BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,145,313	C→A	S683Y (TCC→TAC)	yegE →	putative diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,145,313	0	C	A	100.0%	451.2 / NA	131	S683Y (TCC→TAC)	yegE	putative diguanylate cyclase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (69/62); total (69/62)

Only 100 of 131 total aligned reads displayed.

TTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATG  >  NC_000913/2145051‑2145585

      ggtCCGCTCCGGACGGTGTTGCATATTACTTTTGGCGAAAACGGACCATTAATGGAGAACATTTAAAGTGCCGACACCTCACGTTCCACGATCGAGCAAGATGTGGGGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGc                                                                                                                                                                                                                                                                  <  3:246348/272‑1 (MQ=255)

        tCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGACTCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTCACGCAtct                                                                                                                                                                                                                                                              >  2:69645/1‑276 (MQ=255)

              ccggACGGTGTTGCATATTACTTTTGTCGACAACGGACCATTAATGGATACCATTTACAGTTCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCaa                                                                                                                                                                                                                                                        <  2:573567/273‑1 (MQ=255)

               cTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGGAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCaa                                                                                                                                                                                                                                                        <  3:174928/275‑1 (MQ=255)

                                tACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCCTCTCGCCAACCGCGCCAGTTTTtaga                                                                                                                                                                                                                                       >  2:188261/1‑275 (MQ=255)

                                caCTTTTGGCGACAACGGACGATTAATGGAGAACATTGCCAGGTGCGAAACCTCAAGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCAGGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTgaga                                                                                                                                                                                                                                       <  4:521677/274‑1 (MQ=255)

                                 aCTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGAGCCTACCATGATGCACTGACGCATCTCGCCAACCGGGCCAGTTTTgcg                                                                                                                                                                                                                                        >  1:246963/1‑271 (MQ=255)

                                  cTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGaaa                                                                                                                                                                                                                                     <  1:638413/275‑1 (MQ=255)

                                    tttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCGCAGGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAaaca                                                                                                                                                                                                                                   <  4:152966/275‑1 (MQ=255)

                                    tgttgCGAAAACGGAACATTGATGTAGAACATTTACAGTGCCGACACCTCACGTTCCGCGTGCGAGCAAGATGTGGTGTGGCACTGTCGGGGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCGGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAaaca                                                                                                                                                                                                                                   <  4:482320/271‑1 (MQ=255)

                                     ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCCGTTTTGTGacac                                                                                                                                                                                                                                    >  2:674147/1‑273 (MQ=255)

                                       ggCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCCCGCAAAATGCTGCGCCAGCTGATCTACAGCGCCTACCGTGATGCACTGACGCCTCTCGCAAACCGCGCCAGATTTGAGATACAACt                                                                                                                                                                                                                                >  2:67502/1‑275 (MQ=255)

                                       ggCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACACCtc                                                                                                                                                                                                                               >  2:416701/1‑275 (MQ=255)

                                                     agtAATGGAGAAGATTTACCGTGCCGACCCCGCACGTTCCGGGATCGAGCAAGTTGTGGTGTTGCACTGTCCGAGCGGCGGCAGTTACGACGTGCATTACAGGATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCtgctgc                                                                                                                                                                                                                  <  4:457953/273‑1 (MQ=255)

                                                      ttAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCtgctgc                                                                                                                                                                                                                  >  1:265670/1‑274 (MQ=255)

                                                       tatgggataccATTTACAGTGCCGCAACCTCACGTTAGGCGATAGAGCAAGATTGGGTGTTGCACTGTCGGAGAGGCGGCAGTTACGACGTGGATTACAGTATTACGCCGTTAAGTACTCTGAACGGCAGAAATATTAGTTCGGTTCTGTTGATTCAGGAAGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCaa                                                                                                                                                                                                                <  3:472455/265‑1 (MQ=255)

                                                       tAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCaaa                                                                                                                                                                                                               >  2:672141/1‑276 (MQ=255)

                                                            gcgaACATTTACACGGCCGAGACCTCGCGTTCCGCGATCGAGCAAGATGTGGTGTTGCAATGTCGGAGCCGCGGCAGTTACGTCGTGCATTACAGTATTACGCCGTTAAGTACGCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTa                                                                                                                                                                                                          <  4:188261/274‑1 (MQ=255)

                                                            gagaACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGCTGCACTGACGCATCTCGCCACCCGCGCCAGTTTTGAGCAACAACTGCGTATCCTTCTGCCACCgg                                                                                                                                                                                                            >  1:598858/1‑274 (MQ=255)

                                                              gaACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAACCAACTGCGTTTCCTGCTGCAAACGGtca                                                                                                                                                                                                         >  2:611208/1‑273 (MQ=255)

                                                              gaACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTa                                                                                                                                                                                                          >  1:570116/1‑274 (MQ=255)

                                                              gaACATTTACAGTACCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATCTTGGTTCGGTTCTGGTGATTCAGGCCGTTACCGAATCACGCAAAACGCTGCCCCAGCTGACCTACAGCGCCTACAATGTTGCACTGACGCATCCCCCCAACCGCGCCAGGTTTGAGAAACAACTGCGTATCCTGCTCCAAACGGTa                                                                                                                                                                                                          >  3:22923/1‑274 (MQ=255)

                                                               aaCATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                        <  2:495133/275‑1 (MQ=255)

                                                               aaCATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAAGATTGGTTCGGTTCTGGTGATTCAGTACGTTACCGAATCACGCAAAAGGCTGTGCCAGCTCAGCTACAGCGCCTACGATGATGCACTAACGCATCGCGCAAAACGCGCCAGTTTTGTGTACCCACTGCGTATCCTGCTGCAAACGCTaa                                                                                                                                                                                                         >  3:654449/1‑274 (MQ=255)

                                                                aCATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGATGAGCTACAGCGCCTACCATGATGCACTGCCGAATCTCGCCAACCCCGACAGTTTTGCGAAACAACTGCGTATCCTGCTGCAACCGGTAAACa                                                                                                                                                                                                      >  2:236647/1‑276 (MQ=255)

                                                                   tgtcgcgTGCCGACACCACGCTTTCCTGTGGCGAGCAGGATGTGGTGGTTCTCTGGACGAGCGCTGGCATGTCCCAAGAGCATGACAGTTTTACGGCTTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTGTTGGCGCTTCATGACGTTCCCGAATCACGCAAAATGCTGCGCCAGCAGCGCTACCGCGCCTAACATGTTGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGATACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                   <  4:535028/270‑1 (MQ=255)

                                                                    tttaggTGCCGAAACCTGCCGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTTTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                   <  3:128885/270‑1 (MQ=255)

                                                                    ttACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACCGCGCCTACCATGATGCACTGACGCATCCCGCCAACCGCGCCAGTTTTGAGACACAACTGCGTATACTGCTGCAAACGGTAAACAGTac                                                                                                                                                                                                  >  2:107829/1‑276 (MQ=255)

                                                                         gTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCCATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCTCCTACCATGATGCACTGACGCATCACGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTACCCTGCTGCCACCGGTAAACAGTAcccaa                                                                                                                                                                                              >  3:280788/1‑274 (MQ=255)

                                                                         gTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCAtc                                                                                                                                                                                                                                                               >  2:339608/1‑210 (MQ=255)

                                                                         ctGCCGACACCTCACGTTCCGCGATCTAGGAAGATGTGGTGTTGCACTGACGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCGGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAGACAACTGCGTATCCTGCTGCAAACGGTAAACAGTAcaca                                                                                                                                                                                               <  2:1220/273‑1 (MQ=255)

                                                                           gCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCa                                                                                                                                                                                            <  2:289697/275‑1 (MQ=255)

                                                                            ccGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCg                                                                                                                                                                                                                                                            <  4:339608/210‑1 (MQ=255)

                                                                               acacCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTTCGCCAGCTGATCTACAGCGCCTACCATGATGCACTGACGCATCTCGCAAACCGCCCCATGTTTGAGAACCAACTGCGTTTCCTGCTGCAAAGGGTAAACAGTAAACCTCcgcgc                                                                                                                                                                                        >  3:314204/1‑274 (MQ=255)

                                                                                cacCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                                                                                                                                                       <  1:141777/275‑1 (MQ=255)

                                                                                 acCTCACGGTCCGCGAGCGAGCAAGATGGGGTGTTGCACTGTCGGAGCGGCGGCAGTTAAGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACa                                                                                                                                                                                      <  1:534279/275‑1 (MQ=255)

                                                                                    tCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATgac                                                                                                                                                                                  >  2:148592/1‑274 (MQ=255)

                                                                                        gTTCCGCGATCGAGCAAGATTTGTTGGTGCACTGTCGCAGCGGCGACAGTTACGACGTGAATTACAGTTTTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCt                                                                                                                                                                               <  3:280455/274‑1 (MQ=255)

                                                                                        gTTCCGCGATCGAGCAAGAGGTGGGGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑cc                                                                                                                                                                                 <  2:308176/272‑1 (MQ=255)

                                                                                                                   tGCCCTGTCGGAGCGGCGGCCGTTACGACGTTCATTCCAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTTGTTCGGTTCTTGTGATTCAGGCCGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCTCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                  <  2:27491/276‑1 (MQ=255)

                                                                                                                     cACTGTCGAGGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAAGCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                  <  1:150871/274‑1 (MQ=255)

                                                                                                                          tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTTAGACACAACAGCGTATCCTGACGCCAACGGTTAACAGTAAAACAGAACGACATGTCCAGGGTTTTTCACGA‑CCTGCTCGCTTTAAAGGGGTGaa                                                                                                                                           >  4:333591/1‑276 (MQ=255)

                                                                                                                          tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCACACGGTAAACAGTACACATCAGCGCCATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGa                                                                                                                                            >  2:49205/1‑275 (MQ=255)

                                                                                                                          tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACgtt                                                                                                                                                                                                           >  2:369359/1‑211 (MQ=255)

                                                                                                                          tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGa                                                                                                                                            >  2:489600/1‑275 (MQ=255)

                                                                                                                          tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGGTTTTATCGATCTTGATCCCTTTAAAGAGGtaa                                                                                                                                            >  3:29489/1‑273 (MQ=255)

                                                                                                                                           acgacgTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCAt                                                                                                                           <  2:429541/275‑1 (MQ=255)

                                                                                                                                                 tGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGACCTTGATCGCTTTAAAGCGGAGAATGACAGCGCTTGGCATgcg                                                                                                                        >  1:381754/1‑272 (MQ=255)

                                                                                                                                                           tATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACACG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGCAAGCTGCGGGCATgcggccgttgcctctt                                                                                                           >  3:110704/1‑264 (MQ=255)

                                                                                                                                                                cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTtgctgc                                                                                                     >  2:193512/1‑276 (MQ=255)

                                                                                                                                                                 ggcGTGAAGGACTTTGGACGGCAGCAATTTTGGTTCGGTTCTGGTGATTAAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACNTCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTgcg                                                                                                    <  4:374711/274‑1 (MQ=255)

                                                                                                                                                                  ccGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTtgctgc                                                                                                     >  1:184391/1‑274 (MQ=255)

                                                                                                                                                                   cGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGTTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACCTT‑CCCTGGTGTTTATCGATCt                                                                                                                                                                >  1:456181/1‑214 (MQ=255)

                                                                                                                                                                     ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGaa                                                                                                >  4:634679/1‑276 (MQ=255)

                                                                                                                                                                     ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGaa                                                                                                >  2:582443/1‑276 (MQ=255)

                                                                                                                                                                     ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTAAACATCAGCGACATC‑CCCTGGTGTTTAACGAACTTGATCGCTTTAAAGCGGTGAATGAAAGAGCCGGGCATGCGGCGGGTGAGGCTTTGCTCGGCGa                                                                                                 >  4:522970/1‑275 (MQ=255)

                                                                                                                                                                     ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCCGACCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAATAGTACACATCAGCGACATG‑CCCTGGTGGTTCTCGATCTTGATCGCTTTCACGCGGTGAATGACAGCGCCGGGCTTGCGTCGGGTGACGCTTTGCTGCGCGa                                                                                                 >  1:104942/1‑275 (MQ=255)

                                                                                                                                                                      tAAGTACTCGTGACGGCAGCAATTTTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGcgcg                                                                                                  <  3:184391/273‑1 (MQ=255)

                                                                                                                                                                       agggaCTCTGGACGGCAGCAATATTGATTCGGTTCTGGTGATTCAGGACGTTACCGAATCACAAAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCGTGATGCACTGACGCATCTCGCCAAGCGGGCCAGTTTTGGGGAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAAATG‑CCCTGGTGTTTATCGATCTTGAt                                                                                                                                                            <  3:456181/210‑1 (MQ=255)

                                                                                                                                                                          tacccgggACGGCAGCATTATTGGTTCGGTTCTGGGGAGTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                           <  4:148592/270‑1 (MQ=255)

                                                                                                                                                                          tACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                           <  2:400623/276‑1 (MQ=255)

                                                                                                                                                                           aCTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                           <  3:570116/275‑1 (MQ=255)

                                                                                                                                                                               tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGTACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGGTTAGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CGCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGCAGCTTTGCTGCGCGAACTGTcttcgt                                                                                      >  2:602628/1‑276 (MQ=255)

                                                                                                                                                                                  aCGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGcgtcgt                                                                                      >  2:448365/1‑273 (MQ=255)

                                                                                                                                                                                    ggCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAAt                                                                                  >  1:608309/1‑275 (MQ=255)

                                                                                                                                                                                    ggCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACACTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTTATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTCCTTCCCGAACGGGCGTCGTTacg                                                                                  >  3:308227/1‑273 (MQ=255)

                                                                                                                                                                                     gcagcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATg                                                                                 <  1:29489/275‑1 (MQ=255)

                                                                                                                                                                                      cagcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGATTTGCTGCGCGAACTGGCTTCGTTAATGcc                                                                               >  2:317042/1‑275 (MQ=255)

                                                                                                                                                                                       agcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGCCAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTg                                                                              >  2:238111/1‑276 (MQ=255)

                                                                                                                                                                                        gcaATTTTGGTTCGGTTCTGGTGATTCAGGACGTTACGGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                           <  1:308227/262‑1 (MQ=255)

                                                                                                                                                                                        gcaATATTGGTTCGGGTCTGGCGAGTCAGGACGTTACGCAATCACGCAAAATGCGCCGGCAGCTGAGCTACAGCGCGTACCATGATGCACTGACGCATCTCGCCACCCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCAAGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTg                                                                              <  4:236647/275‑1 (MQ=255)

                                                                                                                                                                                                ggttcggttcTGGTGATTCAGGACGTTACCGAATCCCGCAAACTGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTAtgctg                                                                     <  2:121248/276‑1 (MQ=255)

                                                                                                                                                                                                      gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                           >  1:472569/1‑248 (MQ=255)

                                                                                                                                                                                                        tcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGTTACAGCGCCTACCAGGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTGAGCAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGGTCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGtt                                                                 <  1:22923/272‑1 (MQ=255)

                                                                                                                                                                                                           ggTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGc                                                                                                                                                 >  1:534598/1‑189 (MQ=255)

                                                                                                                                                                                                            gTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCg                                                           >  1:327827/1‑274 (MQ=255)

                                                                                                                                                                                                               aTTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTg                                                                                                                                             <  3:534598/189‑1 (MQ=255)

                                                                                                                                                                                                                ttCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCAGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTg                                                      <  3:265670/275‑1 (MQ=255)

                                                                                                                                                                                                                 tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCTTTCCAGCGACGTGc                                                     >  1:95722/1‑275 (MQ=255)

                                                                                                                                                                                                                 tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGc                                                     >  3:496447/1‑275 (MQ=255)

                                                                                                                                                                                                                 tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGAAAGCGCCGGGCATCCGGCGGGTGACGCTTTGCTGCGCGAACTTGCGTCGTTAATGCTGAGTATGCTGAGTTCCAGCgcagcgct                                                    >  4:681814/1‑269 (MQ=255)

                                                                                                                                                                                                                      aCGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGgcg                                                <  3:327827/275‑1 (MQ=255)

                                                                                                                                                                                                                        gctACCGCAGCACGCAAATTCCTGCGCCAGCTGAGATACAGCGCCTACCATGATGCACGGACGAATCTCGCGAACCGCGCCAGTGTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAGCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACGGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGa                                             <  2:333591/274‑1 (MQ=255)

                                                                                                                                                                                                                         ttACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGCCGTGCTGGCGCCAc                                            >  2:634787/1‑276 (MQ=255)

                                                                                                                                                                                                                         gtcacGAGTCACGCAAAATGCTTCCCCAGCTGGGCTACAGCGCCTACCATGATGCACCGACGCATCCCGCCAACCGCGCCCGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGTTCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGa                                             <  1:654449/271‑1 (MQ=255)

                                                                                                                                                                                                                          tACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAACCAACTGCGTATCCTGCTGCAAACGGTAAACAGTATACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCCGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTTTGCCGCGTTCCAGTGACctgctgcgcggcc                                            >  3:475416/1‑268 (MQ=255)

                                                                                                                                                                                                                            ccGAATAACGCAAAATGCTGCGCCAGCTGAGTTACAGCGCATACCATGATGCACTGACGCATCTCGCCAACCGGGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACt                                           <  1:5461/274‑1 (MQ=255)

                                                                                                                                                                                                                               cataccccAAACTGCTGAGCCACCTGAGCTACAGCGACTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTg                                      <  4:582443/269‑1 (MQ=255)

                                                                                                                                                                                                                                    cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTggg                                    >  1:334001/1‑273 (MQ=255)

                                                                                                                                                                                                                                    cGCAAAATGCTGCACGAGCTGAGCTACAGCGCCTACCAGTATGCACTGATGCATCTCGCCAACCGCGCCAGTTTTGAGGAACAACAGCTTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGa                                  <  2:661890/275‑1 (MQ=255)

                                                                                                                                                                                                                                    acaaaaaTGCTGGACCGGCTGAGCAACCGCGCCTAGCATGAAGCGATTAAGCGTCGGGCCAAGCGCGCCAGTTTAGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTTATCGCTTTAAAGCGGTGAATGACGGGGCCGGGCGTGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGa                                  <  3:381754/272‑1 (MQ=255)

                                                                                                                                                                                                                                               ggtccAGCTGCGCTACTGAGCCTACCATGATGAACTGAGGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCGGGTGTTTATCGATCTTGGTCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGAAGCTTTGCTGGGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGt                        <  4:44426/271‑1 (MQ=255)

                                                                                                                                                                                                                                               gcgcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTg                                      >  1:46784/1‑260 (MQ=255)

                                                                                                                                                                                                                                                 gcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                    <  2:191070/276‑1 (MQ=255)

                                                                                                                                                                                                                                                  ccAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                    <  1:33500/275‑1 (MQ=255)

                                                                                                                                                                                                                                                  ccAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                    <  1:496447/275‑1 (MQ=255)

                                                                                                                                                                                                                                                   cAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGa                                  <  3:46784/260‑1 (MQ=255)

                                                                                                                                                                                                                                                          gCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCa            <  2:589968/275‑1 (MQ=255)

                                                                                                                                                                                                                                                                gagcCTACCAGGGTGCACTTCGGCAACTCGCCAACCGCGCCAGTTTTGAGAACAAACGGCGTATCCTGCTGAAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTCTCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGt      <  3:181997/273‑1 (MQ=255)

                                                                                                                                                                                                                                                                   ccTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATgcgg                                                                                                                       >  3:641137/1‑159 (MQ=255)

TTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATG‑CCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATG  >  NC_000913/2145051‑2145585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`