Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,401,329 A→G 5.5% M76T (ATG→ACG)  nuoF ← NADH:ubiquinone oxidoreductase, chain F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,401,3290AG 5.5% 243.8 / 3.2 72M76T (ATG→ACG) nuoFNADH:ubiquinone oxidoreductase, chain F
Reads supporting (aligned to +/- strand):  ref base A (35/33);  new base G (2/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

TCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTC  >  NC_000913/2401186‑2401477
                                                                                                                                               |                                                                                                                                                    
tCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCGCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAgg                                                                                                                                                 <  4:42515/149‑1 (MQ=255)
   gcgcAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTc                                                                                                                                              >  1:360332/1‑149 (MQ=255)
      cAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCAt                                                                                                                                           <  2:331302/149‑1 (MQ=255)
       aaaCGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCAtt                                                                                                                                          >  2:280736/1‑149 (MQ=255)
            cgGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAgg                                                                                                                                     <  4:73272/149‑1 (MQ=255)
                  tGAGCATACCTTCCACCAGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTc                                                                                                                               <  3:586530/149‑1 (MQ=255)
                    aGCATACCTTCCACCAGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCga                                                                                                                             <  1:146712/149‑1 (MQ=255)
                       aTACCTTCCACCAGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGaa                                                                                                                          >  2:426010/1‑149 (MQ=255)
                         aCCTTCCACCAGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGc                                                                                                                        <  4:614341/149‑1 (MQ=255)
                                accaGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCGTCAGGATCCATTTCAGGCCAGTCGAGAAGCCCGcgcc                                                                                                                 >  4:488405/1‑149 (MQ=255)
                                accaGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATc                                                                                                                                                    >  3:540523/1‑114 (MQ=255)
                                accaGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATc                                                                                                                                                    <  4:540523/114‑1 (MQ=255)
                                accaGCAGGTGCGGCAGTTGATCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCGTCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGcgcc                                                                                                                 >  1:496235/1‑149 (MQ=255)
                                    gcagGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGcgccgccg                                                                                                             >  1:505149/1‑149 (MQ=255)
                                             gCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTc                                                                                                    <  1:56559/149‑1 (MQ=255)
                                              cAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCa                                                                                                   <  1:281209/149‑1 (MQ=255)
                                                gTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGa                                                                                                 <  3:473053/149‑1 (MQ=255)
                                                gTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGa                                                                                                 >  4:473530/1‑149 (MQ=255)
                                                   gCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCa                                                                                              <  2:302720/149‑1 (MQ=255)
                                                     tCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGc                                                                                            >  1:166718/1‑149 (MQ=255)
                                                     tCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGc                                                                                            >  2:539922/1‑149 (MQ=255)
                                                       cATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGt                                                                                          >  4:309601/1‑149 (MQ=255)
                                                        aTCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTc                                                                                         >  3:265313/1‑149 (MQ=255)
                                                         tCAACAGGCGGTCTTTATAGGTGCCGGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCACACCAGCGTCt                                                                                        >  1:522868/1‑149 (MQ=255)
                                                         tCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCt                                                                                        <  4:322123/149‑1 (MQ=255)
                                                           aaCAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCttt                                                                                      >  2:350103/1‑149 (MQ=255)
                                                               ggCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTAcc                                                                                  >  3:273480/1‑149 (MQ=255)
                                                                   gTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGAt                                                                              >  1:113299/1‑149 (MQ=255)
                                                                   gTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGAt                                                                              >  3:36603/1‑149 (MQ=255)
                                                                    tCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGAtt                                                                             >  2:492426/1‑149 (MQ=255)
                                                                      tttATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTaa                                                                           <  1:280736/149‑1 (MQ=255)
                                                                          taGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCACACCAGCGTCTTTTACCTGATTAACGAt                                                                       <  4:290055/149‑1 (MQ=255)
                                                                           aGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGAtt                                                                      <  1:14727/149‑1 (MQ=255)
                                                                             gTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTc                                                                    <  1:426010/149‑1 (MQ=255)
                                                                             gTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCAAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTc                                                                    <  2:67513/149‑1 (MQ=255)
                                                                               gCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGt                                                                  >  2:191990/1‑149 (MQ=255)
                                                                                  cGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCg                                                               >  2:420905/1‑149 (MQ=255)
                                                                                    gCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGga                                                             <  2:37658/149‑1 (MQ=255)
                                                                                    gCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGga                                                             <  2:514448/149‑1 (MQ=255)
                                                                                      tCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGgaga                                                           <  1:350103/149‑1 (MQ=255)
                                                                                          tttCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGc                                                       >  3:56321/1‑149 (MQ=255)
                                                                                          tttCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGc                                                       >  1:356086/1‑149 (MQ=255)
                                                                                                cGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTc                                                 <  2:356086/149‑1 (MQ=255)
                                                                                                   cATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAgc                                              <  3:309601/149‑1 (MQ=255)
                                                                                                        cacaGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCtt                                         <  1:191990/149‑1 (MQ=255)
                                                                                                           agcagGTGACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTAcg                                      >  2:341000/1‑149 (MQ=255)
                                                                                                           agcagGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTAcg                                      >  2:61530/1‑149 (MQ=255)
                                                                                                           agcagGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTAcg                                      >  3:47007/1‑149 (MQ=255)
                                                                                                            gcagGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCgag                                                                                                                            <  2:357227/62‑1 (MQ=255)
                                                                                                            gcagGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCgag                                                                                                                            >  1:357227/1‑62 (MQ=255)
                                                                                                            gcagGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGaa                                                                                                                          >  4:169720/1‑64 (MQ=255)
                                                                                                            gcagGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGaa                                                                                                                          <  3:169720/64‑1 (MQ=255)
                                                                                                              agGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTAcgcgc                                   <  1:492426/149‑1 (MQ=255)
                                                                                                                      gATGTTCATGGATTGGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACCATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCATCGt                           <  2:522868/149‑1 (MQ=255)
                                                                                                                        tGTTCATGGATTCGTCTTTCGGCGTCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGtca                         <  2:496235/149‑3 (MQ=255)
                                                                                                                        tGTTCATGGATTCGTCTTTCGGCGTCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTaa                         <  3:488405/149‑1 (MQ=255)
                                                                                                                           tCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCg                      >  4:331701/1‑149 (MQ=255)
                                                                                                                           tCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCg                      >  3:413172/1‑149 (MQ=255)
                                                                                                                                gATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGttttt                 <  4:385589/149‑1 (MQ=255)
                                                                                                                                 aTTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTtg                >  4:157667/1‑149 (MQ=255)
                                                                                                                                 aTTCGTATTTCGGCATCAGTCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTtg                >  2:579024/1‑149 (MQ=255)
                                                                                                                                     gTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTtgctgc            <  2:121258/149‑1 (MQ=255)
                                                                                                                                      tCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCg           <  2:360332/149‑1 (MQ=255)
                                                                                                                                      tCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCg           <  4:569168/149‑1 (MQ=255)
                                                                                                                                      tCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCg           >  2:403810/1‑149 (MQ=255)
                                                                                                                                        tttCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGt         >  2:82896/1‑149 (MQ=255)
                                                                                                                                        tttCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGt         >  3:395984/1‑149 (MQ=255)
                                                                                                                                        tttCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGt         >  1:132027/1‑149 (MQ=255)
                                                                                                                                            ggCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTc     <  1:579024/149‑1 (MQ=255)
                                                                                                                                            ggCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTc     <  1:539922/149‑1 (MQ=255)
                                                                                                                                             gCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCg    >  2:288631/1‑149 (MQ=255)
                                                                                                                                             gCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTCTTTGCTGCGGTATTCg    >  2:399933/1‑149 (MQ=255)
                                                                                                                                               aTCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTc  >  1:135615/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TCAGCGCAAACGCGGAGATGAGCATACCTTCCACCAGCAGGTGCGGCAGTTGCTCCATCAACAGGCGGTCTTTATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCGTCTTTCGGCATCAGGCTCCATTTCAGGCCAGTCGAGAAGCCCGCGCCGCCGCGCCCTTTCAGACCAGCGTCTTTTACCTGATTAACGATTTCGTCCGGAGACAGCCCGGTCAGCGCCTTACGCGCGCCTTCGTAACCGTTTTTGCTGCGGTATTCGTC  >  NC_000913/2401186‑2401477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑