Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,888,212 Δ1 bp 10.3% intergenic (+20/‑223) mnmE → / → tnaC tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,888,2120T.10.3% 300.3 / 28.6 87intergenic (+20/‑223)mnmE/tnaCtRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide
Reads supporting (aligned to +/- strand):  ref base T (36/42);  new base . (4/5);  total (40/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

GCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCAC  >  NC_000913/3888064‑3888358
                                                                                                                                                    |                                                                                                                                                   
gCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACTGCGCTTACGAAGCCGCAt                                                                                                                                                     >  1:1738286/1‑149 (MQ=255)
      gCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGAc                                                                                                                                               <  3:387311/149‑1 (MQ=255)
      gCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGAca                                                                                                                                              >  2:1770488/1‑148 (MQ=255)
         ggTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGt                                                                                                                                            <  3:987370/149‑1 (MQ=255)
         ggTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGt                                                                                                                                            <  3:342901/149‑1 (MQ=255)
          gTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc                                                                                                                                           >  3:1700053/1‑149 (MQ=255)
                tgctgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGc                                                                                                                                    <  4:596793/149‑1 (MQ=255)
                tgctgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGc                                                                                                                                    <  3:277466/149‑1 (MQ=255)
                tgctgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGc                                                                                                                                    <  2:592040/149‑1 (MQ=255)
                tgctgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGaatgc                                                                                                                                   <  1:1770488/149‑5 (MQ=255)
                 gctgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGaatgcg                                                                                                                                  <  1:1629292/149‑6 (MQ=255)
                     gCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgctt                                                                                                                               <  4:1700053/149‑1 (MQ=255)
                       ggAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcg                                                                                                                             >  4:1680994/1‑149 (MQ=255)
                        gAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACTGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgc                                                                                                                            <  2:1738286/149‑1 (MQ=255)
                        gAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATg                                                                                                                                     <  2:798060/140‑1 (MQ=255)
                        gAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATg                                                                                                                                     >  1:798060/1‑140 (MQ=255)
                           gagTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                                                                         <  3:1680994/149‑1 (MQ=255)
                              ttGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGAc                                                                                                                                               >  2:1300682/1‑125 (MQ=255)
                              ttGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGAc                                                                                                                                               <  1:1300682/125‑1 (MQ=255)
                              ttGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAtt                                                                                                                      >  2:35320/1‑149 (MQ=255)
                                   tCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgct                                                                                                                                >  3:333654/1‑134 (MQ=255)
                                   tCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgct                                                                                                                                <  4:333654/134‑1 (MQ=255)
                                       gCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACt                                                                                                             >  1:100231/1‑149 (MQ=255)
                                              agAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGt                                                                                                                    >  3:98711/1‑135 (MQ=255)
                                              agAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGt                                                                                                                    <  4:98711/135‑1 (MQ=255)
                                               gAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                                                                 <  3:539527/121‑1 (MQ=39)
                                               gAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                                                                 >  4:539527/1‑121 (MQ=39)
                                                   ttAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc                                                                                                                                           >  1:234308/1‑108 (MQ=255)
                                                   ttAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc                                                                                                                                           <  2:891701/108‑1 (MQ=255)
                                                   ttAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc                                                                                                                                           <  2:234308/108‑1 (MQ=255)
                                                   ttAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc                                                                                                                                           >  1:891701/1‑108 (MQ=255)
                                                       gCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttc                                                                                                                              <  3:1873465/116‑1 (MQ=255)
                                                       gCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttc                                                                                                                              >  4:1873465/1‑116 (MQ=255)
                                                         gAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc                                                                                                                                           <  4:1050906/102‑1 (MQ=255)
                                                         gAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc                                                                                                                                           >  3:1050906/1‑102 (MQ=255)
                                                             tCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt                                                                                       >  2:1125825/1‑149 (MQ=255)
                                                             tCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt                                                                                       >  4:1659889/1‑149 (MQ=255)
                                                              cccgggggATTTTACTTCAGACGACCGGCTGGGGCGGTTTTCCTCCGCCTTCTGAATGGTTAATTACCCCCGTTTACAAAGCCGAATTCTGACGGCCAGATGCGGCTTCGCTTCATTTTACCACTCCTGTTATTCCTCAACCCtttttt                                                                                     <  1:35320/145‑1 (MQ=255)
                                                               aCCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAt                                                                                                                       <  4:1630417/115‑1 (MQ=255)
                                                               aCCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAt                                                                                                                       >  3:1630417/1‑115 (MQ=255)
                                                               aCCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttttt                                                                                     <  2:100231/149‑1 (MQ=255)
                                                                      aaTTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCa                                                                                                               <  3:943854/116‑1 (MQ=255)
                                                                      aaTTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCa                                                                                                               >  4:943854/1‑116 (MQ=255)
                                                                       aTTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttt                                                                                        <  3:119870/138‑1 (MQ=255)
                                                                       aTTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttt                                                                                        >  4:119870/1‑138 (MQ=255)
                                                                        tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTa                                                                            <  1:1125825/149‑1 (MQ=255)
                                                                        tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTa                                                                            <  3:1659889/149‑1 (MQ=255)
                                                                             ttCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTcc                                                                                                           >  1:1725353/1‑113 (MQ=255)
                                                                             ttCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTcc                                                                                                           <  2:1725353/113‑1 (MQ=255)
                                                                             ttCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAAtt                                                                       >  3:154689/1‑149 (MQ=255)
                                                                                aGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt                                                                                       <  1:1855875/130‑1 (MQ=255)
                                                                                aGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt                                                                                       >  2:1855875/1‑130 (MQ=255)
                                                                                 gacgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGaatgc                                                                                                                                   <  1:1115350/84‑5 (MQ=38)
                                                                                 gacgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGaatgc                                                                                                                                   >  2:1115350/1‑80 (MQ=38)
                                                                                  acgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttttt                                                                                      <  3:626199/129‑1 (MQ=255)
                                                                                  acgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttttt                                                                                      >  4:626199/1‑129 (MQ=255)
                                                                                       ctgctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                                                                 <  1:9225/97‑1 (MQ=255)
                                                                                       ctgctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                                                                 >  2:9225/1‑97 (MQ=255)
                                                                                               gCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAt                                                                                                     >  1:1323225/1‑101 (MQ=255)
                                                                                               gCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAt                                                                                                     <  2:1323225/101‑1 (MQ=255)
                                                                                                    ttttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCt                                                                                                 <  3:234162/100‑1 (MQ=255)
                                                                                                    ttttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCt                                                                                                 >  4:234162/1‑100 (MQ=255)
                                                                                                    ttttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                                                                 >  1:366626/1‑116 (MQ=255)
                                                                                                    ttttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                                                                 <  2:366626/116‑1 (MQ=255)
                                                                                                    ttttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTa                                                                   <  2:1401621/130‑1 (MQ=255)
                                                                                                    ttttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTa                                                                   >  1:1401621/1‑130 (MQ=255)
                                                                                                           cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAt                                                                                                                       >  4:520362/1‑71 (MQ=255)
                                                                                                           cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAt                                                                                                                       <  3:520362/71‑1 (MQ=255)
                                                                                                           cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTg                                                                                                         >  3:1469358/1‑85 (MQ=255)
                                                                                                           cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTg                                                                                                         <  4:1469358/85‑1 (MQ=255)
                                                                                                                  tGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                                                                 >  1:1182447/1‑102 (MQ=255)
                                                                                                                  tGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                                                                 <  2:1182447/102‑1 (MQ=255)
                                                                                                                        ggTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCt                            <  4:154689/149‑1 (MQ=255)
                                                                                                                           aagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAt                                                                                                                       <  2:1343931/55‑1 (MQ=255)
                                                                                                                           aagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAt                                                                                                                       >  1:1343931/1‑55 (MQ=255)
                                                                                                                            agtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTc                                                                      <  1:1203629/103‑1 (MQ=255)
                                                                                                                            agtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTc                                                                      >  2:1203629/1‑103 (MQ=255)
                                                                                                                                       ttACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAAt                                                                        >  2:555362/1‑90 (MQ=255)
                                                                                                                                       ttACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAAt                                                                        <  1:555362/90‑1 (MQ=255)
                                                                                                                                           gAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                                                             >  3:845420/1‑97 (MQ=255)
                                                                                                                                           gAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                                                             <  4:845420/97‑1 (MQ=255)
                                                                                                                                             aGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTc                                                                                                            >  2:1630492/1‑48 (MQ=255)
                                                                                                                                             aGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTc                                                                                                            <  1:1630492/48‑1 (MQ=255)
                                                                                                                                               ccGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt                                                                                       <  3:700839/67‑1 (MQ=255)
                                                                                                                                               ccGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt                                                                                       >  4:700839/1‑67 (MQ=255)
                                                                                                                                               ccGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAAc                    <  1:726512/134‑1 (MQ=255)
                                                                                                                                               ccGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAAc                    >  2:726512/1‑134 (MQ=255)
                                                                                                                                                 gCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTca   >  3:337710/1‑149 (MQ=255)
                                                                                                                                                    ttCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTcac  <  2:253350/147‑1 (MQ=255)
                                                                                                                                                    ttCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTcac  >  1:253350/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCAC  >  NC_000913/3888064‑3888358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: