Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,622,354	Δ1 bp	6.0%	coding (121/639 nt)	purN →	phosphoribosylglycinamide formyltransferase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,622,354	0	T	.	6.0%	242.6 / 8.4	67	coding (121/639 nt)	purN	phosphoribosylglycinamide formyltransferase 1
Reads supporting (aligned to +/- strand):  ref base T (36/27);  new base . (2/2);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

GAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTT  >  NC_000913/2622211‑2622495                                                                                                                                                 |                                                                                                                                              gAACAACGCGTGGTTATCGAATAATGTATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGc                                                                                                                                          <  1:925940/149‑1 (MQ=255)                         tGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCtt                                                                                                                                       >  1:548890/1‑128 (MQ=255)                         tGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAAGTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCtt                                                                                                                                       <  2:548890/128‑1 (MQ=255)                                           tttCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAAcgcg                                                                                                                                >  4:1712202/1‑117 (MQ=255)                                           tttCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAAcgcg                                                                                                                                <  3:1712202/117‑1 (MQ=255)                                                    aCGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAgc                                                                                       >  2:1207692/1‑149 (MQ=255)                                                             aTTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGc                                                                                                                      >  2:847149/1‑109 (MQ=255)                                                             aTTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGc                                                                                                                      <  1:847149/109‑1 (MQ=255)                                                              tttACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGAc                                                                              <  3:1526225/148‑1 (MQ=255)                                                              tttACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGAc                                                                              >  4:1526225/1‑148 (MQ=255)                                                                  cAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCg                                                                         >  4:1093092/1‑149 (MQ=255)                                                                     gCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTAt                                                                                                                <  3:1705828/107‑1 (MQ=255)                                                                     gCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTAt                                                                                                                >  4:1705828/1‑107 (MQ=255)                                                                     gCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGa                                                                      >  1:845077/1‑149 (MQ=255)                                                                                  ccTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgg                                                         <  3:1093092/149‑1 (MQ=255)                                                                                   cTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTg                                                                                                                                      >  4:1290096/1‑71 (MQ=255)                                                                                   cTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCCTCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCggg                                                        >  1:469972/1‑149 (MQ=255)                                                                                   cTGTAAAACCAACAAAATTAAAGCCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTg                                                                                                                                      <  3:1290096/71‑1 (MQ=255)                                                                                     gTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACg                                                                                                   <  1:949054/104‑1 (MQ=255)                                                                                     gTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACg                                                                                                   >  2:949054/1‑104 (MQ=255)                                                                                      tAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGt                                                     >  3:319807/1‑149 (MQ=255)                                                                                        aaaCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgg                                                         >  2:1654923/1‑143 (MQ=255)                                                                                        aaaCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgg                                                         <  1:1654923/143‑1 (MQ=255)                                                                                         aaCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGa                                                  <  4:319807/149‑1 (MQ=255)                                                                                             aacaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCa                                              >  4:1649821/1‑149 (MQ=255)                                                                                             aacaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCa                                              >  2:203030/1‑149 (MQ=255)                                                                                             aacaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCa                                              >  4:1254956/1‑149 (MQ=255)                                                                                             aacaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCa                                              >  4:454353/1‑149 (MQ=255)                                                                                              acaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAAc                                                                                                          >  3:204879/1‑88 (MQ=255)                                                                                              acaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAAc                                                                                                          <  4:204879/88‑1 (MQ=255)                                                                                              acaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGa                                                       <  4:1466533/139‑1 (MQ=255)                                                                                              acaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGa                                                       >  3:1466533/1‑139 (MQ=255)                                                                                              acaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAg                                                      >  1:1348778/1‑140 (MQ=255)                                                                                              acaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAg                                                      <  2:1348778/140‑1 (MQ=255)                                                                                                aaaaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGt                                                     >  4:1580958/1‑139 (MQ=255)                                                                                                aaaaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGt                                                     <  3:1580958/139‑1 (MQ=255)                                                                                                  aaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCCTCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGaaa                                         <  2:469972/149‑1 (MQ=255)                                                                                                  aaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGa                                                  <  2:1193890/140‑1 (MQ=255)                                                                                                  aaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGa                                                  >  1:1193890/1‑140 (MQ=255)                                                                                                   aTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGtt                                                    <  4:282161/137‑1 (MQ=255)                                                                                                   aTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGtt                                                    >  3:282161/1‑137 (MQ=255)                                                                                                          gCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgg                                                         <  4:926145/125‑1 (MQ=255)                                                                                                          gCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgg                                                         >  3:926145/1‑125 (MQ=255)                                                                                                          gCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATg                                 >  4:1080270/1‑149 (MQ=255)                                                                                                                    ggCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTa                                                                <  2:910145/108‑1 (MQ=255)                                                                                                                    ggCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTa                                                                >  1:910145/1‑108 (MQ=255)                                                                                                                    ggCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCg                       >  3:1100242/1‑149 (MQ=255)                                                                                                                     gCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGa                      >  1:477180/1‑149 (MQ=255)                                                                                                                      cAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCAccc                        >  3:88468/1‑146 (MQ=255)                                                                                                                      cAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCAccc                        <  4:88468/146‑1 (MQ=255)                                                                                                                        gTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAtgt                   >  1:312601/1‑149 (MQ=255)                                                                                                                         ttttCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAtgtg                  >  2:341168/1‑149 (MQ=255)                                                                                                                         ttttCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAtgtg                  >  1:1348193/1‑149 (MQ=255)                                                                                                                         ttttCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAtgtg                  >  2:215872/1‑149 (MQ=255)                                                                                                                              aGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTc                                                                          >  1:426438/1‑88 (MQ=255)                                                                                                                              aGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTc                                                                          <  2:426438/88‑1 (MQ=255)                                                                                                                              aGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGt             <  1:341168/149‑1 (MQ=255)                                                                                                                                    aaGGCCGAACGCG‑TCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTa                               <  3:485251/125‑1 (MQ=255)                                                                                                                                    aaGGCCGAACGCG‑TCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTa                               >  1:1751392/1‑125 (MQ=255)                                                                                                                                    aaGGCCGAACGCG‑TCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTa                               >  4:485251/1‑125 (MQ=255)                                                                                                                                    aaGGCCGAACGCG‑TCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTa                               <  2:1751392/125‑1 (MQ=255)                                                                                                                                      ggCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTAc                              >  4:947031/1‑124 (MQ=255)                                                                                                                                      ggCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTAc                              <  3:947031/124‑1 (MQ=255)                                                                                                                                      ggCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgctggctg     >  1:1268034/1‑149 (MQ=255)                                                                                                                                      ggCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgctggctg     <  1:1207692/149‑1 (MQ=255)                                                                                                                                       gCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATTAAATCGACATGTACGCACCCGATGTGGTCGTGctggctgg    <  4:1100242/149‑1 (MQ=255)                                                                                                                                         cG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGtt  <  2:845077/149‑1 (MQ=255)                                                                                                                                                 ttCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTa                                                                <  2:69014/80‑1 (MQ=255)                                                                                                                                                 ttCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTa                                                                >  1:69014/1‑80 (MQ=255)                                                                                                                                                 |                                                                                                                                              GAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTT  >  NC_000913/2622211‑2622495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑