Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,2680TC23.1% 98.7 / 18.7 56intergenic (+474/+168)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (17/23);  new base C (7/5);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAA  >  NC_000913/4296010‑4296540
                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                     
aGCGTGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCTACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCa                                                                                                                                                                                                                                                                       <  2:110325/275‑1 (MQ=25)
      aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCACCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGTGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGCTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTTACGCGTCCTATTAGGCTTACGTCCGCCAGAGCAAGTGCCCGTTTTCGCGTGCTTTTGTAGGTCGGATAAGGCGGTCACGCTCCATCCGatcgcaatgtat                                                                                                                                                                                                                                                                 >  2:537123/1‑264 (MQ=11)
      aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGCTAAGGCGTTTATGCCGAATACGACATCAAGGCATGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCACGACAGCGAAATCACCTGAGTAAGGCTGCATTTGTCGGTGTGATCAGGCCTTTAACGCGCATCCGatactcaatctt                                                                                                                                                                                                                                                                 >  4:158252/1‑264 (MQ=11)
      aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCATACAGCGCAATAGCCTGATTTCGCGTGCTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATCAATGCCt                                                                                                                                                                                                                                                                 >  2:539994/1‑275 (MQ=255)
       ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt                                                                                                                                                                                                                                                                 >  3:52299/1‑274 (MQ=37)
                 cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACCTCAATGCCTGATGCGAcctt                                                                                                                                                                                                                                                      >  3:539779/1‑272 (MQ=255)
                    ctagggCGTTTACGCCGCATCGTGCATCAACGCATGATGCGAGACTGGCCGCGTCTTACTAGGCGTAAGCCAGAAAGCGAAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGAGAAGGCGTTTATGCCGAATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGGCTACGCCAGAAAGCGCAATAGCCTGATTTGGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCttgc                                                                                                                                                                                                                                                   <  2:343840/271‑3 (MQ=11)
                          cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                                                                                                                                              <  1:52299/274‑1 (MQ=21)
                              tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                                                                                                                                         >  3:237250/1‑275 (MQ=17)
                                      aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCCGACAGGGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGGTTACGCCGCCTCAGCCACCATTGCCTGATTCGACGCTTGCAGCTTCTTATCAGGCCTag                                                                                                                                                                                                                                >  4:348070/1‑275 (MQ=11)
                                                                                                 cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGTTTTTTTAGGTCGGATAAGGCGTTTa                                                                                                                                                                       >  3:625524/1‑274 (MQ=14)
                                                                                                                                               tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACCCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTAAGCGTGATTCTGTCGGTCGGATAGGGCCTTTACGC‑C‑GACCCCAACATCAACGCCTGTCACGACGCTTGCCCCCTCCTATc                                                                                                                     >  4:572828/1‑276 (MQ=11)
                                                                                                                                                                               ctcttgccgggTCTTATAAGGCCTACGCCAGACCTCGAAATAGGCTGATTTTGCGTGATTTTGTAGGTCGGATAAGGCGTTTCTGCCGCATCCGACATCAACGCCTGATGCGACTCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCa                                                                                     <  3:158158/265‑1 (MQ=11)
                                                                                                                                                                               cGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCa                                                                                     <  1:625524/275‑1 (MQ=255)
                                                                                                                                                                                gctttctgcgTCTTATCAGTCATACGACAGACAGCGCATTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCaaa                                                                                   <  2:348070/269‑1 (MQ=11)
                                                                                                                                                                                 cttgccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCaaa                                                                                   <  1:411994/270‑1 (MQ=255)
                                                                                                                                                                                    gccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGAGGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAACCCTT‑GCTCAATTTTTTAAActc                                                                                >  4:546935/3‑275 (MQ=11)
                                                                                                                                                                                          tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTcac                                                                           >  1:554051/1‑274 (MQ=255)
                                                                                                                                                                                            ttATCAGGACTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCTTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACAccc                                                                       <  2:364014/276‑1 (MQ=255)
                                                                                                                                                                                            ttACCAGGCCTCCGCCACACAGCGAAATAGCCTGATTGAGCTGTATTTTTTAGGTCGAATAAGGCGGTTAGGCCGCATCCTACATCAACGCATGATGCGACGCATAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACAcc                                                                        <  1:456833/275‑1 (MQ=14)
                                                                                                                                                                                             tATCGGGCCTACGCAGGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACAccc                                                                       <  1:476902/275‑1 (MQ=255)
                                                                                                                                                                                             tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACAcc                                                                        <  1:314578/274‑1 (MQ=255)
                                                                                                                                                                                             tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGACGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACAccc                                                                       <  1:449369/275‑1 (MQ=255)
                                                                                                                                                                                             tATCAGGCCTACGCCAGAAAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCg                                                                      <  2:534021/276‑1 (MQ=255)
                                                                                                                                                                                             tATCAGGCCTACGCAGGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACAcc                                                                        <  1:256431/274‑1 (MQ=255)
                                                                                                                                                                                             tATCAGGACTACTCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACAcc                                                                        <  1:545846/274‑1 (MQ=255)
                                                                                                                                                                                                  ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAACCGTT‑GTTTAATTTTTCAAACTCTTCACACCCGGTa                                                                   >  1:154735/1‑274 (MQ=255)
                                                                                                                                                                                                     cTACGCCATAGCGCGCAATAGCCTTATTTAGCGGGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTATCGCGTCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                              <  4:125748/276‑1 (MQ=14)
                                                                                                                                                                                                      taaccccgACACCGCAATGGCCTGATTTAGCTGGATTTTGTAGGGCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAGGCTACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                              <  2:274875/268‑1 (MQ=14)
                                                                                                                                                                                                      tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                              <  2:74827/275‑1 (MQ=255)
                                                                                                                                                                                                       aCGCCAGACAGCGAAGTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                              <  1:623800/274‑1 (MQ=255)
                                                                                                                                                                                                        cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATAAAAcc                                                            >  2:521187/1‑275 (MQ=255)
                                                                                                                                                                                                                 gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                                                    <  2:704438/274‑1 (MQ=255)
                                                                                                                                                                                                                  cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATCAGGCGTTTA‑‑CGCTGCATCCGACCTCAATGACTGATGCGCCGCTTGCCGCGTCTTTTCACGCCTATCTTAACCGTTGGGTCATTTTTTCTACCTC‑TCACACCCGGTATCACATCCTTcccttcc                                                  >  4:179077/1‑270 (MQ=11)
                                                                                                                                                                                                                  cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa                                                  >  1:497951/1‑275 (MQ=255)
                                                                                                                                                                                                                  cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGCTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                   >  1:192471/1‑274 (MQ=255)
                                                                                                                                                                                                                  cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CGCAGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATATTAACCGTA‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCATACATCCAg                                                 >  2:332259/1‑276 (MQ=255)
                                                                                                                                                                                                                    cAATAGGCTGATTTAGCGTGATTTTGTAGCTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                               <  2:471191/276‑1 (MQ=255)
                                                                                                                                                                                                                    cAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGAGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                               <  2:572828/276‑1 (MQ=255)
                                                                                                                                                                                                                     caTAGCCTGATTTAGCGGGAGTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGAGATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCCGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                               <  2:179077/274‑1 (MQ=255)
                                                                                                                                                                                                                     aagcgCCTGATTTAGCGTGATTTTGTCGGTCGTATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCGTACCGCGTCTGATCAGGCCTACGCCAGACCGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                               <  1:182044/271‑1 (MQ=17)
                                                                                                                                                                                                                     aaTATCCTGATTTAGCGTGATTTTGTAGGTCGGTTAAGGCCTTTAGAGCGCAGCCGACATCAACACCAGATGCGACTCTAAACGCGTCTTATCAGGACTACGCCAGACAGCGCAATAGCCTGATTTAGCATGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCAACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                               <  1:320285/275‑1 (MQ=17)
                                                                                                                                                                                                                            tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTTTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTc                                        >  2:95901/1‑275 (MQ=255)
                                                                                                                                                                                                                            tGATGTAGCGTGATTTTGTAGGACGGAGAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTGAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAAGGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                          <  2:1686/273‑1 (MQ=255)
                                                                                                                                                                                                                               tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAAtttt                                                                                        >  4:229539/1‑224 (MQ=255)
                                                                                                                                                                                                                                  aGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCa                                                                                     <  2:229539/224‑1 (MQ=255)
                                                                                                                                                                                                                                     gTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGtcc                               >  3:356108/1‑273 (MQ=255)
                                                                                                                                                                                                                                        aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTTACACCCGGTATCAAACCCTTCCATACATCTCAGGTTCAACCatgtcagc                            >  3:542023/1‑268 (MQ=255)
                                                                                                                                                                                                                                        aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCGTATCAGGCCTCACTTAACCGGT‑GGTTACTTTTTCAAACTCTTCACCACCGGTATCCACACCTTACATACCGCTCAGGTTACACCAGTACAgc                            >  4:171548/1‑275 (MQ=14)
                                                                                                                                                                                                                                           ttGTAGGTCGTATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                        <  2:179458/276‑1 (MQ=255)
                                                                                                                                                                                                                                           ttGTAGGGCGGATAGCGCGTGTAGTGCGCATCCGGCATCAACGCCTGATTCGTCGCTTAACGCGTGTTATTAGGCCTACGCCAGACAGCGCAAGAGCCTGATTTAGCGTGATGTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                        <  4:146433/276‑1 (MQ=18)
                                                                                                                                                                                                                                                  tCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTcac                                                                           >  2:576811/1‑218 (MQ=255)
                                                                                                                                                                                                                                                   cGGATAATGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTTATCTAGCGTGATTTTGTAGGTCGGATACGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACTCTTGCCTCGTCTTCTCATGCATATCTTACCCGTT‑GTTTAATTTTTCAAACCCTTCAACCCCGGTATCATACCCTTACATACCCCTCAGGCGCAACCTTTTCACCCCCTTTTCCTTg                >  4:577198/1‑276 (MQ=14)
                                                                                                                                                                                                                                                   cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTg                >  2:110107/1‑276 (MQ=255)
                                                                                                                                                                                                                                                     gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACAcc                                                                        <  4:576811/218‑1 (MQ=255)
                                                                                                                                                                                                                                                                 aTGCCTCATCCGACATCAACGCCTGATGCGCCGCTTACCGCGTCTTATCAGGCCTACGCCAGACAACGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaa  <  2:257754/276‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaa  <  1:356108/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                     
AGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT‑GGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAA  >  NC_000913/4296010‑4296540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑