Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,970,817:1 +G 8.0% coding (685/1131 nt) wecB → UDP‑N‑acetyl glucosamine‑2‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,970,8171.G 8.0% 416.2 / 33.5 100coding (685/1131 nt)wecBUDP‑N‑acetyl glucosamine‑2‑epimerase
Reads supporting (aligned to +/- strand):  ref base . (46/46);  new base G (4/4);  total (50/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

Only 100 of 104 total aligned reads displayed.

GGTGCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGT  >  NC_000913/3970669‑3970962
                                                                                                                                                     |                                                                                                                                                 
ggTGCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGACACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACgcgc                                                                                                                                                    >  1:284456/1‑149 (MQ=255)
 gTGCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCt                                                                                                                                                  >  2:90011/1‑149 (MQ=255)
 gTGCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCt                                                                                                                                                  <  1:215476/149‑1 (MQ=255)
   gCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTgg                                                                                                                                                <  2:135058/149‑1 (MQ=255)
    cGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGc                                                                                                                                               <  4:414445/149‑1 (MQ=255)
     gTGACCAGGTGATGGGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCa                                                                                                                                              >  3:45884/1‑149 (MQ=255)
     gTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGATCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCa                                                                                                                                              >  4:468995/1‑149 (MQ=255)
        aCCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGAc                                                                                                                                           <  2:421718/149‑1 (MQ=255)
        aCCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCAGCGCGTGGCAGat                                                                                                                                           <  4:249418/149‑2 (MQ=255)
        aCCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCAGCGCGTGGCAGat                                                                                                                                           <  3:438728/149‑2 (MQ=255)
        aCCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCAGCGCGTGGCAGat                                                                                                                                           <  2:4171/149‑2 (MQ=255)
           aGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATc                                                                                                                                        <  1:147591/149‑1 (MQ=255)
              tgatgaGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGcc                                                                                                                                     <  1:501967/149‑1 (MQ=255)
                  gaGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGccacca                                                                                                                                 >  3:354311/1‑149 (MQ=255)
                     cagcGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGc                                                                                                                              >  1:287853/1‑149 (MQ=255)
                         gACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCa                                                                                                                          >  2:342667/1‑149 (MQ=255)
                          aCAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAg                                                                                                                         >  4:329153/1‑149 (MQ=255)
                          aCAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAg                                                                                                                         <  3:450388/149‑1 (MQ=255)
                            aaGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGa                                                                                                                       <  4:485597/149‑1 (MQ=255)
                               cTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTTTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACAt                                                                                                                    <  4:545239/149‑1 (MQ=255)
                                tGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATc                                                                                                                   <  3:70144/149‑1 (MQ=255)
                                 gCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGTTGGCAGACATCGCCACCACGCACCAGGACATcc                                                                                                                  <  3:391673/149‑1 (MQ=255)
                                   gTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAg                                                                                                                <  4:229750/149‑1 (MQ=255)
                                      cAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGAtt                                                                                                             <  1:363367/149‑1 (MQ=255)
                                       aGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGGCCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTg                                                                                                            >  1:408222/1‑149 (MQ=255)
                                        gAACTGGCGGCAAATTACCCGTTTATCGATCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGt                                                                                                           <  3:468995/149‑1 (MQ=255)
                                        gAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGt                                                                                                           <  3:374473/149‑1 (MQ=255)
                                         aaCTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTc                                                                                                          <  3:444565/149‑1 (MQ=255)
                                         aaCTGGCGGCAAATTACCCGTTTATCGAACCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCGTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTc                                                                                                          >  4:380682/1‑149 (MQ=255)
                                          aCTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCt                                                                                                         <  1:68093/149‑1 (MQ=255)
                                          aCTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCt                                                                                                         <  1:432553/149‑1 (MQ=255)
                                            tGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTAt                                                                                                       <  2:319592/149‑1 (MQ=255)
                                                ggcAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCgg                                                                                                   <  2:287853/149‑1 (MQ=255)
                                                      ttACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCAtc                                                                                             >  3:224339/1‑149 (MQ=255)
                                                      ttACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCAtc                                                                                             >  3:54963/1‑149 (MQ=255)
                                                         cccGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCa                                                                                          >  4:487568/1‑149 (MQ=255)
                                                           cGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAAc                                                                                        <  3:44244/149‑1 (MQ=255)
                                                            gTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAAcc                                                                                       <  2:146875/149‑1 (MQ=255)
                                                             tttATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAAccc                                                                                      >  3:144904/1‑149 (MQ=255)
                                                                    aCCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTc                                                                               >  1:329292/1‑149 (MQ=255)
                                                                       ccGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCaga                                                                            <  1:90011/149‑1 (MQ=255)
                                                                       ccGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTGTCGGTCGTGGCGTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGCTTGTCTATCCGGTGCATCTCAACCCGAACGTCaga                                                                            >  2:390310/1‑149 (MQ=255)
                                                                        cGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCagag                                                                           >  3:82695/1‑149 (MQ=255)
                                                                          aTAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGaa                                                                         <  1:571782/149‑1 (MQ=255)
                                                                            aaaaaGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAAcc                                                                       >  2:361862/1‑149 (MQ=255)
                                                                             aaaaGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCg                                                                      <  3:600566/149‑1 (MQ=255)
                                                                             aaaaGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCg                                                                      <  1:15285/149‑1 (MQ=255)
                                                                             aaaaGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATACGGTGCATCTCAACCCGAACGTCAGAGAACCg                                                                      <  2:488268/149‑1 (MQ=255)
                                                                               aaGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGTCTTTGAAGAAATCTGCCACGCGCTGACAGACATCGCCACCACTCACCAGGACATTCAGATTTTCTATCCGGTGCATCTCAACCTGAACGTCAGAGAACCGGt                                                                    <  2:284456/149‑1 (MQ=255)
                                                                               aaGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGt                                                                    <  1:342667/149‑1 (MQ=255)
                                                                                      ttCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGc                                                             >  1:195343/1‑149 (MQ=255)
                                                                                      ttCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGc                                                             >  4:427237/1‑149 (MQ=255)
                                                                                      ttCTGGTGACCGGTCACAGGCGTGAGAGGTTCCGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCAAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGc                                                             >  4:380562/1‑149 (MQ=255)
                                                                                       tCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCa                                                            <  3:216743/149‑1 (MQ=255)
                                                                                             gACCGGTCACAGGCGTGACAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGACTATCCGGAGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCCCATTCTga                                                      >  2:299437/1‑148 (MQ=255)
                                                                                               ccGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCACATTCTgggg                                                    >  2:488525/1‑149 (MQ=255)
                                                                                                    cacaGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCAtgt                                               >  2:98566/1‑149 (MQ=255)
                                                                                                    cacaGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCAtgt                                               <  2:420216/149‑1 (MQ=255)
                                                                                                     acaGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTgg                                                      <  3:451367/141‑1 (MQ=255)
                                                                                                     acaGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTgg                                                      >  4:451367/1‑141 (MQ=255)
                                                                                                       aGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGaa                                            >  3:459138/1‑149 (MQ=255)
                                                                                                       aGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGaa                                            >  4:559143/1‑149 (MQ=255)
                                                                                                        ggCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGaaa                                           >  2:112819/1‑149 (MQ=255)
                                                                                                        ggCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGaaa                                           >  2:308550/1‑149 (MQ=255)
                                                                                                         gCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGaaaa                                          >  2:483984/1‑149 (MQ=255)
                                                                                                           gTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAAt                                        >  1:123340/1‑149 (MQ=255)
                                                                                                            tGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATg                                       >  3:430814/1‑149 (MQ=255)
                                                                                                            tGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATg                                       >  1:68148/1‑149 (MQ=255)
                                                                                                              agggTTTCGCTCGTGGCTTAGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTc                                     >  2:473857/4‑149 (MQ=255)
                                                                                                                agTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCAt                                   <  4:224339/149‑1 (MQ=255)
                                                                                                                 gTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCAtt                                  >  2:472755/1‑149 (MQ=255)
                                                                                                                 gTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCAtt                                  <  1:81466/149‑1 (MQ=255)
                                                                                                                  tttCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCAtt                                  >  3:294335/1‑148 (MQ=255)
                                                                                                                  tttCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCAtt                                  <  4:294335/148‑1 (MQ=255)
                                                                                                                  tttCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTc                                 >  4:452078/1‑149 (MQ=255)
                                                                                                                       gTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgatc                            <  1:98566/149‑1 (MQ=255)
                                                                                                                        tCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCa                                    >  1:193258/1‑140 (MQ=255)
                                                                                                                        tCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCa                                    <  2:193258/140‑1 (MQ=255)
                                                                                                                           tGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgatcgatc                        >  1:209475/1‑149 (MQ=255)
                                                                                                                             gCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATc                                                               >  2:149503/1‑108 (MQ=255)
                                                                                                                             gCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATc                                                               <  1:149503/108‑1 (MQ=255)
                                                                                                                               tttGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACGTCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCa                    <  1:37488/149‑1 (MQ=255)
                                                                                                                                ttGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAg                   >  1:360892/1‑149 (MQ=255)
                                                                                                                                  gaagaaATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGa                 >  3:245293/1‑149 (MQ=255)
                                                                                                                                  gaagaaATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGa                 <  4:354311/149‑1 (MQ=255)
                                                                                                                                  gaagaaATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGa                 >  4:462688/1‑149 (MQ=255)
                                                                                                                                    agaaATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTATATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGt               >  1:283695/1‑149 (MQ=255)
                                                                                                                                      aaaTCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTAt             >  1:291497/1‑149 (MQ=255)
                                                                                                                                      aaaTCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTAt             <  1:390310/149‑1 (MQ=255)
                                                                                                                                       tgtCTGCCAGCGCGTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTAtt            >  4:264760/3‑149 (MQ=255)
                                                                                                                                       tgtCTGCCAGCGCGTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTAtt            >  3:429546/3‑149 (MQ=255)
                                                                                                                                        gtCTGCCAGCGCGTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTa              >  4:171986/2‑146 (MQ=255)
                                                                                                                                        gtCTGCCAGCGCGTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTa              <  3:171986/145‑1 (MQ=255)
                                                                                                                                         tCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTa          >  2:289407/1‑149 (MQ=255)
                                                                                                                                           tGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTAcc        <  3:487568/149‑1 (MQ=255)
                                                                                                                                             ccACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGt      <  1:158372/149‑1 (MQ=255)
                                                                                                                                              cACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCa                                    <  4:144238/118‑1 (MQ=255)
                                                                                                                                              cACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCa                                    >  3:144238/1‑118 (MQ=255)
                                                                                                                                               aCGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGttt    <  3:216980/149‑1 (MQ=255)
                                                                                                                                                 gcgcTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGt  <  3:462688/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GGTGCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGT  >  NC_000913/3970669‑3970962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: