Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 744,702 A→C 6.0% K154Q (AAA→CAA)  ybgK → putative allophanate hydrolase, subunit 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913744,7020AC 6.0% 225.1 / 5.1 66K154Q (AAA→CAA) ybgKputative allophanate hydrolase, subunit 2
Reads supporting (aligned to +/- strand):  ref base A (33/29);  new base C (2/2);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCA  >  NC_000913/744557‑744850
                                                                                                                                                 |                                                                                                                                                    
gcagcaCGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAAt                                                                                                                                                   >  1:190281/1‑149 (MQ=255)
gcagcaCGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAAt                                                                                                                                                   <  2:190281/149‑1 (MQ=255)
 cagcaCGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATc                                                                                                                                                  <  1:250054/149‑1 (MQ=255)
 cagcaCGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCACCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATc                                                                                                                                                  <  1:47139/149‑1 (MQ=255)
    caCGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGaa                                                                                                                                               <  1:614621/149‑1 (MQ=255)
      cGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTGCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGc                                                                                                                                             <  1:508970/149‑1 (MQ=255)
          aTGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGa                                                                                                                                         <  4:496843/149‑1 (MQ=255)
             cgcAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTc                                                                                                                                      <  2:27723/149‑1 (MQ=255)
              gcAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCt                                                                                                                                     >  4:172808/1‑149 (MQ=255)
              gcAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCt                                                                                                                                     <  4:509110/149‑1 (MQ=255)
               cAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTa                                                                                                                                    <  2:229832/149‑1 (MQ=255)
                        ggCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTTGCCAATCggga                                                                                                                                              <  1:225147/130‑4 (MQ=255)
                        ggCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTTGCCAATCggga                                                                                                                                              >  2:225147/1‑127 (MQ=255)
                                cgGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGtt                                                                                                                   <  3:172808/149‑1 (MQ=255)
                                       tATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAgc                                                                                                            <  1:436451/149‑1 (MQ=255)
                                        aTTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAgct                                                                                                           >  4:210062/1‑149 (MQ=255)
                                        aTTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGCGCTTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAgct                                                                                                           >  1:210806/1‑149 (MQ=255)
                                           gATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAgctgct                                                                                                        >  3:123328/1‑149 (MQ=255)
                                           gATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAgctgct                                                                                                        <  2:549456/149‑1 (MQ=255)
                                            aTGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGctgctg                                                                                                       >  3:609627/1‑149 (MQ=255)
                                              gTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCtgtg                                                                                                     <  1:521285/149‑1 (MQ=255)
                                               ttCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTTGCCAATCggga                                                                                                                                              <  2:196181/107‑4 (MQ=255)
                                               ttCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTTGCCAATCggga                                                                                                                                              >  1:196181/1‑104 (MQ=255)
                                               ttCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTgg                                                                                                    <  1:498457/149‑1 (MQ=255)
                                               ttCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTgg                                                                                                    >  4:541201/1‑149 (MQ=255)
                                                tCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTggg                                                                                                   <  2:256416/149‑1 (MQ=255)
                                                tCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTggg                                                                                                   <  4:14940/149‑1 (MQ=255)
                                                tCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTggg                                                                                                   <  2:346633/149‑1 (MQ=255)
                                                            gggCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTc                                                                                       >  4:293024/1‑149 (MQ=255)
                                                              gCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATGGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTcgc                                                                                     >  2:202475/1‑149 (MQ=255)
                                                               cTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTcgcg                                                                                    >  3:554488/1‑149 (MQ=255)
                                                                       gcaCCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCg                                                                            >  1:291314/1‑149 (MQ=255)
                                                                       gcaCCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCg                                                                            <  3:158860/149‑1 (MQ=255)
                                                                         aCCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCggg                                                                          <  2:286483/149‑1 (MQ=255)
                                                                            gATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGcc                                                                       >  4:190970/1‑149 (MQ=255)
                                                                            gATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGcc                                                                       >  4:330281/1‑149 (MQ=255)
                                                                              tctcAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCgg                                                                     >  4:568767/1‑149 (MQ=255)
                                                                                tcAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGaa                                                                   >  2:419687/1‑149 (MQ=255)
                                                                                tcAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGaa                                                                   <  4:242595/149‑1 (MQ=255)
                                                                                tcAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGaa                                                                   >  3:581751/1‑149 (MQ=255)
                                                                                tcAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGaa                                                                   >  1:317520/1‑149 (MQ=255)
                                                                                 cAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAat                                                                  >  1:471703/1‑149 (MQ=255)
                                                                                  aaaGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAata                                                                 >  3:382347/1‑149 (MQ=255)
                                                                                   aaGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAatat                                                                <  4:80481/149‑1 (MQ=255)
                                                                                        gggATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTGCCGAATGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGa                                                           <  1:72306/149‑1 (MQ=255)
                                                                                        gggATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATc                                                               >  3:560900/1‑145 (MQ=255)
                                                                                        gggATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATc                                                               <  4:560900/145‑1 (MQ=255)
                                                                                        gggATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGa                                                           >  4:597602/1‑149 (MQ=255)
                                                                                           aTTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTAGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGtt                                                        >  3:390799/1‑149 (MQ=255)
                                                                                                   gCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGACGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATcgcg                                                >  4:223419/1‑149 (MQ=255)
                                                                                                                    tGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTc                               >  2:196215/1‑149 (MQ=255)
                                                                                                                    tGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGAGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTc                               >  4:27995/1‑149 (MQ=255)
                                                                                                                     gAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCt                              <  4:581751/149‑1 (MQ=255)
                                                                                                                           tGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTACACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCgat                        >  1:487923/1‑147 (MQ=255)
                                                                                                                           tGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGtt                        >  2:546768/1‑149 (MQ=255)
                                                                                                                               gACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGcc                    >  1:114526/1‑149 (MQ=255)
                                                                                                                                  cGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTg                 >  4:56517/1‑149 (MQ=255)
                                                                                                                                  cGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTg                 <  1:196175/149‑1 (MQ=255)
                                                                                                                                      tCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAg             >  1:266423/1‑149 (MQ=255)
                                                                                                                                      tCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAg             >  3:274576/1‑149 (MQ=255)
                                                                                                                                         cGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTc                                                       <  3:605704/104‑1 (MQ=255)
                                                                                                                                         cGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTc                                                       >  4:605704/1‑104 (MQ=255)
                                                                                                                                          gATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTa         <  3:210062/149‑1 (MQ=255)
                                                                                                                                              ggCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTc     <  2:266423/149‑1 (MQ=255)
                                                                                                                                               gCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCa                                                              >  1:363608/1‑91 (MQ=255)
                                                                                                                                               gCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCa                                                              <  2:363608/91‑1 (MQ=255)
                                                                                                                                                 aaaTCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCa  >  2:142400/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCA  >  NC_000913/744557‑744850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: