Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,328,182 A→T 9.6% intergenic (+99/+57) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,328,1820AT 9.6% 450.5 / 28.8 140intergenic (+99/+57)yhbY/greARNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
Reads supporting (aligned to +/- strand):  ref base A (62/60);  new base T (9/4);  total (73/67)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 141 total aligned reads displayed.

TGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAT  >  NC_000913/3328034‑3328325
                                                                                                                                                    |                                                                                                                                                
tGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCa                                                                                                                                                  >  3:831402/1‑149 (MQ=255)
 ggTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCAt                                                                                                                                                 <  3:293885/149‑1 (MQ=255)
  gTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATa                                                                                                                                                >  3:622842/1‑149 (MQ=255)
      tttATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATCAAAGGCCGCATAGCgg                                                                                                                                           <  4:738128/149‑1 (MQ=255)
      tttATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCgg                                                                                                                                           >  1:728277/1‑149 (MQ=255)
      tttATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCgg                                                                                                                                           >  1:401098/1‑149 (MQ=255)
        tATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGcc                                                                                                                                         >  3:403179/1‑149 (MQ=255)
         aTCGCCCAACTAAAGAATGTAAAATCTGGCTGCCACACTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGTGGGGTTTTCCGCAGGCAAGAGAGCAAAATGCCACGCTCTGCTCGTTGATAAAAGGCCGCATAGCGGCCt                                                                                                                                        >  4:1023816/1‑149 (MQ=255)
           cGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttt                                                                                                                                      <  3:327300/149‑1 (MQ=255)
            gCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCtttt                                                                                                                                     <  3:354153/149‑1 (MQ=255)
              ccAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCTACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTc                                                                                                                                   <  2:154651/149‑1 (MQ=255)
              ccAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTc                                                                                                                                   >  2:810083/1‑149 (MQ=255)
                aaCTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct                                                                                                                                 >  3:830189/1‑149 (MQ=255)
                  cTAAAGAACGTAAAATCTCGCTGCCACACTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCcttt                                                                                                                               <  1:87818/149‑1 (MQ=255)
                   tAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttc                                                                                                                              <  2:850151/149‑1 (MQ=255)
                   tAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCtttt                                                                                                                                     <  3:406368/142‑1 (MQ=255)
                   tAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCtttt                                                                                                                                     >  4:406368/1‑142 (MQ=255)
                    aaaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttct                                                                                                                             <  2:937527/149‑1 (MQ=255)
                    aaaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttct                                                                                                                             <  2:330760/149‑1 (MQ=255)
                    aaaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttct                                                                                                                             <  1:963694/149‑1 (MQ=255)
                     aaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttctt                                                                                                                            >  3:574186/1‑149 (MQ=255)
                     aaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttctt                                                                                                                            >  2:180057/1‑149 (MQ=255)
                     aaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCcttt                                                                                                                               <  4:381466/146‑1 (MQ=255)
                     aaGAACGTAAAATCGCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttctt                                                                                                                            <  2:434221/149‑1 (MQ=255)
                       gAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttc                                                                                                                              >  1:845653/1‑145 (MQ=255)
                       gAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTa                                                                                                                          <  1:948700/149‑1 (MQ=255)
                       gAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTa                                                                                                                          >  3:839255/1‑149 (MQ=255)
                       gAACGTAAAATCTCGCTGCCACGCTAAGATTACCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTa                                                                                                                          <  1:788668/149‑1 (MQ=255)
                         aCGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACa                                                                                                                        <  4:67195/149‑1 (MQ=255)
                            tAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATa                                                                                                                     >  1:726248/1‑149 (MQ=255)
                            tAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATa                                                                                                                     >  2:969541/1‑149 (MQ=255)
                            tAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATa                                                                                                                     <  2:401098/149‑1 (MQ=255)
                               aaTCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACAt                                                                                                                  >  1:325189/1‑149 (MQ=255)
                                aTCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATc                                                                                                                 >  3:530868/1‑149 (MQ=255)
                                          cACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGa                                                                                                       <  1:853179/149‑1 (MQ=255)
                                           aCGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAg                                                                                                      <  1:432334/149‑1 (MQ=255)
                                               tAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTAtt                                                                                                  >  3:48266/1‑149 (MQ=255)
                                               tAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTAtt                                                                                                  <  2:712568/149‑1 (MQ=255)
                                                 aGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTgg                                                                                                <  3:963849/149‑1 (MQ=255)
                                                  gATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTggg                                                                                               <  3:356716/149‑1 (MQ=255)
                                                  gATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTggg                                                                                               >  2:466067/1‑149 (MQ=255)
                                                    ttATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTa                                                                                             >  2:585024/1‑149 (MQ=255)
                                                     tATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTaa                                                                                            <  1:475242/149‑1 (MQ=255)
                                                       tCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAAtt                                                                                          <  2:504841/149‑1 (MQ=255)
                                                         cTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCt                                                                                        >  4:329836/1‑149 (MQ=255)
                                                           aaaGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTa                                                                                      <  3:86965/149‑1 (MQ=255)
                                                              gTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAg                                                                                   <  2:450394/149‑1 (MQ=255)
                                                                   acacaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTAtt                                                                              >  1:1079770/1‑149 (MQ=255)
                                                                   acacaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTAtt                                                                              >  2:627895/1‑149 (MQ=255)
                                                                     acaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATc                                                                                                           >  4:315156/1‑118 (MQ=255)
                                                                     acaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTc                                                                             >  2:478154/1‑148 (MQ=255)
                                                                       aTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCAc                                                                          <  2:325189/149‑1 (MQ=255)
                                                                       aTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCAc                                                                          >  4:182290/1‑149 (MQ=255)
                                                                       aTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCAc                                                                          >  3:796384/1‑149 (MQ=255)
                                                                       aTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCAc                                                                          >  3:137761/1‑149 (MQ=255)
                                                                        tttGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCAcc                                                                         >  2:660061/1‑149 (MQ=255)
                                                                        tttGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCAcc                                                                         >  3:304871/1‑149 (MQ=255)
                                                                        tttGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCAcc                                                                         >  3:1080470/1‑149 (MQ=255)
                                                                            cTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTaa                                                                     >  3:190710/1‑149 (MQ=255)
                                                                             tgtgTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAAt                                                                    >  1:530313/1‑149 (MQ=255)
                                                                               tgtAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTa                                                                  >  2:189386/1‑149 (MQ=255)
                                                                               tgcaaaaCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAAGTa                                                                  >  3:718551/4‑149 (MQ=255)
                                                                                 tAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACt                                                                <  4:137761/149‑1 (MQ=255)
                                                                                  aaaaCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACtt                                                               >  3:659186/1‑149 (MQ=255)
                                                                                  aaaaCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACtt                                                               >  2:7633/1‑149 (MQ=255)
                                                                                      cGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCaaa                                                           <  1:466067/149‑1 (MQ=255)
                                                                                       gAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAAt                                                          <  1:973956/149‑1 (MQ=255)
                                                                                       gAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAAt                                                          >  1:156672/1‑149 (MQ=255)
                                                                                         ggggTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTc                                                        >  1:1045773/1‑149 (MQ=255)
                                                                                           ggTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTa                                                      >  1:280775/1‑149 (MQ=255)
                                                                                                           gagaGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGt                                                                                              <  2:979312/93‑1 (MQ=255)
                                                                                                           gagaGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGt                                                                                              >  1:979312/1‑93 (MQ=255)
                                                                                                                 aaaaTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtgat                                <  4:518826/149‑1 (MQ=255)
                                                                                                                      gCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTgg                                                                                                <  2:8661/80‑1 (MQ=255)
                                                                                                                       ccACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACcac                          >  2:252083/1‑149 (MQ=255)
                                                                                                                       ccACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACcac                          <  4:875455/149‑1 (MQ=255)
                                                                                                                        cACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGt                                                                                 >  4:806084/1‑93 (MQ=255)
                                                                                                                        cACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGt                                                                                 <  3:806084/93‑1 (MQ=255)
                                                                                                                        cACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCaaa                                                           >  1:1080350/1‑115 (MQ=255)
                                                                                                                           gCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCacaaca                      <  1:189386/149‑1 (MQ=255)
                                                                                                                             tctGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAAcatc                    >  2:557829/1‑149 (MQ=255)
                                                                                                                              ctGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAAcatca                   <  3:234128/149‑1 (MQ=255)
                                                                                                                                gTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACatcatc                 <  1:660061/149‑1 (MQ=255)
                                                                                                                                   cGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttc              <  1:969541/149‑1 (MQ=255)
                                                                                                                                    gTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttct             >  4:426350/1‑149 (MQ=255)
                                                                                                                                     ttGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttctt            >  3:1006280/1‑149 (MQ=255)
                                                                                                                                      aggaaaaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttcttc           >  4:809473/5‑149 (MQ=255)
                                                                                                                                       ggaaaaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttcttct          >  4:326829/4‑149 (MQ=255)
                                                                                                                                       gATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttcttct          >  4:888843/1‑149 (MQ=255)
                                                                                                                                       gATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttcttct          >  3:1043951/1‑149 (MQ=255)
                                                                                                                                        gaaaaaGGCCGCTAAGCGGCCTTATTACTTTCTTTACAATACTTCAACATGTTGAGTATTGGGTAATTCTTACAGGTATTCGACCTTAATTACTTAATATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATcttcttctt         >  2:247434/3‑149 (MQ=255)
                                                                                                                                        gaaaaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttcttc           <  4:7530/145‑1 (MQ=255)
                                                                                                                                         aaaaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGtttt                                   >  2:959179/2‑122 (MQ=255)
                                                                                                                                         aaaaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGtttt                                   <  1:959179/121‑1 (MQ=255)
                                                                                                                                         aaaaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCttt        <  3:326829/148‑1 (MQ=255)
                                                                                                                                          aaaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTAc                                                                 >  1:464432/1‑91 (MQ=255)
                                                                                                                                          aaaaGGCCGCATAGCGGCCTTTTCCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCACCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTg       >  4:105204/1‑149 (MQ=255)
                                                                                                                                           aaaGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGc      <  1:180057/149‑1 (MQ=255)
                                                                                                                                           aaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCaaa                                                           >  3:321139/1‑96 (MQ=255)
                                                                                                                                               gCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAt  <  4:48266/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAT  >  NC_000913/3328034‑3328325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: