Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,194,657 Δ1 bp 5.4% coding (295/654 nt) rluE ← 23S rRNA pseudouridine(2457) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,194,6570T. 5.4% 409.4 / 15.0 111coding (295/654 nt)rluE23S rRNA pseudouridine(2457) synthase
Reads supporting (aligned to +/- strand):  ref base T (56/49);  new base . (3/3);  total (59/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 118 total aligned reads displayed.

CGGGTTCGTCAACCAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACA  >  NC_000913/1194510‑1194805
                                                                                                                                                    |                                                                                                                                                    
    ttCGTCAACCAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAAc                                                                                                                                                 <  1:1537967/149‑1 (MQ=255)
      cGTCAACCAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAAcgc                                                                                                                                               <  2:1150216/149‑1 (MQ=255)
            ccAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAAc                                                                                                                                                 <  2:603116/141‑1 (MQ=255)
            ccAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGc                                                                                                                                         >  1:1525126/1‑149 (MQ=255)
                      gcCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGt                                                                                                                               <  4:399379/149‑1 (MQ=255)
                               cAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCa                                                                                                                      <  2:191085/149‑1 (MQ=255)
                                       ggCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGc                                                                                                              >  2:1834807/1‑149 (MQ=255)
                                       ggCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGc                                                                                                              >  4:856616/1‑149 (MQ=255)
                                         ccATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa                                                                                                            <  2:1525126/149‑1 (MQ=255)
                                         ccATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGCGCGTTTAACCCAGCCGGGTAAAcgcac                                                                                                                                             >  1:401592/1‑114 (MQ=38)
                                         ccATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGCGCGTTTAACCCAGCCGGGTAAAcgcac                                                                                                                                             <  2:401592/116‑3 (MQ=38)
                                          catcatTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTaaa                                                                                                                                                  <  2:1789542/110‑1 (MQ=255)
                                          catcatTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTaaa                                                                                                                                                  >  1:1789542/1‑110 (MQ=255)
                                           atcatTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAAcc                                                                                                          >  2:1096953/1‑149 (MQ=255)
                                               tttAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTc                                                                                                      <  2:1740276/149‑1 (MQ=255)
                                                ttAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCg                                                                                                     <  3:856616/149‑1 (MQ=255)
                                                     gTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATc                                                                                                <  1:293834/149‑1 (MQ=255)
                                                           ccATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTc                                                                                          >  3:1879871/1‑149 (MQ=255)
                                                           ccATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTc                                                                                          >  1:663806/1‑149 (MQ=255)
                                                            cATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCAc                                                                                                                  <  1:1296891/124‑1 (MQ=255)
                                                             aTTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCaa                                                                                        >  4:1351107/1‑149 (MQ=255)
                                                             aTTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCaa                                                                                        >  3:891020/1‑149 (MQ=255)
                                                              ttGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAg                                                                                       <  4:1722934/149‑1 (MQ=255)
                                                                  gcAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGa                                                                                   >  2:598930/1‑149 (MQ=255)
                                                                    aaGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGt                                                                                                                        >  2:389540/1‑110 (MQ=255)
                                                                    aaGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGAcc                                                                                 >  4:1113806/1‑149 (MQ=255)
                                                                       gCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACctgc                                                                              >  3:921614/1‑149 (MQ=255)
                                                                        cTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACctgct                                                                             <  4:891020/149‑1 (MQ=255)
                                                                         ttCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAAc                                                                                                                                                 <  4:882589/80‑1 (MQ=255)
                                                                         ttCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGt                                                                                                                               >  3:1007164/1‑98 (MQ=255)
                                                                         ttCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGt                                                                                                                               <  4:1007164/98‑1 (MQ=255)
                                                                              gTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTg                                                                                                                          <  3:583375/98‑1 (MQ=255)
                                                                                gCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAac                                                                     <  4:1879871/149‑1 (MQ=255)
                                                                                  gTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTCCCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAacac                                                                   <  2:1458612/149‑1 (MQ=255)
                                                                                   tCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGtt                                                                                                                                                     >  4:1851577/1‑66 (MQ=255)
                                                                                   tCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGtt                                                                                                                                                     <  2:1712998/66‑1 (MQ=255)
                                                                                   tCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGtt                                                                                                                                                     >  1:1712998/1‑66 (MQ=255)
                                                                                   tCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGtt                                                                                                                                                     <  3:1851577/66‑1 (MQ=255)
                                                                                    cTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACAccc                                                                 <  4:921614/149‑1 (MQ=255)
                                                                                     ttGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATc                                                                                                <  2:1702679/117‑1 (MQ=255)
                                                                                     ttGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATc                                                                                                >  1:1702679/1‑117 (MQ=255)
                                                                                     ttGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCt                                                                <  3:1060702/149‑1 (MQ=255)
                                                                                        tgtgGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGaa                                                             >  1:531479/1‑149 (MQ=255)
                                                                                            ggAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCgg                                                         <  1:598930/149‑1 (MQ=255)
                                                                                                tACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATg                                                     >  3:1713615/1‑149 (MQ=255)
                                                                                                tACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATg                                                     >  3:1784055/1‑149 (MQ=255)
                                                                                                 aCCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGa                                                    >  1:142788/1‑149 (MQ=255)
                                                                                                  ccTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGaa                                                   <  3:1113806/149‑1 (MQ=255)
                                                                                                   cTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCACCGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAAt                                                  >  3:787331/1‑149 (MQ=255)
                                                                                                   cTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCAc                                                                                                                  >  4:563108/1‑85 (MQ=255)
                                                                                                   cTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCAc                                                                                                                  <  3:563108/85‑1 (MQ=255)
                                                                                                   cTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAg                                                                                                               <  1:781254/88‑1 (MQ=255)
                                                                                                   cTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAg                                                                                                               >  2:781254/1‑88 (MQ=255)
                                                                                                   cTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATcgc                                                                                              >  3:942888/1‑105 (MQ=255)
                                                                                                   cTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACAccc                                                                 >  1:794407/1‑134 (MQ=255)
                                                                                                    ttCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCa                                                                                                                      >  2:1081042/1‑80 (MQ=255)
                                                                                                    ttCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAAtt                                                 >  1:150015/1‑149 (MQ=255)
                                                                                                    ttCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAAtt                                                 >  3:1824672/1‑149 (MQ=255)
                                                                                                    ttCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAAtt                                                 >  1:639625/1‑149 (MQ=255)
                                                                                                    ttCCACCTGCACATAATAGATTTTTCCGGCGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCa                                                                                                                      <  1:1081042/80‑1 (MQ=255)
                                                                                                       cACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAg                                                                                       >  1:1154450/1‑108 (MQ=255)
                                                                                                       cACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAg                                                                                       <  2:1154450/108‑1 (MQ=255)
                                                                                                       cACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCtt                                              <  2:531479/149‑1 (MQ=255)
                                                                                                        aCCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCttt                                             <  1:1834807/149‑1 (MQ=255)
                                                                                                        aCCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCttt                                             <  2:150015/149‑1 (MQ=255)
                                                                                                          cTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTa                                           >  2:750255/1‑149 (MQ=255)
                                                                                                                ataataGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCg                                                          <  3:1769068/128‑1 (MQ=255)
                                                                                                                 taataGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGtttt                                    <  4:1713615/149‑1 (MQ=255)
                                                                                                                 taataGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGtttt                                    <  2:142788/149‑1 (MQ=255)
                                                                                                                 taataGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGtttt                                    <  2:663806/149‑1 (MQ=255)
                                                                                                                   ataGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCa                                                                                                                   <  2:892946/68‑1 (MQ=255)
                                                                                                                   ataGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACAccc                                                                 >  3:1203783/1‑118 (MQ=255)
                                                                                                                   ataGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACAccc                                                                 <  4:1203783/118‑1 (MQ=255)
                                                                                                                   ataGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCtt                                              <  1:1272756/137‑1 (MQ=255)
                                                                                                                   ataGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCtt                                              >  2:1272756/1‑137 (MQ=255)
                                                                                                                   ataGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGc                                  <  1:1096953/149‑1 (MQ=255)
                                                                                                                    taGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGtttt                                    >  1:251173/1‑146 (MQ=255)
                                                                                                                       aTTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTcc                              >  3:1386713/1‑149 (MQ=255)
                                                                                                                        tttttCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGtt                                      <  3:915649/140‑1 (MQ=255)
                                                                                                                        tttttCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGtt                                      >  4:915649/1‑140 (MQ=255)
                                                                                                                                gcgCGTTTAACCCAGCCGGGTAAACGCACCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCtt                                              >  4:1013098/3‑124 (MQ=38)
                                                                                                                                gTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATc                     >  3:1519765/1‑149 (MQ=255)
                                                                                                                                   cGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGt                  <  3:1351107/149‑1 (MQ=255)
                                                                                                                                    gtttaaCCCAGCCGGGTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCtt                                              >  2:1478167/6‑120 (MQ=255)
                                                                                                                                    gtttaaCCCAGCCGGGTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCtt                                              <  1:1478167/115‑1 (MQ=255)
                                                                                                                                    gTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCttt                                             >  3:499263/1‑121 (MQ=255)
                                                                                                                                    gTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCttt                                             >  1:976144/1‑121 (MQ=255)
                                                                                                                                    gTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCttt                                             <  2:976144/121‑1 (MQ=255)
                                                                                                                                    gTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCttt                                             <  4:499263/121‑1 (MQ=255)
                                                                                                                                     tttACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTcc                              >  2:450984/1‑135 (MQ=255)
                                                                                                                                     tttACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTcc                              <  1:476302/135‑1 (MQ=255)
                                                                                                                                     tttACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTcc                              <  1:450984/135‑1 (MQ=255)
                                                                                                                                        aaCCCAGCCGGGTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAAt                                         <  4:47522/120‑1 (MQ=255)
                                                                                                                                           ccGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGc           >  3:1602564/1‑149 (MQ=255)
                                                                                                                                             ggCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCgg         >  3:188973/1‑149 (MQ=255)
                                                                                                                                                 gggTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGgaaga     >  2:464095/1‑149 (MQ=255)
                                                                                                                                                 gggTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGgaaga     >  1:1832013/1‑149 (MQ=255)
                                                                                                                                                   gTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAAc   >  1:511145/1‑149 (MQ=255)
                                                                                                                                                    ttAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACa  >  1:834101/1‑149 (MQ=255)
                                                                                                                                                    ttAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACa  >  2:324996/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGGTTCGTCAACCAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACA  >  NC_000913/1194510‑1194805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: