Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,231,460 C→A 5.0% intergenic (+221/‑205) ygjK → / → fadH alpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,231,4600CA 5.0% 347.2 / 7.0 99intergenic (+221/‑205)ygjK/fadHalpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (40/54);  new base A (2/3);  total (42/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

Only 100 of 103 total aligned reads displayed.

ACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGC  >  NC_000913/3231312‑3231604
                                                                                                                                                    |                                                                                                                                                
aCGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATgc                                                                                                                                                  >  3:646012/1‑149 (MQ=255)
aCGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATgc                                                                                                                                                  <  2:241963/149‑1 (MQ=255)
aCGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATgc                                                                                                                                                  >  2:648732/1‑149 (MQ=255)
 ttctaatttagccgtcgccggagaaaagggaaaaggggagtgcggggtttgtgatgggattcaaaaagaatacgcccggtgataacgccggatgcgacgcttgtcgcgttttatccaggttacggaattgcacatgtagGGCGGATgcg                                                                                                                                                 <  3:443005‑M1/10‑1 (MQ=255)
     gCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGt                                                                                                                                             <  4:332236/149‑1 (MQ=255)
       aaGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTgg                                                                                                                                           >  3:514277/1‑149 (MQ=255)
        aGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGa                                                                                                                                          >  4:706081/1‑149 (MQ=255)
           gTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGc                                                                                                                                       >  1:193661/1‑149 (MQ=255)
           gTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCCCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGc                                                                                                                                       <  3:768351/149‑1 (MQ=255)
                aTCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTAt                                                                                                                                  <  3:762951/149‑1 (MQ=255)
                 tCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATc                                                                                                                                 <  3:918019/149‑1 (MQ=255)
                 gccggagaaaagggaaaaggggagtgcggggtttgtgatgggattcaaaaagaatacgcccggtgataacgccggatgcgacgcttgtcgcgttttatccaggttacggaattgcacatgtagGGCGGATGCGGCGTGGACGCCTTATc                                                                                                                                 >  4:443005‑M1/124‑149 (MQ=255)
                     gCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGcc                                                                                                                             >  3:1027480/1‑149 (MQ=255)
                     gCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGcc                                                                                                                             <  1:129473/149‑1 (MQ=255)
                        aCTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTa                                                                                                                          <  4:653874/149‑1 (MQ=255)
                           gCACAGCGCGACTGGCGGATGCGGCGTGAACGCCTTATCAGCCCGACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAt                                                                                                                       <  4:588760/149‑1 (MQ=255)
                             aCCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgt                                                                                                                     <  4:440/149‑1 (MQ=255)
                                 cgcCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCaa                                                                                                                 <  3:403055/149‑1 (MQ=255)
                                 cgcCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCaa                                                                                                                 <  1:933734/149‑1 (MQ=255)
                                 cgcCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCaa                                                                                                                 <  1:938677/149‑1 (MQ=255)
                                  gcCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAAt                                                                                                                >  1:994159/1‑149 (MQ=255)
                                     aCTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTcc                                                                                                             <  4:1071504/149‑1 (MQ=255)
                                     aCTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTcc                                                                                                             <  3:856391/149‑1 (MQ=255)
                                         gCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTaa                                                                                                         <  4:657216/149‑1 (MQ=255)
                                           ggATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAAcc                                                                                                       >  4:670164/1‑149 (MQ=255)
                                            gATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCt                                                                                                      <  1:953801/149‑1 (MQ=255)
                                            gATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCt                                                                                                      <  1:292821/149‑1 (MQ=255)
                                            gATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCt                                                                                                      <  4:585697/149‑1 (MQ=255)
                                              tGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTgg                                                                                                    <  1:794980/149‑1 (MQ=255)
                                               gcggcgTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGa                                                                                                   <  3:251453/149‑1 (MQ=255)
                                                    gttcaCGCCGCATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATaaaa                                                                                              <  3:195723/145‑1 (MQ=255)
                                                    gTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCAACTGGCGGATGCGGCGTGGACCCCTTATCCGCCCTACATTTGCAATTCCGTAACCTGGATaaaa                                                                                              >  2:875812/1‑149 (MQ=255)
                                                        aCGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAAcgcg                                                                                          >  2:589057/1‑149 (MQ=255)
                                                         cGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGa                                                                                         >  4:508099/1‑149 (MQ=255)
                                                         cGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGACCTACATGTGCAATTCCGGAACCTGAATTAATCGCGa                                                                                         <  2:502346/149‑1 (MQ=18)
                                                          gCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGAc                                                                                        >  1:125545/1‑149 (MQ=255)
                                                            cTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACaa                                                                                      <  4:322069/149‑1 (MQ=255)
                                                               aTCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCg                                                                                   <  4:829583/149‑1 (MQ=255)
                                                                    cccTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCa                                                                              >  4:238233/1‑149 (MQ=255)
                                                                          aTGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCt                                                                                                      >  3:343748/1‑119 (MQ=255)
                                                                          aTGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCt                                                                                                      <  4:343748/119‑1 (MQ=255)
                                                                          aTGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGc                                                                        <  4:986723/149‑1 (MQ=255)
                                                                          aTGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGc                                                                        >  1:380564/1‑149 (MQ=255)
                                                                            gtgtgtTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGt                                                                      <  2:151222/149‑1 (MQ=255)
                                                                            gtgtgtTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGt                                                                      <  4:970821/149‑1 (MQ=255)
                                                                            gtgtgtTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGt                                                                      >  1:150467/1‑149 (MQ=255)
                                                                               tgtTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGa                                                                                         >  2:863279/1‑127 (MQ=255)
                                                                               tgtTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGa                                                                                         <  1:863279/127‑1 (MQ=255)
                                                                               tgtTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTAt                                                                   >  3:33643/1‑149 (MQ=255)
                                                                                 ttCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCa                                                                 >  3:233876/1‑149 (MQ=255)
                                                                                    ccGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCg                                                              >  4:772239/1‑149 (MQ=255)
                                                                                    ccGTAGATCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCg                                                              >  3:1002510/1‑149 (MQ=255)
                                                                                     cGTAGGTCGGATAAGACGCGACAAGCGTCGCATCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCggg                                                            >  2:42361/1‑149 (MQ=255)
                                                                                       tAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCGCCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGc                                                           <  2:771090/149‑1 (MQ=255)
                                                                                       tAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGc                                                           <  1:630688/149‑1 (MQ=255)
                                                                                       tAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGc                                                           <  2:588693/149‑1 (MQ=255)
                                                                                             ggATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGtt                                                                     >  3:267095/1‑133 (MQ=255)
                                                                                             ggATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGtt                                                                     <  4:267095/133‑1 (MQ=255)
                                                                                              gATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCt                                                    >  2:770735/1‑149 (MQ=255)
                                                                                                tAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCttt                                                  <  3:670164/149‑1 (MQ=255)
                                                                                                 aaGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCtttt                                                 <  2:426875/149‑1 (MQ=255)
                                                                                                  aGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCttttt                                                <  1:770735/149‑1 (MQ=255)
                                                                                                  aGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCttttt                                                <  2:327575/149‑1 (MQ=255)
                                                                                                  aGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCttttt                                                <  4:947509/149‑1 (MQ=255)
                                                                                                  aGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCttttt                                                <  2:403799/149‑1 (MQ=255)
                                                                                                  aGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCttttt                                                <  1:1058727/149‑1 (MQ=255)
                                                                                                    aCGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATc                                                                  <  3:481873/129‑1 (MQ=255)
                                                                                                    aCGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATc                                                                  >  4:481873/1‑129 (MQ=255)
                                                                                                        gACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATcc                                          >  2:930212/1‑149 (MQ=255)
                                                                                                           aaGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCAt                                       >  2:71856/1‑149 (MQ=255)
                                                                                                           aaGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCAt                                       >  3:908852/1‑149 (MQ=255)
                                                                                                            aGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATc                                      <  1:795380/149‑1 (MQ=255)
                                                                                                              cGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATcac                                    >  1:656782/1‑149 (MQ=255)
                                                                                                                    aTCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAAccc                              >  3:444501/1‑149 (MQ=255)
                                                                                                                      ccGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCg                            <  4:1002510/149‑1 (MQ=255)
                                                                                                                        ggCATCTGCACCGCGCCACTGGCGGATGCGGCGGGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCa                          >  2:330235/1‑149 (MQ=255)
                                                                                                                         gCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTaa                                                                                                         <  1:425919/69‑1 (MQ=255)
                                                                                                                         gCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTaa                                                                                                         >  2:425919/1‑69 (MQ=255)
                                                                                                                         gCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCAc                         >  3:608173/1‑149 (MQ=255)
                                                                                                                         gCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCAc                         <  2:125545/149‑1 (MQ=255)
                                                                                                                           aTCTGCACCGTGTCACAGGCGGATGCGGCGTGGACGCCTTATCCGCTCTACTTGTGCAATTCCGTAAACTGGATAAAACGAGACAAGCGTCGCATCCGGCGTTATAACCGGGCGTATTCTTTTTGAATCCCACCACAAACCCCGCACTc                       <  2:419824/149‑1 (MQ=255)
                                                                                                                              tGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTcccc                    >  4:484412/1‑149 (MQ=255)
                                                                                                                               gCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCccct                   <  3:772239/149‑1 (MQ=255)
                                                                                                                                 aCCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCcccttt                 >  3:914402/1‑149 (MQ=255)
                                                                                                                                   cgcgCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCcccttttc               <  2:656782/149‑1 (MQ=255)
                                                                                                                                    gcgcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCcccttttcc              <  1:490346/149‑1 (MQ=255)
                                                                                                                                    gcgcCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCcccttttcc              <  1:149664/149‑1 (MQ=255)
                                                                                                                                       ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATcc                                          <  2:181716/118‑1 (MQ=255)
                                                                                                                                       ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATcc                                          >  1:181716/1‑118 (MQ=255)
                                                                                                                                       ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCccctcttccctt           <  2:965787/149‑1 (MQ=255)
                                                                                                                                        cACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCcccttttcccttt          >  3:27565/1‑149 (MQ=255)
                                                                                                                                        cACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCcccttttcccttt          >  4:959088/1‑149 (MQ=255)
                                                                                                                                         aCTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCcccttttccctttt         >  1:483649/1‑149 (MQ=255)
                                                                                                                                          agGGCGGATAAGGCGTCCACGCCGCATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGc                                                                        <  3:1021329‑M1/83‑1 (MQ=255)
                                                                                                                                          agGGCGGATAAGGCGTCCACGCCGCATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGc                                                                        >  4:1021329‑M1/3‑85 (MQ=255)
                                                                                                                                           gGGCGGATAAGGCGTCCACGCCGCATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCtttt                                                 >  3:202929‑M1/2‑107 (MQ=255)
                                                                                                                                           gGGCGGATAAGGCGTCCACGCCGCATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCtttt                                                 <  4:202929‑M1/106‑1 (MQ=255)
                                                                                                                                           gGGCGGATAAGGCGTCCACGCCGCATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTtct       <  2:789527‑M1/148‑1 (MQ=255)
                                                                                                                                            GGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTtctc      >  1:1073476‑M1/1‑149 (MQ=255)
                                                                                                                                             GCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTcc     >  3:238233‑M1/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGC  >  NC_000913/3231312‑3231604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: