Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,706,134 | Δ1 bp | 9.9% | coding (991/1800 nt) | lepA ← | back‑translocating elongation factor EF4, GTPase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,706,134 | 0 | A | . | 9.9% | 490.8 / 46.0 | 142 | coding (991/1800 nt) | lepA | back‑translocating elongation factor EF4, GTPase |
Reads supporting (aligned to +/- strand): ref base A (59/69); new base . (3/11); total (62/80) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 9.36e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01 |
Only 100 of 146 total aligned reads displayed.
TCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCT > NC_000913/2705986‑2706282 | tctcTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGa > 2:727163/1‑149 (MQ=255) tctcTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGa < 4:1004569/149‑1 (MQ=255) tctcTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGa < 1:1879584/149‑1 (MQ=255) ctTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCt < 4:647548/149‑1 (MQ=255) ttGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCtt > 4:1488805/1‑149 (MQ=255) tGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCttt < 2:127539/149‑1 (MQ=255) tGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCttt < 4:1839401/149‑1 (MQ=255) tGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCttt < 1:572342/149‑1 (MQ=255) gACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttg < 2:977124/149‑2 (MQ=255) gACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttg < 3:1125509/149‑2 (MQ=255) aCGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttgg < 1:1620603/149‑3 (MQ=255) cGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttggt < 2:1099725/149‑4 (MQ=255) gTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCgg > 2:1769741/1‑149 (MQ=255) ggTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCt < 4:1121426/149‑1 (MQ=255) tttCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCa < 4:439621/149‑1 (MQ=255) tttCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCa > 2:1155676/1‑149 (MQ=255) ttCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa > 1:335959/1‑149 (MQ=255) cATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGt < 1:1769741/149‑1 (MQ=255) aTACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCttt > 1:1353875/1‑131 (MQ=255) tACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGa > 4:1680935/1‑149 (MQ=255) acacTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAg < 4:1554885/149‑1 (MQ=255) acacTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAg < 3:444090/149‑1 (MQ=255) acTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACGAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa < 4:1565607/142‑1 (MQ=255) acTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttg < 4:124715/128‑2 (MQ=255) acTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttg < 2:1268668/128‑2 (MQ=255) acTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttg > 3:124715/1‑127 (MQ=255) acTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttg > 1:1268668/1‑127 (MQ=255) acTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa > 3:1565607/1‑142 (MQ=255) tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCAt > 2:1750521/1‑149 (MQ=255) ggCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttggtttc < 3:1820597/124‑7 (MQ=255) ggCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAg < 4:1869384/149‑1 (MQ=255) ggCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCAGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAg > 4:703220/1‑149 (MQ=255) gcgcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAgg > 3:1367248/1‑149 (MQ=255) gcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCGTAGAACAGTGAGGCATCGTTCAGGCt > 4:96351/1‑149 (MQ=255) cAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTg > 1:558686/1‑149 (MQ=255) aGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGa < 3:1488805/149‑1 (MQ=255) aGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCAGCGTTCAGGCTGa < 3:505680/149‑1 (MQ=255) tggtgATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGt < 2:558686/149‑1 (MQ=255) gATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCa > 1:1444344/1‑137 (MQ=255) gATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCa < 2:1444344/137‑1 (MQ=255) tCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGaa < 1:112990/118‑1 (MQ=255) agatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAg > 1:1235211/1‑149 (MQ=255) gatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTc > 4:1269708/1‑131 (MQ=255) gatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgc > 3:353639/1‑149 (MQ=255) gatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgc < 3:27207/149‑1 (MQ=255) atcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGAAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCAc > 1:104642/1‑149 (MQ=255) gTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGa < 1:1155676/149‑1 (MQ=255) tATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGaa < 1:1750521/149‑1 (MQ=255) tATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGaa < 3:1680935/149‑1 (MQ=255) ttCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgc < 2:570883/136‑1 (MQ=255) ttCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGaatg > 1:928794/1‑147 (MQ=255) ttCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAgg < 2:1184701/149‑1 (MQ=255) cACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCt < 3:703220/149‑1 (MQ=255) aCGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa < 2:1543187/101‑1 (MQ=255) aCGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgcg > 3:404792/1‑134 (MQ=255) aCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAgtcgt > 1:814275/1‑149 (MQ=255) tGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACt > 3:1770929/1‑149 (MQ=255) ggATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGt < 4:1577877/86‑1 (MQ=255) ggATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACtg > 1:1664678/1‑148 (MQ=255) gatgatCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATa < 1:624835/78‑1 (MQ=255) gatgatCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTa > 3:882056/1‑149 (MQ=255) gatCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCg < 4:353639/149‑1 (MQ=255) gatCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCg > 4:1678240/1‑149 (MQ=255) tctcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCCTCATAGTCGTCGGAACTTACCggg > 3:99353/1‑149 (MQ=255) tcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCa < 3:1555259/91‑1 (MQ=255) tcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGaa < 2:744309/149‑1 (MQ=255) tgtgCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTcc < 2:320761/59‑1 (MQ=255) gtgCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGAc > 1:1524169/1‑149 (MQ=255) gtgCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGAc < 2:1664678/149‑1 (MQ=255) gtgCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGAc < 1:821395/149‑1 (MQ=255) agcagGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGc > 1:31764/1‑149 (MQ=255) agcagGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGAAACTTACCGGGAACAGACCGGc < 2:104642/149‑1 (MQ=255) gcagGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCg > 2:1609313/1‑149 (MQ=255) ccGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATAc > 4:443382/1‑149 (MQ=255) aGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTg > 4:923358/1‑149 (MQ=255) ggAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGc < 3:33051/149‑1 (MQ=255) gAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCg < 3:923358/149‑1 (MQ=255) ccGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGttt < 3:927689/149‑1 (MQ=255) cGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTg > 3:340441/1‑142 (MQ=255) cGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTg < 4:340441/142‑1 (MQ=255) cAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGAc < 4:882056/149‑1 (MQ=255) aGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACt < 2:31764/149‑1 (MQ=255) ggAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTACATAGTCGTCGGAACTTACCGGGAACAGACCGGCGta < 1:1684242/130‑1 (MQ=255) ggAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTACATAGTCGTCGGAACTTACCGGGAACAGACCGGCGta > 2:1684242/1‑130 (MQ=255) gAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCt > 4:1129088/1‑149 (MQ=255) aCCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTaa < 4:1770929/149‑1 (MQ=255) ccAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTaaa < 2:928794/149‑1 (MQ=255) cAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAg > 4:1769833/1‑149 (MQ=255) aaaGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCAGCGGTTTGACTTTCTTAAAGc > 3:593182/1‑149 (MQ=255) aGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCa < 4:311754/149‑1 (MQ=255) aGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCa < 1:1609313/149‑1 (MQ=255) gC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCa > 1:1343374/1‑148 (MQ=255) gC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCa < 2:1343374/148‑1 (MQ=255) gC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAg < 4:593182/149‑1 (MQ=255) aGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACa > 1:166010/1‑106 (MQ=255) aGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACa < 2:166010/106‑1 (MQ=255) aGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCg > 2:1436225/1‑114 (MQ=255) tGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTaaa > 4:1141608/1‑133 (MQ=255) tGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTaaa < 3:1141608/133‑1 (MQ=255) aGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCt < 2:1336934/149‑1 (MQ=255) | TCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAGGCAGCGCCTTTTCT > NC_000913/2705986‑2706282 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |