  2:914552/1‑149 (MQ=255)
                                                                                                                            cTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCCATATTTTCtgt                                                                       <  3:934121/101‑1 (MQ=255)
                                                                                                                                 tcaaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGTCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCa                                    >  1:478327/3‑131 (MQ=255)
                                                                                                                                 tcaaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGTCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCa                                    <  2:478327/129‑1 (MQ=255)
                                                                                                                                 tcaaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGa                                                   <  4:197367/114‑1 (MQ=255)
                                                                                                                                   aaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCgg                >  3:506503/1‑149 (MQ=255)
                                                                                                                                       cTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATg            >  1:916077/1‑149 (MQ=255)
                                                                                                                                        tGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGACAGAAATATGCCGAGCCAGCGGCATgg           >  4:344288/1‑149 (MQ=255)
                                                                                                                                          tttttAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGaa         >  3:744832/1‑149 (MQ=255)
                                                                                                                                          tttttAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCACGGaa         <  1:5058/149‑1 (MQ=255)
                                                                                                                                            tttAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAgc       <  2:385419/149‑1 (MQ=255)
                                                                                                                                            tttAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAgc       <  2:649644/149‑1 (MQ=255)
                                                                                                                                                aaGCAGGCTTGAGCGTGAAGCGGACGCTCATACCTGCTTTATAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCca   >  3:347835/1‑149 (MQ=255)
                                                                                                                                                aaGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAccc                                                                                        <  4:73604/64‑1 (MQ=255)
                                                                                                                                                aaGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAccc                                                                                        >  3:73604/1‑64 (MQ=255)
                                                                                                                                                aaGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCca   >  4:472575/1‑149 (MQ=255)
                                                                                                                                                 aGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCcag  >  1:1021494/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCCAG  >  NC_000913/2774947‑2775240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: