Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,814,175 T→A 6.2% intergenic (+21/+43) emrB → / ← luxS multidrug efflux system protein/S‑ribosylhomocysteine lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,814,1750TA 6.2% 389.3 / 12.6 114intergenic (+21/+43)emrB/luxSmultidrug efflux system protein/S‑ribosylhomocysteine lyase
Reads supporting (aligned to +/- strand):  ref base T (51/55);  new base A (4/3);  total (55/59)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

Only 100 of 116 total aligned reads displayed.

GGGGCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTC  >  NC_000913/2814028‑2814322
                                                                                                                                                   |                                                                                                                                                   
   gCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTGCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGaa                                                                                                                                                 >  3:488678/1‑149 (MQ=255)
   gCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGaa                                                                                                                                                 <  3:808070/149‑1 (MQ=255)
    cTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAAc                                                                                                                                                <  3:849752/149‑1 (MQ=255)
      gATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTg                                                                                                                                              >  3:981906/1‑149 (MQ=255)
       attattTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTgg                                                                                                                                             >  3:51275/1‑149 (MQ=255)
        ttattTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGc                                                                                                                                            <  4:113330/149‑1 (MQ=255)
           tttCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCttt                                                                                                                                         >  1:975447/1‑149 (MQ=255)
              ccGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCtttttt                                                                                                                                      <  2:1027520/149‑1 (MQ=255)
               cGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTc                                                                                                                                     >  4:259335/1‑149 (MQ=255)
                   aaTGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaatt                                                                                                                                 >  2:112488/1‑149 (MQ=255)
                       agaTCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaattaatt                                                                                                                             <  3:894165/149‑1 (MQ=255)
                        gaTCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATtg                                                                                                                            >  1:447624/1‑149 (MQ=255)
                         aTCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATtgt                                                                                                                           >  2:738299/1‑149 (MQ=255)
                            ttctGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGaa                                                                                                                        <  4:58224/149‑1 (MQ=255)
                                ggATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATa                                                                                                                    >  4:47578/1‑149 (MQ=255)
                                 gATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAg                                                                                                                   <  2:413687/149‑1 (MQ=255)
                                   tGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGtt                                                                                                                 <  1:422276/149‑1 (MQ=255)
                                       aGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACt                                                                                                             >  4:57257/1‑149 (MQ=255)
                                       aGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACt                                                                                                             >  3:195100/1‑149 (MQ=255)
                                          cGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGAc                                                                                                          <  3:57257/149‑1 (MQ=255)
                                            ggATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTa                                                                                                        <  3:242671/149‑1 (MQ=255)
                                             gATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAg                                                                                                       >  4:175274/1‑149 (MQ=255)
                                              atatTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGa                                                                                                      >  1:473351/1‑149 (MQ=255)
                                               tatTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAt                                                                                                     >  1:903007/1‑149 (MQ=255)
                                                atTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTGACTGACTAGAt                                                                                                     <  3:632450/148‑1 (MQ=255)
                                                 ttCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtgt                                                                                                   <  4:488678/149‑1 (MQ=255)
                                                   ccTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAACCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGc                                                                                                 <  4:1036264/149‑1 (MQ=255)
                                                    cTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa                                                                                                >  2:334642/1‑149 (MQ=255)
                                                    cTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa                                                                                                >  4:454230/1‑149 (MQ=255)
                                                    cTCGTCCTGCTCGGGCTGGTGTGGTTAGCGAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTATGTACGACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAAGTGTGAAGATAGTATGCTGATTAGATGTGCa                                                                                                <  4:668942/149‑1 (MQ=255)
                                                     tCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAg                                                                                               >  1:228186/1‑149 (MQ=255)
                                                     tCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCACACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAg                                                                                               <  1:1060003/149‑1 (MQ=255)
                                                         ccTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc                                                                                           <  1:669234/149‑1 (MQ=255)
                                                         ccTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc                                                                                           <  1:1054064/149‑1 (MQ=255)
                                                          ctgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCt                                                                                          <  2:975447/149‑1 (MQ=255)
                                                          ctgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCt                                                                                          <  2:977265/149‑1 (MQ=255)
                                                          ctgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCt                                                                                          <  3:100889/149‑1 (MQ=255)
                                                            gctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc                                                                                        <  2:277971/149‑1 (MQ=255)
                                                            gctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc                                                                                        >  1:484956/1‑149 (MQ=255)
                                                                  gCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAg                                                                                                                   >  2:1014145/1‑116 (MQ=255)
                                                                  gCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAg                                                                                                                   <  1:1014145/116‑1 (MQ=255)
                                                                  gCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc                                                                                  <  2:1063065/149‑1 (MQ=255)
                                                                  gCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc                                                                                  >  3:514916/1‑149 (MQ=255)
                                                                       tgtgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCttt                                                                             >  2:1125/1‑149 (MQ=255)
                                                                       tgtgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCttt                                                                             >  3:804289/1‑149 (MQ=255)
                                                                        gtgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTc                                                                            <  1:220258/149‑1 (MQ=255)
                                                                         tgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                                                                           <  1:649156/149‑1 (MQ=255)
                                                                         tgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                                                                           >  1:1086638/1‑149 (MQ=255)
                                                                          ggTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCgg                                                                          <  4:649038/149‑1 (MQ=255)
                                                                            tttGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCa                                                                        <  4:418581/149‑1 (MQ=255)
                                                                               gCTAGACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGGGCGCACTAAGTAAAACTAAGCCAGTTCATGTGAACGGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTg                                                                     <  2:270799/149‑1 (MQ=255)
                                                                                 tAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGcc                                                                   >  1:416801/1‑149 (MQ=255)
                                                                                  aaaCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCa                                                                  >  4:919761/1‑149 (MQ=255)
                                                                                  aaaCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCa                                                                  >  1:1089663/1‑149 (MQ=255)
                                                                                  aaaCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCa                                                                  <  1:193866/149‑1 (MQ=255)
                                                                                   aaCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAg                                                                 <  1:178093/149‑1 (MQ=255)
                                                                                    aCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                                                                           >  2:878207/1‑138 (MQ=255)
                                                                                    aCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                                                                           <  1:878207/138‑1 (MQ=255)
                                                                                      cGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                                                                           >  1:22667/1‑136 (MQ=255)
                                                                                      cGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                                                                           <  2:22667/136‑1 (MQ=255)
                                                                                      cGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttc                                                              >  4:141138/1‑149 (MQ=255)
                                                                                      cGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttc                                                              >  2:470946/1‑149 (MQ=255)
                                                                                         cATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTAc                                                                                                              <  4:569614/98‑1 (MQ=255)
                                                                                         cATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTAc                                                                                                              >  3:569614/1‑98 (MQ=255)
                                                                                           tttGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGt                                                         <  2:447624/149‑1 (MQ=255)
                                                                                               gcgcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCt                                                     <  3:114728/149‑1 (MQ=255)
                                                                                               gcgcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCt                                                     <  2:866537/149‑1 (MQ=255)
                                                                                                 gcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAAGTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGt                                                   <  3:1029219/149‑1 (MQ=255)
                                                                                                  cAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt                                                  <  4:248936/149‑1 (MQ=255)
                                                                                                       ggcggcGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGt                                                         >  1:737221/1‑137 (MQ=255)
                                                                                                          ggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTa                                          >  1:186444/1‑149 (MQ=255)
                                                                                                            cggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGaa                                                                                                                        >  2:356947/1‑69 (MQ=255)
                                                                                                             ggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAg                                                                       <  2:834093/117‑1 (MQ=255)
                                                                                                             ggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAg                                                                       >  1:834093/1‑117 (MQ=255)
                                                                                                              gAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTc                                      <  4:349189/149‑1 (MQ=255)
                                                                                                                     tGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTcc                               >  4:816895/1‑149 (MQ=255)
                                                                                                                     tGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTcc                               <  3:244171/149‑1 (MQ=255)
                                                                                                                        gcACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGa                            >  3:11689/1‑149 (MQ=255)
                                                                                                                         cACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTTACTAGATGTGCAGTTCCTTCAACTTCTCTTTCGGCAGTTCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGaa                           <  3:565418/149‑1 (MQ=255)
                                                                                                                         cACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGaa                           <  1:107469/149‑1 (MQ=255)
                                                                                                                            tAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGc                        >  4:1043389/1‑149 (MQ=255)
                                                                                                                            tAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGc                        >  3:966630/1‑149 (MQ=255)
                                                                                                                             aaGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCt                       <  1:470946/149‑1 (MQ=255)
                                                                                                                              aGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTa                      >  3:869529/1‑149 (MQ=255)
                                                                                                                                tACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTAcg                    >  2:987210/1‑149 (MQ=255)
                                                                                                                                 aCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc                                                                                        >  3:434686/1‑80 (MQ=255)
                                                                                                                                 aCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTAcgc                   >  1:64920/1‑149 (MQ=255)
                                                                                                                                    aCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCa                >  3:681830/1‑149 (MQ=255)
                                                                                                                                    aCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCa                >  1:735893/1‑149 (MQ=255)
                                                                                                                                    aCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCa                >  2:714561/1‑149 (MQ=255)
                                                                                                                                        aGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGa                                                <  4:408859/113‑1 (MQ=255)
                                                                                                                                        aGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGa                                                >  3:408859/1‑113 (MQ=255)
                                                                                                                                         gCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATc           >  4:708672/1‑149 (MQ=255)
                                                                                                                                          ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATcc          <  4:11689/149‑1 (MQ=255)
                                                                                                                                           cAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCt         <  2:228186/149‑1 (MQ=255)
                                                                                                                                             gTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTgc       >  1:1024549/1‑149 (MQ=255)
                                                                                                                                                cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCt    <  1:738299/149‑1 (MQ=255)
                                                                                                                                                cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCt    <  4:780789/149‑1 (MQ=255)
                                                                                                                                                 aTTTGATCTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGCCTAGATGTGCAGTTCCTGCAACTTGTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTGCGTCACGTTCCAGAATCCTACGCGCAATATCCTGCGCtt   >  2:794740/1‑149 (MQ=255)
                                                                                                                                                  tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTc  >  2:874394/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGGGCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTC  >  NC_000913/2814028‑2814322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: