Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,842,437 G→T 5.3% intergenic (+23/+136) ascB → / ← hycI cryptic 6‑phospho‑beta‑glucosidase/protease involved in processing C‑terminal end of HycE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,842,4370GT 5.3% 399.2 / 9.1 114intergenic (+23/+136)ascB/hycIcryptic 6‑phospho‑beta‑glucosidase/protease involved in processing C‑terminal end of HycE
Reads supporting (aligned to +/- strand):  ref base G (57/51);  new base T (4/2);  total (61/53)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 114 total aligned reads displayed.

AACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTC  >  NC_000913/2842290‑2842584
                                                                                                                                                   |                                                                                                                                                   
aaCGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGa                                                                                                                                                    >  1:370292/1‑149 (MQ=255)
  cGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAAc                                                                                                                                                  <  3:163386/149‑1 (MQ=255)
    cTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGc                                                                                                                                                >  1:732575/1‑149 (MQ=255)
     tACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACTGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGcc                                                                                                                                               >  4:1001178/1‑149 (MQ=255)
     tACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGcc                                                                                                                                               >  4:797379/1‑149 (MQ=255)
       cGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCtt                                                                                                                                             <  4:887266/149‑1 (MQ=255)
       cGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCtt                                                                                                                                             <  4:357428/149‑1 (MQ=255)
       cGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATAAGGCGTTCACGccgc                                                                                                                                             <  1:1089513/149‑3 (MQ=255)
         gCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                           >  1:16085/1‑149 (MQ=255)
          ctttgtctttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                          <  2:1032137/149‑1 (MQ=255)
            ttgtctttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                                        <  2:729978/149‑1 (MQ=255)
            ttgtctttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                                        <  4:596529/149‑1 (MQ=255)
               tctttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                     <  4:307568/149‑1 (MQ=255)
               tctttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                     >  3:162205/1‑149 (MQ=255)
               tctttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                     >  4:1059259/1‑149 (MQ=255)
                ctttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                                                                                                                    >  1:314963/1‑149 (MQ=255)
                 tttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                                        >  2:666207/1‑144 (MQ=255)
                 tttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                                        <  1:666207/144‑1 (MQ=255)
                 tttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa                                                                                                                                   >  3:49761/1‑149 (MQ=255)
                 tttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa                                                                                                                                   >  1:280550/1‑149 (MQ=255)
                 tttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa                                                                                                                                   >  1:216702/1‑149 (MQ=255)
                 tttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                                       >  1:145416/1‑145 (MQ=255)
                 tttgtTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                                       <  2:145416/145‑1 (MQ=255)
                     ttgACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATg                                                                                                                               >  4:735450/1‑149 (MQ=255)
                     ttgACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATg                                                                                                                               >  1:287259/1‑149 (MQ=255)
                                gacgCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                     >  4:404035/1‑132 (MQ=255)
                                gacgCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                     <  3:404035/132‑1 (MQ=255)
                                  cgCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCAt                                                                                                                  >  3:452295/1‑149 (MQ=255)
                                  cgCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCAt                                                                                                                  <  4:902764/149‑1 (MQ=255)
                                  cgCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCAt                                                                                                                  <  4:890936/149‑1 (MQ=255)
                                   gCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATg                                                                                                                 >  2:547305/1‑149 (MQ=255)
                                    cAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGa                                                                                                                <  3:797379/149‑1 (MQ=255)
                                      ggCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAg                                                                                                              >  2:111415/1‑149 (MQ=255)
                                      ggCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAg                                                                                                              <  2:501015/149‑1 (MQ=255)
                                       gCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAgg                                                                                                             >  3:562668/1‑149 (MQ=255)
                                         aaCGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGcc                                                                                                           >  4:953786/1‑149 (MQ=255)
                                          aCGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGccc                                                                                                          <  4:630945/149‑1 (MQ=255)
                                            ggTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGa                                                                                                        >  4:589983/1‑149 (MQ=255)
                                               aCGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATaa                                                                                                     <  3:1049073/149‑1 (MQ=255)
                                                 gCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAagc                                                                                                   >  2:845257/1‑149 (MQ=255)
                                                     aCGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAagcgcag                                                                                               <  3:589983/149‑1 (MQ=255)
                                                           aCGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATc                                                                                         >  4:468883/1‑149 (MQ=255)
                                                             gcgTAAGAAATCATTCTGGTTGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCgg                                                                                       <  1:599033/149‑1 (MQ=255)
                                                             gcgTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCgg                                                                                       <  4:68508/149‑1 (MQ=255)
                                                               gTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGc                                                                                     >  4:407827/1‑149 (MQ=255)
                                                                tAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCa                                                                                    >  1:827138/1‑149 (MQ=255)
                                                                  aGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAAt                                                                                  >  3:545953/1‑149 (MQ=255)
                                                                   gAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGTAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAAtt                                                                                 >  3:944435/1‑149 (MQ=255)
                                                                   gAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGACCGATAAGCGCAGCGCATCGGACAAtt                                                                                 >  4:935028/1‑149 (MQ=255)
                                                                     aaTCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTa                                                                               <  3:450558/149‑1 (MQ=255)
                                                                     aaTCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTa                                                                               >  4:102009/1‑149 (MQ=255)
                                                                      aTCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAg                                                                              >  3:215030/1‑149 (MQ=255)
                                                                      aTCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAg                                                                              >  3:869572/1‑149 (MQ=255)
                                                                       tCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGc                                                                             >  2:243100/1‑149 (MQ=255)
                                                                         aTTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCAt                                                                           >  2:325491/1‑149 (MQ=255)
                                                                          ttCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCa                                                                            >  2:165618/1‑147 (MQ=255)
                                                                          ttCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCa                                                                            <  1:165618/147‑1 (MQ=255)
                                                                            cTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTg                                                                        <  2:287259/149‑1 (MQ=255)
                                                                               gtggtATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCa                                                                     >  4:778147/1‑149 (MQ=255)
                                                                                   tataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCag                                                                 >  4:816080/1‑149 (MQ=255)
                                                                                    ataAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCaga                                                                <  4:601764/149‑1 (MQ=255)
                                                                                     taAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATaa                                                                                                     <  3:99971/111‑1 (MQ=255)
                                                                                      aaaaaaGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGATCCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGc                                                              >  1:488712/1‑149 (MQ=255)
                                                                                      aaaaaaGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGc                                                              <  3:740703/149‑1 (MQ=255)
                                                                                       aaaaaGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCt                                                             >  4:743276/1‑149 (MQ=255)
                                                                                        aaaaGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTg                                                            >  4:454113/1‑149 (MQ=255)
                                                                                        aaaaGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTg                                                            >  1:781788/1‑149 (MQ=255)
                                                                                        aaaaGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTg                                                            >  4:639250/1‑149 (MQ=255)
                                                                                        aaaaGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTg                                                            <  3:953786/149‑1 (MQ=255)
                                                                                        aaaaGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTg                                                            <  3:724646/149‑1 (MQ=255)
                                                                                          aaGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGt                                                          <  2:216702/149‑1 (MQ=255)
                                                                                            gTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTc                                                        <  4:562668/149‑1 (MQ=255)
                                                                                               aTTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCAcc                                                     <  3:517244/149‑1 (MQ=255)
                                                                                                ttGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCAccc                                                    >  3:799069/1‑149 (MQ=255)
                                                                                                      gTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCaa                                              <  2:370292/149‑1 (MQ=255)
                                                                                                            gggTAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCCAGAACAt                                        >  4:896284/1‑149 (MQ=255)
                                                                                                            gggAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACAt                                        >  1:600360/1‑149 (MQ=255)
                                                                                                             ggAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATc                                       >  3:704948/1‑149 (MQ=255)
                                                                                                              gAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATcc                                      >  4:197103/1‑149 (MQ=255)
                                                                                                              gAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATcc                                      <  2:103536/149‑1 (MQ=255)
                                                                                                                aGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCt                                    <  2:346183/149‑1 (MQ=255)
                                                                                                                   tttAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCATGTc                                 >  4:396720/1‑149 (MQ=255)
                                                                                                                     tAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCt                               <  4:944435/149‑1 (MQ=255)
                                                                                                                     tAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCt                               <  3:223230/149‑1 (MQ=255)
                                                                                                                     tAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCt                               <  4:704948/149‑1 (MQ=255)
                                                                                                                       gagTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGa                             <  3:735450/149‑1 (MQ=255)
                                                                                                                         gTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGAtt                           <  1:420867/149‑1 (MQ=255)
                                                                                                                           aGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCAGATTcc                         <  2:781788/149‑1 (MQ=255)
                                                                                                                             gTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATcc                                      >  2:472215/1‑134 (MQ=255)
                                                                                                                             gTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATcc                                      <  1:472215/134‑1 (MQ=255)
                                                                                                                               aaCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTaa                     <  2:600360/149‑1 (MQ=255)
                                                                                                                               aaCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTaa                     <  3:1059259/149‑1 (MQ=255)
                                                                                                                                 cAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATg                   <  2:280550/149‑1 (MQ=255)
                                                                                                                                    ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGaaa                >  3:574497/2‑149 (MQ=255)
                                                                                                                                    ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGaaa                >  1:790384/2‑149 (MQ=255)
                                                                                                                                         gATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAacta           >  2:397896/1‑149 (MQ=255)
                                                                                                                                            gcggcgTGAACGCCTTATCCGGCCTACATGTCGCACGATCCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTActc        <  2:488712/149‑1 (MQ=255)
                                                                                                                                            gcggcgTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTActc        <  2:732575/149‑1 (MQ=255)
                                                                                                                                                  tGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTaa                     <  1:522360/130‑1 (MQ=255)
                                                                                                                                                  tGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCACTGTCCTGATTCCTTAATGAAAAACTACTCttcttc  <  4:545953/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AACGCTACGGCTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGTACGCTGACGCGCACGCGTAAGAAATCATTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAGGTAACAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTC  >  NC_000913/2842290‑2842584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: