Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 489,958 T→C 18.5% intergenic (+60/+152) mscK → / ← ybaM mechanosensitive channel protein, intermediate conductance, K+ regulated/DUF2496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913489,9580TC18.5% 57.8 / 8.7 27intergenic (+60/+152)mscK/ybaMmechanosensitive channel protein, intermediate conductance, K+ regulated/DUF2496 family protein
Reads supporting (aligned to +/- strand):  ref base T (6/16);  new base C (2/3);  total (8/19)
Fisher's exact test for biased strand distribution p-value = 6.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

GCACAACGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGCCCGCAACCTTAGGGGACATTTAGCGACCCC  >  NC_000913/489820‑490100
                                                                                                                                          |                                                                                                                                              
gCACAACGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCAtccg                                                                                                                                      >  1:379273/1‑146 (MQ=255)
 cacaACGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATgcgg                                                                                                                                     <  1:73447/149‑1 (MQ=255)
    aaCGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATccggcat                                                                                                                                  <  3:520747/149‑3 (MQ=255)
     aCGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATg                                                                                                                                 <  2:379273/149‑1 (MQ=255)
       gagaAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGAc                                                                                                                               <  2:347021/149‑1 (MQ=255)
             ggCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCtt                                                                                                                         >  4:474669/1‑149 (MQ=255)
                      gTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCACCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCt                                                                                                                >  4:396267/1‑149 (MQ=255)
                           ggAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa                                                                                                                               >  4:266568/1‑128 (MQ=255)
                           ggAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa                                                                                                                               <  3:266568/129‑2 (MQ=255)
                            gAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGaaa                                                                                                          <  2:145531/149‑1 (MQ=255)
                                tAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGc                                                                                                      >  2:270871/1‑149 (MQ=255)
                                  aaaCGCGACTACAAAGGCGATGACCCGAGGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCaa                                                                                                    <  3:98082/149‑1 (MQ=255)
                                  aaaCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCaa                                                                                                    <  4:174618/149‑1 (MQ=255)
                                          cTACAAAGGCGATGACCCGAAGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCTCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa                                                                                            <  1:42555/149‑1 (MQ=255)
                                                  gCGATGACCCGACGCCAGCAGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGc                                                                                    <  1:270871/149‑1 (MQ=255)
                                                       gACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAg                                                                               <  3:135256/149‑1 (MQ=255)
                                                       gACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAg                                                                               <  1:482965/149‑1 (MQ=255)
                                                                 cAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGc                                                                     <  2:399323/149‑1 (MQ=255)
                                                                  aGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGcc                                                                    <  3:326039/149‑1 (MQ=255)
                                                                          ggTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGc                                                            <  3:243251/149‑1 (MQ=255)
                                                                                 aCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCATGAGCGGTGTAGGCCTGATAAGCGTAgcgc                                                     <  3:396267/149‑1 (MQ=255)
                                                                                                    aGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGttt                                  <  1:328942/149‑1 (MQ=255)
                                                                                                          cccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGCCCGc                            >  3:192946/1‑149 (MQ=255)
                                                                                                           ccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCaa                                                                                                    >  3:452728/1‑76 (MQ=255)
                                                                                                           ccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCaa                                                                                                    <  4:452728/76‑1 (MQ=255)
                                                                                                                               cgcgTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGCCCGCAACCTTAGGGGACATTTAGCg       >  1:213014/1‑149 (MQ=255)
                                                                                                                                    tgttCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGCCCGCAACCTTAGGGGACATTTAGCGAcccc  <  3:474669/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                              
GCACAACGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGCCCGCAACCTTAGGGGACATTTAGCGACCCC  >  NC_000913/489820‑490100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: