Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,592,201 T→A 5.5% V182D (GTC→GAC)  tsr → methyl‑accepting chemotaxis protein I, serine sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,592,2010TA 5.5% 497.9 / 12.3 144V182D (GTC→GAC) tsrmethyl‑accepting chemotaxis protein I, serine sensor receptor
Reads supporting (aligned to +/- strand):  ref base T (65/70);  new base A (4/4);  total (69/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 145 total aligned reads displayed.

AATGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCAT  >  NC_000913/4592056‑4592349
                                                                                                                                                 |                                                                                                                                                    
aaTGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACTAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTGCGAGAAGAAGTATGTGGCTTACATAGAGCAAAACGATCGGCTCCATGATATCGCCGGCAg                                                                                                                                                   <  4:300443/149‑1 (MQ=255)
 aTGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGc                                                                                                                                                  <  4:525066/149‑1 (MQ=255)
      cTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATaa                                                                                                                                             <  4:307732/149‑1 (MQ=255)
       tGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGTCTCCATGATATCGCCGTCAGCGATAAc                                                                                                                                            >  4:933734/1‑149 (MQ=255)
        ggCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCTGACGGCGATATCa                                                                                                                                           <  3:620642/149‑1 (MQ=255)
         gCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCTGACGGCGATATCat                                                                                                                                          <  3:509532/149‑2 (MQ=255)
         gCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGGATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACaa                                                                                                                                          <  2:981689/149‑1 (MQ=255)
          cGGAGGTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGTCGCTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAAt                                                                                                                                         <  4:701250/149‑1 (MQ=255)
           ggAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCTGACGGCGATatcatgg                                                                                                                                        <  2:301795/149‑7 (MQ=255)
            gAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGc                                                                                                                                       <  3:1001123/149‑1 (MQ=255)
             aGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGcc                                                                                                                                      <  4:445808/149‑1 (MQ=255)
              gCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGcct                                                                                                                                     >  2:197589/1‑149 (MQ=255)
              gCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGcct                                                                                                                                     <  3:229830/149‑1 (MQ=255)
              gCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGcct                                                                                                                                     >  1:1000363/1‑149 (MQ=255)
                 gATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGcctcct                                                                                                                                  >  2:350251/1‑149 (MQ=255)
                    ccAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACa                                                                                                                               >  4:728089/1‑149 (MQ=255)
                    ccAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACa                                                                                                                               <  1:565488/149‑1 (MQ=255)
                      aaCTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGc                                                                                                                             >  3:524340/1‑149 (MQ=255)
                      aaCTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGc                                                                                                                             >  1:154390/1‑149 (MQ=255)
                       aCTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGcc                                                                                                                            >  1:382359/1‑149 (MQ=255)
                                tGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGAtgt                                                                                                                   <  4:233409/149‑1 (MQ=255)
                                   aGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGa                                                                                                                <  3:889126/149‑1 (MQ=255)
                                     gCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGAtt                                                                                                              <  1:796228/149‑1 (MQ=255)
                                       aaaaTCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCt                                                                                                            >  1:1041012/1‑149 (MQ=255)
                                       aaaaTCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGCGGATTCt                                                                                                            >  1:1026359/1‑149 (MQ=255)
                                        aaaTCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCtg                                                                                                           <  3:272801/149‑1 (MQ=255)
                                         aaTCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCtgg                                                                                                          <  4:320002/149‑1 (MQ=255)
                                          aTCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCtggt                                                                                                         <  2:893722/149‑1 (MQ=255)
                                          aTCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCtggt                                                                                                         <  1:166599/149‑1 (MQ=255)
                                             aaCGAGTGCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCGGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTggg                                                                                                      >  2:666011/1‑149 (MQ=255)
                                               cGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCg                                                                                                    >  2:197631/1‑149 (MQ=255)
                                                 tgTTCTTTGATCATCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATGGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGtg                                                                                                  >  4:935876/2‑149 (MQ=255)
                                                  gTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGtga                                                                                                 <  3:35909/149‑1 (MQ=255)
                                                  gTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGtga                                                                                                 <  4:826640/149‑1 (MQ=255)
                                                  gTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGtga                                                                                                 <  3:423340/149‑1 (MQ=255)
                                                  gTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGtga                                                                                                 <  1:661451/149‑1 (MQ=255)
                                                     cTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGtgatga                                                                                              >  3:618283/1‑149 (MQ=255)
                                                      tttGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATaa                                                                                                                                             >  2:112313/1‑101 (MQ=255)
                                                      tttGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGAtgt                                                                                                                   >  3:651865/1‑127 (MQ=255)
                                                      tttGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGAtgt                                                                                                                   <  4:651865/127‑1 (MQ=255)
                                                      tttGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTgatgat                                                                                             >  2:598172/1‑149 (MQ=255)
                                                      tttGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTgatgat                                                                                             >  4:475528/1‑149 (MQ=255)
                                                      tttGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTgatgat                                                                                             >  4:629964/1‑149 (MQ=255)
                                                      tttGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTgatgat                                                                                             >  1:382372/1‑149 (MQ=255)
                                                      tttGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTgatgat                                                                                             >  1:287684/1‑149 (MQ=255)
                                                       ttGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGAtc                                                                                            <  4:1028106/149‑1 (MQ=255)
                                                         gATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGAtcgt                                                                                          <  1:706469/149‑1 (MQ=255)
                                                         gATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGAtcgt                                                                                          <  1:176467/149‑1 (MQ=255)
                                                          aTCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGAtcttc                                                                                         <  4:724897/149‑1 (MQ=255)
                                                          aTCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGAtcgtc                                                                                         <  2:1026359/149‑1 (MQ=255)
                                                             aGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCtggt                                                                                                         <  4:471876/130‑1 (MQ=255)
                                                             aGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTa                                                                                      <  3:377247/149‑1 (MQ=255)
                                                              gcggACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTAc                                                                                     >  2:81817/4‑149 (MQ=255)
                                                                    ccAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCgg                                                                               >  1:1015395/1‑149 (MQ=255)
                                                                    ccAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCgg                                                                               <  2:382359/149‑1 (MQ=255)
                                                                      aGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTc                                                                             >  4:838093/1‑149 (MQ=255)
                                                                      aGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTc                                                                             >  2:747415/1‑149 (MQ=255)
                                                                      aGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTc                                                                             >  4:337071/1‑149 (MQ=255)
                                                                      aGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTc                                                                             >  2:117430/1‑149 (MQ=255)
                                                                        ggATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGtg                                                                                                  <  1:750436/126‑1 (MQ=255)
                                                                        ggATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGtg                                                                                                  >  2:750436/1‑126 (MQ=255)
                                                                             tCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCtg                                                                                                           >  3:628894/1‑112 (MQ=255)
                                                                               aGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGtgatg                                                                                               <  3:240601/122‑1 (MQ=255)
                                                                               aGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGtgatg                                                                                               >  4:240601/1‑122 (MQ=255)
                                                                                 gACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGt                                                                  >  1:222503/1‑149 (MQ=255)
                                                                                      tttCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGt                                                             >  4:194616/1‑149 (MQ=255)
                                                                                          gagaAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCgg                                                         >  3:722643/1‑149 (MQ=255)
                                                                                          gagaAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCgg                                                         >  2:1018702/1‑149 (MQ=255)
                                                                                          gagaAGCAGTATGTGGCTTAAAAGGAGCAAAGCGATCGGCTACATGATATCGCCGTCAGCGATAACAATGCCTCATACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCAGTCATCTTCGCCGTCTGGTTCgg                                                         <  4:1026964/149‑1 (MQ=255)
                                                                                           agaAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGt                                                        <  3:728089/149‑1 (MQ=255)
                                                                                           agaAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGt                                                        <  1:583027/149‑1 (MQ=255)
                                                                                            gaAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCAATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTa                                                       >  1:945287/1‑149 (MQ=255)
                                                                                             aaGCTGTAAGTGGCTTACATGGTGCAAAACGCTCGGCTCCATGATATCGCCGTCAGCCATAACAATGCCTCCTACAGCCCGGCGATGGTGATTCTGGTGGGCGCTGTGATCGTCGTACTGGCGGTCAGCTTCGCCGTCTGGTTCGGTAt                                                      >  4:201585/1‑149 (MQ=255)
                                                                                                  gTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGTGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAg                                                 <  3:998720/149‑1 (MQ=255)
                                                                                                     tgtgGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCt                                              >  3:1065940/1‑149 (MQ=255)
                                                                                                      gtgGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGAGAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTc                                             <  1:1015652/149‑1 (MQ=255)
                                                                                                       tgGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTaaa                                                  <  2:879951/143‑1 (MQ=255)
                                                                                                         gCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCt                                          >  1:133653/1‑149 (MQ=255)
                                                                                                          cTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATTGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTg                                         <  4:713614/149‑1 (MQ=255)
                                                                                                           ttACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTgg                                        >  1:227885/1‑149 (MQ=255)
                                                                                                                tGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGAtcgtc                                                                                         <  3:212825/95‑1 (MQ=255)
                                                                                                                  gAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGcc                                 >  2:103928/1‑149 (MQ=255)
                                                                                                                  gAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGcc                                 >  1:540362/1‑149 (MQ=255)
                                                                                                                    gCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCaa                               <  3:481233/149‑1 (MQ=255)
                                                                                                                       aaaCGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAgcgc                                  >  3:69685/1‑143 (MQ=255)
                                                                                                                       aaaCGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAgcgc                                  <  4:69685/143‑1 (MQ=255)
                                                                                                                        aaCGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGaa                           <  1:81817/149‑1 (MQ=255)
                                                                                                                         aCGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAAt                          <  3:475528/149‑1 (MQ=255)
                                                                                                                                    cATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGa               >  3:1012317/1‑149 (MQ=255)
                                                                                                                                    attgttatCGCTGACGGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGa               >  4:1011008/6‑149 (MQ=255)
                                                                                                                                    attgttatCGCTGACGGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGa               >  2:389533/6‑149 (MQ=255)
                                                                                                                                    attgttatCGCGGACGGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGa               >  1:576246/6‑149 (MQ=255)
                                                                                                                                     aTGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGAc              <  2:256926/149‑1 (MQ=255)
                                                                                                                                      tGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACa             <  3:933133/149‑1 (MQ=255)
                                                                                                                                        ttatCGCTGACGGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTa                                      >  3:85358/2‑122 (MQ=255)
                                                                                                                                        ttatCGCTGACGGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTa                                      <  4:85358/121‑1 (MQ=255)
                                                                                                                                            cGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTc       <  3:194616/149‑1 (MQ=255)
                                                                                                                                             gCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCg      >  4:891121/1‑149 (MQ=255)
                                                                                                                                               cGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTc    <  3:456207/149‑1 (MQ=255)
                                                                                                                                                gTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCa   <  4:524340/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AATGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCAT  >  NC_000913/4592056‑4592349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: