Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,009,733 C→T 11.9% intergenic (+21/‑88) yedF → / → yedK putative TusA family sulfurtransferase/DUF159 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,009,7330CT11.9% 116.1 / 7.6 42intergenic (+21/‑88)yedF/yedKputative TusA family sulfurtransferase/DUF159 family protein
Reads supporting (aligned to +/- strand):  ref base C (21/16);  new base T (3/2);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

CTGGAAGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAG  >  NC_000913/2009587‑2009881
                                                                                                                                                  |                                                                                                                                                    
cTGGAAGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCca                                                                                                                                                    >  3:598331/1‑149 (MQ=255)
             gCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATc                                                                                                                                       >  1:494195/1‑149 (MQ=255)
                 cTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTa                                                                                                                                   <  3:343445/149‑1 (MQ=255)
                      cGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCttt                                                                                                                              <  1:52564/149‑1 (MQ=255)
                       gCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTg                                                                                                                             >  2:362003/1‑149 (MQ=255)
                        cAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGt                                                                                                                            <  1:362003/149‑1 (MQ=255)
                           tCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATc                                                                                                                         <  2:306231/149‑1 (MQ=255)
                           tCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATc                                                                                                                         >  1:541053/1‑149 (MQ=255)
                                 aataatATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTAt                                                                                                                          <  1:183054/142‑1 (MQ=255)
                                 aataatATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTAt                                                                                                                          >  2:183054/1‑142 (MQ=255)
                                  ataataTTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGa                                                                                                                  <  1:121336/149‑1 (MQ=255)
                                                     ccgTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTcc                                                                                               >  2:619499/2‑149 (MQ=255)
                                                             aCGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCttt                                                                                       <  3:123950/149‑1 (MQ=255)
                                                                 cTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAg                                                                                   <  3:372485/149‑1 (MQ=255)
                                                                       ggTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGAt                                                                             >  3:284371/1‑149 (MQ=255)
                                                                           cTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCa                                                                         <  1:605732/149‑1 (MQ=255)
                                                                              gATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCagga                                                                      <  2:494195/149‑1 (MQ=255)
                                                                                  ttCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGtt                                                                  >  1:311158/1‑149 (MQ=255)
                                                                                   tCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGttt                                                                 >  1:488377/1‑149 (MQ=255)
                                                                                   tCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGttt                                                                 >  1:3718/1‑149 (MQ=255)
                                                                                         aGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTAtgt                                                           <  4:284371/149‑1 (MQ=255)
                                                                                         aGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTAtgt                                                           >  1:173094/1‑149 (MQ=255)
                                                                                                  gACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCt                                                  >  1:15947/1‑149 (MQ=255)
                                                                                                    ccATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCttt                                                <  4:598331/149‑1 (MQ=255)
                                                                                                       ttCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCCGGAGGTTTCTATGTGTGGACGCTTTGcc                                             >  1:83040/1‑149 (MQ=255)
                                                                                                       ttCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGcc                                             <  1:315271/149‑1 (MQ=255)
                                                                                                                  aTTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAAc                                  >  1:550184/1‑149 (MQ=255)
                                                                                                                      aGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGt                              >  1:187937/1‑149 (MQ=255)
                                                                                                                       gAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTg                             <  2:550184/149‑1 (MQ=255)
                                                                                                                        aaGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGa                            >  2:126069/1‑149 (MQ=255)
                                                                                                                         aGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGaa                           <  1:619499/149‑1 (MQ=255)
                                                                                                                            aaTCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGAt                        <  4:1381/149‑1 (MQ=255)
                                                                                                                                 gatgttCCCGCTGTGGGTCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCtttt                                                                                      >  2:606669/5‑82 (MQ=37)
                                                                                                                                 gatgttCCCGCTGTGGGTCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCtttt                                                                                      <  1:606669/78‑1 (MQ=37)
                                                                                                                                 cTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCt                   >  1:131264/1‑149 (MQ=255)
                                                                                                                                 cTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCt                   >  1:326292/1‑149 (MQ=255)
                                                                                                                                 cTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCt                   >  2:390507/1‑149 (MQ=255)
                                                                                                                                       cccGCTGTGGGTCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTAtgt                                                           <  2:74139/103‑1 (MQ=255)
                                                                                                                                       cccGCTGTGGGTCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTAtgt                                                           >  1:74139/1‑103 (MQ=255)
                                                                                                                                       cccGCTGTGGGTCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCt             >  4:500204/1‑149 (MQ=255)
                                                                                                                                        ccGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCtt            >  4:349261/1‑149 (MQ=255)
                                                                                                                                                aCCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGa    >  1:45855/1‑149 (MQ=255)
                                                                                                                                                  ccACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAg  >  2:593577/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTGGAAGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAG  >  NC_000913/2009587‑2009881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑