Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,697,969 C→G 5.8% H504Q (CAC→CAG bcsE → cellulose production protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,697,9690CG 5.8% 351.7 / 9.2 101H504Q (CAC→CAGbcsEcellulose production protein
Reads supporting (aligned to +/- strand):  ref base C (42/53);  new base G (2/4);  total (44/57)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 101 total aligned reads displayed.

CAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGGCGCGGCACTCTTTGCGA  >  NC_000913/3697824‑3698115
                                                                                                                                                 |                                                                                                                                                  
cAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGTTCATCAATGCCGAGCACGATGGTCGCCACTgg                                                                                                                                                 <  2:744880/149‑1 (MQ=255)
  aaCCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcg                                                                                                                                               >  1:810761/1‑149 (MQ=255)
  aaCCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcg                                                                                                                                               <  1:501463/149‑1 (MQ=255)
      gTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCAGTGGcgacca                                                                                                                                           <  4:821762/149‑3 (MQ=255)
      gTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCAGTGGcgacca                                                                                                                                           <  3:728102/149‑3 (MQ=255)
        aTGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCAGTGGcgaccatc                                                                                                                                         <  2:731922/149‑5 (MQ=255)
        aTGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGGGACGAAt                                                                                                                                         <  2:602457/149‑1 (MQ=255)
         tGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATa                                                                                                                                        >  4:833580/1‑149 (MQ=255)
                   tGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCa                                                                                                                              <  1:970982/149‑1 (MQ=255)
                    gAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCAGTGGcgaccatc                                                                                                                                         <  1:1059698/137‑5 (MQ=255)
                    gAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCAGTGGcgaccatc                                                                                                                                         >  2:1059698/1‑133 (MQ=255)
                    gAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCAt                                                                                                                             <  2:364148/149‑1 (MQ=255)
                      aGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGc                                                                                                                           <  1:165420/149‑1 (MQ=255)
                       gatgatCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCg                                                                                                                          >  4:1007266/1‑149 (MQ=255)
                       gatgatCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCg                                                                                                                          >  4:966665/1‑149 (MQ=255)
                        atgatCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGa                                                                                                                         >  1:647961/1‑149 (MQ=255)
                          gatCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcgac                                                                                                                                             >  1:122380/1‑127 (MQ=255)
                          gatCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcgac                                                                                                                                             <  2:122380/127‑1 (MQ=255)
                          gatCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACt                                                                                                                       <  4:582243/149‑1 (MQ=255)
                            tCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGt                                                                                                                     >  4:481165/1‑149 (MQ=255)
                            tCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGt                                                                                                                     >  3:1023356/1‑149 (MQ=255)
                             cAAATCAGTGCCGAGCTTGTGCACATGCGCTTGCTTGCCCCCGAACAATGGGGCATGCCGATGCCTTTAACGCAAAGTTCTAAACCGGTAATCAATGCCGAGCACGATGGTCGCCACTGGGGACGAATACCAGAACCCATGCGACTGtt                                                                                                                    <  2:992901/149‑1 (MQ=255)
                             cAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGtt                                                                                                                    <  3:36303/149‑1 (MQ=255)
                              aaaTCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTa                                                                                                                   <  1:283788/149‑1 (MQ=255)
                                aTCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAga                                                                                                                 <  2:938169/149‑1 (MQ=255)
                                     tGCCGAGATGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGAAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACAGTTAGatgatg                                                                                                            >  4:737397/1‑149 (MQ=255)
                                        cGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCtg                                                                                                         >  3:767592/1‑149 (MQ=255)
                                        cGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCtg                                                                                                         <  1:475803/149‑1 (MQ=255)
                                          aGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCtgtg                                                                                                       <  2:348227/149‑1 (MQ=255)
                                               gTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAgcg                                                                                                  >  2:127835/1‑149 (MQ=255)
                                                 gCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCt                                                                                                >  4:109193/1‑149 (MQ=255)
                                                 gCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCt                                                                                                >  1:132375/1‑149 (MQ=255)
                                                  cAGATGCGCTTGCTTGCCCTAGAACAATGGGGCATGCCTCGGACTTAAACGCAATGTGCTAAACCGGCCATCAATTCCTTGCACGATGGACGCAACTGGCAACGTATACCAGAACCCATGCGACTGTTAGATAATGCTGTGGAGCGCtc                                                                                               >  4:312898/1‑149 (MQ=255)
                                                  cAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCtc                                                                                               <  3:1080235/149‑1 (MQ=255)
                                                   aGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCtca                                                                                              >  4:1070763/1‑149 (MQ=255)
                                                    gATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCa                                                                                                                                     >  2:726833/1‑109 (MQ=255)
                                                    gATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCa                                                                                                                                     <  1:726833/109‑1 (MQ=255)
                                                     aTGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCtgtg                                                                                                       <  4:973137/138‑1 (MQ=255)
                                                     aTGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCtgtg                                                                                                       >  3:973137/1‑138 (MQ=255)
                                                       gcgcTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTcatcat                                                                                          >  3:675772/1‑149 (MQ=255)
                                                           ttgcttgcCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCatgatg                                                                                      >  4:590558/1‑149 (MQ=255)
                                                             gcttgcCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGAc                                                                                    >  2:777704/1‑149 (MQ=255)
                                                                   cccAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCAt                                                                                                                             <  3:47201/102‑1 (MQ=255)
                                                                   cccAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCAt                                                                                                                             >  4:47201/1‑102 (MQ=255)
                                                                   cccAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCtcat                                                                                             >  2:505575/1‑134 (MQ=255)
                                                                   cccAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCtcat                                                                                             <  1:505575/134‑1 (MQ=255)
                                                                    ccAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGc                                                                             >  4:606920/1‑149 (MQ=255)
                                                                     cAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCg                                                                            <  3:32597/149‑1 (MQ=255)
                                                                     cAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCg                                                                            >  1:791593/1‑149 (MQ=255)
                                                                     cAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCg                                                                            >  1:822726/1‑149 (MQ=255)
                                                                        aaCAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGata                                                                         <  3:566813/149‑1 (MQ=255)
                                                                           aaTGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCa                                                                      >  2:998392/1‑149 (MQ=255)
                                                                            aTGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCAt                                                                     >  1:622734/1‑149 (MQ=255)
                                                                             tGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCAtt                                                                    <  2:618299/149‑1 (MQ=255)
                                                                             tGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCAtt                                                                    <  1:685001/149‑1 (MQ=255)
                                                                             tGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCAtt                                                                    <  4:351630/149‑1 (MQ=255)
                                                                                         cTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTc                                                        <  3:495010/149‑1 (MQ=255)
                                                                                           gCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGt                                                      <  3:606920/149‑1 (MQ=255)
                                                                                            ccTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGtt                                                     <  2:247206/149‑1 (MQ=255)
                                                                                            ccTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGtt                                                     >  4:697750/1‑149 (MQ=255)
                                                                                             cTTTAACGCAAAGTTCTAAGCCGGTCATCAATGCCGAGCACGATGGTCGCCCCTGGCTACGAATACCGGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGttt                                                    <  4:130608/149‑1 (MQ=255)
                                                                                                tAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTgcg                                                 <  1:20373/149‑1 (MQ=255)
                                                                                                 aaCGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTgcgc                                                <  3:635072/149‑1 (MQ=255)
                                                                                                  aCGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCa                                               >  4:811511/1‑149 (MQ=255)
                                                                                                   cGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCAc                                              <  3:897737/149‑1 (MQ=255)
                                                                                                   cGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCAc                                              <  1:923663/149‑1 (MQ=255)
                                                                                                    gCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACt                                             >  2:246545/1‑149 (MQ=255)
                                                                                                         gTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGAcc                                                                                   <  2:466396/106‑1 (MQ=255)
                                                                                                         gTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGAcc                                                                                   >  1:466396/1‑106 (MQ=255)
                                                                                                          ttCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATAtt                                       >  4:640726/1‑149 (MQ=255)
                                                                                                          ttCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATAtt                                       >  4:887536/1‑149 (MQ=255)
                                                                                                          ttCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATAtt                                       <  1:1060620/149‑1 (MQ=255)
                                                                                                          ttCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATAtt                                       <  4:1041309/149‑1 (MQ=255)
                                                                                                          ttCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATAtt                                       <  1:998392/149‑1 (MQ=255)
                                                                                                            cTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATAtttt                                     <  4:121051/149‑1 (MQ=255)
                                                                                                             tAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATAttttt                                    <  3:833580/149‑1 (MQ=255)
                                                                                                               aaCCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTcc                                  >  4:873860/1‑149 (MQ=255)
                                                                                                                    gTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTg                             >  1:497941/1‑149 (MQ=255)
                                                                                                                     tcatcaATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTgg                            <  2:631008/149‑1 (MQ=255)
                                                                                                                     tcatcaATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTgg                            <  3:121380/149‑1 (MQ=255)
                                                                                                                      catcaATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTggg                           <  3:109193/149‑1 (MQ=255)
                                                                                                                       atcaATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGc                          <  3:409832/149‑1 (MQ=255)
                                                                                                                         caATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCAc                                              <  1:118068/127‑1 (MQ=255)
                                                                                                                         caATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCAc                                              >  2:118068/1‑127 (MQ=255)
                                                                                                                          aaTGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTAt                       <  1:617964/149‑1 (MQ=255)
                                                                                                                          aaTGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATc                      <  2:292675/149‑1 (MQ=255)
                                                                                                                           aTGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATc                      <  3:389655/149‑1 (MQ=255)
                                                                                                                           aTGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATc                      <  4:695546/149‑1 (MQ=255)
                                                                                                                           aTGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATc                      <  4:849531/149‑1 (MQ=255)
                                                                                                                               cGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCa                                                                               <  4:440122/88‑1 (MQ=255)
                                                                                                                               cGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCa                                                                               >  3:440122/1‑88 (MQ=255)
                                                                                                                                gAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGgcg                 >  4:27055/1‑149 (MQ=255)
                                                                                                                                gAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGgcg                 <  3:442036/149‑1 (MQ=255)
                                                                                                                                 aGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGgcgc                >  2:81758/1‑149 (MQ=255)
                                                                                                                                   cACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCAtt                                                                    >  3:15606/1‑95 (MQ=255)
                                                                                                                                   cACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCAtt                                                                    <  4:15606/95‑1 (MQ=255)
                                                                                                                                       ttcgTCGCCAGTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCACTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGGCGCGGCAct          >  1:936127/4‑149 (MQ=255)
                                                                                                                                        tGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGGCGCGGCActc         <  1:127835/149‑1 (MQ=255)
                                                                                                                                           tCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGGCGCGGCACTCttt      >  4:1041296/1‑149 (MQ=255)
                                                                                                                                            cGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGGCGCGGCACTCTTTg     >  4:233197/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATGATGCTGTGGAGCGCTCATCATGATGACCATCAGCGATATCATTGAAATTATTGTCGTTTGCGCACTGATATTTTTCCCGCTGGGCTATCTGGCGCGGCACTCTTTGCGA  >  NC_000913/3697824‑3698115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: