Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,211,400	A→G	7.4%	intergenic (+221/‑13)	mcrA → / → icdC	putative 5‑methylcytosine/5‑hydroxymethylcytosine‑specific restriction nuclease; 5‑methylcytosine DNA binding protein/pseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,211,400	0	A	G	7.4%	168.7 / 4.1	54	intergenic (+221/‑13)	mcrA/icdC	putative 5‑methylcytosine/5‑hydroxymethylcytosine‑specific restriction nuclease; 5‑methylcytosine DNA binding protein/pseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion
Reads supporting (aligned to +/- strand):  ref base A (23/27);  new base G (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

CAAATGTTAACTGCTATTTTTATGCTAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAG  >  NC_000913/1211252‑1211545                                                                                                                                                     |                                                                                                                                                  cAAATGTTAACTGCTATTTTTATGCTAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTa                                                                                                                                                   <  2:513086/149‑1 (MQ=255)  aaaTGTTAACTGCTATTTTTATGCTAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAg                                                                                                                                                  >  4:106595/1‑149 (MQ=255)  aaaTGTTAACTGCTATTTTTATGCTAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAg                                                                                                                                                  >  2:334574/1‑149 (MQ=255)           cTGCTATTTTTATGCTAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATctaac                                                                                                                                         >  4:249817/1‑149 (MQ=255)            tGCTATTTTTATGCTAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATctaact                                                                                                                                        >  1:600633/1‑149 (MQ=255)               tATTTTTATGCTAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATctaactaa                                                                                                                                      >  3:34830/1‑148 (MQ=255)               tATTTTTATGCTAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATctaactaa                                                                                                                                      <  4:34830/148‑1 (MQ=255)                         cTAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCat                                                                                                                           >  1:572975/1‑149 (MQ=255)                          tAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCata                                                                                                                          >  3:99852/1‑149 (MQ=255)                          tAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAACCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCata                                                                                                                          >  2:51877/1‑149 (MQ=255)                                tGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGtt                                                                                                                    <  1:539724/149‑1 (MQ=255)                                  gTTATCAAAACTCAAAAACACATGTTTAT‑ATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGa                                                                                                                 <  1:275342/149‑1 (MQ=255)                                   ttATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGa                                                                                                                 >  4:211702/1‑149 (MQ=255)                                        aaaaCTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGaa                                                                                                            >  3:4761/1‑149 (MQ=255)                                        aaaaCTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGaa                                                                                                            <  1:56213/149‑1 (MQ=255)                                           aCTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATc                                                                                                                                             >  1:163752/1‑113 (MQ=255)                                           aCTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATc                                                                                                                                             <  2:163752/113‑1 (MQ=255)                                            cTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCgg                                                                                                        <  1:127665/149‑1 (MQ=255)                                               aaaaaCACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTg                                                                                                     >  2:450196/1‑149 (MQ=255)                                                  aaCACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcc                                                                                                  <  3:106595/149‑1 (MQ=255)                                                  aaCACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcc                                                                                                  <  1:334574/149‑1 (MQ=255)                                                       aTGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAtt                                                                                             <  3:61484/149‑1 (MQ=255)                                                         gTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGt                                                                                           >  4:232713/1‑149 (MQ=255)                                                             ataATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGa                                                                                                    >  4:179931/1‑136 (MQ=255)                                                             ataATCAATGAGTTATAGAAATGCTAAGGGATAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGa                                                                                                    <  3:179931/136‑1 (MQ=255)                                                              taATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                                                                      <  3:232713/149‑1 (MQ=255)                                                              taATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGAATGAAGCGGCTGACCTGATTGTTACAg                                                                                      <  2:572975/149‑1 (MQ=255)                                                               aaTCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATct                                                                                                                                            >  4:305883/1‑94 (MQ=255)                                                               aaTCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATct                                                                                                                                            <  3:305883/94‑1 (MQ=255)                                                               aaTCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg                                                                                     >  2:47718/1‑149 (MQ=255)                                                                 tCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTa                                                                                                                                                   <  3:92838/85‑1 (MQ=255)                                                                 tCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTa                                                                                                                                                   >  4:92838/1‑85 (MQ=255)                                                                  cAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                                                                  <  3:211702/149‑1 (MQ=255)                                                                      gAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTGGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGaa                                                                              >  1:355401/1‑149 (MQ=255)                                                                      gAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTGGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGaa                                                                              >  1:452430/1‑149 (MQ=255)                                                                      gAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGaa                                                                              >  3:124194/1‑149 (MQ=255)                                                                         ttATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgc                                                                           <  1:450196/149‑1 (MQ=255)                                                                          tataGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                                                          <  3:110204/149‑1 (MQ=255)                                                                              gAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaat                                                                      >  1:296803/1‑149 (MQ=255)                                                                                 aTGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatcaa                                                                   <  4:99852/149‑1 (MQ=255)                                                                                       aGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTg                                                                                                     >  3:18202/1‑109 (MQ=255)                                                                                       aGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTg                                                                                                     <  4:18202/109‑1 (MQ=255)                                                                                               tGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                                                                      <  2:525680/116‑1 (MQ=255)                                                                                               tGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                                                                      >  1:525680/1‑116 (MQ=255)                                                                                                  gTTATATGTAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACAATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACtt                                                  <  4:510418/149‑1 (MQ=255)                                                                                                   ttATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGaa                                                                              >  1:473102/1‑120 (MQ=255)                                                                                                   ttATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGaa                                                                              <  2:473102/120‑1 (MQ=255)                                                                                                      tatGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGa                                              <  2:600633/149‑1 (MQ=255)                                                                                                           aaaTTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTGGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg                                         <  2:452430/149‑1 (MQ=255)                                                                                                           aaaTTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTGGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg                                         <  2:355401/149‑1 (MQ=255)                                                                                                              ttAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAAc                                      <  1:47718/149‑1 (MQ=255)                                                                                                                 gTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc                                   <  4:4761/149‑1 (MQ=255)                                                                                                                  tAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt                                  <  3:593474/149‑1 (MQ=255)                                                                                                                     aaTTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGAt                               <  2:296803/149‑1 (MQ=255)                                                                                                                                 aTGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTaa                   <  1:51877/149‑1 (MQ=255)                                                                                                                                                  tttAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCag  >  3:418260/1‑149 (MQ=255)                                                                                                                                                  tttAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCag  >  3:157415/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CAAATGTTAACTGCTATTTTTATGCTAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAG  >  NC_000913/1211252‑1211545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑