Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,400,684 T→A 5.4% Q291L (CAG→CTG)  nuoF ← NADH:ubiquinone oxidoreductase, chain F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,400,6840TA 5.4% 258.7 / 3.9 74Q291L (CAG→CTG) nuoFNADH:ubiquinone oxidoreductase, chain F
Reads supporting (aligned to +/- strand):  ref base T (37/33);  new base A (2/2);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

CCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCTTTAC  >  NC_000913/2400541‑2400831
                                                                                                                                               |                                                                                                                                                   
ccAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAg                                                                                                                                                <  2:361682/149‑1 (MQ=255)
ccAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAg                                                                                                                                                <  4:575775/149‑1 (MQ=255)
    cGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCAGCCAGGcgg                                                                                                                                            <  4:585132/149‑3 (MQ=255)
        acCATGTTGGTCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTaaa                                                                                                                                        <  1:234081/149‑1 (MQ=255)
        acCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTaaa                                                                                                                                        <  3:381533/149‑1 (MQ=255)
         ccATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAt                                                                                                                                       <  1:31448/149‑1 (MQ=255)
            tGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTc                                                                                                                                    >  2:611527/1‑149 (MQ=255)
            tGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTc                                                                                                                                    >  2:612790/1‑149 (MQ=255)
            tGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTc                                                                                                                                    <  1:612790/149‑1 (MQ=255)
                gATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGAc                                                                                                                                <  3:312518/149‑1 (MQ=255)
                 aTCGCATGATCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGAcc                                                                                                                               >  4:284062/1‑149 (MQ=255)
                   ctcATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCAt                                                                                                                             >  2:574326/1‑149 (MQ=255)
                      aTGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATATTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCAc                                                                                                                          >  1:515063/1‑149 (MQ=255)
                      aTGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCAc                                                                                                                          >  4:496587/1‑149 (MQ=255)
                      aTGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCAc                                                                                                                          >  2:92923/1‑149 (MQ=255)
                          tCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCAGCCAGGcg                                                                                                                                             <  1:434111/126‑2 (MQ=255)
                          tCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCAGCCAGGcg                                                                                                                                             >  2:434111/1‑125 (MQ=255)
                              ccGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATacca                                                                                                                  >  4:197420/1‑149 (MQ=255)
                               cGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATaccac                                                                                                                 <  3:496587/149‑1 (MQ=255)
                                   aTCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGc                                                                                                             <  2:465322/149‑1 (MQ=255)
                                              cGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGa                                                                                                  <  1:191759/149‑1 (MQ=255)
                                               gTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAg                                                                                                 >  3:273377/1‑149 (MQ=255)
                                                 aCCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGa                                                                                               <  3:196326/149‑1 (MQ=255)
                                                  cccAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGAt                                                                                              <  4:404396/149‑1 (MQ=255)
                                                    cAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGAtct                                                                                            >  3:60149/1‑149 (MQ=255)
                                                     aGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGAtctc                                                                                           >  3:203567/1‑149 (MQ=255)
                                                              cccGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggt                                                                                  >  2:4266/1‑149 (MQ=255)
                                                              cccGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggt                                                                                  <  2:514746/149‑1 (MQ=255)
                                                               ccGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAttt                                                                                                                                     <  2:429296/97‑1 (MQ=255)
                                                               ccGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAttt                                                                                                                                     >  1:429296/1‑97 (MQ=255)
                                                               ccGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtg                                                                                 >  4:232409/1‑149 (MQ=255)
                                                               ccGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtg                                                                                 >  3:361123/1‑149 (MQ=255)
                                                               ccGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtg                                                                                 >  4:66302/1‑149 (MQ=255)
                                                               ccGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtg                                                                                 <  1:151298/149‑1 (MQ=255)
                                                                        cGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAAc                                                                        <  1:342960/149‑1 (MQ=255)
                                                                         gATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACg                                                                       >  1:162691/1‑149 (MQ=255)
                                                                          aTACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACgg                                                                      >  3:550081/1‑149 (MQ=255)
                                                                            aCTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCa                                                                    <  4:203567/149‑1 (MQ=255)
                                                                              tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAg                                                                                                 <  4:312354/118‑1 (MQ=255)
                                                                              tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAg                                                                                                 >  3:312354/1‑118 (MQ=255)
                                                                              tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCa                                                                    <  4:354688/147‑1 (MQ=255)
                                                                              tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGt                                                                  >  2:547167/1‑149 (MQ=255)
                                                                              tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGt                                                                  >  3:34181/1‑149 (MQ=255)
                                                                              tttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTAAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCa                                                                    >  3:354688/1‑147 (MQ=255)
                                                                                tCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTc                                                                <  2:162691/149‑1 (MQ=255)
                                                                                   aaTTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCca                                                             <  2:515063/149‑1 (MQ=255)
                                                                                     ttCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCcaca                                                           >  4:242682/1‑149 (MQ=255)
                                                                                     ttCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCcaca                                                           >  3:547293/1‑149 (MQ=255)
                                                                                          tCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTccc                                                      >  1:75327/1‑149 (MQ=255)
                                                                                          tCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTccc                                                      <  1:574326/149‑1 (MQ=255)
                                                                                             gCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGa                                                   >  2:409839/1‑149 (MQ=255)
                                                                                                 aTCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAtttt                                               >  1:117904/1‑149 (MQ=255)
                                                                                                  tCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAttttt                                              <  2:471668/149‑1 (MQ=255)
                                                                                                       gTGCGATTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCGAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCAccc                                         >  2:399642/1‑149 (MQ=255)
                                                                                                             ttCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCgg                                   >  3:184796/1‑149 (MQ=255)
                                                                                                                  tCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtggt                                                                               >  3:106625/1‑100 (MQ=255)
                                                                                                                  tCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtggt                                                                               <  4:106625/100‑1 (MQ=255)
                                                                                                                  tCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAg                              >  2:142951/1‑149 (MQ=255)
                                                                                                                  tCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAg                              >  2:158586/1‑149 (MQ=255)
                                                                                                                         gtcagtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCa                       <  3:66302/149‑1 (MQ=255)
                                                                                                                             gtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATc                        <  4:321875/144‑1 (MQ=255)
                                                                                                                             gtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATc                        >  3:321875/1‑144 (MQ=255)
                                                                                                                                cccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGTCCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAttt                                                <  4:460605/117‑1 (MQ=255)
                                                                                                                                cccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAttt                                                >  3:460605/1‑117 (MQ=255)
                                                                                                                                cccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGt                >  4:569967/1‑149 (MQ=255)
                                                                                                                                cccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGt                <  4:576726/149‑1 (MQ=255)
                                                                                                                                cccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGt                <  1:142951/149‑1 (MQ=255)
                                                                                                                                 ccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTg               >  2:9321/1‑149 (MQ=255)
                                                                                                                                 ccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTg               >  2:454529/1‑149 (MQ=255)
                                                                                                                                   gcgcCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGcc             <  4:547293/149‑1 (MQ=255)
                                                                                                                                     gccgccTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAg           <  3:284062/149‑1 (MQ=255)
                                                                                                                                     aaagccTGGCAGCCAGGCTTTAAATTTCAGACCATAACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACTCGACCGGAGAATCCCATCAGCTTGGTGCCAg           >  1:418187/4‑149 (MQ=255)
                                                                                                                                       cgccTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCa         <  1:8949/149‑1 (MQ=255)
                                                                                                                                              cTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCTTTAc  <  1:611527/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCTTTAC  >  NC_000913/2400541‑2400831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: