Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,972,652 A→T 6.9% intergenic (+15/+17) tas → / ← lplT putative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,972,6520AT 6.9% 443.0 / 16.0 128intergenic (+15/+17)tas/lplTputative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter
Reads supporting (aligned to +/- strand):  ref base A (63/56);  new base T (5/4);  total (68/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 131 total aligned reads displayed.

CAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACAT  >  NC_000913/2972505‑2972800
                                                                                                                                                   |                                                                                                                                                    
 aGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTaaa                                                                                                                                                    <  1:166181/149‑1 (MQ=255)
      tctGCTGGGCGCCACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCgg                                                                                                                                               >  3:259442/1‑149 (MQ=255)
      tctGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCgg                                                                                                                                               >  2:61187/1‑149 (MQ=255)
      tctGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCgg                                                                                                                                               >  2:514892/1‑149 (MQ=255)
      tctGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCgg                                                                                                                                               >  3:1043779/1‑149 (MQ=255)
          ctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGtt                                                                                                                                           <  2:479310/149‑1 (MQ=255)
          ctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAACACCGGCGtt                                                                                                                                           <  2:881562/149‑1 (MQ=255)
              gcgcAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAat                                                                                                                                       >  3:749910/1‑149 (MQ=255)
                   aCCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAGCCGGCGTTAAATATTaa                                                                                                                                  <  3:57090/149‑1 (MQ=255)
                      aCGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGc                                                                                                                                              >  2:95830/1‑134 (MQ=255)
                      aCGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGa                                                                                                                               >  4:54896/1‑149 (MQ=255)
                      aCGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGa                                                                                                                               >  3:788587/1‑149 (MQ=255)
                        gATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACg                                                                                                                             >  2:1067847/1‑149 (MQ=255)
                            gATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGgcgc                                                                                                                         >  1:852791/1‑149 (MQ=255)
                             aTCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTCAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGGTAAAACCGGCGTTAAATATTAATGACGGCGCt                                                                                                                        >  2:498648/1‑149 (MQ=255)
                                aGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGcc                                                                                                                     >  1:318076/1‑149 (MQ=255)
                                   tGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGt                                                                                                                                            >  4:710405/1‑123 (MQ=255)
                                   tGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGt                                                                                                                                            <  3:710405/123‑1 (MQ=255)
                                   tGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGa                                                                                                                  >  1:170119/1‑149 (MQ=255)
                                   tGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGa                                                                                                                  >  1:336604/1‑149 (MQ=255)
                                    gAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGAt                                                                                                                 >  2:369839/1‑149 (MQ=255)
                                     aaaaCTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATc                                                                                                                <  1:338065/149‑1 (MQ=255)
                                       aaCTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTaa                                                                                                                                  <  3:398059/129‑1 (MQ=255)
                                       aaCTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTaa                                                                                                                                  >  4:398059/1‑129 (MQ=255)
                                          tAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAg                                                                                                           >  4:52949/1‑149 (MQ=255)
                                              aTCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGtt                                                                                                                                           >  4:194406/1‑113 (MQ=255)
                                              aTCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGtt                                                                                                                                           <  3:194406/113‑1 (MQ=255)
                                              aTCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCcaca                                                                                                            <  2:1011790/144‑1 (MQ=255)
                                              aTCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCcaca                                                                                                            >  1:1011790/1‑144 (MQ=255)
                                               tCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCg                                                                                                      >  3:344529/1‑149 (MQ=255)
                                               tCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCg                                                                                                      >  2:336179/1‑149 (MQ=255)
                                                  aaaGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAATCCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTa                                                                                                   >  4:961105/1‑149 (MQ=255)
                                                    aGTTTGCATCTGGAGTTAAGCGAAGTCGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGGCGGTTAAGACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTAtt                                                                                                 <  4:769039/149‑1 (MQ=255)
                                                    aGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTAtt                                                                                                 <  2:371317/149‑1 (MQ=255)
                                                    aGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTAtt                                                                                                 <  4:344529/149‑1 (MQ=255)
                                                    aGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTAtt                                                                                                 >  4:423163/1‑149 (MQ=255)
                                                     gTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTg                                                                                                >  3:458981/1‑149 (MQ=255)
                                                        tGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAatat                                                                                                                                     <  4:91048/109‑1 (MQ=255)
                                                         gCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAg                                                                                            <  4:786832/149‑1 (MQ=255)
                                                          cATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGTTa                                                                                                                                          <  4:1025411/102‑1 (MQ=255)
                                                          cATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGCTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGTTa                                                                                                                                          >  3:1025411/1‑102 (MQ=255)
                                                            tCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGACAGATCCACAGCGCCGTTATTGCCAgcgc                                                                                         >  2:775411/1‑149 (MQ=255)
                                                               ggAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCaaa                                                                                      <  2:942120/149‑1 (MQ=255)
                                                               ggAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCaaa                                                                                      <  2:104619/149‑1 (MQ=255)
                                                               ggAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCaaa                                                                                      >  4:27835/1‑149 (MQ=255)
                                                                 aGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACa                                                                                    >  1:523574/1‑149 (MQ=255)
                                                                  gTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGa                                                                                                                               >  4:1073406/1‑105 (MQ=255)
                                                                  gTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAg                                                                                            <  4:346305/140‑1 (MQ=255)
                                                                   ttAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAgc                                                                                  >  4:317333/1‑149 (MQ=255)
                                                                      aGCGAAGACGTATTGGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCa                                                                               >  3:78266/1‑149 (MQ=255)
                                                                      aGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCa                                                                               >  3:576668/1‑149 (MQ=255)
                                                                         gAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGAt                                                                                                                 >  3:13832/1‑112 (MQ=255)
                                                                         gAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGAt                                                                                                                 <  4:13832/112‑1 (MQ=255)
                                                                         gAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCg                                                                            >  1:891419/1‑149 (MQ=255)
                                                                         gAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCg                                                                            >  2:132115/1‑149 (MQ=255)
                                                                           aGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGaa                                                                          <  2:210971/149‑1 (MQ=255)
                                                                             aCGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgc                                                                        <  4:167982/149‑1 (MQ=255)
                                                                             aCGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACAATAGGATTAACGCCGGTTAAAACCGGCGTCAAATATTAATGACGGAGCTGCCAGATCCACAGCGACGTTATTGCCAGCGCAAACAGCGCACCGAAgc                                                                        >  4:272943/1‑149 (MQ=255)
                                                                               gTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgcca                                                                      >  3:107308/1‑149 (MQ=255)
                                                                               gTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgcca                                                                      >  1:302320/1‑149 (MQ=255)
                                                                                 aTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaat                                                                    <  3:54896/149‑1 (MQ=255)
                                                                                 aTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaat                                                                    <  2:852791/149‑1 (MQ=255)
                                                                                 aTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaat                                                                    <  1:936448/149‑1 (MQ=255)
                                                                                       tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGt                                                                                                     >  3:267667/1‑110 (MQ=255)
                                                                                       tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaatgccaat                                                              <  3:908560/149‑1 (MQ=255)
                                                                                        gAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGccaatgccaatg                                                             >  1:38093/1‑149 (MQ=255)
                                                                                        gAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGccaatgccaatg                                                             >  1:770083/1‑149 (MQ=255)
                                                                                         aaaTTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaa                                                                     >  2:907484/1‑140 (MQ=255)
                                                                                         aaaTTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaa                                                                     <  1:907484/140‑1 (MQ=255)
                                                                                         aaaTTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATgg                                                            <  2:109511/149‑1 (MQ=255)
                                                                                           aTTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGc                                                          >  4:44163/1‑149 (MQ=255)
                                                                                           aTTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGc                                                          >  4:804158/1‑149 (MQ=255)
                                                                                            ttGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaatgcc                                                                 <  4:1037656/141‑1 (MQ=255)
                                                                                               aaGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGa                                                      <  2:170119/149‑1 (MQ=255)
                                                                                                aGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGAc                                                     <  1:956463/149‑1 (MQ=255)
                                                                                                  cGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCg                                                   >  3:604399/1‑149 (MQ=255)
                                                                                                  cGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCg                                                   <  1:246676/149‑1 (MQ=255)
                                                                                                     tGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGa                                                >  3:397095/1‑149 (MQ=255)
                                                                                                             gTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATc                                        <  3:153542/149‑1 (MQ=255)
                                                                                                             gTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATc                                        <  3:950786/149‑1 (MQ=255)
                                                                                                                 ataCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTa                                    <  4:749910/149‑1 (MQ=255)
                                                                                                                 ataCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTa                                    >  1:888744/1‑149 (MQ=255)
                                                                                                                  taCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTAc                                   <  1:1067847/149‑1 (MQ=255)
                                                                                                                  taCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTAc                                   <  4:458981/149‑1 (MQ=255)
                                                                                                                    cTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAgc                                                                                  >  3:993055/1‑100 (MQ=255)
                                                                                                                                 aTAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCATGACCGGGATGCCTATCATTACCGCCAGCGAGt                        >  3:958137/1‑145 (MQ=255)
                                                                                                                                 aTAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGt                        <  4:958137/145‑1 (MQ=255)
                                                                                                                                  tAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATg                   >  2:701121/1‑149 (MQ=255)
                                                                                                                                  tAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATg                   >  4:516509/1‑149 (MQ=255)
                                                                                                                                     gATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGccc                <  1:1028847/149‑1 (MQ=255)
                                                                                                                                      aTTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCa               <  1:1037296/149‑1 (MQ=255)
                                                                                                                                        tAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGc             <  1:187983/149‑1 (MQ=255)
                                                                                                                                         aaCGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCa            >  4:1030937/1‑149 (MQ=255)
                                                                                                                                         aaCGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCa            >  4:34830/1‑149 (MQ=255)
                                                                                                                                          aCGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGAt                                               >  3:513383/1‑113 (MQ=255)
                                                                                                                                           cGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATc          <  2:97200/149‑1 (MQ=255)
                                                                                                                                             ccGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaa        <  1:508869/149‑1 (MQ=255)
                                                                                                                                             ccGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaa        <  4:78266/149‑1 (MQ=255)
                                                                                                                                                 ttAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCAGCaacaac    >  1:556335/1‑149 (MQ=255)
                                                                                                                                                   aaaaCCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACAt  >  3:1043453/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACAT  >  NC_000913/2972505‑2972800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: