Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,918,916 T→A 5.5% K314N (AAA→AAT atpA ← F1 sector of membrane‑bound ATP synthase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,918,9160TA 5.5% 477.6 / 8.3 139K314N (AAA→AATatpAF1 sector of membrane‑bound ATP synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (57/73);  new base A (4/4);  total (62/77)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

CCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGTT  >  NC_000913/3918770‑3919064
                                                                                                                                                  |                                                                                                                                                    
ccGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCttt                                                                                                                                                    >  2:521299/1‑149 (MQ=255)
ccGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCttt                                                                                                                                                    <  2:572985/149‑1 (MQ=255)
ccGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCttt                                                                                                                                                    <  3:1999/149‑1 (MQ=255)
   gCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGt                                                                                                                                                 >  4:582963/1‑149 (MQ=255)
      ttGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGaa                                                                                                                                              <  3:234544/149‑1 (MQ=255)
       tGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAg                                                                                                                                             <  3:459891/149‑1 (MQ=255)
       tGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCAAAGGTGAAg                                                                                                                                             <  2:852407/149‑1 (MQ=255)
       tGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCAAAGGTGAAg                                                                                                                                             <  4:1005793/149‑1 (MQ=255)
            aGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTc                                                                                                                                        <  1:1039022/149‑1 (MQ=255)
            aGGTTGGTTTCCAGGAAGAGCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCAAAGGTGAAgtgaaa                                                                                                                                        <  4:283726/149‑6 (MQ=255)
              gttggttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCaa                                                                                                                                      <  4:693658/149‑1 (MQ=255)
              gttggttTCCAGGAAGATCTGACCATCGGGAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCaa                                                                                                                                      >  3:877894/1‑149 (MQ=255)
              gttagtcTCGAGGCAGATCTGACCCTCGGTATTGGAGACTACGTTGGCCGGAACGAACGCAGAAACATCACCAGCCTGAGTTTTGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTGCACTTCACCGTTGGTGAGGGCTTCaa                                                                                                                                      >  2:376997/1‑149 (MQ=255)
               ttggttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTg                                                                                                                                                   >  2:612260/1‑135 (MQ=255)
                tggttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACg                                                                                                                                    <  3:749141/149‑1 (MQ=255)
                   tttCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTAt                                                                                                                                 >  3:33813/1‑149 (MQ=255)
                   tttCCAGGAAGATCTGACCATCGGTAATGGACATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCAGCAGTGCGGTCAGAGAACCGTTTTTCCCTTTCGCTTCACCTTTGGTGAAGGCTTCAACGTAt                                                                                                                                 >  3:666899/1‑149 (MQ=255)
                     tCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTc                                                                                                                               >  1:154708/1‑149 (MQ=255)
                        aGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGc                                                                                                                            >  3:809512/1‑149 (MQ=255)
                        aGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGc                                                                                                                            >  2:926485/1‑149 (MQ=255)
                          gAAGTTCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAGGGCTTCAACGTATTCGGCGt                                                                                                                          >  4:1073339/1‑149 (MQ=255)
                          gAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGt                                                                                                                          >  1:193965/1‑149 (MQ=255)
                           aaGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGtt                                                                                                                         <  1:293756/149‑1 (MQ=255)
                             gATCTGACCATCGGTAATGGAGATTACGTTGGTCAGAACGAACGCAGAAACGTAACCCGCCTGAGCTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTaa                                                                                                                       <  2:777278/149‑1 (MQ=255)
                               tCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAaca                                                                                                                     <  1:859586/149‑1 (MQ=255)
                                   aCCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTg                                                                                                                 <  2:127598/149‑1 (MQ=255)
                                     cATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgca                                                                                                               <  3:582963/149‑1 (MQ=255)
                                     cATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgca                                                                                                               <  4:441098/149‑1 (MQ=255)
                                     cATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgca                                                                                                               >  3:359892/1‑149 (MQ=255)
                                             aTGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTc                                                                                                       >  4:219746/1‑149 (MQ=255)
                                              tGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTcc                                                                                                      <  3:889849/149‑1 (MQ=255)
                                               ggAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCca                                                                                                     <  1:729289/149‑1 (MQ=255)
                                                   aTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCcagcag                                                                                                 <  1:885277/149‑1 (MQ=255)
                                                    ttACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGa                                                                                                <  2:418404/149‑1 (MQ=255)
                                                    ttACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGa                                                                                                >  3:570301/1‑149 (MQ=255)
                                                     tACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGAc                                                                                               <  2:187497/149‑1 (MQ=255)
                                                       cGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTCAACACGTGCAGCACGCTCCAGCAGACga                                                                                             <  3:927612/149‑1 (MQ=255)
                                                         ttGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCAAAGGTGAAgt                                                                                                                                            >  2:324849/1‑99 (MQ=255)
                                                          tGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGagag                                                                                          <  4:223905/149‑1 (MQ=255)
                                                          tGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGagag                                                                                          <  3:50721/149‑1 (MQ=255)
                                                             tCGGAACGAACGCAGAAACGTCACCCGCGTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAAACGGTTTTCCCTTTTACTTCACCTCTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGAAGAGAGTgg                                                                                       >  4:409642/1‑149 (MQ=255)
                                                             tCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGGGAGCgg                                                                                       >  4:441630/1‑149 (MQ=255)
                                                               ggAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAg                                                                                     >  3:994755/1‑149 (MQ=255)
                                                               ggAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAg                                                                                     <  3:1005865/149‑1 (MQ=255)
                                                                gaacgaacGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAgg                                                                                    >  4:1057448/1‑149 (MQ=255)
                                                                gaacgaacGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAgg                                                                                    >  2:203070/1‑149 (MQ=255)
                                                                        gCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCttt                                                                                                                                                    <  4:893040/77‑1 (MQ=255)
                                                                          aGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGt                                                                          >  1:989020/1‑149 (MQ=255)
                                                                          aGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGt                                                                          >  1:464634/1‑149 (MQ=255)
                                                                          aGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGt                                                                          <  1:521299/149‑1 (MQ=255)
                                                                           gAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTc                                                                         <  3:937999/149‑1 (MQ=255)
                                                                           gAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTc                                                                         <  3:785768/149‑1 (MQ=255)
                                                                                gTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCg                                                                    >  4:756550/1‑149 (MQ=255)
                                                                                   aCCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGa                                                                 >  1:154997/1‑149 (MQ=255)
                                                                                    cccGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGaa                                                                <  1:434791/149‑1 (MQ=255)
                                                                                     ccGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTc                                                                                                                                        >  3:490062/1‑76 (MQ=255)
                                                                                     ccGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTCTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAAt                                                               >  2:169400/1‑149 (MQ=255)
                                                                                         cTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCnt                                                           <  3:219746/149‑1 (MQ=255)
                                                                                           gAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCa                                                         <  2:193965/149‑1 (MQ=255)
                                                                                            aGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCAc                                                        <  4:539630/149‑1 (MQ=255)
                                                                                              tttCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCg                                                                    >  2:180035/1‑135 (MQ=255)
                                                                                              tttCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGt                                                      >  1:73503/1‑149 (MQ=255)
                                                                                                tCGATAATCGGCGGTGGGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGGGAAGGTTTCAACGTATTCGGCGTTAACACGTGCGGCACGCCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCt                                                   <  4:1035744/149‑1 (MQ=255)
                                                                                                  gATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTg                                                  >  4:672192/1‑149 (MQ=255)
                                                                                                    tAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGc                                                <  4:773553/149‑1 (MQ=255)
                                                                                                    tAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGc                                                <  4:570301/149‑1 (MQ=255)
                                                                                                       tCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCTCCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGga                                             <  3:1073339/149‑1 (MQ=255)
                                                                                                       tCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTa                                                                                  >  1:430965/1‑112 (MQ=255)
                                                                                                       tCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGga                                             >  4:966536/1‑149 (MQ=255)
                                                                                                          gCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGgacga                                          >  1:1050172/1‑149 (MQ=255)
                                                                                                          gCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGgacga                                          <  3:127887/149‑1 (MQ=255)
                                                                                                             gTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACgg                                       <  2:715007/149‑1 (MQ=255)
                                                                                                                 ggTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGagca                                   <  2:1081951/149‑1 (MQ=255)
                                                                                                                 ggTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGagca                                   <  2:187367/149‑1 (MQ=255)
                                                                                                                     agagAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGCAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGcagcag                               <  2:321978/149‑1 (MQ=255)
                                                                                                                      gagaACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAgg                              <  3:756550/149‑1 (MQ=255)
                                                                                                                         aaCCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGgag                           >  4:138687/1‑149 (MQ=255)
                                                                                                                             ggTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCt                       <  4:994755/149‑1 (MQ=255)
                                                                                                                             ggTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCt                       <  4:481366/149‑1 (MQ=255)
                                                                                                                               ttttCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGa                     <  2:73503/149‑1 (MQ=255)
                                                                                                                                 ttCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACg                   <  3:571726/149‑1 (MQ=255)
                                                                                                                                  tCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACgg                  <  2:464634/149‑1 (MQ=255)
                                                                                                                                  tCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACgg                  >  1:986434/1‑149 (MQ=255)
                                                                                                                                   cccTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGt                 <  1:203070/149‑1 (MQ=255)
                                                                                                                                   cccTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGt                 <  3:438944/149‑1 (MQ=255)
                                                                                                                                    ccTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCAAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGgacga                                          <  3:649978/123‑1 (MQ=255)
                                                                                                                                     cTTTGACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTaa               >  1:1034507/1‑149 (MQ=255)
                                                                                                                                     cTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCTGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTaa               >  2:713660/1‑149 (MQ=255)
                                                                                                                                      tttCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAg              >  1:267582/1‑149 (MQ=255)
                                                                                                                                       ttcacttcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACTTGCAGCACACTCCAGCATACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGc             <  4:877894/149‑1 (MQ=255)
                                                                                                                                         gccttcacCAAAGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCaa           >  4:289581/3‑149 (MQ=255)
                                                                                                                                         gccttcacCAAAGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCaa           >  3:874673/3‑149 (MQ=255)
                                                                                                                                            ttcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGcagcag                               >  4:639536/1‑126 (MQ=255)
                                                                                                                                            ttcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGcagcag                               <  3:639536/126‑1 (MQ=255)
                                                                                                                                             tcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACAGGTGCAGCACGCTCCAGCAGACGGGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGc       >  4:646797/1‑149 (MQ=255)
                                                                                                                                             tcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGc       <  2:435581/149‑1 (MQ=255)
                                                                                                                                              cacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGcc      <  1:7725/149‑1 (MQ=255)
                                                                                                                                               acCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCt     >  4:578332/1‑149 (MQ=255)
                                                                                                                                               acCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCt     >  4:418000/1‑149 (MQ=255)
                                                                                                                                                  tttGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGtt  >  3:43460/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGTT  >  NC_000913/3918770‑3919064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: