Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,571,172 T→C 8.0% N194D (AAT→GAT)  eutE ← aldehyde oxidoreductase, ethanolamine utilization protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,571,1720TC 8.0% 155.7 / 4.0 50N194D (AAT→GAT) eutEaldehyde oxidoreductase, ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base T (22/24);  new base C (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ACTACCGGCGGGTTGCCAGCGCCTGCGGCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGAT  >  NC_000913/2571024‑2571313
                                                                                                                                                    |                                                                                                                                             
agtACCGGCGGGTTGCCAGCGCCTGCGGCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGAtt                                                                                                                                               <  4:513775/147‑1 (MQ=255)
     cggcggGTTGCCAGCGCCTGCGGCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCca                                                                                                                                          <  2:39641/149‑1 (MQ=255)
     cggcggGTTGCCAGCGCCTGCGGCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCca                                                                                                                                          <  2:294694/149‑1 (MQ=255)
        cggGTTGCCAGCGCCTGCGGCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAg                                                                                                                                       <  3:531933/149‑1 (MQ=255)
               ccAGCGCCTGCGGCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAg                                                                                                                                >  3:107762/1‑149 (MQ=255)
               ccAGCGCCTGCGGCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAg                                                                                                                                <  2:492756/149‑1 (MQ=255)
                cAGCGCCTGCGGCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGt                                                                                                                               >  2:192621/1‑149 (MQ=255)
                  gcgcCTGCGGCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTaa                                                                                                                             >  3:554861/1‑149 (MQ=255)
                  gcgcCTGCGGCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTaa                                                                                                                             <  3:11643/149‑1 (MQ=255)
                          ggCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCg                                                                                                                     <  4:107762/149‑1 (MQ=255)
                           gCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCgg                                                                                                                    >  4:578243/1‑149 (MQ=255)
                               tCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCa                                                                                                                <  2:368683/149‑1 (MQ=255)
                                cAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCAc                                                                                                               <  1:191789/149‑1 (MQ=255)
                                     gTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATCTGCCACAGTAACCAGTAAGTTTTcc                                                                                                                      >  3:84544/1‑137 (MQ=255)
                                     gTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATCTGCCACAGTAACCAGTAAGTTTTcc                                                                                                                      <  4:84544/137‑1 (MQ=255)
                                          ttGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGcaac                                                                                                     >  3:386178/1‑149 (MQ=255)
                                          ttGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGcaac                                                                                                     <  1:311383/149‑1 (MQ=255)
                                          ttGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGcaac                                                                                                     <  4:554861/149‑1 (MQ=255)
                                                tgtTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGc                                                                                               <  3:475717/149‑1 (MQ=255)
                                                  tttACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCt                                                                                             >  4:305842/1‑149 (MQ=255)
                                                         gccgcTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGag                                                                                      >  2:48218/1‑149 (MQ=255)
                                                         gccgcTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGag                                                                                      >  1:549352/1‑149 (MQ=255)
                                                         gccgcTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGag                                                                                      <  3:273793/149‑1 (MQ=255)
                                                           cgcTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGagca                                                                                    <  4:63076/149‑1 (MQ=255)
                                                             cTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGagcagc                                                                                  <  1:153417/149‑1 (MQ=255)
                                                             cTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGagcagc                                                                                  >  1:189171/1‑149 (MQ=255)
                                                                   ctacCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATc                                                                            >  1:532561/1‑149 (MQ=255)
                                                                     acCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGa                                                                                       <  2:547248/136‑1 (MQ=255)
                                                                     acCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGa                                                                                       >  1:547248/1‑136 (MQ=255)
                                                                      ccGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCGACAATCGCCTGGTTGAGCAGCGTAATCGcc                                                                         >  4:98323/1‑149 (MQ=255)
                                                                         cTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGTAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGAttt                                                                                                                                              >  4:587258/1‑77 (MQ=255)
                                                                         cTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGAttt                                                                                                                                              <  3:587258/77‑1 (MQ=255)
                                                                             gccgccGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTggg                                                                  >  2:394813/1‑149 (MQ=255)
                                                                              ccgccgGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGga                                                                 <  4:202804/149‑1 (MQ=255)
                                                                                     ttACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTc                                                                                                                       <  4:73874/88‑1 (MQ=255)
                                                                                     ttACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTc                                                                                                                       >  3:73874/1‑88 (MQ=255)
                                                                                         caccaGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTcg                                                      >  3:465858/1‑149 (MQ=255)
                                                                                         caccaGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATCTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGga                                                                 <  4:193752/138‑1 (MQ=255)
                                                                                         caccaGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATCTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGga                                                                 >  3:193752/1‑138 (MQ=255)
                                                                                                 ggCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGa                                              <  2:563991/149‑1 (MQ=255)
                                                                                                       tACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCggg                                        >  2:39806/1‑149 (MQ=255)
                                                                                                                  ttGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGCAAAAATGAc                             <  3:216541/149‑1 (MQ=255)
                                                                                                                  ttGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGCAAAAATGAc                             <  1:89851/149‑1 (MQ=255)
                                                                                                                     aaCAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGCAAAAATGACGCt                          >  3:527091/1‑149 (MQ=255)
                                                                                                                        aaGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGCAAAAATGACGCTGtt                       >  3:80427/1‑149 (MQ=255)
                                                                                                                         aGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGCAAAAATGACGCTGTTg                      >  3:377272/1‑149 (MQ=255)
                                                                                                                          gCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGCAAAAATGACGCTGTTGc                     <  4:125187/149‑1 (MQ=255)
                                                                                                                           cGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCgg                                         >  3:600578/1‑128 (MQ=255)
                                                                                                                           cGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCgg                                         <  4:600578/128‑1 (MQ=255)
                                                                                                                                   ggTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGCAAAAATGACGCTGTTGCCCGCGGCaa            <  2:549352/149‑1 (MQ=255)
                                                                                                                                        cGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAgg       <  2:189171/149‑1 (MQ=255)
                                                                                                                                             tCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGAt  >  3:80922/1‑149 (MQ=255)
                                                                                                                                             tCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGAt  >  2:376716/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
ACTACCGGCGGGTTGCCAGCGCCTGCGGCAATCAGACGTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCAGGCCGATACCCGGAAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGAT  >  NC_000913/2571024‑2571313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑