| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,890,943 | T→C | 100% | T109A (ACC→GCC) | tsaB ← | tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; binding partner and protease for TsaD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,890,943 | 0 | T | C | 96.9% | 109.3 / ‑3.8 | 32 | T109A (ACC→GCC) | tsaB | tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; binding partner and protease for TsaD |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (13/18); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGTTGCATTCGTTCATGGACGATTTCGGGTTTGAGTACGGCTTCGGTTTCTTCACCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTTGCGCGATGCCAATACCAATGCGCACGCCAGTAAAGCTACCGGGGCCGCGCCC > NC_000913/1890803‑1891084 | cTGTTGCATTCGTTCATGGACGATTTCGGGTTTGAGTACGGCTTCGGTTTCTTCACCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGt > 3:315326/1‑149 (MQ=255) ttCGTTCATGGACGATTTCGGGTTTGAGTACGGCTTCGGTTTCTTCACCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTgcgc > 3:619096/1‑149 (MQ=255) tGGACGATTTCGGGTTTGAGTACGGCTTCGGTTTCTTCACCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGcccc < 2:160461/149‑1 (MQ=255) tttCGGGTTTGAGTACGGCTTCGGTTTCTTCACCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGcc > 2:398624/1‑149 (MQ=255) ttCGGGTTTGAGTACGGCTTCGGTTTCTTCACCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCa > 4:492013/1‑149 (MQ=255) ttGAGTACGGCTTCGGTTTCTTCACCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCAt < 3:231396/149‑1 (MQ=255) gCTTCGGTTTCTTCACCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGga > 1:336664/1‑149 (MQ=255) ttCGGTTTCTTCACCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGgaga > 2:417445/1‑149 (MQ=255) cttcACCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAatc > 4:54680/1‑149 (MQ=255) cACCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGCCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAatcatc < 3:398746/149‑1 (MQ=255) aCCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCg < 4:392165/149‑1 (MQ=255) aCCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCg < 1:500364/149‑1 (MQ=255) ccGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCgg < 3:339054/149‑1 (MQ=255) tGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTaa < 4:19161/149‑1 (MQ=255) tACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCcgcg < 2:375276/149‑1 (MQ=255) ttttCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAg > 1:315394/1‑149 (MQ=255) catcaCGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCCTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGcc < 4:121931/149‑1 (MQ=255) tcaCGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTa > 3:23552/1‑106 (MQ=255) tcaCGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTa < 4:23552/106‑1 (MQ=255) gCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCt > 2:486744/1‑149 (MQ=255) gCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCt < 1:486744/149‑1 (MQ=255) cTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCtt > 2:298542/1‑149 (MQ=255) gATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTTgc < 1:417445/149‑1 (MQ=255) cGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTTGCGCGATg < 1:95889/149‑1 (MQ=255) cGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCACCAAGCGCCAGCCCTTGCGCGATg < 1:530217/149‑1 (MQ=255) gCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTTGCGCGATGcc > 4:20225/1‑149 (MQ=255) cccAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTTGCGCGATGCCa < 1:398624/149‑1 (MQ=255) aGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTTGCGCGATGCCAATa < 3:54680/149‑1 (MQ=255) aGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTTGCGCGATGCCAATa < 2:315394/149‑1 (MQ=255) ttCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTTGCGCGATGCCAATACCAATgc > 2:300017/1‑149 (MQ=255) tGCCAGCACGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTTGCGCGATGCCAATACCAATGCGCACGCCAGTAAAGCTACCGGGGc > 4:451633/1‑149 (MQ=255) aCGCGGGCTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTTGCGCGATGCCAATACCAATGCGCACGCCAGTAAAGCTACCGGGGCCGCGccc < 2:37501/149‑1 (MQ=255) | CTGTTGCATTCGTTCATGGACGATTTCGGGTTTGAGTACGGCTTCGGTTTCTTCACCGTGCCAGATACCGTTTTCATCACGCTGATATTCGGCCCAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTTGCGCGATGCCAATACCAATGCGCACGCCAGTAAAGCTACCGGGGCCGCGCCC > NC_000913/1890803‑1891084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |