Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,223,855 G→A 6.0% A10T (GCG→ACG)  metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,223,8550GA 6.0% 374.4 / 8.4 116A10T (GCG→ACG) metHhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand):  ref base G (46/63);  new base A (2/5);  total (48/68)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

Only 100 of 121 total aligned reads displayed.

ATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCA  >  NC_000913/4223708‑4224002
                                                                                                                                                   |                                                                                                                                                   
aTGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcgtgc                                                                                                                                                    >  2:425704/1‑149 (MQ=255)
  gAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTgcgc                                                                                                                                                  <  4:848670/149‑1 (MQ=255)
     ttAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGt                                                                                                                                               <  3:275691/149‑1 (MQ=255)
     ttAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGt                                                                                                                                               >  1:448267/1‑149 (MQ=255)
      tAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGtt                                                                                                                                              <  3:1022870/149‑1 (MQ=255)
      tAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGtt                                                                                                                                              >  2:252926/1‑149 (MQ=255)
        aTGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGCACGCAGttgt                                                                                                                                            <  3:891769/149‑3 (MQ=255)
        aTGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGCACGCAGttgt                                                                                                                                            <  1:280227/149‑3 (MQ=255)
         tGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaaa                                                                                                                                           <  3:631117/149‑1 (MQ=255)
         tGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGCACGCAGttgtt                                                                                                                                           <  2:599116/149‑4 (MQ=255)
          gTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAAt                                                                                                                                          <  1:154485/149‑1 (MQ=255)
           ttGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATg                                                                                                                                         >  4:948626/1‑149 (MQ=255)
           ttGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATg                                                                                                                                         >  3:54211/1‑149 (MQ=255)
             gAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGaa                                                                                                                                       <  3:1004399/149‑1 (MQ=255)
              aaCAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAATGTTGAACAACTGCGTGCGCAGTTAAATGAAc                                                                                                                                      <  4:662487/149‑1 (MQ=255)
                    tctcATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTc                                                                                                                                >  3:543531/1‑149 (MQ=255)
                    tctcATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCATGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTc                                                                                                                                >  1:477584/1‑149 (MQ=255)
                      tcATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAAt                                                                                                                                          >  2:866967/1‑137 (MQ=255)
                      tcATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAAt                                                                                                                                          <  1:866967/137‑1 (MQ=255)
                       cATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTgg                                                                                                                             >  4:24015/1‑149 (MQ=255)
                        aTGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGt                                                                                                                            >  1:50040/1‑149 (MQ=255)
                           ttGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCt                                                                                                                         <  2:539612/149‑1 (MQ=255)
                            tGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTg                                                                                                                        <  1:425704/149‑1 (MQ=255)
                             gCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTgg                                                                                                                       >  1:157048/1‑149 (MQ=255)
                              cGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGa                                                                                                                      <  3:945907/149‑1 (MQ=255)
                               gtggtgGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGAc                                                                                                                     >  3:233964/1‑149 (MQ=255)
                                  gtggtCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGAAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGAcggc                                                                                                                  <  3:437032/149‑1 (MQ=255)
                                      tCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTa                                                                                                                                   >  2:543277/1‑128 (MQ=255)
                                      tCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTa                                                                                                                                   <  1:543277/128‑1 (MQ=255)
                                           tttACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGc                                                                                                         <  3:1067316/149‑1 (MQ=255)
                                                cacaGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCAt                                                                                                    >  3:229682/1‑149 (MQ=255)
                                                cacaGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCAt                                                                                                    <  3:212974/149‑1 (MQ=255)
                                                 acaGATGCGTTTATGCCAGTATGGTTTGCTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATg                                                                                                   >  4:113887/1‑149 (MQ=255)
                                                  caGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGa                                                                                                  <  4:658830/149‑1 (MQ=255)
                                                        cGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCAGCATGATCCaga                                                                                            <  3:848619/149‑1 (MQ=255)
                                                        cGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCaga                                                                                            <  2:157048/149‑1 (MQ=255)
                                                        cGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCATGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCaga                                                                                            <  2:477584/149‑1 (MQ=255)
                                                         gTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCagag                                                                                           <  1:692014/149‑1 (MQ=255)
                                                            tATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGTGCACCATGATCCAGAGTTa                                                                                        <  2:66479/149‑1 (MQ=255)
                                                             aTGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTCTTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTAt                                                                                       <  2:978031/149‑1 (MQ=255)
                                                                ccAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGa                                                                                    >  3:153679/1‑149 (MQ=255)
                                                                ccAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGa                                                                                    >  3:1072117/1‑149 (MQ=255)
                                                                ccAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGa                                                                                    >  2:190048/1‑149 (MQ=255)
                                                                   gaaTGGTTCGTTGAATTCTTATTAAACAAAGGTTGACCGTAACGGGAGCAAGTGTGAGCACCAAAGCGGAAGAACTGCGAGCGCAGCTACCTGAACGTATTCTGGTGCTGGACGGCGGTACGGGCACCATGATCCCGAGTTATCTACTg                                                                                 >  2:599373/3‑149 (MQ=255)
                                                                   gTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTg                                                                                 >  2:770894/1‑149 (MQ=255)
                                                                         tttGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGaa                                                                           <  2:864202/149‑1 (MQ=255)
                                                                           tgttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGc                                                                         <  4:852631/149‑1 (MQ=255)
                                                                           tgttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGc                                                                         <  2:912883/149‑1 (MQ=255)
                                                                             ttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCg                                                                       >  1:480223/1‑149 (MQ=255)
                                                                               gAATTTTTATTAAACCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGAt                                                                     >  3:190640/1‑149 (MQ=255)
                                                                                aaTTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGAtt                                                                    <  4:1037398/149‑1 (MQ=255)
                                                                                 aTTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGAttt                                                                   >  2:993638/1‑149 (MQ=255)
                                                                                  tttttATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGAtttt                                                                  >  4:252798/1‑149 (MQ=255)
                                                                                     ttattaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgt                                                               >  2:425274/1‑149 (MQ=255)
                                                                                     ttattaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgt                                                               <  2:50040/149‑1 (MQ=255)
                                                                                     ttattaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgt                                                               >  1:382759/1‑149 (MQ=255)
                                                                                      tattaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtg                                                              <  3:554887/149‑1 (MQ=255)
                                                                                       attaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtgg                                                             >  3:591995/1‑149 (MQ=255)
                                                                                       attaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtgg                                                             <  2:272200/149‑1 (MQ=255)
                                                                                             tCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACg                                                       >  1:1024084/1‑149 (MQ=255)
                                                                                                gggTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCtt                                                    <  1:637690/149‑1 (MQ=255)
                                                                                                 ggTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCttt                                                   >  2:713249/1‑149 (MQ=255)
                                                                                                  gTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTg                                                  <  2:190928/149‑1 (MQ=255)
                                                                                                  gTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTg                                                  <  4:54211/149‑1 (MQ=255)
                                                                                                        cGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACt                                            <  2:342688/149‑1 (MQ=255)
                                                                                                              gggAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCAt                                      >  3:991417/1‑149 (MQ=255)
                                                                                                                gAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGc                                    <  4:591995/149‑1 (MQ=255)
                                                                                                                  gCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGa                                  >  2:460396/1‑149 (MQ=255)
                                                                                                                     aGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCt                               >  3:1015412/1‑149 (MQ=255)
                                                                                                                       tgtgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCa                             >  1:395512/1‑149 (MQ=255)
                                                                                                                         tgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCaaa                           <  3:24015/149‑1 (MQ=255)
                                                                                                                         tgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCaaa                           <  1:535059/149‑1 (MQ=255)
                                                                                                                         tgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCaaa                           <  2:442868/149‑1 (MQ=255)
                                                                                                                          gAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAg                          <  1:890517/149‑1 (MQ=255)
                                                                                                                          gAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAg                          <  4:64725/149‑1 (MQ=255)
                                                                                                                          gAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAg                          <  1:605531/149‑1 (MQ=255)
                                                                                                                          gAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAg                          <  3:252798/149‑1 (MQ=255)
                                                                                                                          gAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAg                          <  2:794092/149‑1 (MQ=255)
                                                                                                                            gcagcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGc                        >  1:286473/1‑149 (MQ=255)
                                                                                                                            gcagcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGc                        <  3:508072/149‑1 (MQ=255)
                                                                                                                              agcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGcaa                      <  1:34155/149‑1 (MQ=255)
                                                                                                                               gcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGcaac                     >  4:628348/1‑149 (MQ=255)
                                                                                                                               gcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGcaac                     <  2:867841/149‑1 (MQ=255)
                                                                                                                                caAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCg                                                                <  1:883206/105‑1 (MQ=255)
                                                                                                                                caAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCg                                                                >  2:883206/1‑105 (MQ=255)
                                                                                                                                caAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGcaaca                    <  3:923412/149‑1 (MQ=255)
                                                                                                                                    gTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGa                <  4:1072117/149‑1 (MQ=255)
                                                                                                                                     tGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGAc                                 >  1:1039526/1‑131 (MQ=255)
                                                                                                                                     tGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGAc                                 <  2:1039526/131‑1 (MQ=255)
                                                                                                                                     tGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGAc               <  2:395512/149‑1 (MQ=255)
                                                                                                                                       gAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACct             <  4:630729/149‑1 (MQ=255)
                                                                                                                                       gAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACct             >  3:213852/1‑149 (MQ=255)
                                                                                                                                       atttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACct             >  2:759692/5‑149 (MQ=255)
                                                                                                                                       atttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACct             >  2:511580/5‑149 (MQ=255)
                                                                                                                                        aacaacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGTCTTAACGAAGACGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctg            >  3:256629/1‑149 (MQ=255)
                                                                                                                                         acaacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctgc           <  4:1064227/149‑1 (MQ=255)
                                                                                                                                           aacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctgctg         >  4:854914/1‑149 (MQ=255)
                                                                                                                                           aacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctgctg         <  1:511580/149‑1 (MQ=255)
                                                                                                                                            acTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTgg        <  4:297666/149‑1 (MQ=255)
                                                                                                                                                  tgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCa  <  2:480223/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCA  >  NC_000913/4223708‑4224002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: