Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,517,511 G→C 8.3% S69R (AGC→AGG fecR ← anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,517,5110GC 8.3% 244.7 / 11.1 72S69R (AGC→AGGfecRanti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
Reads supporting (aligned to +/- strand):  ref base G (23/43);  new base C (3/3);  total (26/46)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

TGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCA  >  NC_000913/4517363‑4517658
                                                                                                                                                    |                                                                                                                                                   
tGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCgg                                                                                                                                                     >  1:175404/1‑149 (MQ=255)
tGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCgg                                                                                                                                                     >  4:243532/1‑149 (MQ=255)
   cTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTg                                                                                                                                                  <  4:469590/149‑1 (MQ=255)
      tgCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCTAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGCCTGGcg                                                                                                                                               >  3:486906/1‑148 (MQ=255)
      tgCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCTAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGCCTGGcg                                                                                                                                               >  1:84328/1‑148 (MQ=255)
      tgCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGCCTGGcg                                                                                                                                               >  1:4497/1‑148 (MQ=255)
       gCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCaa                                                                                                                                              <  3:328242/149‑1 (MQ=255)
           ttGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCACTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTc                                                                                                                                          <  4:423678/149‑1 (MQ=255)
               cggtgcggtAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCa                                                                                                                                      <  1:23830/149‑1 (MQ=255)
                 gtgcggtAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAgg                                                                                                                                    >  1:311956/1‑149 (MQ=255)
                    cggtAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCTAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGCCTGGCGACGTTGCCAgccgg                                                                                                                                 <  4:486906/149‑5 (MQ=255)
                    cggtAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCTAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGCCTGGCGACGTTGCCAgccgg                                                                                                                                 <  2:84328/149‑5 (MQ=255)
                    cggtAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGcac                                                                                                                                 >  2:248522/1‑149 (MQ=255)
                    cggtAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGCCTGGCGACGTTGCCAgccgg                                                                                                                                 <  2:4497/149‑5 (MQ=255)
                          tCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGcc                                                                                                                           >  4:298951/1‑149 (MQ=255)
                            tGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCaa                                                                                                                         <  3:10380/149‑1 (MQ=255)
                            tGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCaa                                                                                                                         >  4:110775/1‑149 (MQ=255)
                              cccGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGc                                                                                                                       <  2:423907/149‑1 (MQ=255)
                                  cAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGt                                                                                                                   <  4:79218/149‑1 (MQ=255)
                                        ttCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTTCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCa                                                                                                             >  3:436526/1‑149 (MQ=255)
                                          cGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAgg                                                                                                           <  1:56837/149‑1 (MQ=255)
                                               gTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTc                                                                                                      <  4:113759/149‑1 (MQ=255)
                                               gTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTc                                                                                                      <  4:266737/149‑1 (MQ=255)
                                                                    cAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGTGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAgg                                                                                                                                    <  3:268285/98‑1 (MQ=255)
                                                                    cAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGTGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAgg                                                                                                                                    >  4:268285/1‑98 (MQ=255)
                                                                     aGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTgg                                                                                <  4:614006/149‑1 (MQ=255)
                                                                          ccGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCt                                                                                                                      >  1:540056/1‑106 (MQ=255)
                                                                          ccGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCt                                                                                                                      <  2:540056/106‑1 (MQ=255)
                                                                           cGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcc                                                                          <  2:461798/149‑1 (MQ=255)
                                                                           cGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcc                                                                          <  2:56974/149‑1 (MQ=255)
                                                                            gCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcct                                                                         <  3:586626/149‑1 (MQ=255)
                                                                              cAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcctga                                                                       <  4:478245/149‑1 (MQ=255)
                                                                               aGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcctgat                                                                      <  4:581556/149‑1 (MQ=255)
                                                                                  gcCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcctgatcct                                                                   <  3:507580/149‑1 (MQ=255)
                                                                                   ccGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcctgatcctg                                                                  <  3:446866/149‑1 (MQ=255)
                                                                                    cGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAATGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGCTTatcctgat                                                                      >  1:425080/1‑144 (MQ=255)
                                                                                    cGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcctgat                                                                      <  2:425080/144‑1 (MQ=255)
                                                                                     gAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGtt                                                                <  2:272389/149‑1 (MQ=255)
                                                                                        cAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCAt                                                             <  1:438362/149‑1 (MQ=255)
                                                                                          aCAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTACATAc                                                           <  3:261969/149‑1 (MQ=255)
                                                                                            agcagTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCa                                                         >  1:94449/1‑149 (MQ=255)
                                                                                             gcagTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTg                                                                                 <  3:134219/124‑1 (MQ=255)
                                                                                             gcagTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGGTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTg                                                                                 >  4:134219/1‑124 (MQ=255)
                                                                                                 tAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGtt                                                    <  1:387820/149‑1 (MQ=255)
                                                                                                 tAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGtt                                                    >  1:523138/1‑149 (MQ=255)
                                                                                                 tAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGtt                                                    <  1:338646/149‑1 (MQ=255)
                                                                                                    tCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACCGGTTATCCTGATCCTGTTCATACCACTGTTGcc                                                 >  3:226500/1‑149 (MQ=255)
                                                                                                       tttCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGtt                                                                >  3:502546/1‑131 (MQ=255)
                                                                                                       tttCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGtt                                                                <  4:502546/131‑1 (MQ=255)
                                                                                                       tttCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATa                                                            >  3:529186/1‑135 (MQ=255)
                                                                                                       tttCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATa                                                            <  4:529186/135‑1 (MQ=255)
                                                                                                       tttCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATAc                                                           <  3:207166/136‑1 (MQ=255)
                                                                                                       tttCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATAc                                                           >  4:207166/1‑136 (MQ=255)
                                                                                                       tttCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACt                                                       <  3:129278/140‑1 (MQ=255)
                                                                                                       tttCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACt                                                       >  4:129278/1‑140 (MQ=255)
                                                                                                       tttCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAgc                                              >  1:37023/1‑149 (MQ=255)
                                                                                                         tcatcaCGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAAAGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAgcgc                                            <  3:455610/149‑1 (MQ=255)
                                                                                                           atcaCGTGACGGCGGGTGAGGCGGGTATCGTGCAAAGCCCGGCTGGCAAAGTCGCCGGGCACACCGCCAAGCTGGTTGCGCAGGGTTTCAACCTCCTGCCAGGCCCACAGGTTATCCTTATACTGGTCATAACACTGTTGCCAgcgcgc                                          >  1:233880/1‑149 (MQ=255)
                                                                                                              aCGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTc                                       >  3:81870/1‑149 (MQ=255)
                                                                                                              aCGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTc                                       >  3:621425/1‑149 (MQ=255)
                                                                                                                   aCGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCtgtt                                  <  2:311956/149‑1 (MQ=255)
                                                                                                                     ggcggGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGTCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCtgttgt                                <  4:485062/149‑1 (MQ=255)
                                                                                                                      gcggGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTtgtg                               <  4:536439/149‑1 (MQ=255)
                                                                                                                       cggGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTgg                              <  3:292480/149‑1 (MQ=255)
                                                                                                                        gggTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcct                                                                         >  2:549986/1‑105 (MQ=255)
                                                                                                                        gggTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcct                                                                         <  1:549986/105‑1 (MQ=255)
                                                                                                                         ggTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGc                            <  2:233880/149‑1 (MQ=255)
                                                                                                                             aGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGAc                        <  3:243532/149‑1 (MQ=255)
                                                                                                                             aGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCACCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGAc                        >  2:169699/1‑149 (MQ=255)
                                                                                                                                cGGGTATCGTGCAACGCCCGGCTGGCAACGTCACCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGAcgcg                     <  4:116497/149‑1 (MQ=255)
                                                                                                                                   gTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTc                  <  4:190138/149‑1 (MQ=255)
                                                                                                                                    tATCGTGCAGCGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTcg                 <  2:175404/149‑1 (MQ=255)
                                                                                                                                              cGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCa       >  4:160207/1‑149 (MQ=255)
                                                                                                                                                  cGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGc   <  1:248522/149‑1 (MQ=255)
                                                                                                                                                   ggCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCa  <  4:621425/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCA  >  NC_000913/4517363‑4517658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: