Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,080,024 G→C 6.0% intergenic (+55/+275) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,080,0240GC 6.0% 293.2 / 6.7 83intergenic (+55/+275)yiiF/fdhEputative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base G (34/44);  new base C (2/3);  total (36/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGG  >  NC_000913/4079879‑4080171
                                                                                                                                                 |                                                                                                                                                   
gCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATg                                                                                                                                                  >  2:385839/1‑149 (MQ=255)
 cAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATgc                                                                                                                                                 >  4:222313/1‑149 (MQ=255)
  aaaCAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATgcg                                                                                                                                                >  4:574099/1‑149 (MQ=255)
  aaaCAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATgcg                                                                                                                                                >  3:97675/1‑149 (MQ=255)
    aCAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATgcggc                                                                                                                                              <  4:480775/149‑1 (MQ=255)
     cAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATgcggcg                                                                                                                                             <  1:276015/149‑1 (MQ=255)
       gCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTg                                                                                                                                           <  1:421301/149‑1 (MQ=255)
        cAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGa                                                                                                                                          >  2:435308/1‑149 (MQ=255)
        cAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCACGCCTCATCCGGCATGa                                                                                                                                          <  2:150152/149‑1 (MQ=255)
            aaCCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGc                                                                                                                                      <  1:108680/149‑1 (MQ=255)
            aaCCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAAGCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGc                                                                                                                                      <  2:466435/149‑1 (MQ=255)
             aCCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                     <  1:111771/149‑1 (MQ=255)
              ccAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCt                                                                                                                                    <  2:610229/149‑1 (MQ=255)
              ccAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCt                                                                                                                                    <  1:385839/149‑1 (MQ=255)
               cAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCtt                                                                                                                                   <  1:94863/149‑1 (MQ=255)
                aGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                  >  1:389413/1‑149 (MQ=255)
                  tgtTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                <  3:74563/149‑1 (MQ=255)
                   gtTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                               >  1:322166/1‑149 (MQ=255)
                     tCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                             <  3:273151/149‑1 (MQ=255)
                       cTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                           >  1:262016/1‑149 (MQ=255)
                           cATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACa                                                                                                                       >  2:365954/1‑149 (MQ=255)
                            aTCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaa                                                                                                                      >  2:343451/1‑149 (MQ=255)
                             tCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaa                                                                                                                     <  2:439838/149‑1 (MQ=255)
                             tCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaa                                                                                                                     <  1:343451/149‑1 (MQ=255)
                               tGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGt                                                                                                                   >  3:605907/1‑149 (MQ=255)
                                ggCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTc                                                                                                                  <  4:161901/149‑1 (MQ=255)
                                ggCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTc                                                                                                                  <  2:171196/149‑1 (MQ=255)
                                 gCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCg                                                                                                                 >  3:73856/1‑149 (MQ=255)
                                  cAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGt                                                                                                                <  1:569910/149‑1 (MQ=255)
                                            aGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCaa                                                                                                      <  2:262016/149‑1 (MQ=255)
                                            aGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCaa                                                                                                      >  2:566704/1‑149 (MQ=255)
                                            aGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCaa                                                                                                      <  2:353765/149‑1 (MQ=255)
                                             ggAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAAt                                                                                                     <  3:574099/149‑1 (MQ=255)
                                                aGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTa                                                                                                  <  2:475726/149‑1 (MQ=255)
                                                 gATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTAt                                                                                                 >  3:481681/1‑149 (MQ=255)
                                                    ggTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGc                                                                                              <  2:505252/149‑1 (MQ=255)
                                                     gtgtCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCa                                                                                             <  1:566704/149‑1 (MQ=255)
                                                        tCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAGAAAGTCGTATAAATTCAATGTATTGCAGtt                                                                                          >  1:405002/1‑149 (MQ=255)
                                                        tCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGtt                                                                                          >  1:430762/1‑149 (MQ=255)
                                                          gAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTa                                                                                        <  1:494764/149‑1 (MQ=255)
                                                           aaTATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTAt                                                                                       <  1:452308/149‑1 (MQ=255)
                                                           aaTATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTAt                                                                                       >  1:435261/1‑149 (MQ=255)
                                                            atatCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTAtt                                                                                      <  4:73856/149‑1 (MQ=255)
                                                                    gTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCaa                                                                                                      >  4:312649/1‑125 (MQ=255)
                                                                    gTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCaa                                                                                                      <  3:312649/125‑1 (MQ=255)
                                                                     tAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCt                                                                             <  3:313573/149‑1 (MQ=255)
                                                                      aaGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTg                                                                            >  4:139228/1‑149 (MQ=255)
                                                                         cTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGCATccggcat                                                                                                                                            <  4:599343/82‑3 (MQ=255)
                                                                         cTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGCATccggcat                                                                                                                                            <  1:202211/82‑3 (MQ=37)
                                                                         cTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGCATccggcat                                                                                                                                            >  2:202211/1‑80 (MQ=255)
                                                                         cTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGCATccggcat                                                                                                                                            >  3:599343/1‑80 (MQ=255)
                                                                          tGCGCGAGGAATGGTAATGGGACAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTACAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATaa                                                                        <  4:511925/149‑1 (MQ=255)
                                                                            cgcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagc                                                                      <  2:322166/149‑1 (MQ=255)
                                                                             gcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATaaa                                                                                                           >  2:458195/1‑111 (MQ=255)
                                                                             gcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATaaa                                                                                                           <  1:458195/111‑1 (MQ=255)
                                                                               gAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgca                                                                   <  1:207756/149‑1 (MQ=255)
                                                                                aGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATCAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgcag                                                                  >  3:54808/1‑149 (MQ=255)
                                                                                     tGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCAt                                                             <  1:219954/149‑1 (MQ=255)
                                                                                       gtcaTGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAAt                                                                                                     <  3:394670/104‑1 (MQ=255)
                                                                                       gTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCa                                                           >  1:417765/1‑149 (MQ=255)
                                                                                       gTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTATTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCa                                                           <  4:54808/149‑1 (MQ=255)
                                                                                       gTAATGGGAAAAAATGATGTCAATCACATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAAt                                                                                                     >  4:394670/1‑107 (MQ=255)
                                                                                             ggAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                            <  4:432222/78‑1 (MQ=255)
                                                                                             ggAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAAt                                                     >  3:531388/1‑149 (MQ=255)
                                                                                             ggAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCATTATCCGGc                                                                                                                            >  3:432222/1‑78 (MQ=255)
                                                                                              gAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATc                                                    <  2:548394/149‑1 (MQ=255)
                                                                                               aaaaaaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCt                                                   <  2:405002/149‑1 (MQ=255)
                                                                                                   aaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACg                                               >  4:336748/1‑149 (MQ=255)
                                                                                                      gatgTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCtt                                            <  2:430762/149‑1 (MQ=255)
                                                                                                         gTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGtc                                         <  1:612012/149‑1 (MQ=255)
                                                                                                          tCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAAAGCAGCGCATCAGGCAATCTTACGCTTGtca                                        >  2:352013/1‑149 (MQ=255)
                                                                                                            aaTCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGtcatc                                      >  1:207686/1‑149 (MQ=255)
                                                                                                            aaTCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGtcatc                                      >  1:190012/1‑149 (MQ=255)
                                                                                                            aaTCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGtcatc                                      >  3:407121/1‑149 (MQ=255)
                                                                                                                 gATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGtct                                 >  3:608276/1‑149 (MQ=255)
                                                                                                                         aCAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCa                         >  4:118533/1‑149 (MQ=255)
                                                                                                                           aaCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGtc                                  <  1:541866/138‑1 (MQ=255)
                                                                                                                           aaCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGtc                                  >  2:541866/1‑138 (MQ=255)
                                                                                                                            aCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATaa                      <  4:481681/149‑1 (MQ=255)
                                                                                                                                 cgcgTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTa                 <  1:286936/149‑1 (MQ=255)
                                                                                                                                 cgcgTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTa                 <  2:207686/149‑1 (MQ=255)
                                                                                                                                             gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggtg      >  3:56637/1‑148 (MQ=255)
                                                                                                                                             gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggtg      <  4:56637/148‑1 (MQ=255)
                                                                                                                                                ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACGGTATTGATGtggtggtgg  <  4:531388/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGG  >  NC_000913/4079879‑4080171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: