| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,180,043 | C→T | 100% | T408M (ACG→ATG) | yegT → | nucleoside transporter, low affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,180,043 | 0 | C | T | 96.6% | 204.2 / ‑2.1 | 59 | T408M (ACG→ATG) | yegT | nucleoside transporter, low affinity |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (24/33); total (25/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATGATGGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCCGCGTTAAA > NC_000913/2179897‑2180190 | gatgatGGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATgg < 4:258944/149‑1 (MQ=255) atgatgGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGc < 4:585933/149‑1 (MQ=255) gatgGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTa < 2:465015/149‑1 (MQ=255) tgGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATc > 3:150075/1‑149 (MQ=255) ggAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTACGGCTATCa > 4:600674/1‑149 (MQ=255) gATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCg > 2:24818/1‑149 (MQ=255) gCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATcg < 2:598668/149‑1 (MQ=255) gCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATcg > 2:363702/1‑149 (MQ=255) tATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGa < 2:193121/149‑1 (MQ=255) tCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGata > 2:157377/1‑149 (MQ=255) ggTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGa > 1:187144/1‑149 (MQ=255) cGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAg < 4:82875/149‑1 (MQ=255) cGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAg < 2:352979/149‑1 (MQ=255) actgactTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAgggg > 3:550431/1‑149 (MQ=255) actgactTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAgggg > 2:585694/1‑149 (MQ=255) actgactTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAgggg < 3:87763/149‑1 (MQ=255) tgactTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGaa < 2:515674/149‑1 (MQ=255) tgactTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGaa < 3:457740/149‑1 (MQ=255) tgactTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGaa > 2:83305/1‑149 (MQ=255) tttCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTa > 4:126892/1‑149 (MQ=255) tttCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTa > 1:610342/1‑149 (MQ=255) tCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTaaa < 3:574282/149‑1 (MQ=255) aCTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGa > 3:187689/1‑149 (MQ=255) cTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGTTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGTCTATCAAGGTCGATGAACGCGATATTGCGTTGACACAAGGGGCAGTTAAATGaa < 2:236441/149‑1 (MQ=255) cTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGaa < 2:401660/149‑1 (MQ=255) ggTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGaaaa < 2:610342/149‑1 (MQ=255) gtgGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAAt < 4:465620/134‑1 (MQ=255) gtgGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAAt > 3:465620/1‑134 (MQ=255) gACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCgat < 2:343532/94‑1 (MQ=255) gACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCgat > 1:343532/1‑94 (MQ=255) cTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCg < 1:102820/149‑1 (MQ=255) gcgGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCtt > 2:227410/1‑149 (MQ=255) gTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTAt < 1:343308/149‑1 (MQ=255) tgatgaTTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATg < 1:227410/149‑1 (MQ=255) tgatgaTTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATg < 2:23476/149‑1 (MQ=255) gatgatTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCTATAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGTAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTAggg < 2:257856/149‑1 (MQ=255) ttGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAGGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAgg > 4:61153/1‑149 (MQ=255) cATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGtt > 4:472018/1‑149 (MQ=255) ttATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAg < 1:24818/149‑1 (MQ=255) aTCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAggg < 1:83305/149‑1 (MQ=255) tGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATTGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTGTATGGGCAGGCGTTAGGGGATGCGa < 4:521214/149‑1 (MQ=255) cTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATg < 2:214858/149‑1 (MQ=255) ttCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTAt > 4:369722/1‑106 (MQ=255) ttCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTAt < 1:9204/106‑1 (MQ=255) ttCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTAt > 2:9204/1‑106 (MQ=255) ttCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTAt < 3:369722/106‑1 (MQ=255) cATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGAt < 3:541774/149‑1 (MQ=255) aTGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATg > 2:434624/1‑149 (MQ=255) aTTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGaa < 2:8133/88‑1 (MQ=255) aTTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGaa > 1:8133/1‑88 (MQ=255) tttCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGa < 4:187689/149‑1 (MQ=255) tttCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGa < 2:381300/149‑1 (MQ=255) ttCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAg < 1:363702/149‑1 (MQ=255) cgcgAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCtt < 2:236711/149‑1 (MQ=255) gcgAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCttt > 1:274025/1‑149 (MQ=255) gAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCcgc > 2:489678/1‑149 (MQ=255) gAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCcgc > 4:544199/1‑149 (MQ=255) aTTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCCGCGtt > 2:57187/1‑149 (MQ=255) aCGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGAAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTATTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCCGCGTTaaa < 3:600674/149‑1 (MQ=255) | GATGATGGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCCGCGTTAAA > NC_000913/2179897‑2180190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |