Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,395,454 T→C 7.5% C463C (TGT→TGC)  nnr → bifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,395,4540TC 7.5% 204.8 / 4.1 64C463C (TGT→TGC) nnrbifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
Reads supporting (aligned to +/- strand):  ref base T (28/31);  new base C (3/2);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGA  >  NC_000913/4395306‑4395596
                                                                                                                                                    |                                                                                                                                              
ccGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCtgt                                                                                                                                                <  3:508887/149‑1 (MQ=255)
      ccGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGc                                                                                                                                          <  2:146813/149‑1 (MQ=255)
             cgccgcCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcg                                                                                                                                   >  4:251750/1‑149 (MQ=255)
                    cATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCTTGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACgg                                                                                                                            <  1:419302/149‑1 (MQ=255)
                      tCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTg                                                                                                                          <  4:438231/149‑1 (MQ=255)
                       ccTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGc                                                                                                                         >  3:385239/1‑149 (MQ=255)
                            cGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAg                                                                                                                    <  4:485952/149‑1 (MQ=255)
                             gCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGc                                                                                                                   <  3:493110/149‑1 (MQ=255)
                                 tAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGAc                                                                                                               <  4:582781/149‑1 (MQ=255)
                                    gCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGATGTa                                                                                                            >  4:616454/1‑149 (MQ=255)
                                      attattGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcg                                                                                                                                   <  2:538979/124‑1 (MQ=255)
                                      attattGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcg                                                                                                                                   >  1:538979/1‑124 (MQ=255)
                                              tGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGgcgc                                                                                                  >  4:559207/1‑149 (MQ=255)
                                              tGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGgcgc                                                                                                  >  4:592954/1‑149 (MQ=255)
                                                ccGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGt                                                                                                >  1:29750/1‑149 (MQ=255)
                                                 cGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGtt                                                                                               >  4:617920/1‑149 (MQ=255)
                                                 cGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGtt                                                                                               >  2:134683/1‑149 (MQ=255)
                                                 cGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGtt                                                                                               <  2:29750/149‑1 (MQ=255)
                                                              aTGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCgg                                                                                  <  3:211722/149‑1 (MQ=255)
                                                                    aGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCt                                                                            <  1:505946/149‑1 (MQ=255)
                                                                       ggcggcATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGc                                                                         <  3:251750/149‑1 (MQ=255)
                                                                             aTGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGa                                                                   >  4:479195/1‑149 (MQ=255)
                                                                                  cGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATctct                                                              <  1:427561/149‑1 (MQ=255)
                                                                                    aTGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCttt                                                            <  4:387180/149‑1 (MQ=255)
                                                                                      gtgCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTc                                                          <  4:4016/149‑1 (MQ=255)
                                                                                         ctctctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCAc                                                       >  2:151145/1‑149 (MQ=255)
                                                                                          tctctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACg                                                      >  3:579046/1‑149 (MQ=255)
                                                                                            tctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGAt                                                                               <  1:214360/122‑1 (MQ=255)
                                                                                            tctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTCTCCACGCt                                                    >  4:85712/1‑149 (MQ=255)
                                                                                            tccggTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGAt                                                                               >  2:214360/4‑122 (MQ=255)
                                                                                                gTATTATTGGTGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCAc                                                       <  2:299822/142‑1 (MQ=255)
                                                                                                gTATTATTGGTGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCAc                                                       >  1:299822/1‑142 (MQ=255)
                                                                                                      ttGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTa                                            >  2:257702/1‑147 (MQ=255)
                                                                                                      ttGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTa                                            <  1:257702/147‑1 (MQ=255)
                                                                                                      ttGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTAtt                                          <  3:334302/149‑1 (MQ=255)
                                                                                                      ttGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTAtt                                          >  3:476629/1‑149 (MQ=255)
                                                                                                       tGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTg                                         <  3:261064/149‑1 (MQ=255)
                                                                                                           gcATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTaa                                     <  2:299800/149‑1 (MQ=255)
                                                                                                           gcATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTaa                                     <  2:323406/149‑1 (MQ=255)
                                                                                                           gcATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTaa                                     >  3:451189/1‑149 (MQ=255)
                                                                                                           gcATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTCTCCACGCTACAGCGTATTGTTaa                                     <  3:85712/149‑1 (MQ=255)
                                                                                                            cATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAAc                                    <  1:151145/149‑1 (MQ=255)
                                                                                                             aTTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAAcc                                   <  3:617920/149‑1 (MQ=255)
                                                                                                              ttGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAAcgcg                                                                                     >  3:4035/1‑98 (MQ=255)
                                                                                                              ttGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAAcgcg                                                                                     >  3:340721/1‑98 (MQ=255)
                                                                                                              ttGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAAcgcg                                                                                     <  4:340721/98‑1 (MQ=255)
                                                                                                              ttGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAAcgcg                                                                                     <  4:4035/98‑1 (MQ=255)
                                                                                                                   tGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAg                             >  3:485183/1‑149 (MQ=255)
                                                                                                                      gCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCCCGCTACAGCGTATTGTTAACCCGGAAGTGa                          <  4:419718/149‑1 (MQ=255)
                                                                                                                        aaaaaCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACt                        >  1:577862/1‑149 (MQ=255)
                                                                                                                        aaaaaCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACt                        >  4:521618/1‑149 (MQ=255)
                                                                                                                         aaaaCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTg                                                                           <  3:108686/97‑1 (MQ=255)
                                                                                                                         aaaaCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTg                                                                           >  4:108686/1‑97 (MQ=255)
                                                                                                                               gTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATaaaa                 >  3:138787/1‑149 (MQ=255)
                                                                                                                                tCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATaaaaa                <  2:614334/149‑1 (MQ=255)
                                                                                                                                   ccGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCCCGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCa             <  2:45943/149‑1 (MQ=255)
                                                                                                                                   ccGTATGATGCAGCCGGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCa             >  4:458647/1‑149 (MQ=255)
                                                                                                                                      tATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCatga          <  4:451189/149‑1 (MQ=255)
                                                                                                                                       atgatgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCatgat         >  2:590138/1‑149 (MQ=255)
                                                                                                                                         gacacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATaaaaa                <  2:439007/136‑1 (MQ=255)
                                                                                                                                         gacacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATaaaaa                >  1:439007/5‑140 (MQ=255)
                                                                                                                                         gacacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCa             >  3:212560/5‑143 (MQ=255)
                                                                                                                                         gacacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCa             <  4:212560/139‑1 (MQ=255)
                                                                                                                                         gacacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCAtgatga       >  3:550466/5‑149 (MQ=255)
                                                                                                                                              aGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGa  >  1:206194/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGA  >  NC_000913/4395306‑4395596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑