Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,549,932 T→A 6.3% intergenic (+222/+21) fimH → / ← gntP minor component of type 1 fimbriae/fructuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,549,9320TA 6.3% 218.0 / 5.5 63intergenic (+222/+21)fimH/gntPminor component of type 1 fimbriae/fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base T (32/27);  new base A (2/2);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

GCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAG  >  NC_000913/4549787‑4550080
                                                                                                                                                 |                                                                                                                                                    
gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTgg                                                                                                                                                   >  3:225824/1‑149 (MQ=255)
 ccAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGc                                                                                                                                                  >  4:582251/1‑149 (MQ=255)
        aGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctca                                                                                                                                           <  3:582251/149‑2 (MQ=255)
           cgcgTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGgt                                                                                                                                        <  3:576011/149‑1 (MQ=255)
            gcgTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAtt                                                                                                                                                     >  2:444419/1‑135 (MQ=255)
            gcgTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAtt                                                                                                                                                     <  1:444419/135‑1 (MQ=255)
            gcgTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGgtg                                                                                                                                       >  3:56910/1‑149 (MQ=255)
                    gTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTa                                                                                                                               >  3:406706/1‑149 (MQ=255)
                               cATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGc                                                                                                                    >  2:112460/1‑149 (MQ=255)
                                aTAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGctct                                                                                                                                           >  1:251027/1‑125 (MQ=255)
                                aTAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGctct                                                                                                                                           <  2:251027/125‑1 (MQ=255)
                                aTAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCt                                                                                                                   >  1:371656/1‑149 (MQ=255)
                                aTAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCt                                                                                                                   >  2:200276/1‑149 (MQ=255)
                                   aaCAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCTTTTTACGCTACCATGCtaat                                                                                                                >  3:93480/1‑149 (MQ=255)
                                    aCAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCtaata                                                                                                               >  1:222497/1‑149 (MQ=255)
                                       aaGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATc                                                                                                            <  4:100390/149‑1 (MQ=255)
                                                     ggTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAg                                                                                              <  3:452966/149‑1 (MQ=255)
                                                       tCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaataat                                                                                                 >  1:412616/1‑144 (MQ=255)
                                                       tCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaataat                                                                                                 <  2:412616/144‑1 (MQ=255)
                                                       tCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGcc                                                                                            <  1:112460/149‑1 (MQ=255)
                                                          gTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaaaaa                                                                                                  >  4:290968/1‑137 (MQ=255)
                                                          gTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaaaaa                                                                                                  <  3:290968/140‑1 (MQ=255)
                                                          gTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGc                                                                                             <  1:462575/145‑1 (MQ=255)
                                                          gTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGc                                                                                             >  2:462575/1‑145 (MQ=255)
                                                               ccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACg                                                                                    >  3:199261/1‑149 (MQ=255)
                                                               ccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACg                                                                                    >  2:559988/1‑149 (MQ=255)
                                                               ccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACg                                                                                    >  1:97949/1‑149 (MQ=255)
                                                                cTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTg                                                                                                                                          >  4:566721/1‑94 (MQ=255)
                                                                cTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTg                                                                                                                                          <  3:566721/94‑1 (MQ=255)
                                                                  cACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAg                                                                                 >  1:288470/1‑149 (MQ=255)
                                                                   aCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCt                                                                                                                   <  2:366869/114‑1 (MQ=255)
                                                                   aCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCt                                                                                                                   >  1:366869/1‑114 (MQ=255)
                                                                       tAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGAc                                                                            <  4:284488/149‑1 (MQ=255)
                                                                       tAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGAc                                                                            <  4:56910/149‑1 (MQ=255)
                                                                            ctctCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCt                                                                       >  3:396548/1‑149 (MQ=255)
                                                                             tctcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTc                                                                      >  2:466708/1‑149 (MQ=255)
                                                                              ctcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTcc                                                                     >  2:32737/1‑149 (MQ=255)
                                                                                 cccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCcagc                                                                  >  2:16273/1‑149 (MQ=255)
                                                                                  ccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCcagca                                                                 >  3:52282/1‑149 (MQ=255)
                                                                                    ggAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGa                                                               <  4:225824/149‑1 (MQ=255)
                                                                                          gCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAg                                                         <  4:406706/149‑1 (MQ=255)
                                                                                              ggggACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGttt                                                     <  2:371656/149‑1 (MQ=255)
                                                                                                  aCTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCaa                                                 >  3:386245/1‑149 (MQ=255)
                                                                                                       ccGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACgtgt                                            <  1:466708/149‑1 (MQ=255)
                                                                                                             acaTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCTTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTa                                      <  4:93480/149‑1 (MQ=255)
                                                                                                             acaTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTa                                      <  4:386245/149‑1 (MQ=255)
                                                                                                               aTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGAc                                                              >  1:130162/1‑123 (MQ=255)
                                                                                                               aTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGAc                                                              <  2:130162/123‑1 (MQ=255)
                                                                                                                tttttAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTg                                   >  1:69423/1‑149 (MQ=255)
                                                                                                                 ttttAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGa                                  <  2:69423/149‑1 (MQ=255)
                                                                                                                      gACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGt                             >  4:508947/1‑149 (MQ=255)
                                                                                                                      gACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGt                             <  2:407099/149‑1 (MQ=255)
                                                                                                                               atcaGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAAc                    <  1:32737/149‑1 (MQ=255)
                                                                                                                                tcaGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAAcc                   >  3:133049/1‑149 (MQ=255)
                                                                                                                                   gTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCttt                <  3:508947/149‑1 (MQ=255)
                                                                                                                                   gTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCttt                <  4:133049/149‑1 (MQ=255)
                                                                                                                                     cTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGa              <  4:396548/149‑1 (MQ=255)
                                                                                                                                       gAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAg                        <  4:55020/137‑1 (MQ=255)
                                                                                                                                       gAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAg                        >  3:55020/1‑137 (MQ=255)
                                                                                                                                          ccTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGaacaac         >  1:614089/1‑149 (MQ=255)
                                                                                                                                           cTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAAcc        <  1:559988/149‑1 (MQ=255)
                                                                                                                                           cTGCCATTGGCAGGCTCTGGCGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAAcc        >  1:372914/1‑149 (MQ=255)
                                                                                                                                            tGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCa       >  2:399440/1‑149 (MQ=255)
                                                                                                                                                aTTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGaaa   <  2:222497/149‑1 (MQ=255)
                                                                                                                                                aTTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGaaa   >  4:399840/1‑149 (MQ=255)
                                                                                                                                                 ttGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAg  >  4:614868/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAG  >  NC_000913/4549787‑4550080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑