Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,913,763 C→T 6.1% V352I (GTC→ATC)  prc ← carboxy‑terminal protease for penicillin‑binding protein 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,913,7630CT 6.1% 211.0 / 3.3 65V352I (GTC→ATC) prccarboxy‑terminal protease for penicillin‑binding protein 3
Reads supporting (aligned to +/- strand):  ref base C (25/36);  new base T (2/2);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

GTTAACGCCCCACCGCCATTGCTACGCAGGTCGATGATGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGG  >  NC_000913/1913615‑1913903
                                                                                                                                                    |                                                                                                                                            
gTTAACGCCCCACCGCCATTGCTACGCAGGTCGATGATGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGAc                                                                                                                                              >  4:444045/1‑149 (MQ=255)
 ttAACGCCCCACCGCCATTGCTACGCAGGTCGATGATGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACg                                                                                                                                             >  4:396439/1‑149 (MQ=255)
      gCCCCACCGCCATTGCTACGCAGGTCGATGATGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCtt                                                                                                                                        <  2:582554/149‑1 (MQ=255)
         ccACCGCCATTGCTACGCAGGTCGATGATGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCAc                                                                                                                                     >  3:9772/1‑149 (MQ=255)
                     cTACGCAGGTCGATGATGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAAc                                                                                                                         >  3:151220/1‑149 (MQ=255)
                     cTACGCAGGTCGATGATGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAAc                                                                                                                         <  2:109539/149‑1 (MQ=255)
                        cGCAGGTCGATGATGACGCTACTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACcgc                                                                                                                      <  3:393159/149‑1 (MQ=255)
                           aGGTCGATGATGACGCTGCTGCCATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACcgcgcg                                                                                                                   >  1:371671/1‑149 (MQ=255)
                           aGGTCGATGATGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACcgcgcg                                                                                                                   <  4:27071/149‑1 (MQ=255)
                            ggTCGATGATGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCgg                                                                                                                  >  2:451711/1‑149 (MQ=255)
                              tCGATGATGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTc                                                                                                                >  4:546129/1‑149 (MQ=255)
                                gatgatGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTGTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCtg                                                                                                              >  3:53990/1‑148 (MQ=255)
                                gatgatGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCtt                                                                                                              >  1:513338/1‑149 (MQ=255)
                                   gatgaCGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCga                                                                                                           <  1:514763/149‑1 (MQ=255)
                                            gctgACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACGTCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTTGAGACGAATAc                                                                                                  <  1:326698/149‑1 (MQ=255)
                                              tgACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGt                                                                                                >  1:196511/1‑149 (MQ=255)
                                                  aTTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTCTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCAc                                                                                            >  3:381154/1‑149 (MQ=255)
                                                   ttCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGAc                                                                                                                                 <  4:586141/111‑1 (MQ=255)
                                                   ttCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGAc                                                                                                                                 >  3:586141/1‑111 (MQ=255)
                                                       gTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACg                                                                                                                                             >  1:58704/1‑95 (MQ=255)
                                                       gTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACg                                                                                                                                             <  2:58704/95‑1 (MQ=255)
                                                       gTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTc                                                                                       >  1:584780/1‑149 (MQ=255)
                                                                   tCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTa                                                                           >  3:405340/1‑149 (MQ=255)
                                                                          ttGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGATGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCt                                                                    >  1:563839/1‑149 (MQ=255)
                                                                          ttGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCt                                                                    <  1:37715/149‑1 (MQ=255)
                                                                           tGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCtt                                                                   <  4:504086/149‑1 (MQ=255)
                                                                           tGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTACACGGGTCAACGTTACAGTACGGGTcat                                                                   <  2:584780/149‑3 (MQ=255)
                                                                             cACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTgg                                                                 <  2:391532/149‑1 (MQ=255)
                                                                             cACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTgg                                                                 <  1:507996/149‑1 (MQ=255)
                                                                               cTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTc                                                               <  4:405340/149‑1 (MQ=255)
                                                                               cTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTc                                                               <  2:337510/149‑1 (MQ=255)
                                                                                tttGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTcc                                                              >  4:273712/1‑149 (MQ=255)
                                                                                 ttGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTccc                                                             <  3:444045/149‑1 (MQ=255)
                                                                                 ttGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTccc                                                             <  2:323521/149‑1 (MQ=255)
                                                                                   gACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCtt                                                           <  4:9772/149‑1 (MQ=255)
                                                                                   gACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCtt                                                           <  3:348815/149‑1 (MQ=255)
                                                                                       tCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTAcc                                                       <  4:340827/149‑1 (MQ=255)
                                                                                          tCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACcagc                                                    >  3:170884/1‑149 (MQ=255)
                                                                                                   cccACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACTGTCTTCACCGAAATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGATCAACGTTACAGTACGGGTCTTGGTCCCTTTACCTGCTGGTaaagg                                           <  4:381154/149‑3 (MQ=255)
                                                                                                   cccACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAAt                                           <  2:370287/149‑1 (MQ=255)
                                                                                                    ccACATAGATGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGATGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGGCCCTTTACCAGCAGGTAAAAtt                                          >  1:535530/1‑149 (MQ=255)
                                                                                                    ccACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATa                                                                                                   <  3:355667/92‑1 (MQ=255)
                                                                                                    ccACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATa                                                                                                   >  4:355667/1‑92 (MQ=255)
                                                                                                                     atatCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACt                         <  1:451711/149‑1 (MQ=255)
                                                                                                                      tatCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTg                        <  2:313315/149‑1 (MQ=255)
                                                                                                                       atCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGc                       <  1:43503/149‑1 (MQ=255)
                                                                                                                        tCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGcc                      <  2:456888/149‑1 (MQ=255)
                                                                                                                          cAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCt                    >  4:602315/1‑149 (MQ=255)
                                                                                                                           aGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTc                                                                                                             >  3:464021/1‑59 (MQ=255)
                                                                                                                           aGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTc                                                                                                             <  4:464021/59‑1 (MQ=255)
                                                                                                                            gCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTc                  >  2:584189/1‑149 (MQ=255)
                                                                                                                             cACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCg                 <  2:371671/149‑1 (MQ=255)
                                                                                                                                gCCGACTTTCTCTTTACCGATGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGcc              <  2:563839/149‑1 (MQ=255)
                                                                                                                                  cGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCt            >  2:520090/1‑149 (MQ=255)
                                                                                                                                    aCTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCttt          <  3:546129/149‑1 (MQ=255)
                                                                                                                                      tttCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTttaa        <  2:513338/149‑1 (MQ=255)
                                                                                                                                      tttCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTttaa        >  3:397470/1‑149 (MQ=255)
                                                                                                                                        tctcTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTttaatt      >  3:373738/1‑149 (MQ=255)
                                                                                                                                          tctTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTttaattaa    <  4:151220/149‑1 (MQ=255)
                                                                                                                                          tctTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTttaattaa    >  1:128524/1‑149 (MQ=255)
                                                                                                                                           ctTTACCGATGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTAACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAg   <  2:535530/149‑1 (MQ=255)
                                                                                                                                           ctTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAg   <  4:397470/149‑1 (MQ=255)
                                                                                                                                           ctTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAg   <  4:134081/149‑1 (MQ=255)
                                                                                                                                            tttACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAgg  <  4:53990/149‑1 (MQ=255)
                                                                                                                                               aCCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGcc              >  4:156048/1‑134 (MQ=255)
                                                                                                                                               aCCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGcc              <  3:156048/134‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GTTAACGCCCCACCGCCATTGCTACGCAGGTCGATGATGACGCTGCTGACATTCTGTTTTTCCAGTTTCTGCAGTTGCACTTTGACATCGTCTGTCAAACCCACATAGAAGCCCGGAATATCCAGCACGCCGACTTTCTCTTTACCGACGGTCTTCACCGACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGG  >  NC_000913/1913615‑1913903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑