New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3820576182 (2.730)3 (0.050) 3/248 NT 1.6% coding (595/1683 nt) ligB DNA ligase, NAD(+)‑dependent
?NC_000913 = 3820614 189 (2.950)coding (557/1683 nt) ligB DNA ligase, NAD(+)‑dependent
Rejected: Frequency below/above cutoff threshold.

GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCATCAAGCCAGCAACTTTTGCGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3820425‑3820576
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgcgAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAAGTTGCTGGCTTGATGATGCGCCAGGACGATAGCGACACGCTGAATTCTCTGGGCGTTTTTGTCTGGGCATGGCCGGATGGACCGCAGTTAATGTCTGATC  <  NC_000913/3820614‑3820468
                                                                                                                                                                                                                                                                                                           
GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCATCAAGCCAGCAACTTTTGC                                                                                                                                                        >  2:342518/1‑149
 TCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCATCAAGCCAGCAACTTTTGCG                                                                                                                                                       >  4:142857/1‑149
 TCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCATCAAGCCAGCAACTTTTGCG                                                                                                                                                       >  4:284649/1‑149
   TGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCATCAAGCCAGCAACTTTTGCGCG                                                                                                                                                     >  2:37412/1‑149
                                                   ATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCATCAAGCCAGCAACTTTGGCGCGAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAAG                                                                                                     <  1:564068/149‑1
                                                                CATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCATCAAGCCAGCAACTTTTGCGCGAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAA                                                                                                      <  3:571661/135‑1
                                                                CATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCATCAAGCCAGCAACTTTTGCGCGAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAA                                                                                                      >  4:571661/1‑135
                                                                                                                                                    CGCGAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAAGTTGCTGGCTTGATGATGCGCCAGGACGATAGCGACACGCTGAATTCTCTGGGCGTTTTTGTCTGGGCGTGGCCGGATGGACCGCAGTTAATGTCTGA    <  3:73267/149‑1
                                                                                                                                                     GCGAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAAGTTGCTGGCTTGATGATGCGCCAGGACGATAGCGACACGCTGAATTCTCTGGGCGTTTTTGTCTGGGCATGGCCGGATGGACCGCAGTTAATGTCTGAT   <  2:164081/149‑1
                                                                                                                                                     GCGAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAAGTTGCTGGCTTGATGATGCGCCAGGACGATAGCGACACGCTGAATTCTCTGGGCGTTTTTGTCTGGGCATGGCCGGATGGACCGCAGTTAATGTCTGAT   >  3:467459/1‑149
                                                                                                                                                     GCGAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAAGTTGCTGGCTTGATGATGCGCCAGGACGATAGCGACACGCTGAATTCTCTGGGCGTTTTTGTCTGGGCATGGCCGGATGGACCGCAGTTAATGTCTGAT   <  4:569696/149‑1
                                                                                                                                                      CGAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGTAAAAGTTGCTGGCTTGATGATGCGCCAGGACGATAGCGACACGCTGAATTCTCTGGGCGTTTTTGTCTGGGCATGGCCGGATGGACCGCAGTTAATGTCTGATC  <  3:394103/149‑1
                                                                                                                                                      CGAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAAGTTGCTGGCTTGATGATGCGCCAGGACGATAGCGACACGCTGAATTCTCTGGGCGTTTTTGTCTGGGCATGGCCGGATGGACCGCAGTTAATGTCTGATC  <  4:427748/149‑1
                                                                                                                                                                                                                                                                                                           
GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCATCAAGCCAGCAACTTTTGCGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3820425‑3820576
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgcgAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAAGTTGCTGGCTTGATGATGCGCCAGGACGATAGCGACACGCTGAATTCTCTGGGCGTTTTTGTCTGGGCATGGCCGGATGGACCGCAGTTAATGTCTGATC  <  NC_000913/3820614‑3820468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.