Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,095,733 T→G 6.5% I212M (ATT→ATG)  yggS → UPF0001 family protein, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,095,7330TG 6.5% 262.4 / 8.7 77I212M (ATT→ATG) yggSUPF0001 family protein, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base T (32/40);  new base G (3/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

GATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATT  >  NC_000913/3095586‑3095881
                                                                                                                                                   |                                                                                                                                                    
gATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTg                                                                                                                                                     <  4:120434/149‑1 (MQ=255)
 aTGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGc                                                                                                                                                    >  1:143421/1‑149 (MQ=255)
 aTGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGc                                                                                                                                                    >  3:440856/1‑149 (MQ=255)
    gCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTGTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGc                                                                                                                                                 >  3:408191/1‑149 (MQ=255)
      aaTCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAg                                                                                                                                               <  4:73486/149‑1 (MQ=255)
        tCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGt                                                                                                                                             <  2:420425/149‑1 (MQ=255)
            tGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCa                                                                                                                                         <  4:408191/149‑1 (MQ=255)
             gcgcCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCAc                                                                                                                                        >  1:413698/1‑149 (MQ=255)
                    aGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGtt                                                                                                                                 <  4:491286/149‑1 (MQ=255)
                    aGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGtt                                                                                                                                 <  1:564146/149‑1 (MQ=255)
                     gTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTc                                                                                                                                <  2:143421/149‑1 (MQ=255)
                      tCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCg                                                                                                                               >  2:100540/1‑149 (MQ=255)
                      tCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCg                                                                                                                               >  4:558377/1‑149 (MQ=255)
                         gAATATGTCAGGCCGTTTGCAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACAATATGGAAGCCGCCATTGCTGCAGGTAGCACGATGGTTCGTAt                                                                                                                            <  4:422708/149‑1 (MQ=255)
                          aaTATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATc                                                                                                                           >  3:316368/1‑149 (MQ=255)
                          aaTATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATc                                                                                                                           >  4:59783/1‑149 (MQ=255)
                          aaTATGTAAGGCAGTTTGAAGTTGCACGCCAAATGACTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGAACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATc                                                                                                                           <  3:422970/149‑1 (MQ=255)
                           atatGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATc                                                                                                                           >  4:438185/1‑148 (MQ=255)
                           atatGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATc                                                                                                                           <  3:438185/148‑1 (MQ=255)
                                   ggCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCa                                                                                                                  >  4:325574/1‑149 (MQ=255)
                                    gCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCaa                                                                                                                 >  1:308484/1‑149 (MQ=255)
                                       gTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAAttt                                                                                                              <  2:467757/149‑1 (MQ=255)
                                        tttGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAAtttt                                                                                                             <  3:328032/149‑1 (MQ=255)
                                          tGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTg                                                                                                           <  4:71043/149‑1 (MQ=255)
                                                  cACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGt                                                                                                   <  3:192153/149‑1 (MQ=255)
                                                   aCGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTg                                                                                                  >  1:145110/1‑149 (MQ=255)
                                                     gCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGAt                                                                                                <  1:286889/149‑1 (MQ=255)
                                                       cAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTa                                                                                              >  3:483408/1‑149 (MQ=255)
                                                       cAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTa                                                                                              <  4:201949/149‑1 (MQ=255)
                                                                   gCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATa                                                                                  >  3:381214/1‑149 (MQ=255)
                                                                    cATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATaa                                                                                 <  1:495364/149‑1 (MQ=255)
                                                                      tttGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCACTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAgg                                                                               <  3:394498/149‑1 (MQ=255)
                                                                             gACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTaaa                                                                        <  2:290544/149‑1 (MQ=255)
                                                                              aCTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAg                                                                       <  4:316368/149‑1 (MQ=255)
                                                                                  aaaaCGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAAc                                                                   >  2:622407/1‑149 (MQ=255)
                                                                                       gcgcTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAGAAATAAGGAATTAAAGGAACGCCAt                                                              >  1:616758/1‑149 (MQ=255)
                                                                                       gcgcTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCAt                                                              <  4:202652/149‑1 (MQ=255)
                                                                                       gcgcTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCAt                                                              <  2:169117/149‑1 (MQ=255)
                                                                                          cTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGaa                                                           <  3:548262/149‑1 (MQ=255)
                                                                                          cTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGaa                                                           <  3:59783/149‑1 (MQ=255)
                                                                                              ccGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACg                                                       >  3:465169/1‑149 (MQ=255)
                                                                                              ccGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACg                                                       >  4:370089/1‑149 (MQ=255)
                                                                                               cGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGt                                                      <  1:364515/149‑1 (MQ=255)
                                                                                               cGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGt                                                      <  3:325574/149‑1 (MQ=255)
                                                                                                gCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGtt                                                     <  2:308484/149‑1 (MQ=255)
                                                                                                gCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGtt                                                     >  2:297814/1‑149 (MQ=255)
                                                                                                gCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGtt                                                     >  2:597691/1‑149 (MQ=255)
                                                                                                gCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGtt                                                     <  1:500426/149‑1 (MQ=255)
                                                                                                     tCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACtt                                                >  3:531582/1‑149 (MQ=255)
                                                                                                     tCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACtt                                                >  3:481395/1‑149 (MQ=255)
                                                                                                       gACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTc                                              <  1:597691/149‑1 (MQ=255)
                                                                                                        acacGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTcc                                             >  1:430212/1‑149 (MQ=255)
                                                                                                        acacGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTcc                                             <  4:440856/149‑1 (MQ=255)
                                                                                                         cacGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCt                                            <  2:246663/149‑1 (MQ=255)
                                                                                                            gCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCt                                         >  2:5324/1‑149 (MQ=255)
                                                                                                              tctctctGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGc                                          <  3:304037/146‑1 (MQ=255)
                                                                                                              tctctctGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGc                                          >  4:304037/1‑146 (MQ=255)
                                                                                                                 ctctGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCaa                                    <  4:556591/149‑1 (MQ=255)
                                                                                                                  tctGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAAc                                   >  1:423597/1‑149 (MQ=255)
                                                                                                                  tctGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAAc                                   >  2:425646/1‑149 (MQ=255)
                                                                                                                  tctGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAAc                                   <  4:483408/149‑1 (MQ=255)
                                                                                                                      ggAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTc                               <  2:413698/149‑1 (MQ=255)
                                                                                                                           gTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGa                          <  1:5324/149‑1 (MQ=255)
                                                                                                                                aCGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGt                     >  1:1236/1‑149 (MQ=255)
                                                                                                                                aCGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGt                     <  3:409310/149‑1 (MQ=255)
                                                                                                                                     atGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGACCGCCATGAATACGtt                                                     <  1:150789/112‑1 (MQ=255)
                                                                                                                                     atGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGtt                                                     >  2:150789/1‑112 (MQ=255)
                                                                                                                                      tGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCa               <  2:145110/149‑1 (MQ=255)
                                                                                                                                       ggAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCT‑AAAAATAAGGAATTAAAGGAACGCCATGTATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCATg             >  3:298563/1‑149 (MQ=255)
                                                                                                                                        gAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCAt                             >  2:95613/1‑133 (MQ=255)
                                                                                                                                        gAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCAt                             <  1:95613/133‑1 (MQ=255)
                                                                                                                                         ctgccgcaATGGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCATgg            >  2:159739/3‑149 (MQ=255)
                                                                                                                                           gccgcaATGGCGGCAGGTAGCACGATGGTTCGTATCg                                                                                                                          >  1:21453/1‑37 (MQ=255)
                                                                                                                                           gccgcaATGGCGGCAGGTAGCACGATGGTTCGTATCg                                                                                                                          <  2:21453/37‑1 (MQ=255)
                                                                                                                                           gccgcaATGGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTaaa                                                                        <  2:542927/87‑1 (MQ=255)
                                                                                                                                           gccgcaATGGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTaaa                                                                        >  1:542927/1‑87 (MQ=255)
                                                                                                                                                 aTTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCATGGTGCTGtta    >  3:388781/1‑149 (MQ=255)
                                                                                                                                                  ttGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCATGGTGCTGttat   >  4:314168/1‑149 (MQ=255)
                                                                                                                                                  ttGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCATGGTGCTGttat   <  3:181740/149‑1 (MQ=255)
                                                                                                                                                   tGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCATGGTGCTGttatt  <  1:622407/149‑1 (MQ=255)
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GATGGCAATCCCTGCGCCTGAGTCAGAATATGTAAGGCAGTTTGAAGTTGCACGCCAAATGGCTGTAGCATTTGCCGGACTGAAAACGCGCTACCCGCATATCGACACGCTCTCTCTGGGAATGTCGGACGATATGGAAGCCGCCATTGCGGCAGGTAGCACGATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATT  >  NC_000913/3095586‑3095881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑