Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,160,640 G→C 7.6% T169T (ACC→ACG ygiQ ← Radical SAM superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,160,6400GC 7.6% 413.4 / 21.0 118T169T (ACC→ACGygiQRadical SAM superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (49/60);  new base C (3/6);  total (52/66)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

Only 100 of 120 total aligned reads displayed.

GCGGATTTCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCATGACGTAAAC  >  NC_000913/3160493‑3160788
                                                                                                                                                   |                                                                                                                                                    
gcgGATTTCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCgg                                                                                                                                                     >  4:266512/1‑149 (MQ=255)
         aCTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCa                                                                                                                                            >  1:418444/1‑149 (MQ=255)
          cTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAg                                                                                                                                           >  3:801660/1‑149 (MQ=255)
           tAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGa                                                                                                                                          >  1:521322/1‑149 (MQ=255)
           tAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGa                                                                                                                                          <  3:309784/149‑1 (MQ=255)
            aaTTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCCGTACCGCGCatta                                                                                                                                         <  1:366718/149‑4 (MQ=255)
             aTTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACt                                                                                                                                        >  2:252127/1‑149 (MQ=255)
             aTTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCCGTACCGCGCattat                                                                                                                                        <  1:851930/149‑5 (MQ=255)
              ttGGCTCGCCCATCGCCAGACGATGCGCCACTTGCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTa                                                                                                                                       <  4:950342/149‑1 (MQ=255)
              ttGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTa                                                                                                                                       <  2:165668/149‑1 (MQ=255)
              ttGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTa                                                                                                                                       >  3:1076989/1‑149 (MQ=255)
              ttGACTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCCGTACCGCGCattatg                                                                                                                                       <  3:1051428/149‑6 (MQ=255)
                ggCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCCGTACCGCGCattatgat                                                                                                                                     <  2:640348/149‑8 (MQ=255)
                   tCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTc                                                                                                                                  >  4:966995/1‑149 (MQ=255)
                     gCCCATCGCCAGACGATGCGCCCCTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCaa                                                                                                                                <  1:593362/149‑1 (MQ=255)
                     gCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCaa                                                                                                                                >  1:445673/1‑149 (MQ=255)
                       ccATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATa                                                                                                                              >  2:161283/1‑149 (MQ=255)
                         aTCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATAcc                                                                                                                            >  3:718441/1‑149 (MQ=255)
                         aTCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCCGTACCGCGCattatg                                                                                                                                       >  1:1087228/1‑133 (MQ=255)
                         aTCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCCGTACCGCGCattatg                                                                                                                                       <  2:1087228/138‑6 (MQ=255)
                          tCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATAccg                                                                                                                           >  3:934164/1‑149 (MQ=255)
                            gCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATAccgcc                                                                                                                         >  4:720272/1‑149 (MQ=255)
                               aGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAg                                                                                                                      <  1:252127/149‑1 (MQ=255)
                               aGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAg                                                                                                                      <  1:839598/149‑1 (MQ=255)
                                gACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTc                                                                                                                                  <  1:164848/136‑1 (MQ=255)
                                 aCGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGa                                                                                                                    >  1:83963/1‑149 (MQ=255)
                                  cGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGAt                                                                                                                   <  3:311883/149‑1 (MQ=255)
                                  cGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGAt                                                                                                                   <  1:214343/149‑1 (MQ=255)
                                  cGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGAt                                                                                                                   <  4:391140/149‑1 (MQ=255)
                                   gATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATc                                                                                                                  >  2:835606/1‑149 (MQ=255)
                                     tGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCAc                                                                                                                <  1:1087407/149‑1 (MQ=255)
                                     tGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCAc                                                                                                                <  1:757705/149‑1 (MQ=255)
                                     tGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCAc                                                                                                                <  1:189084/149‑1 (MQ=255)
                                      gcgcCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCAcc                                                                                                               <  2:150020/149‑1 (MQ=255)
                                      gcgcCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCAcc                                                                                                               <  4:718441/149‑1 (MQ=255)
                                        gcCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCgg                                                                                                             >  3:248624/1‑149 (MQ=255)
                                         ccACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGt                                                                                                            >  2:1035310/1‑149 (MQ=255)
                                                 accaGCGTACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGa                                                                                                                                          >  2:597184/1‑111 (MQ=255)
                                                 accaGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCttt                                                                                                    <  4:1026304/149‑1 (MQ=255)
                                                 accaGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCCGTACCGCGCat                                                                                                                                           <  4:686791/110‑2 (MQ=255)
                                                 accaGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCCGTACCGCGCat                                                                                                                                           >  3:686791/1‑109 (MQ=255)
                                                   caGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTcc                                                                                                  >  4:1018696/1‑149 (MQ=255)
                                                        gACGCTCACCGATACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGAGCACCGGTACATCTTTCCACGcc                                                                                             >  3:612728/1‑149 (MQ=255)
                                                         aCGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCct                                                                                            <  2:974421/149‑1 (MQ=255)
                                                           gCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCctct                                                                                          >  4:571516/1‑149 (MQ=255)
                                                             tCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCttt                                                                                        >  1:1024911/1‑149 (MQ=255)
                                                               aCCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTAc                                                                                      >  2:578093/1‑149 (MQ=255)
                                                                 cGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACaa                                                                                    <  3:216379/149‑1 (MQ=255)
                                                                     aCCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCt                                                                                <  3:362164/149‑1 (MQ=255)
                                                                     aCCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCt                                                                                <  4:80298/149‑1 (MQ=255)
                                                                     aCCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCt                                                                                <  4:801660/149‑1 (MQ=255)
                                                                       cAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAg                                                                                                                      <  2:459586/109‑1 (MQ=255)
                                                                       cAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAg                                                                                                                      >  1:459586/1‑109 (MQ=255)
                                                                       cAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTgg                                                                              <  1:726643/149‑1 (MQ=255)
                                                                       cAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTgg                                                                              >  2:935448/1‑149 (MQ=255)
                                                                        aaaCATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACaa                                                                                    >  1:26596/1‑142 (MQ=255)
                                                                        aaaCATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACaa                                                                                    <  2:26596/142‑1 (MQ=255)
                                                                          aCATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGta                                                                           >  4:821993/1‑149 (MQ=255)
                                                                            aTCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGtata                                                                         <  3:266512/149‑1 (MQ=255)
                                                                               aGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCGCGCCTCTTTACAACGCTGGGTATAAAc                                                                      <  4:516919/149‑1 (MQ=255)
                                                                                 cATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCa                                                                    <  4:923709/149‑1 (MQ=255)
                                                                                   tGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAgt                                                                  <  1:835606/149‑1 (MQ=255)
                                                                                    gTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAgtg                                                                 >  2:35207/1‑149 (MQ=255)
                                                                                      cGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGtgtg                                                               <  2:355744/149‑1 (MQ=255)
                                                                                         cTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGgcg                                                            >  1:421385/1‑149 (MQ=255)
                                                                                          tttCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGgcgc                                                           >  4:887094/1‑149 (MQ=255)
                                                                                             cGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGAt                                                        <  1:578093/149‑1 (MQ=255)
                                                                                               aaTCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATcc                                                      <  2:953806/149‑1 (MQ=255)
                                                                                                   caccaGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGgc                                                  <  1:35207/149‑1 (MQ=255)
                                                                                                    accaGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTACACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGgcg                                                 >  2:909979/1‑149 (MQ=255)
                                                                                                       agctcagAACGGCGCACGGTATCGGACAAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCtt                                              <  4:934164/143‑1 (MQ=255)
                                                                                                       aGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTAGAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCtt                                              <  4:626260/149‑1 (MQ=255)
                                                                                                          aCGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTAcc                                           >  3:759837/1‑149 (MQ=255)
                                                                                                           cGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCCCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTAccc                                          >  4:75753/1‑149 (MQ=255)
                                                                                                            ggAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCcg                                         >  2:6457/1‑149 (MQ=255)
                                                                                                             gAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCcgc                                        >  2:468204/1‑149 (MQ=255)
                                                                                                               aCGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGa                                      <  4:1076989/149‑1 (MQ=255)
                                                                                                               aCGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGa                                      <  1:750461/149‑1 (MQ=255)
                                                                                                                   cgcACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGtt                                  >  1:697147/1‑149 (MQ=255)
                                                                                                                    gcACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTa                                 <  1:1032330/149‑1 (MQ=255)
                                                                                                                    gcACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTa                                 <  3:743030/149‑1 (MQ=255)
                                                                                                                         gTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGtt                                  >  1:734495/1‑143 (MQ=255)
                                                                                                                         gTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGtt                                  <  2:734495/143‑1 (MQ=255)
                                                                                                                           aTCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCg                          >  4:953166/1‑149 (MQ=255)
                                                                                                                            tCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCgg                                                    >  2:1019530/1‑122 (MQ=255)
                                                                                                                            tCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCgg                                                    <  1:1019530/122‑1 (MQ=255)
                                                                                                                              ggACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCgtgt                       >  1:905693/1‑149 (MQ=255)
                                                                                                                              ggACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCgtgt                       <  3:358121/149‑1 (MQ=255)
                                                                                                                                aCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCGCCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAATCAGTGTGTCGCGATCCGGGCGCTTACCCGCGGCGTTATCCGGCGTGTAg                     <  4:667871/149‑1 (MQ=255)
                                                                                                                                aCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAg                     <  4:511536/149‑1 (MQ=255)
                                                                                                                                aCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAg                     <  2:486350/149‑1 (MQ=255)
                                                                                                                                  cAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGc                   >  3:574420/1‑149 (MQ=255)
                                                                                                                                    gTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCAt                 >  4:474271/1‑149 (MQ=255)
                                                                                                                                     tAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATc                <  4:248624/149‑1 (MQ=255)
                                                                                                                                         cATAATGCGCGGTACGGCGCAGACTAGCCTGAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCa            <  3:645924/149‑1 (MQ=255)
                                                                                                                                          aTAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGTGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCAt           <  2:298121/149‑1 (MQ=255)
                                                                                                                                          aTAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCAt           <  3:821993/149‑1 (MQ=255)
                                                                                                                                            tctGCGCCGTACCGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCATGa         >  1:476494/3‑149 (MQ=255)
                                                                                                                                             aTGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCATGAc        <  2:445673/149‑1 (MQ=255)
                                                                                                                                                   ggTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCATGACGTAAAc  <  3:474271/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGGATTTCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCGACGTTATCCGGCGTGTAGGCATCGTCATGACGTAAAC  >  NC_000913/3160493‑3160788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: