| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,236,076 | C→T | 7.1% | H39H (CAC→CAT) | ygjP → | putative metal dependent hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,236,076 | 0 | C | T | 7.1% | 175.5 / 3.7 | 56 | H39H (CAC→CAT) | ygjP | putative metal dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (27/25); new base T (2/2); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01 | |||||||||||
TATACCCTTTTTCTCTTTTTCGTGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAATAAGATCCATGTGCTAAAAAACGCGCTCGGGTTACATACCGCTGTTTCTC > NC_000913/3235929‑3236224 | tataCCCTTTTTCTCTTTTTCGTGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACg > 3:537433/1‑149 (MQ=255) ccTTTTTCTCTTTTTCGTGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTAc > 2:150637/1‑149 (MQ=255) cTTTTTCTCTTTTTCGTGGGCGGTTATGAGCAATCTTACTTATCTTTAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACATGACTACg > 1:287555/1‑149 (MQ=255) cTTTTTCTCTTTTTCGTGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACATGACTACg > 3:125666/1‑149 (MQ=255) cTTTTTCTCTTTTTCGTGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACg > 4:510267/1‑149 (MQ=255) tttttCTCTTTTTCGTGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTACCCGGGAACACACGACTACGc > 1:364311/1‑149 (MQ=255) tctTTTTCGTGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGa > 3:417064/1‑149 (MQ=255) ttttCGTGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAAAGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATACGGGAACACACGACTACGCCACCGATaa > 2:345409/1‑149 (MQ=255) tttCGTGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATaaa < 3:585164/149‑1 (MQ=255) tttCGTGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATaaa < 3:51409/149‑1 (MQ=255) ttCGTGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAg < 3:13339/149‑1 (MQ=255) tGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCct > 2:2481/1‑149 (MQ=255) tacttaTCTTCAGGGTTATCACGAGCAGCTACTTCCCCAGGTGCGAACACTGATTAACCAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGTACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGCTc < 2:435962/149‑1 (MQ=255) ttaTCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGa < 3:458780/149‑1 (MQ=255) ttaTCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGa < 3:481201/149‑1 (MQ=255) taTCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGaa > 2:371091/1‑149 (MQ=255) aTCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGaaa > 4:159086/1‑149 (MQ=255) aTCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGaaa < 3:510267/149‑1 (MQ=255) aGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCctct < 1:549755/120‑1 (MQ=255) aGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCctct > 2:549755/1‑120 (MQ=255) gTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGttt > 2:601700/1‑149 (MQ=255) ttATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGtt > 1:405715/1‑147 (MQ=255) ttATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGtt < 2:405715/147‑1 (MQ=255) cccGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAacacac > 1:117933/1‑87 (MQ=255) cccGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAacacac < 2:117933/87‑1 (MQ=255) gcagcTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATg < 1:150637/149‑1 (MQ=255) gcagcTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATg > 1:483075/1‑149 (MQ=255) cagcTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGc > 1:466199/1‑149 (MQ=255) agcTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGcc < 1:334887/149‑1 (MQ=255) cTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCccgcc > 1:431616/1‑149 (MQ=255) ttCCCGGGGGCGAACACTGATAAACAAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACCCACGACTACGTCACCAATAAAGCCCTCTGCCAATATTCCCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGa < 4:280085/149‑1 (MQ=255) ttCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACACAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGa < 3:224389/149‑1 (MQ=255) tCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGAt > 3:171403/1‑149 (MQ=255) ccAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCa < 1:193076/149‑1 (MQ=255) tGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACATGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATaaa < 2:287555/149‑1 (MQ=255) tGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACATGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATaaa < 4:125666/149‑1 (MQ=255) gAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTg > 3:433773/1‑149 (MQ=255) aaCACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGa < 2:412982/149‑1 (MQ=255) aaCACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGa < 4:537433/149‑1 (MQ=255) acacTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGAt < 1:371091/149‑1 (MQ=255) gATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATg > 1:318427/1‑149 (MQ=255) aCGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAAt > 4:13197/1‑149 (MQ=255) aCGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAAt > 2:563564/1‑149 (MQ=255) cGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAATa < 3:146460/149‑1 (MQ=255) aaCAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACa < 3:231359/144‑1 (MQ=255) aaCAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACa > 4:231359/1‑144 (MQ=255) cAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAATAAGAt < 4:542258/149‑1 (MQ=255) aGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAATAAGATc > 1:570273/1‑149 (MQ=255) gCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCa > 3:340911/1‑96 (MQ=255) gCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCa < 4:340911/96‑1 (MQ=255) tCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAATAAGATCCAtg > 1:123459/1‑149 (MQ=255) cTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCTCCGCCGATCAATAAAGTGATGTATGACAATAAGATCCAtgt < 3:404074/149‑1 (MQ=255) gtgCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAATAAGATCCATGTGCTaaaaaa > 3:272862/1‑149 (MQ=255) cTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAATAAGATCCATGTGCTAAAAAAcgc < 2:364311/149‑1 (MQ=255) aaCGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAATAAGATCCATGTGCTAAAAAACGCGCTCggg < 4:173741/149‑1 (MQ=255) aaCGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAATAAGATCCATGTGCTAAAAAACGCGCTCggg > 1:70181/1‑149 (MQ=255) tCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCa > 4:168788/1‑66 (MQ=255) tCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCa < 3:168788/66‑1 (MQ=255) acacGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAATAAGATCCATGTGCTAAAAAACGCGCTCGGGTTACATACCGCTGttt > 3:348693/1‑149 (MQ=255) cacGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATc < 1:577159/81‑1 (MQ=255) cacGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCACGCCGATc > 2:577159/1‑81 (MQ=255) acGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAATAAGATCCATGTGCTAAAAAACGCGCTCGGGTTACATACCGCTGTTtct > 2:608116/1‑149 (MQ=255) cGACTACGCAACCGATAAAGCCCGCTGGAAATATACTCAGGATCTGAAACATCAGTTTCTGCGTAATGCCCCGCCCATCAATAAAGTGATGTATGACAATAAGATCCATGTGCTAAAAAACGCGATCGGGTTACATACCGCTGTTtctc < 3:524006/149‑1 (MQ=255) | TATACCCTTTTTCTCTTTTTCGTGGGCGGTTATGAGCAATCTTACTTATCTTCAGGGTTATCCCGAGCAGCTACTTTCCCAGGTGCGAACACTGATTAACGAACAGCGTCTGGGCGATGTGCTGGCAAAACGCTATCCGGGAACACACGACTACGCCACCGATAAAGCCCTCTGGCAATATACTCAGGATCTGAAAAATCAGTTTCTGCGTAATGCCCCGCCGATCAATAAAGTGATGTATGACAATAAGATCCATGTGCTAAAAAACGCGCTCGGGTTACATACCGCTGTTTCTC > NC_000913/3235929‑3236224 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |