Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,117,893 T→C 6.6% N171S (AAC→AGC)  wcaK ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,117,8930TC 6.6% 193.2 / 3.2 60N171S (AAC→AGC) wcaKcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base T (27/29);  new base C (2/2);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

GTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAC  >  NC_000913/2117754‑2118035
                                                                                                                                           |                                                                                                                                              
gTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGtt                                                                                                                                       >  1:46916/1‑149 (MQ=255)
 tcttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTg                                                                                                                                      >  4:552391/1‑149 (MQ=255)
  cttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTgg                                                                                                                                     <  4:196057/149‑1 (MQ=255)
   ttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGt                                                                                                                                    >  3:402475/1‑149 (MQ=255)
   ttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGt                                                                                                                                    >  3:152330/1‑149 (MQ=255)
   ttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGCTCGCCAGTTGGt                                                                                                                                    >  3:558054/1‑149 (MQ=255)
     ctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGCTCGCCAGTTGGTTa                                                                                                                                  >  3:204951/1‑149 (MQ=255)
       gtgtgGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGAAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTaaa                                                                                                                                <  3:584198/149‑1 (MQ=255)
              gATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCa                                                                                                                         >  2:468735/1‑149 (MQ=255)
               aTCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAg                                                                                                                                         <  1:486426/132‑1 (MQ=255)
               aTCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAg                                                                                                                                         >  2:486426/1‑132 (MQ=255)
                  gACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCt                                                                                                                     >  1:193645/1‑149 (MQ=255)
                  gACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCt                                                                                                                     <  3:180072/149‑1 (MQ=255)
                  gACCAGCCACGAGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTCCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCt                                                                                                                     >  1:507988/1‑149 (MQ=255)
                          aCGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACggg                                                                                                             <  3:118572/149‑1 (MQ=255)
                                    gACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACtgt                                                                                                   >  3:128706/1‑149 (MQ=255)
                                        ccATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGAcc                                                                                               >  2:265502/1‑149 (MQ=255)
                                        ccATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGAcc                                                                                               >  4:249288/1‑149 (MQ=255)
                                                 aCTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATaaa                                                                                      <  4:473151/149‑1 (MQ=255)
                                                    tttGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAg                                                                                   <  1:337706/149‑1 (MQ=255)
                                                     ttGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGc                                                                                  <  1:202188/149‑1 (MQ=255)
                                                         ggtggtGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCt                                                                              <  4:428421/149‑1 (MQ=255)
                                                           tggtgATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCttt                                                                            >  2:100100/1‑149 (MQ=255)
                                                              tgATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTc                                                                         <  2:333957/149‑1 (MQ=255)
                                                                      ctgcGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATaaa                                                                 >  2:221116/1‑149 (MQ=255)
                                                                       tgcGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAAc                                                                >  3:441322/1‑149 (MQ=255)
                                                                         cGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGt                                                              <  2:390645/149‑1 (MQ=255)
                                                                         cGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGt                                                              <  4:347099/149‑1 (MQ=255)
                                                                          gTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTg                                                             >  4:80124/1‑149 (MQ=255)
                                                                           tttCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATc                                                                                           >  4:439697/1‑118 (MQ=255)
                                                                           tttCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATc                                                                                           <  3:439697/118‑1 (MQ=255)
                                                                              catcaGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCaaa                                                         <  1:221116/149‑1 (MQ=255)
                                                                                tcaGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGc                                                                                  >  2:591669/1‑122 (MQ=255)
                                                                                tcaGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGc                                                                                  <  1:591669/122‑1 (MQ=255)
                                                                                 caGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTg                                                      <  1:401952/149‑1 (MQ=255)
                                                                                  aGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGCCCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGc                                                     <  4:240157/149‑1 (MQ=255)
                                                                                    aTCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCAt                                                   <  2:507988/149‑1 (MQ=255)
                                                                                    aTCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCAt                                                   <  2:46916/149‑1 (MQ=255)
                                                                                       aaaGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGtt                                                >  2:481981/1‑149 (MQ=255)
                                                                                        aaGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGCTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTc                                               <  4:204951/149‑1 (MQ=255)
                                                                                          gCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATc                                                                                           >  3:425064/1‑103 (MQ=255)
                                                                                          gCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATc                                                                                           <  4:425064/103‑1 (MQ=255)
                                                                                           cTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCaaa                                            <  1:348118/149‑1 (MQ=255)
                                                                                             gACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACt                                          >  3:290150/1‑149 (MQ=255)
                                                                                              aCCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTg                                         >  3:448406/1‑149 (MQ=255)
                                                                                                 gATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCgg                                      >  4:300631/1‑149 (MQ=255)
                                                                                                  aTTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGc                                     >  1:359326/1‑149 (MQ=255)
                                                                                                            gATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATaga                           <  3:248114/149‑1 (MQ=255)
                                                                                                              tCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAgaga                         <  4:128706/149‑1 (MQ=255)
                                                                                                               cAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGAt                        <  2:193645/149‑1 (MQ=255)
                                                                                                                aGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATc                       <  1:100100/149‑1 (MQ=255)
                                                                                                                aGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGAGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATc                       <  2:341510/149‑1 (MQ=255)
                                                                                                                            tGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGa           >  4:492494/1‑149 (MQ=255)
                                                                                                                             gACCAAAAACGTAGCTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGAt          <  4:558054/149‑1 (MQ=255)
                                                                                                                                cAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATccg       >  1:606728/1‑149 (MQ=255)
                                                                                                                                cAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATccg       >  2:477706/1‑149 (MQ=255)
                                                                                                                                 aaaaaCGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATccgc      <  3:300631/149‑1 (MQ=255)
                                                                                                                                 aaaaaCGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATccgc      <  1:468735/149‑1 (MQ=255)
                                                                                                                                     aCGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAc  <  3:552391/149‑1 (MQ=255)
                                                                                                                                     aCGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAc  >  3:517593/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                              
GTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAC  >  NC_000913/2117754‑2118035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑