Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,201,022 A→T 5.3% M4K (ATG→AAG) ‡ xisE ← e14 prophage; putative excisionase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,201,0220AT 5.3% 272.7 / 5.0 76M4K (ATG→AAG) ‡xisEe14 prophage; putative excisionase
Reads supporting (aligned to +/- strand):  ref base A (27/45);  new base T (2/2);  total (29/47)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

GGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGTTATTAACCCG  >  NC_000913/1200880‑1201165
                                                                                                                                              |                                                                                                                                               
ggCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGaa                                                                                                                                           <  1:228487/149‑1 (MQ=255)
      cTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCatat                                                                                                                                     >  2:515642/1‑149 (MQ=255)
      cTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCatat                                                                                                                                     <  4:581236/149‑1 (MQ=255)
       tCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATg                                                                                                                                    >  1:77131/1‑149 (MQ=255)
       tCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATg                                                                                                                                    >  4:406663/1‑149 (MQ=255)
           gCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGAt                                                                                                                                <  1:199531/149‑1 (MQ=255)
           gCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGAt                                                                                                                                <  4:279460/149‑1 (MQ=255)
                    aTAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatt                                                                                                                       <  1:561470/149‑1 (MQ=255)
                     tAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatta                                                                                                                      >  3:615672/1‑149 (MQ=255)
                     ccaCCGGCCATGAAACAAGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatta                                                                                                                      >  1:503986/3‑149 (MQ=255)
                        ccGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtacta                                                                                                                   <  2:77043/149‑1 (MQ=255)
                        ccGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtacta                                                                                                                   <  1:232491/149‑1 (MQ=255)
                        ccGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtacta                                                                                                                   <  2:77131/149‑1 (MQ=255)
                         cGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtactac                                                                                                                  >  2:82264/1‑149 (MQ=255)
                         cGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtactac                                                                                                                  >  3:319632/1‑149 (MQ=255)
                            ccTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACt                                                                                                               >  4:235067/1‑149 (MQ=255)
                                 tttCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTACCTg                                                                                                          <  1:507107/149‑1 (MQ=255)
                                  ttCATGGCCGGTGGACAAAGTAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGc                                                                                                         <  4:423021/149‑1 (MQ=255)
                                   tCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCt                                                                                                        <  1:176000/149‑1 (MQ=255)
                                    cATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGAt                                                                                                                                <  3:217903/124‑1 (MQ=255)
                                    cATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGAt                                                                                                                                >  4:217903/1‑124 (MQ=255)
                                     aTGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTa                                                                                                      <  3:235067/149‑1 (MQ=255)
                                       ggCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGt                                                                                                    <  3:221969/149‑1 (MQ=255)
                                        gCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGtc                                                                                                   >  3:600983/1‑149 (MQ=255)
                                          cGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGtctc                                                                                                 <  1:82264/149‑1 (MQ=255)
                                           ggTGGATAAAATAAATTCTGCTTAGCATAACGCCGCAATGTGGACACACTTGTAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCGCCTTATTACTACACTAACTGCTTAGTCTCa                                                                                                <  2:298410/149‑1 (MQ=255)
                                           ggTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCa                                                                                                <  1:344218/149‑1 (MQ=255)
                                                  aaaaTAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATc                                                                                         <  2:507987/149‑1 (MQ=255)
                                                     ataaatTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatta                                                                                                                      >  3:433594/1‑117 (MQ=255)
                                                     ataaatTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatta                                                                                                                      <  4:433594/117‑1 (MQ=255)
                                                     ataaatTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGAc                                                                                      >  2:19050/1‑149 (MQ=255)
                                                     ataaatTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGAc                                                                                      >  4:583304/1‑149 (MQ=255)
                                                               cTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCAcggt                                                                            >  4:325791/1‑149 (MQ=255)
                                                               cTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCAcggt                                                                            >  1:504316/1‑149 (MQ=255)
                                                                  aGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCAcggtcgg                                                                         <  2:290809/149‑1 (MQ=255)
                                                                       aaCGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGt                                                                    <  3:325791/149‑1 (MQ=255)
                                                                       aaCGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGt                                                                    <  2:520590/149‑1 (MQ=255)
                                                                         cgacgCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGttt                                                                  <  4:615672/149‑1 (MQ=255)
                                                                          gacgCAATGTGGACACACTTGGAGGATTACTTCTGTATTCTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTtc                                                                 >  1:353220/1‑149 (MQ=255)
                                                                            cgCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTtctc                                                               <  4:600983/149‑1 (MQ=255)
                                                                              cAATGTGGACGCACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCca                                                             >  1:128605/1‑149 (MQ=255)
                                                                                   tgGACGCAATTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGGAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACagaa                                                        <  2:396592/149‑1 (MQ=255)
                                                                                   tgGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACaaaa                                                        <  2:211201/149‑1 (MQ=255)
                                                                                    ggACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCTTTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAg                                                                                               <  4:42213/109‑1 (MQ=255)
                                                                                    ggACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAg                                                                                               >  3:603203/1‑109 (MQ=255)
                                                                                    ggACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAg                                                                                               <  4:603203/109‑1 (MQ=255)
                                                                                    ggACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAg                                                                                               >  3:42213/1‑109 (MQ=255)
                                                                                     gACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACa                                                      <  2:220818/149‑1 (MQ=255)
                                                                                      acacacTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACag                                                     <  2:10175/149‑1 (MQ=255)
                                                                                       cacacTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGaa                                                                                                                                           <  2:360807/62‑1 (MQ=255)
                                                                                       cacacTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGaa                                                                                                                                           >  1:360807/1‑62 (MQ=255)
                                                                                          acTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGagaa                                                 <  2:557433/149‑1 (MQ=255)
                                                                                          acTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGagaa                                                 <  1:515642/149‑1 (MQ=255)
                                                                                                    ttACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCtgt                                       <  2:517351/149‑1 (MQ=255)
                                                                                                    ttACTTCTGTATTTTTCAGCAGCCAATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCtgt                                       <  2:186737/149‑1 (MQ=255)
                                                                                                             tATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCGTGCAgggtcgatatg                                                                     >  1:217590/1‑105 (MQ=255)
                                                                                                             tATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCGTGCAgggtcgatatg                                                                     <  2:217590/110‑6 (MQ=255)
                                                                                                                  ttCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCa                         <  2:504316/149‑1 (MQ=255)
                                                                                                                      gcagcCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGAt                     <  1:13105/149‑1 (MQ=255)
                                                                                                                      gcagcCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGAt                     <  1:19050/149‑1 (MQ=255)
                                                                                                                      gcagcCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGAt                     <  1:442185/149‑1 (MQ=255)
                                                                                                                          cccATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGt                 >  3:218344/1‑149 (MQ=255)
                                                                                                                             atgcttcAAGTGTTAACTCTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTcc                                                              >  3:432522/4‑101 (MQ=255)
                                                                                                                             atgcttcAAATGTTAACTCTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTcc                                                              <  4:432522/98‑1 (MQ=255)
                                                                                                                              ttcttcAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCggg             <  1:492972/149‑1 (MQ=255)
                                                                                                                               tcttcAAGAGCTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGCGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGt            >  4:402731/1‑149 (MQ=255)
                                                                                                                               gcttcAAATGTTAACTCTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCcaca                                >  4:27129/2‑129 (MQ=255)
                                                                                                                               gcttcAAATGTTAACTCTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCcaca                                <  3:27129/128‑1 (MQ=255)
                                                                                                                                  tcAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGttat         <  4:319632/149‑1 (MQ=255)
                                                                                                                                    aaGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCggg             <  4:464942/143‑1 (MQ=255)
                                                                                                                                    aaGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCggg             >  3:464942/1‑143 (MQ=255)
                                                                                                                                       agTTAACATTTGAAGCATATGCGATCACCTTATTACTTCACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGTTATTAAcc    <  3:402731/149‑1 (MQ=255)
                                                                                                                                        gTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGTTATTAAccc   <  2:503986/149‑1 (MQ=255)
                                                                                                                                         ttAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGTTATTAACCCg  >  2:222961/1‑149 (MQ=255)
                                                                                                                                         ttAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGTTATTAACCCg  >  2:436865/1‑149 (MQ=255)
                                                                                                                                         ttAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGTTATTAACCCg  >  2:586675/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                               
GGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCTTAGTCTCAGCATATCGACCCTGCACGGTCGGTTAGTTTCTCCACAAAACAGAGAAGAGCACCTGTGGCCACAGCTATCAGGATGGGTCGGGTTATTAACCCG  >  NC_000913/1200880‑1201165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: