Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,080,616 | C→A | 6.6% | G205C (GGC→TGC) | fdhE ← | formate dehydrogenase formation protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,080,616 | 0 | C | A | 6.6% | 320.1 / 11.2 | 91 | G205C (GGC→TGC) | fdhE | formate dehydrogenase formation protein |
Reads supporting (aligned to +/- strand): ref base C (40/45); new base A (3/3); total (43/48) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-01 |
TTTCGGCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCTTTA > NC_000913/4080469‑4080764 | tttCGGCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCa > 3:199999/1‑149 (MQ=255) ttCGGCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCaa > 2:245796/1‑149 (MQ=255) cGGCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTCACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAAtt < 4:258100/149‑1 (MQ=255) gCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTg > 2:614276/1‑149 (MQ=255) cTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGc < 4:112957/149‑1 (MQ=255) tttAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCa > 1:507807/1‑149 (MQ=255) tttAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCa > 3:530928/1‑149 (MQ=255) cgcgGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGc < 4:62158/149‑1 (MQ=255) cgGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCactcat < 4:310490/149‑6 (MQ=255) cgGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCactcag < 4:74796/149‑6 (MQ=255) ccTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGac > 1:449474/1‑149 (MQ=255) cTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGaca > 2:194655/1‑149 (MQ=255) gTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACAcc > 4:472850/1‑149 (MQ=255) ttCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCg < 2:74922/149‑1 (MQ=255) tCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCAt < 2:387402/149‑1 (MQ=255) cATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATa < 4:228021/149‑1 (MQ=255) ccAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGaa < 1:504359/149‑1 (MQ=255) cAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAAc > 1:463801/1‑149 (MQ=255) cAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAAc > 3:550232/1‑149 (MQ=255) cAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAAc < 4:15746/149‑1 (MQ=255) gCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCTTTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACca < 3:516432/149‑1 (MQ=255) gCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACca > 1:341664/1‑149 (MQ=255) gTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCac < 1:581469/120‑2 (MQ=255) gTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCac > 2:581469/1‑119 (MQ=255) tAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCgg < 2:350113/149‑1 (MQ=255) tAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCgg < 2:463801/149‑1 (MQ=255) gCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCaa > 2:61557/1‑149 (MQ=255) tttGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATAtt < 2:294218/149‑1 (MQ=255) tGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCGCAAACCGGGCAATATTGa < 2:507807/149‑1 (MQ=255) gCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGAc < 1:183978/149‑1 (MQ=255) cGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGt > 3:438184/1‑149 (MQ=255) cGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGt > 2:539242/1‑149 (MQ=255) ctctGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCAGCATGCTGGAAACCGGCATAGAACCACAAACCGGGCAATATTGACGTTg > 4:47376/1‑149 (MQ=255) ctctGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTg < 4:530928/149‑1 (MQ=255) tGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCTGCATAGAACCACAACCCGAGCAATATTGACGTTGCTc < 4:79922/149‑1 (MQ=255) tCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGcc > 2:487837/1‑149 (MQ=255) tgctgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGGTGCTCGCCGTATTCa > 1:543542/1‑149 (MQ=255) gctgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAg < 1:22325/149‑1 (MQ=255) tgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAgcg > 2:362212/1‑149 (MQ=255) aTTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCgg < 1:128493/149‑1 (MQ=255) ttttACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCggg > 2:41656/1‑149 (MQ=255) cgcgCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTg < 2:327969/149‑1 (MQ=255) cgcACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGcc < 3:438797/149‑1 (MQ=255) cACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCgg < 2:560282/149‑1 (MQ=255) aCTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCggg > 4:340691/1‑149 (MQ=255) aCTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCggg < 3:472850/149‑1 (MQ=255) aCTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCggg > 1:203511/1‑149 (MQ=255) aCTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCggg < 3:499676/149‑1 (MQ=255) tACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGa < 3:619937/149‑1 (MQ=255) aCGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGAt < 2:457422/149‑1 (MQ=255) gTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCa < 2:266082/149‑1 (MQ=255) gTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCa > 3:485679/1‑149 (MQ=255) gCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGa < 2:60394/149‑1 (MQ=255) gCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGa < 4:438184/149‑1 (MQ=255) cATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGAtt < 2:27082/149‑1 (MQ=255) cATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGAtt < 4:263139/149‑1 (MQ=255) cATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGAtt > 1:580406/1‑149 (MQ=255) aTTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTg < 1:354675/149‑1 (MQ=255) ttCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGtt > 4:618726/1‑107 (MQ=255) ttCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGtt < 3:618726/107‑1 (MQ=255) ttCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTgg > 2:105656/1‑149 (MQ=255) tttCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATc > 3:112936/1‑149 (MQ=255) acacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgcg > 3:6172/1‑149 (MQ=255) acaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGcc > 4:163216/1‑149 (MQ=255) acaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGcc > 1:468990/1‑149 (MQ=255) aGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCa < 1:211135/149‑1 (MQ=255) gTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCg < 3:490956/59‑1 (MQ=255) gTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCg > 4:490956/1‑59 (MQ=255) gTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGt > 2:9287/1‑149 (MQ=255) gcagtgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTaga < 1:61557/149‑1 (MQ=255) gcagtgcagGTAACGTAGCCCCTGAGTGGTACCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTaga < 2:62847/149‑1 (MQ=255) agtgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTGTTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGc > 4:18585/1‑149 (MQ=255) tgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGa < 1:365322/149‑1 (MQ=255) tgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGa < 4:199999/149‑1 (MQ=255) gcagGTAACGTAGCCCCTGTGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGAc > 3:493905/1‑149 (MQ=255) gcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGAc < 3:254186/149‑1 (MQ=255) agGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAg > 3:65040/1‑149 (MQ=255) gTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTg > 4:242891/1‑149 (MQ=255) gTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGcc < 4:440643/149‑1 (MQ=255) ccccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGa > 1:87714/1‑149 (MQ=255) ccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATa > 1:554085/1‑149 (MQ=255) ccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATa > 2:146265/1‑149 (MQ=255) cagcatggtgCAAATTGGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATaa > 1:68650/6‑149 (MQ=255) cTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATaa > 1:139783/1‑149 (MQ=255) gAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAAc < 2:177055/149‑1 (MQ=255) gAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAAc < 4:112936/149‑1 (MQ=255) catggtgCAAATTGGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACg > 3:520606/3‑149 (MQ=255) gtggtgCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACgg > 1:400517/1‑149 (MQ=255) tgCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCt < 1:487837/149‑1 (MQ=255) tgCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCt < 1:194655/149‑1 (MQ=255) gCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCtt < 1:41656/149‑1 (MQ=255) cAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCTTTa > 1:609191/1‑149 (MQ=255) | TTTCGGCTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCTTTA > NC_000913/4080469‑4080764 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |