Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,201,015 A→T 5.4% T6T (ACT→ACA xisE ← e14 prophage; putative excisionase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,201,0150AT 5.4% 260.9 / 4.9 74T6T (ACT→ACAxisEe14 prophage; putative excisionase
Reads supporting (aligned to +/- strand):  ref base A (26/44);  new base T (2/2);  total (28/46)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

CCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGTTATTAACC  >  NC_000913/1200867‑1201163
                                                                                                                                                    |                                                                                                                                                    
ccccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAaga                                                                                                                                                      <  4:122556/149‑1 (MQ=255)
ccccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAaga                                                                                                                                                      <  1:302141/149‑1 (MQ=255)
ccccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAaga                                                                                                                                                      >  1:211201/1‑149 (MQ=255)
ccccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGCACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAaga                                                                                                                                                      <  1:445786/149‑1 (MQ=255)
 cccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAagag                                                                                                                                                     <  3:186414/149‑1 (MQ=255)
 cccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAagag                                                                                                                                                     >  1:220818/1‑149 (MQ=255)
 cccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAaga                                                                                                                                                      <  3:549462/148‑1 (MQ=255)
 cccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAaga                                                                                                                                                      >  4:549462/1‑148 (MQ=255)
  ccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGt                                                                                                                                                    >  2:232491/1‑149 (MQ=255)
  ccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGt                                                                                                                                                    <  1:611301/149‑1 (MQ=255)
    aactaactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTa                                                                                                                                                  >  1:396592/1‑149 (MQ=255)
             ggCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGaa                                                                                                                                         <  1:228487/149‑1 (MQ=255)
                   cTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCatat                                                                                                                                   >  2:515642/1‑149 (MQ=255)
                   cTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCatat                                                                                                                                   <  4:581236/149‑1 (MQ=255)
                    tCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATg                                                                                                                                  >  4:406663/1‑149 (MQ=255)
                    tCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATg                                                                                                                                  >  1:77131/1‑149 (MQ=255)
                        gCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGAt                                                                                                                              <  1:199531/149‑1 (MQ=255)
                        gCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGAt                                                                                                                              <  4:279460/149‑1 (MQ=255)
                                 aTAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatt                                                                                                                     <  1:561470/149‑1 (MQ=255)
                                  tAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatta                                                                                                                    >  3:615672/1‑149 (MQ=255)
                                  ccaCCGGCCATGAAACAAGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatta                                                                                                                    >  1:503986/3‑149 (MQ=255)
                                     ccGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtacta                                                                                                                 <  2:77131/149‑1 (MQ=255)
                                     ccGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtacta                                                                                                                 <  2:77043/149‑1 (MQ=255)
                                     ccGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtacta                                                                                                                 <  1:232491/149‑1 (MQ=255)
                                      cGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtactac                                                                                                                >  3:319632/1‑149 (MQ=255)
                                      cGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtactac                                                                                                                >  2:82264/1‑149 (MQ=255)
                                         ccTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACt                                                                                                             >  4:235067/1‑149 (MQ=255)
                                              tttCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTACCTg                                                                                                        <  1:507107/149‑1 (MQ=255)
                                               ttCATGGCCGGTGGACAAAGTAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGc                                                                                                       <  4:423021/149‑1 (MQ=255)
                                                tCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCt                                                                                                      <  1:176000/149‑1 (MQ=255)
                                                 cATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGAt                                                                                                                              >  4:217903/1‑124 (MQ=255)
                                                 cATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGAt                                                                                                                              <  3:217903/124‑1 (MQ=255)
                                                  aTGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTa                                                                                                    <  3:235067/149‑1 (MQ=255)
                                                    ggCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGt                                                                                                  <  3:221969/149‑1 (MQ=255)
                                                     gCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGtc                                                                                                 >  3:600983/1‑149 (MQ=255)
                                                       cGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGtctc                                                                                               <  1:82264/149‑1 (MQ=255)
                                                        ggTGGATAAAATAAATTCTGCTTAGCATAACGCCGCAATGTGGACACACTTGTAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCGCCTTATTACTACACTAACTGCTTAGTCTCa                                                                                              <  2:298410/149‑1 (MQ=255)
                                                        ggTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCa                                                                                              <  1:344218/149‑1 (MQ=255)
                                                               aaaaTAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATc                                                                                       <  2:507987/149‑1 (MQ=255)
                                                                  ataaatTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatta                                                                                                                    >  3:433594/1‑117 (MQ=255)
                                                                  ataaatTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatta                                                                                                                    <  4:433594/117‑1 (MQ=255)
                                                                  ataaatTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGAc                                                                                    >  2:19050/1‑149 (MQ=255)
                                                                  ataaatTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGAc                                                                                    >  4:583304/1‑149 (MQ=255)
                                                                            cTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCAcggt                                                                          >  1:504316/1‑149 (MQ=255)
                                                                            cTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCAcggt                                                                          >  4:325791/1‑149 (MQ=255)
                                                                               aGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCAcggtcgg                                                                       <  2:290809/149‑1 (MQ=255)
                                                                                    aaCGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGt                                                                  <  3:325791/149‑1 (MQ=255)
                                                                                    aaCGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGt                                                                  <  2:520590/149‑1 (MQ=255)
                                                                                      cgacgCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGttt                                                                <  4:615672/149‑1 (MQ=255)
                                                                                       gacgCAATGTGGACACACTTGGAGGATTACTTCTGTATTCTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTtc                                                               >  1:353220/1‑149 (MQ=255)
                                                                                         cgCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTtctc                                                             <  4:600983/149‑1 (MQ=255)
                                                                                           cAATGTGGACGCACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCca                                                           >  1:128605/1‑149 (MQ=255)
                                                                                                tgGACGCAATTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGGAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACagaa                                                      <  2:396592/149‑1 (MQ=255)
                                                                                                tgGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACaaaa                                                      <  2:211201/149‑1 (MQ=255)
                                                                                                 ggACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCTTTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAg                                                                                             <  4:42213/109‑1 (MQ=255)
                                                                                                 ggACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAg                                                                                             <  4:603203/109‑1 (MQ=255)
                                                                                                 ggACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAg                                                                                             >  3:42213/1‑109 (MQ=255)
                                                                                                 ggACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAg                                                                                             >  3:603203/1‑109 (MQ=255)
                                                                                                  gACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACa                                                    <  2:220818/149‑1 (MQ=255)
                                                                                                   acacacTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACag                                                   <  2:10175/149‑1 (MQ=255)
                                                                                                    cacacTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGaa                                                                                                                                         >  1:360807/1‑62 (MQ=255)
                                                                                                    cacacTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGaa                                                                                                                                         <  2:360807/62‑1 (MQ=255)
                                                                                                       acTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGagaa                                               <  2:557433/149‑1 (MQ=255)
                                                                                                       acTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGagaa                                               <  1:515642/149‑1 (MQ=255)
                                                                                                                 ttACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCtgt                                     <  2:517351/149‑1 (MQ=255)
                                                                                                                 ttACTTCTGTATTTTTCAGCAGCCAATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCtgt                                     <  2:186737/149‑1 (MQ=255)
                                                                                                                          tATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCGTGCAgggtcgatatg                                                                   <  2:217590/110‑6 (MQ=255)
                                                                                                                          tATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCGTGCAgggtcgatatg                                                                   >  1:217590/1‑105 (MQ=255)
                                                                                                                               ttCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCa                       <  2:504316/149‑1 (MQ=255)
                                                                                                                                   gcagcCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGAt                   <  1:13105/149‑1 (MQ=255)
                                                                                                                                   gcagcCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGAt                   <  1:19050/149‑1 (MQ=255)
                                                                                                                                   gcagcCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGAt                   <  1:442185/149‑1 (MQ=255)
                                                                                                                                       cccATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGt               >  3:218344/1‑149 (MQ=255)
                                                                                                                                          atgcttcAAGTGTTAACTCTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTcc                                                            >  3:432522/4‑101 (MQ=255)
                                                                                                                                          atgcttcAAATGTTAACTCTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTcc                                                            <  4:432522/98‑1 (MQ=255)
                                                                                                                                           ttcttcAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCggg           <  1:492972/149‑1 (MQ=255)
                                                                                                                                            tcttcAAGAGCTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGCGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGt          >  4:402731/1‑149 (MQ=255)
                                                                                                                                            gcttcAAATGTTAACTCTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCcaca                              <  3:27129/128‑1 (MQ=255)
                                                                                                                                            gcttcAAATGTTAACTCTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCcaca                              >  4:27129/2‑129 (MQ=255)
                                                                                                                                               tcAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGttat       <  4:319632/149‑1 (MQ=255)
                                                                                                                                                 aaGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCggg           <  4:464942/143‑1 (MQ=255)
                                                                                                                                                 aaGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCggg           >  3:464942/1‑143 (MQ=255)
                                                                                                                                                    agTTAACATTTGAAGCATATGCGATCACCTTATTACTTCACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGTTATTAAcc  <  3:402731/149‑1 (MQ=255)
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CCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGTTATTAACC  >  NC_000913/1200867‑1201163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: