Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,117,893 | T→C | 6.6% | N171S (AAC→AGC) | wcaK ← | colanic acid biosynthesis protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,117,893 | 0 | T | C | 6.6% | 193.2 / 3.2 | 60 | N171S (AAC→AGC) | wcaK | colanic acid biosynthesis protein |
Reads supporting (aligned to +/- strand): ref base T (27/29); new base C (2/2); total (29/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 |
GTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAC > NC_000913/2117754‑2118035 | gTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGtt > 1:46916/1‑149 (MQ=255) tcttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTg > 4:552391/1‑149 (MQ=255) cttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTgg < 4:196057/149‑1 (MQ=255) ttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGt > 3:402475/1‑149 (MQ=255) ttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGt > 3:152330/1‑149 (MQ=255) ttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGCTCGCCAGTTGGt > 3:558054/1‑149 (MQ=255) ctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGCTCGCCAGTTGGTTa > 3:204951/1‑149 (MQ=255) gtgtgGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGAAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTaaa < 3:584198/149‑1 (MQ=255) gATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCa > 2:468735/1‑149 (MQ=255) aTCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAg < 1:486426/132‑1 (MQ=255) aTCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAg > 2:486426/1‑132 (MQ=255) gACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCt > 1:193645/1‑149 (MQ=255) gACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCt < 3:180072/149‑1 (MQ=255) gACCAGCCACGAGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTCCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCt > 1:507988/1‑149 (MQ=255) aCGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACggg < 3:118572/149‑1 (MQ=255) gACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACtgt > 3:128706/1‑149 (MQ=255) ccATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGAcc > 2:265502/1‑149 (MQ=255) ccATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGAcc > 4:249288/1‑149 (MQ=255) aCTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATaaa < 4:473151/149‑1 (MQ=255) tttGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAg < 1:337706/149‑1 (MQ=255) ttGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGc < 1:202188/149‑1 (MQ=255) ggtggtGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCt < 4:428421/149‑1 (MQ=255) tggtgATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCttt > 2:100100/1‑149 (MQ=255) tgATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTc < 2:333957/149‑1 (MQ=255) ctgcGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATaaa > 2:221116/1‑149 (MQ=255) tgcGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAAc > 3:441322/1‑149 (MQ=255) cGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGt < 2:390645/149‑1 (MQ=255) cGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGt < 4:347099/149‑1 (MQ=255) gTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTg > 4:80124/1‑149 (MQ=255) tttCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATc > 4:439697/1‑118 (MQ=255) tttCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATc < 3:439697/118‑1 (MQ=255) catcaGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCaaa < 1:221116/149‑1 (MQ=255) tcaGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGc > 2:591669/1‑122 (MQ=255) tcaGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGc < 1:591669/122‑1 (MQ=255) caGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTg < 1:401952/149‑1 (MQ=255) aGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGCCCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGc < 4:240157/149‑1 (MQ=255) aTCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCAt < 2:507988/149‑1 (MQ=255) aTCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCAt < 2:46916/149‑1 (MQ=255) aaaGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGtt > 2:481981/1‑149 (MQ=255) aaGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGCTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTc < 4:204951/149‑1 (MQ=255) gCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATc > 3:425064/1‑103 (MQ=255) gCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATc < 4:425064/103‑1 (MQ=255) cTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCaaa < 1:348118/149‑1 (MQ=255) gACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACt > 3:290150/1‑149 (MQ=255) aCCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTg > 3:448406/1‑149 (MQ=255) gATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCgg > 4:300631/1‑149 (MQ=255) aTTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGc > 1:359326/1‑149 (MQ=255) gATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATaga < 3:248114/149‑1 (MQ=255) tCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAgaga < 4:128706/149‑1 (MQ=255) cAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGAt < 2:193645/149‑1 (MQ=255) aGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATc < 1:100100/149‑1 (MQ=255) aGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGAGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATc < 2:341510/149‑1 (MQ=255) tGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGa > 4:492494/1‑149 (MQ=255) gACCAAAAACGTAGCTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGAt < 4:558054/149‑1 (MQ=255) cAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATccg > 1:606728/1‑149 (MQ=255) cAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATccg > 2:477706/1‑149 (MQ=255) aaaaaCGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATccgc < 3:300631/149‑1 (MQ=255) aaaaaCGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATccgc < 1:468735/149‑1 (MQ=255) aCGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAc < 3:552391/149‑1 (MQ=255) aCGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAc > 3:517593/1‑149 (MQ=255) | GTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAC > NC_000913/2117754‑2118035 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |