Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,601,824 G→C 5.1% P88R (CCC→CGC)  ftsX ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,601,8240GC 5.1% 466.3 / 10.0 134P88R (CCC→CGC) ftsXputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (62/65);  new base C (4/3);  total (66/68)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

CACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGA  >  NC_000913/3601676‑3601969
                                                                                                                                                    |                                                                                                                                                 
cACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTgg                                                                                                                                                   >  2:336642/1‑149 (MQ=255)
cACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTgg                                                                                                                                                   <  4:1070866/149‑1 (MQ=255)
 aCGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTggg                                                                                                                                                  >  2:802773/1‑149 (MQ=255)
   gCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCa                                                                                                                                                >  4:30652/1‑149 (MQ=255)
      ttGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCg                                                                                                                                             >  4:418721/1‑149 (MQ=255)
      ttGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCg                                                                                                                                             >  1:675719/1‑149 (MQ=255)
         cTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTct                                                                                                                                          <  3:285032/149‑2 (MQ=255)
         cTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTct                                                                                                                                          <  3:586111/149‑2 (MQ=255)
             gCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAgaga                                                                                                                                      >  4:308683/1‑149 (MQ=255)
              ccTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGTAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGaa                                                                                                                                     >  1:232485/1‑149 (MQ=255)
               cTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGaaa                                                                                                                                    <  3:406378/149‑1 (MQ=255)
                tGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAAt                                                                                                                                   <  3:402483/149‑1 (MQ=255)
                 gCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTctgtt                                                                                                                                       <  1:692035/144‑5 (MQ=255)
                 gCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTctgtt                                                                                                                                       >  2:692035/1‑140 (MQ=255)
                  cAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATgg                                                                                                                                 >  1:423742/1‑149 (MQ=255)
                   aaCTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGc                                                                                                                                <  1:194550/149‑1 (MQ=255)
                   aaCTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGc                                                                                                                                >  1:1058550/1‑149 (MQ=255)
                    aCTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCg                                                                                                                               >  4:1068919/1‑149 (MQ=255)
                    aCTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCg                                                                                                                               >  3:851742/1‑149 (MQ=255)
                      tgtgCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGAt                                                                                                                             >  4:241444/1‑149 (MQ=255)
                        tgCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATaa                                                                                                                           <  2:995124/149‑1 (MQ=255)
                        tgCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATaa                                                                                                                           <  3:1053364/149‑1 (MQ=255)
                         gCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAAc                                                                                                                          >  3:253256/1‑149 (MQ=255)
                           caccacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCa                                                                                                                        >  3:234340/1‑149 (MQ=255)
                            accacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCAt                                                                                                                       >  2:167124/1‑149 (MQ=255)
                             ccacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATc                                                                                                                      >  2:754551/1‑149 (MQ=255)
                                   ccGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGtt                                                                                                                >  1:542656/1‑149 (MQ=255)
                                   ccGCAGCAGCGTCATCGTCCAGCGTTTTGTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGtt                                                                                                                >  3:927788/1‑149 (MQ=255)
                                     gcagcaGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTaa                                                                                                              >  1:884220/1‑149 (MQ=255)
                                        gcagcGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAATGTTTTTGTACACCATAAAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTaaaaa                                                                                                           >  4:822176/1‑149 (MQ=255)
                                          agcGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTgg                                                                                                                                                   <  3:103990/107‑1 (MQ=255)
                                          agcGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTgg                                                                                                                                                   >  4:103990/1‑107 (MQ=255)
                                           gcGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGt                                                                                                        <  4:619373/149‑1 (MQ=255)
                                           gcGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTCATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACAACATATAACAGACGCTCGGCAGCGTCAAAGACATGGCGATAACCATCACCGTTAAAAACGt                                                                                                        <  4:818361/149‑1 (MQ=255)
                                            cGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTg                                                                                                       <  3:808390/149‑1 (MQ=255)
                                            cGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTg                                                                                                       <  4:640108/149‑1 (MQ=255)
                                             gTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTgg                                                                                                      <  4:452871/149‑1 (MQ=255)
                                                aTCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGc                                                                                                                                >  3:30378/1‑120 (MQ=255)
                                                aTCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGa                                                                                                                              <  4:57578/122‑1 (MQ=255)
                                                aTCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGa                                                                                                                              >  3:57578/1‑122 (MQ=255)
                                                   gTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAAct                                                                                                <  2:935036/149‑2 (MQ=255)
                                                    tCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACgg                                                                                               >  4:781173/1‑149 (MQ=255)
                                                        gCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTg                                                                                           >  3:689405/1‑149 (MQ=255)
                                                                  cAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGAt                                                                                 <  3:1068919/149‑1 (MQ=255)
                                                                  cAGATAAACAGTGATTTCCGGTGACGGATAATACTGCGTCGCCGCCTGGCTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCTCCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGAt                                                                                 <  4:912109/149‑1 (MQ=255)
                                                                   aGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATc                                                                                >  2:143504/1‑149 (MQ=255)
                                                                   aGATAAACAGTGATTTGCGGAAACGGAGAATACTGCGTCGCCGCCTGGTTAACGTTTTGGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAACGGCGATAAGCAGCACAGCTAAAAACGTGGCGAACGGTTTGCTTTTCAGATc                                                                                >  2:275898/1‑149 (MQ=255)
                                                                     aTAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCt                                                                              <  4:437840/149‑1 (MQ=255)
                                                                       aaaCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGc                                                                            <  2:318696/149‑1 (MQ=255)
                                                                         aCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCt                                                                              >  4:283287/1‑145 (MQ=255)
                                                                                     ggTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGaa                                                              <  3:945380/149‑1 (MQ=255)
                                                                                         aCGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCa                                                          >  1:966647/1‑149 (MQ=255)
                                                                                                      cGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGtt                                             <  3:825861/149‑1 (MQ=255)
                                                                                                       gTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTc                                            <  2:857661/149‑1 (MQ=255)
                                                                                                         cgccgcCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGt                                          >  1:112516/1‑149 (MQ=255)
                                                                                                          gccgccTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGtt                                         <  2:937899/149‑1 (MQ=255)
                                                                                                          gccgccTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGCTCGtt                                         <  4:851742/149‑1 (MQ=255)
                                                                                                           ccgccTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTg                                        <  3:454242/149‑1 (MQ=255)
                                                                                                               cTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGaaaa                                    >  2:827934/1‑149 (MQ=255)
                                                                                                               cTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGaaaa                                    >  4:467823/1‑149 (MQ=255)
                                                                                                                tGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAAc                                   >  2:978454/1‑149 (MQ=255)
                                                                                                                  gTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGt                                 >  4:394156/1‑149 (MQ=255)
                                                                                                                  attAACGTGGTTGTACACCACATAACAGACGCTGGGCAGCGTTAGAGAAATGGCGATAACCATCACCGTTAAAAACGCGGCGAACGGTTTGATTTCCAGATCCTGCAGTGCGCCTTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGt                                 >  4:935965/2‑149 (MQ=255)
                                                                                                                   ttAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGtt                                <  1:811204/149‑1 (MQ=255)
                                                                                                                    tAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTg                               <  1:1022266/149‑1 (MQ=255)
                                                                                                                     aaCGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCtt                                                                                        <  1:64305/91‑1 (MQ=255)
                                                                                                                     aaCGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCtt                                                                                        >  2:64305/1‑91 (MQ=255)
                                                                                                                        gTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCAt                                                         >  3:223788/1‑119 (MQ=255)
                                                                                                                        gTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCAt                                                         <  4:223788/119‑1 (MQ=255)
                                                                                                                         tttttGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGtttt                          <  4:460872/149‑1 (MQ=255)
                                                                                                                          ttttGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCa                                                                                                                     >  1:959921/1‑57 (MQ=255)
                                                                                                                          ttttGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTg                         <  1:793143/149‑1 (MQ=255)
                                                                                                                            ttGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCg                       <  1:802773/149‑1 (MQ=255)
                                                                                                                              gTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGAt                     >  4:932840/1‑149 (MQ=255)
                                                                                                                              ctACACCATATAACATCCCGCACGCAGCGTGCGACAAGTGGCGATAACCATCAACGGTAAAAAAGTGGGGGGCGGTTTGCTTTTCAGATCCTTCAATGCGCCGTGGAAGGCATCGCGCACCTGTTAGCTGAAAACGTAGATTTTGCGAt                     >  4:857372/2‑149 (MQ=255)
                                                                                                                                  acCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTAc                 >  1:479910/1‑149 (MQ=255)
                                                                                                                                   ccATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTAcc                >  1:26926/1‑149 (MQ=255)
                                                                                                                                     atatAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAGACGTTGGTTTTGCGATTTACCgg              >  2:361523/1‑149 (MQ=255)
                                                                                                                                      tataACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGt             <  2:1058550/149‑1 (MQ=255)
                                                                                                                                        taACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGtt                                         >  2:735113/1‑119 (MQ=255)
                                                                                                                                        taACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGtt                                         <  1:735113/119‑1 (MQ=255)
                                                                                                                                        taACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGttt           >  3:447630/1‑149 (MQ=255)
                                                                                                                                         aaCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGtttt          <  2:1092380/149‑1 (MQ=255)
                                                                                                                                         aaCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGtttt          <  3:344111/149‑1 (MQ=255)
                                                                                                                                          tctgACGCTGCCCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTg         >  3:450627/4‑149 (MQ=255)
                                                                                                                                          tctgACGCTGCCCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTg         >  2:68500/4‑149 (MQ=255)
                                                                                                                                          aCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTg         <  2:232485/149‑1 (MQ=255)
                                                                                                                                          aCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTg         <  3:781173/149‑1 (MQ=255)
                                                                                                                                          aCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTg         >  2:961031/1‑149 (MQ=255)
                                                                                                                                           cAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTgg        <  1:868187/149‑1 (MQ=255)
                                                                                                                                            aGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGc       <  2:508769/149‑1 (MQ=255)
                                                                                                                                            aGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGCTTTGGc       >  2:998845/1‑149 (MQ=255)
                                                                                                                                             gACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCg      >  2:1076698/1‑149 (MQ=255)
                                                                                                                                              aCGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGa     <  2:806292/149‑1 (MQ=255)
                                                                                                                                               cGCTGGGCAGCGTCAGAGAAATGGCGATAGCCATCACCGTTAAAAACGTGGCGAACGGTCTGCTTTTAAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAACACGTTGGTTTTGCGATTTACCGGTTTTAGCGAg    <  2:287914/149‑1 (MQ=255)
                                                                                                                                                gCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCGCCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAgg   <  2:479910/149‑1 (MQ=255)
                                                                                                                                                gCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTgg        <  2:941204/144‑1 (MQ=255)
                                                                                                                                                gCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTgg        >  1:941204/1‑144 (MQ=255)
                                                                                                                                                 cTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGa  <  2:62647/149‑1 (MQ=255)
                                                                                                                                                 cTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGa  <  4:608592/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGA  >  NC_000913/3601676‑3601969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: