Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,458,556 C→A 61.9% S73* (TCG→TAG)  gspE → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,458,5560CA61.9% 90.0 / 163.3 118S73* (TCG→TAG) gspEgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (24/21);  new base A (32/41);  total (56/62)
Fisher's exact test for biased strand distribution p-value = 3.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

TTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAA  >  NC_000913/3458418‑3458704
                                                                                                                                          |                                                                                                                                                    
  tatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTgc                                                                                                                                          >  1:365080/1‑149 (MQ=255)
    tctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTgcgc                                                                                                                                        >  4:224521/1‑149 (MQ=255)
    tctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTgcgc                                                                                                                                        >  1:488116/1‑149 (MQ=255)
     ctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCa                                                                                                                                       <  3:463502/149‑1 (MQ=255)
     ctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCa                                                                                                                                       <  4:262476/149‑1 (MQ=255)
      tcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAg                                                                                                                                      >  3:253989/1‑149 (MQ=255)
      tcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAg                                                                                                                                      <  2:397863/149‑1 (MQ=255)
        gCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGa                                                                                                                                    >  1:461053/1‑149 (MQ=255)
            aTACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTgcgc                                                                                                                                        <  2:644752/141‑1 (MQ=255)
            aTACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTgcgc                                                                                                                                        >  1:644752/1‑141 (MQ=255)
              aCGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGa                                                                                                                              <  2:365080/149‑1 (MQ=255)
               cGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGAt                                                                                                                             <  4:253989/149‑1 (MQ=255)
               cGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGAt                                                                                                                             <  1:246542/149‑1 (MQ=255)
               cGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGAt                                                                                                                             <  1:970418/149‑1 (MQ=255)
                   aTTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAAt                                                                                                                         >  4:165892/1‑149 (MQ=255)
                    ttCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATc                                                                                                                        >  3:474891/1‑149 (MQ=255)
                    ttCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATc                                                                                                                        >  4:639853/1‑149 (MQ=255)
                      cGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCg                                                                                                                      <  2:31189/149‑1 (MQ=255)
                       gAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGTCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGt                                                                                                                     <  3:475823/149‑1 (MQ=255)
                       gAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGt                                                                                                                     <  3:165892/149‑1 (MQ=255)
                       gAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGAt                                                                                                                             >  1:360038/1‑141 (MQ=255)
                       gAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGAt                                                                                                                             <  2:360038/141‑1 (MQ=255)
                       gAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGt                                                                                                                     >  2:714382/1‑149 (MQ=255)
                       gAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGt                                                                                                                     <  1:150491/149‑1 (MQ=255)
                       gAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGt                                                                                                                     >  3:960449/1‑149 (MQ=255)
                            gTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGAtc                                                                                                                >  2:994887/1‑149 (MQ=255)
                                cTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGAtcttct                                                                                                            >  1:225301/1‑149 (MQ=255)
                                 tCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTtctc                                                                                                           >  2:706344/1‑149 (MQ=255)
                                   gATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTtctctc                                                                                                         >  2:959682/1‑149 (MQ=255)
                                   gATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTtctctc                                                                                                         >  4:706931/1‑149 (MQ=255)
                                   gATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTtctctc                                                                                                         <  1:301484/149‑1 (MQ=255)
                                   gATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTtctc                                                                                                           <  4:901049/147‑1 (MQ=255)
                                   gATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTtctc                                                                                                           >  3:901049/1‑147 (MQ=255)
                                         cAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTc                                                                                                   >  3:311390/1‑149 (MQ=255)
                                          aaCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCg                                                                                                  >  1:642314/1‑149 (MQ=255)
                                           aCGTCAGGTTGGGCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCgg                                                                                                 <  1:752457/149‑1 (MQ=255)
                                           aCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCgg                                                                                                 >  3:92186/1‑149 (MQ=255)
                                              tCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAag                                                                                              <  1:377818/149‑1 (MQ=255)
                                                  gTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGAt                                                                                          <  1:722146/149‑1 (MQ=255)
                                                        cAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGt                                                                                                                     >  3:594492/1‑116 (MQ=255)
                                                        cAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGt                                                                                                                     <  4:594492/116‑1 (MQ=255)
                                                               aGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGaa                                                                             <  4:581607/149‑1 (MQ=255)
                                                               aGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGaa                                                                             >  4:723527/1‑149 (MQ=255)
                                                                   cATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGa                                                                              <  1:989721/144‑1 (MQ=255)
                                                                   cATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGa                                                                              >  2:989721/1‑144 (MQ=255)
                                                                   cATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGAtc                                                                         >  3:174837/1‑149 (MQ=255)
                                                                      gACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTcc                                                                      <  1:572296/149‑1 (MQ=255)
                                                                      gACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTcc                                                                      <  3:269559/149‑1 (MQ=255)
                                                                      gACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTcc                                                                      <  4:315830/149‑1 (MQ=255)
                                                                      gACCAGCTTGTCACAGGCTGATTTTGAAGCGCGGCTGGAAGTGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTcc                                                                      <  2:858198/149‑1 (MQ=255)
                                                                       aCCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCt                                                                     <  3:585399/149‑1 (MQ=255)
                                                                          aGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCtgaa                                                                  <  2:523813/149‑1 (MQ=255)
                                                                           gCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGa                                                                                                                              >  4:196152/1‑88 (MQ=255)
                                                                           gCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGa                                                                                                                              <  3:196152/88‑1 (MQ=255)
                                                                           gCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCtgaat                                                                 <  3:643327/149‑1 (MQ=255)
                                                                              tGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCtgaatgaa                                                              <  2:916266/149‑1 (MQ=255)
                                                                              tGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCtgaatgaa                                                              <  2:151408/149‑1 (MQ=255)
                                                                                tCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGa                                                            <  4:174837/149‑1 (MQ=255)
                                                                                tCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGa                                                            >  3:155864/1‑149 (MQ=255)
                                                                                tCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGa                                                            <  3:520831/149‑1 (MQ=255)
                                                                                tCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGa                                                            >  1:911008/1‑149 (MQ=255)
                                                                                  acaGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAgaga                                                                                           >  2:758403/1‑116 (MQ=255)
                                                                                  acaGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGAtt                                                          >  2:464471/1‑149 (MQ=255)
                                                                                  acaGGCTGAATTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCTGTCCATCTTCTCTCGCTTTCGGAAGAGGTCCCCGCAAATGAAGATCTCGTGAATGAAGAtt                                                          >  2:851554/1‑149 (MQ=255)
                                                                                   caGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTc                                                         <  1:1062935/149‑1 (MQ=255)
                                                                                    aGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGGTGCCCGCaaa                                                                                 <  1:634029/124‑1 (MQ=255)
                                                                                    aGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCaaa                                                                                 >  2:634029/1‑124 (MQ=255)
                                                                                      gCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAgaga                                                                                           >  2:259275/1‑112 (MQ=255)
                                                                                            tttGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCAcc                                                >  3:555623/1‑149 (MQ=255)
                                                                                             ttGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGATGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCg                                               <  1:714382/149‑1 (MQ=255)
                                                                                                 aGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTa                                           >  2:331857/1‑149 (MQ=255)
                                                                                                   cTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATc                                         <  3:1062812/149‑1 (MQ=255)
                                                                                                      ggCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGc                                      <  1:959682/149‑1 (MQ=255)
                                                                                                        cTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCtt                                    <  3:384076/149‑1 (MQ=255)
                                                                                                        cTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCtt                                    <  3:533081/149‑1 (MQ=255)
                                                                                                        cTGGAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCtt                                    <  4:92186/149‑1 (MQ=255)
                                                                                                          ggAAGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGa                                  <  2:189159/149‑1 (MQ=255)
                                                                                                             aGCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGACCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCa                               >  1:150321/1‑149 (MQ=255)
                                                                                                              gCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCaa                              <  2:488116/149‑1 (MQ=255)
                                                                                                              gCGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCaa                              >  2:38857/1‑149 (MQ=255)
                                                                                                               cGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAAt                             <  4:960449/149‑1 (MQ=255)
                                                                                                               cGGTATTCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAAt                             <  1:126883/149‑1 (MQ=255)
                                                                                                                    ttCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGAt                        >  3:139626/1‑149 (MQ=255)
                                                                                                                    ttCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGAt                        >  1:947482/1‑149 (MQ=255)
                                                                                                                    ttCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGAt                        >  1:460334/1‑149 (MQ=255)
                                                                                                                    ttCCATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGAt                        >  4:132370/1‑149 (MQ=255)
                                                                                                                       cATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGTACCGGTTATCCGCTTGATCAATGCGAtttt                     <  1:535068/149‑1 (MQ=255)
                                                                                                                       cATCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAg                                                             <  3:1035636/109‑1 (MQ=255)
                                                                                                                        aTCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCt                                                                     <  3:298836/100‑1 (MQ=255)
                                                                                                                        aTCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCt                                                                     >  4:298836/1‑100 (MQ=255)
                                                                                                                        aTCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCt                                     <  3:971004/132‑1 (MQ=255)
                                                                                                                        aTCAGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTtg                    >  4:902564/1‑149 (MQ=255)
                                                                                                                         tCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGGTTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTtga                   <  2:761063/149‑1 (MQ=255)
                                                                                                                         tCAAAACACCGGTGAGTAGAAACGGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCCCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTtga                   <  4:500970/149‑1 (MQ=255)
                                                                                                                           aGAATACCGGTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTtgagt                 <  4:311390/149‑1 (MQ=255)
                                                                                                                            gAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTtgagtg                >  3:302045/1‑149 (MQ=255)
                                                                                                                                   ggTGAGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCAt         <  1:357309/149‑1 (MQ=255)
                                                                                                                                       aGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCaaa     <  2:999518/149‑1 (MQ=255)
                                                                                                                                       aGTAGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCaaa     <  4:182242/149‑1 (MQ=255)
                                                                                                                                          cGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGaa  <  3:983958/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
TTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAA  >  NC_000913/3458418‑3458704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: