Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,535,876 T→A 8.6% intergenic (‑18/+61) yddE ← / ← narV PhzC‑PhzF family protein/nitrate reductase 2 (NRZ), gamma subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,535,8760TA 8.6% 197.3 / 8.7 58intergenic (‑18/+61)yddE/narVPhzC‑PhzF family protein/nitrate reductase 2 (NRZ), gamma subunit
Reads supporting (aligned to +/- strand):  ref base T (26/27);  new base A (2/3);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.17e-01

CCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAG  >  NC_000913/1535731‑1536024
                                                                                                                                                 |                                                                                                                                                    
ccTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGga                                                                                                                                                   >  4:378506/1‑149 (MQ=255)
      tCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCAGTCACAGGAGAAcgt                                                                                                                                             <  4:528317/149‑3 (MQ=255)
          gggCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCAGTCACAGGAGAAcgttaat                                                                                                                                         <  3:592674/149‑7 (MQ=255)
               aTAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCt                                                                                                                                    <  3:474916/149‑1 (MQ=255)
                   gCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCaa                                                                                                                                <  1:127729/149‑1 (MQ=255)
                   gCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCaa                                                                                                                                >  2:320047/1‑149 (MQ=255)
                   gCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCaa                                                                                                                                <  4:576117/149‑1 (MQ=255)
                   gCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCaa                                                                                                                                >  4:310930/1‑149 (MQ=255)
                    cTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGACGCGAAACGGTTGTGAGGTAAAAGCATCGACGTTGTACACCTGCGGGTTCATTAACGTTCTCCTGTGAATGGAGAACTATTATAGCCTGGAAg                                                                                                                               >  2:19122/1‑149 (MQ=255)
                      gCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCCGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTg                                                                                                                             >  2:153913/1‑149 (MQ=255)
                         tttGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGcc                                                                                                                          >  2:516838/1‑149 (MQ=255)
                              gcTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGgaga                                                                                                                     <  1:19122/149‑1 (MQ=255)
                              gcTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGgaga                                                                                                                     >  2:408930/1‑149 (MQ=255)
                                   gCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGaa                                                                                                                <  3:378506/149‑1 (MQ=255)
                                     tAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCAATAACGGTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAgg                                                                                                              >  2:609836/1‑149 (MQ=255)
                                        gATTATCGGCGGGGAAAACAACACTGGCAGAATTTCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCt                                                                                                           >  2:374700/1‑149 (MQ=255)
                                        gATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCt                                                                                                           <  1:320047/149‑1 (MQ=255)
                                         aTTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTa                                                                                                          >  2:46935/1‑149 (MQ=255)
                                                   gggAAAACAAAACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAgg                                                                                                >  3:38464/1‑149 (MQ=255)
                                                          caacaCCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAAt                                                                                         >  2:603130/1‑149 (MQ=255)
                                                             cacCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTAt                                                                                      >  2:294572/1‑149 (MQ=255)
                                                              acCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATc                                                                                     <  1:153913/149‑1 (MQ=255)
                                                               ccGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACCTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATcg                                                                                    <  4:543544/149‑1 (MQ=255)
                                                                  gCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATcgccg                                                                                 >  4:501342/1‑149 (MQ=255)
                                                                        ttGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGaa                                                                                                                >  4:371232/1‑112 (MQ=255)
                                                                        ttGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGaa                                                                                                                <  3:371232/112‑1 (MQ=255)
                                                                                  aCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGa                                                                 <  3:265532/149‑1 (MQ=255)
                                                                                  aCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGa                                                                 >  2:341299/1‑149 (MQ=255)
                                                                                  aCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGa                                                                 <  3:501342/149‑1 (MQ=255)
                                                                                          gAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATcgacg                                                         >  3:216353/1‑149 (MQ=255)
                                                                                           aGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCGTTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATcgacga                                                        <  1:141490/149‑1 (MQ=255)
                                                                                           aGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTAAAATTATCGCCGCGAACGCACAATTTGATATcgacga                                                        <  1:374700/149‑1 (MQ=255)
                                                                                                aaaGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTaaa                                                   <  4:216353/149‑1 (MQ=255)
                                                                                                   gCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTa                                                <  3:483822/149‑1 (MQ=255)
                                                                                                         aCGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCaaa                                          <  3:544763/149‑1 (MQ=255)
                                                                                                             ggTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAgg                                                                                                >  3:198961/1‑91 (MQ=255)
                                                                                                             ggTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAgg                                                                                                <  4:198961/91‑1 (MQ=255)
                                                                                                             ggTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGgc                                      <  4:321405/149‑1 (MQ=255)
                                                                                                              gTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGaa                                                                                           <  3:238709/95‑1 (MQ=255)
                                                                                                              gTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGaa                                                                                           >  4:238709/1‑95 (MQ=255)
                                                                                                                         gTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCcacaca                          >  1:398421/1‑149 (MQ=255)
                                                                                                                              aTTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCAc                     >  2:73736/1‑149 (MQ=255)
                                                                                                                              aTTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCAc                     >  2:73684/1‑149 (MQ=255)
                                                                                                                               tgatagTTCTCCAGTCACAGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCAcc                    >  1:255465/6‑149 (MQ=255)
                                                                                                                                   agTTCTCCAGTCACAGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAAc                                         <  2:274282/123‑1 (MQ=255)
                                                                                                                                   agTTCTCCAGTCACAGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAAc                                         >  1:274282/2‑124 (MQ=255)
                                                                                                                                     ttCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGata                                                               >  4:211474/1‑100 (MQ=255)
                                                                                                                                     ttCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGata                                                               <  3:211474/100‑1 (MQ=255)
                                                                                                                                     ttCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCgg              <  4:480243/149‑1 (MQ=255)
                                                                                                                                      tctcCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCggg             >  4:247352/1‑149 (MQ=255)
                                                                                                                                      tctcCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCggg             <  1:73736/149‑1 (MQ=255)
                                                                                                                                       ctcCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGTGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCGGGt            <  4:426102/149‑1 (MQ=255)
                                                                                                                                       ctcCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGt            <  1:294572/149‑1 (MQ=255)
                                                                                                                                            gtgACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCGGGTGAACg       >  1:250066/1‑149 (MQ=255)
                                                                                                                                             tgACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCGGGTGAACgg      <  1:516838/149‑1 (MQ=255)
                                                                                                                                              gACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCgg              >  4:105261/1‑140 (MQ=255)
                                                                                                                                              gACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCgg              <  3:105261/140‑1 (MQ=255)
                                                                                                                                               aCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCGGGTGAACGGGa    <  1:402611/149‑1 (MQ=255)
                                                                                                                                                 tGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCGGGTGAACGGGAag  >  1:543401/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAG  >  NC_000913/1535731‑1536024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑