| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,701,108 | A→T | 7.1% | N51I (AAT→ATT) | malY → | PLP‑dependent beta‑cystathionase and maltose regulon regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,701,108 | 0 | A | T | 7.1% | 188.6 / 5.5 | 56 | N51I (AAT→ATT) | malY | PLP‑dependent beta‑cystathionase and maltose regulon regulator |
| Reads supporting (aligned to +/- strand): ref base A (29/23); new base T (2/2); total (31/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01 | |||||||||||
TTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATC > NC_000913/1700960‑1701247 | ttCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGaa > 4:45112/1‑149 (MQ=255)ttCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGaa > 2:54332/1‑149 (MQ=255)ttCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGaa > 2:557355/1‑149 (MQ=255) gATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGATTCa > 3:164353/1‑149 (MQ=255) gATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCa > 4:77052/1‑149 (MQ=255) gATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCa < 2:454100/149‑1 (MQ=255) gATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCa < 3:45112/149‑1 (MQ=255) aTTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAg > 4:144504/1‑149 (MQ=255) tttttCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAgc < 2:181464/149‑1 (MQ=255) ttCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGcc < 3:598737/149‑1 (MQ=255) tCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGATTCAGCGCCt < 4:164353/149‑1 (MQ=255) tCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCt < 3:420255/149‑1 (MQ=255) aaaGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGa > 3:23704/1‑149 (MQ=255) ggTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGc > 3:136933/1‑149 (MQ=255) gTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCa > 3:552215/1‑149 (MQ=255) gATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGt > 3:158248/1‑149 (MQ=255) gTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTAttt > 2:107697/1‑149 (MQ=255) tCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTg < 4:158248/149‑1 (MQ=255) acaTGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATAGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAg > 3:420965/1‑149 (MQ=255) caTGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGc > 4:97394/1‑149 (MQ=255) ggTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGATTCAGCGCCt > 3:245162/1‑121 (MQ=255) ggTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGATTCAGCGCCt < 4:245162/121‑1 (MQ=255) acaGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGaaaa > 4:117611/1‑149 (MQ=255) ggATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATg > 1:274304/1‑149 (MQ=255) gATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGa > 2:468717/1‑149 (MQ=255) ttATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGt < 1:258990/149‑1 (MQ=255) aTGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGttt > 1:361447/1‑149 (MQ=255) gCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTcgc > 2:162162/1‑149 (MQ=255) gACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGaa > 1:409191/1‑86 (MQ=255) gACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGaa < 2:409191/86‑1 (MQ=255) ggCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCa > 1:55188/1‑149 (MQ=255) tgctgACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGt > 3:603268/1‑149 (MQ=255) gACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCATTGGTTTTc < 1:132113/149‑1 (MQ=255) aCCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATc > 4:169949/1‑69 (MQ=255) aCCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATc < 3:169949/69‑1 (MQ=255) ccTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCa < 4:23704/149‑1 (MQ=255) cTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCAc < 1:557355/149‑1 (MQ=255) gTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCAccc < 3:117611/149‑1 (MQ=255) tACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAg > 1:198763/1‑149 (MQ=255) ccGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCa < 4:420965/149‑1 (MQ=255) ccGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCa > 1:127537/1‑149 (MQ=255) ttCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGa > 2:336191/1‑104 (MQ=255) ttCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGa < 1:336191/104‑1 (MQ=255) cACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTaca < 3:97394/149‑1 (MQ=255) cACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTaca > 2:12840/1‑149 (MQ=255) tttCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGaaaaa > 3:502050/1‑94 (MQ=255) tttCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGaaaaa < 4:502050/94‑1 (MQ=255) tCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCAt < 3:562165/149‑1 (MQ=255) cATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGAtt < 1:597465/149‑1 (MQ=255) tGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCATCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAGAACGAt > 1:455936/1‑89 (MQ=255) tGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGAt < 2:455936/89‑1 (MQ=255) ccACTGCCCCCTGCATTATCGAGGCGCAGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACggtg < 2:127537/149‑1 (MQ=255) aCTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACggtggt > 4:457126/1‑149 (MQ=255) ccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGc > 2:24876/1‑149 (MQ=255) cTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGcc > 3:282259/1‑149 (MQ=255) tGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGa > 2:597885/1‑125 (MQ=255) tGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGa < 1:597885/125‑1 (MQ=255) gAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCTATTCTCAGACGGTGGTGTATGGCCCTTCTGTCAt > 4:527840/1‑149 (MQ=255) aGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATc < 3:457126/149‑1 (MQ=255) tGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATgg < 1:172435/130‑1 (MQ=255) tGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATgg > 2:172435/1‑130 (MQ=255) | TTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATC > NC_000913/1700960‑1701247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |