Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,522,779 T→A 6.0% intergenic (‑28/‑92) hofM ← / → mrcA DNA catabolic putative pilus assembly protein/penicillin‑binding protein 1a, murein transglycosylase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,522,7790TA 6.0% 464.2 / 14.1 135intergenic (‑28/‑92)hofM/mrcADNA catabolic putative pilus assembly protein/penicillin‑binding protein 1a, murein transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base T (60/66);  new base A (3/5);  total (63/72)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 138 total aligned reads displayed.

CAGCGGCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGGGAGCA  >  NC_000913/3522635‑3522927
                                                                                                                                                |                                                                                                                                                    
 agtggCAACCGCCACCTGCGTTGCAAATAGCATTCTTTTCCGCCCCGCACGATCGCAACCGTTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGTCCGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAATCTGAc                                                                                                                                                 <  2:398334/146‑1 (MQ=255)
    ggCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACggg                                                                                                                                              <  1:181134/149‑1 (MQ=255)
     gCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAGAGTGACGGGt                                                                                                                                             <  2:482688/149‑1 (MQ=255)
     gCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGt                                                                                                                                             <  4:532254/149‑1 (MQ=255)
      cAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAGAGTGACGGGtg                                                                                                                                            <  1:870283/149‑2 (MQ=255)
      cAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATAATCTCCTTATCACCCGTCACTCTGACGGGta                                                                                                                                            <  4:163632/149‑1 (MQ=255)
               ccaGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGc                                                                                                                                   <  4:847896/149‑1 (MQ=255)
                caGCGTTGCACAAAGCATTCTTTTAGGCCCCGCACGATCGCAACCGGTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCg                                                                                                                                  >  4:511243/1‑149 (MQ=255)
                    gTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTg                                                                                                                              >  1:1068331/1‑149 (MQ=255)
                        cAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACgg                                                                                                                                               >  3:288670/1‑128 (MQ=255)
                        cAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCtt                                                                                                                          <  4:1071643/149‑1 (MQ=255)
                         aaaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTg                                                                                                                         >  4:636865/1‑149 (MQ=255)
                         aaaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTg                                                                                                                         >  4:401978/1‑149 (MQ=255)
                         aaaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTg                                                                                                                         <  2:311149/149‑1 (MQ=255)
                         aaaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTg                                                                                                                         >  3:752835/1‑149 (MQ=255)
                         aaaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTg                                                                                                                         >  3:939486/1‑149 (MQ=255)
                          aaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGc                                                                                                                        <  1:991916/149‑1 (MQ=255)
                          aaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGc                                                                                                                        >  1:415868/1‑149 (MQ=255)
                          aaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGc                                                                                                                        <  4:405225/149‑1 (MQ=255)
                            aaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCt                                                                                                                      >  3:1071314/1‑149 (MQ=255)
                               cATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTta                                                                                                                   <  2:53492/149‑1 (MQ=255)
                               cATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTta                                                                                                                   <  4:1045150/149‑1 (MQ=255)
                                aTTCTATTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTtat                                                                                                                  <  2:1068331/149‑1 (MQ=255)
                                 ttCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTtata                                                                                                                 >  2:308660/1‑149 (MQ=255)
                                  tCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTAtac                                                                                                                >  3:33303/1‑149 (MQ=255)
                                  tCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTAtac                                                                                                                <  3:586340/149‑1 (MQ=255)
                                    ttttGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTAtacta                                                                                                              >  1:1035214/1‑149 (MQ=255)
                                    ttttGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTAtacta                                                                                                              >  1:431863/1‑149 (MQ=255)
                                          gcCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGc                                                                                                                            >  3:151683/1‑129 (MQ=255)
                                          gcCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGc                                                                                                                            <  4:151683/129‑1 (MQ=255)
                                          gcCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACcgcgc                                                                                                        <  1:626014/149‑1 (MQ=255)
                                              cGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgttt                                                                                                    <  1:627082/149‑1 (MQ=255)
                                                     tCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTtata                                                                                             >  1:587331/1‑149 (MQ=255)
                                                       gCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATaaa                                                                                           <  3:273990/149‑1 (MQ=255)
                                                       gCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATaaa                                                                                           >  3:71165/1‑149 (MQ=255)
                                                         aaCCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACt                                                                                         <  3:401978/149‑1 (MQ=255)
                                                             gctaccgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAGAGTGACGGGtg                                                                                                                                            <  2:510102/94‑2 (MQ=255)
                                                             gctaccgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAGAGTGACGGGtg                                                                                                                                            >  1:510102/1‑93 (MQ=255)
                                                                accgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCaaa                                                                                  <  2:733255/149‑1 (MQ=255)
                                                                accgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCaaa                                                                                  <  1:762127/149‑1 (MQ=255)
                                                                 ccgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAAt                                                                                 <  2:886884/149‑1 (MQ=255)
                                                                     ttcttGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGaaa                                                                             >  1:147682/1‑149 (MQ=255)
                                                                         tGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTaa                                                                         <  1:953496/149‑1 (MQ=255)
                                                                           cTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAAt                                                                       <  1:3219/149‑1 (MQ=255)
                                                                           cTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAAt                                                                       <  1:621349/149‑1 (MQ=255)
                                                                            tgttgtAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATg                                                                      >  1:506653/1‑149 (MQ=255)
                                                                              ttgtAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATggg                                                                    >  1:350247/1‑149 (MQ=255)
                                                                               tgtAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGa                                                                   <  1:553224/149‑1 (MQ=255)
                                                                                 tAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGaaa                                                                 >  3:340963/1‑149 (MQ=255)
                                                                                 tAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGaaa                                                                 >  2:20192/1‑149 (MQ=255)
                                                                                     tGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTc                                                             >  2:1055386/1‑149 (MQ=255)
                                                                                       cAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCa                                                           <  2:800973/149‑1 (MQ=255)
                                                                                        aaaCCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTtat                                                                                                                  <  1:77331/93‑1 (MQ=255)
                                                                                        aaaCCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTtat                                                                                                                  >  2:77331/1‑93 (MQ=255)
                                                                                         aaCCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCaaa                                                                                  >  2:996898/1‑124 (MQ=255)
                                                                                         aaCCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGt                                                         >  3:45966/1‑149 (MQ=255)
                                                                                          actaaTTTGCCTGATCTTAAACGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTta                                                                                                                   >  4:341660/4‑90 (MQ=255)
                                                                                          aCCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTta                                                                                                                   <  3:341660/90‑1 (MQ=255)
                                                                                                ttGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTc                                                  >  1:733101/1‑149 (MQ=255)
                                                                                                   ccAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTa                                               >  3:541691/1‑149 (MQ=255)
                                                                                                    cAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATgg                                                                     <  1:311116/126‑1 (MQ=255)
                                                                                                     aGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTaaa                                             <  3:118029/149‑1 (MQ=255)
                                                                                                     aGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTaaa                                             >  4:176409/1‑149 (MQ=255)
                                                                                                     aGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTaaa                                             >  2:665726/1‑149 (MQ=255)
                                                                                                     aGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACGAAATGGGAAATTTCCAGTGAAGTTCGTaaa                                             >  1:714880/1‑149 (MQ=255)
                                                                                                        tCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAGAGTGACGGGtg                                                                                                                                            <  4:930303/51‑2 (MQ=25)
                                                                                                        tCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAGAGTGACGGGtg                                                                                                                                            >  3:930303/1‑50 (MQ=25)
                                                                                                           tAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTta                                                                                               <  4:534324/93‑1 (MQ=255)
                                                                                                           tAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTcc                                                            <  1:869956/128‑1 (MQ=255)
                                                                                                           tAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAttt                                       <  2:714880/149‑1 (MQ=255)
                                                                                                             aaTGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAttttt                                     <  1:915494/149‑1 (MQ=255)
                                                                                                             aaTGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAttttt                                     >  1:1046409/1‑149 (MQ=255)
                                                                                                              aTGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAAt                                                                                 <  3:135367/104‑1 (MQ=255)
                                                                                                                tccATTGTGATGATCTCCCTATCACCCCTCACCCTGACCGGTATATCAAAGGGTGTGGCTTTCCTTTAAACCACCGCGCCTTTGTTTAAAAAATGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGa                                  <  1:464175/148‑1 (MQ=255)
                                                                                                                gCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCaaa                                                                                  <  3:133125/101‑1 (MQ=255)
                                                                                                                gCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCaaa                                                                                  >  4:133125/1‑101 (MQ=255)
                                                                                                                gCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGa                                  <  2:1032711/149‑1 (MQ=255)
                                                                                                                gCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGa                                  <  1:243488/149‑1 (MQ=255)
                                                                                                                   aTTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATcc                               >  3:1053193/1‑149 (MQ=255)
                                                                                                                     tgtgATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCtt                             <  3:284482/149‑1 (MQ=255)
                                                                                                                       tgatgaTCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGc                           >  4:835157/1‑149 (MQ=255)
                                                                                                                         atgatCTACTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAACCTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAg                         >  4:679943/1‑149 (MQ=255)
                                                                                                                               tcCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGtt                   <  1:712460/149‑1 (MQ=255)
                                                                                                                               tcCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGtt                   >  2:207578/1‑149 (MQ=255)
                                                                                                                               tcCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGtt                   >  4:371027/1‑149 (MQ=255)
                                                                                                                               tcCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGtt                   <  2:506653/149‑1 (MQ=255)
                                                                                                                               tcCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGtt                   <  2:51678/149‑1 (MQ=255)
                                                                                                                                ccTTATCACCCTTCTCTTTGACGGGTATATCAATGCGTCTGGCTTGTCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAATCTAAATGGGGAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTg                  <  3:62060/149‑1 (MQ=255)
                                                                                                                                 cTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCa                          >  3:367854/1‑140 (MQ=255)
                                                                                                                                  ttATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGtt                   >  4:132073/1‑146 (MQ=255)
                                                                                                                                  ttATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGtt                   <  3:132073/146‑1 (MQ=255)
                                                                                                                                    aTCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCAtt              <  3:176409/149‑1 (MQ=255)
                                                                                                                                     tCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTc             <  4:340669/149‑1 (MQ=255)
                                                                                                                                     tCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTc             <  3:380191/149‑1 (MQ=255)
                                                                                                                                         ccGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTctgct         >  3:451028/1‑149 (MQ=255)
                                                                                                                                           gtaaatctGACGGGAATATCAATGCGTCTGGCTGGCCTTGATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTGCAGTGAAGGTCGTAAAGTGTTTTTTGAACCTTGCAATCTGTTGCATTCTGCTgg       >  4:1014666/6‑149 (MQ=255)
                                                                                                                                             cACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGGGa     <  2:587331/149‑1 (MQ=255)
                                                                                                                                             cACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGGGa     <  3:894998/149‑1 (MQ=255)
                                                                                                                                             cACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGGGa     <  4:939486/149‑1 (MQ=255)
                                                                                                                                                tctGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGGGAGCa  <  4:752835/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CAGCGGCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGGGAGCA  >  NC_000913/3522635‑3522927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: