Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 813,570:1 +T 5.1% coding (45/2022 nt) uvrB → exision nuclease of nucleotide excision repair, DNA damage recognition component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913813,5701.T 5.1% 690.9 / 30.2 156coding (45/2022 nt)uvrBexision nuclease of nucleotide excision repair, DNA damage recognition component
Reads supporting (aligned to +/- strand):  ref base . (77/71);  new base T (4/4);  total (81/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 157 total aligned reads displayed.

GGTGATGAACTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGC  >  NC_000913/813425‑813718
                                                                                                                                                  |                                                                                                                                                    
  tgatgaACTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCa                                                                                                                                                 <  4:867645/149‑1 (MQ=255)
    atgaACTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGc                                                                                                                                               >  2:1579430/1‑149 (MQ=255)
         cTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCagag                                                                                                                                          >  4:1378792/1‑149 (MQ=255)
         cTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCagag                                                                                                                                          >  2:1453994/1‑149 (MQ=255)
            tttttttATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCa                                                                                                                                                 <  3:1839928/139‑1 (MQ=255)
            tttttttATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCa                                                                                                                                                 >  4:1839928/1‑139 (MQ=255)
              tttttATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGAt                                                                                                                                     <  1:1453994/149‑1 (MQ=255)
                   aTCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGAc                                                                                                                                <  3:1347860/149‑1 (MQ=255)
                    tCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCTGATCGCCagaaggt                                                                                                                                       <  3:914652/141‑5 (MQ=255)
                    tCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCTGATCGCCagaagg                                                                                                                                        >  3:1031674/1‑137 (MQ=255)
                    tCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCTGATCGCCagaagg                                                                                                                                        <  4:1031674/140‑4 (MQ=255)
                      cAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGCTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGAt                                                                                                                                                   >  2:499530/1‑127 (MQ=255)
                         tataATTTATTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGAtt                                                                                                                                    >  4:272423/1‑139 (MQ=255)
                         tataATTTATTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGAtt                                                                                                                                    <  3:272423/139‑1 (MQ=255)
                           taATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGaa                                                                                                                        <  3:1558408/149‑1 (MQ=255)
                             aTTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAaga                                                                                                                      >  3:990620/1‑149 (MQ=255)
                                     gCATAATTAAGTACGACGAGTAAAGTTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCCAGCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTgg                                                                                                              >  3:45441/1‑149 (MQ=255)
                                     gCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCTGATCGCCagaagg                                                                                                                                        >  2:1663872/1‑120 (MQ=255)
                                     gCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCTGATCGCCagaagg                                                                                                                                        <  1:1663872/123‑4 (MQ=255)
                                     gCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTgg                                                                                                              >  4:380752/1‑149 (MQ=255)
                                       aTAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAgg                                                                                                                    <  3:722680/141‑1 (MQ=255)
                                       aTAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAgg                                                                                                                    <  1:910527/141‑1 (MQ=255)
                                       aTAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAgg                                                                                                                    >  4:722680/1‑141 (MQ=255)
                                          aTTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGaa                                                                                                                        >  4:1418984/1‑134 (MQ=255)
                                          aTTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGaa                                                                                                                        <  3:1418984/134‑1 (MQ=255)
                                          aTTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGAt                                                                                                         <  3:380752/149‑1 (MQ=255)
                                           ttAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGtct                                                                                                                            >  3:169667/1‑129 (MQ=255)
                                           ttAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGtct                                                                                                                            <  4:169667/129‑1 (MQ=255)
                                           ttAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGtct                                                                                                                            <  4:857228/129‑1 (MQ=255)
                                                       aGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGc                                                                                                                                        >  3:622636/1‑105 (MQ=255)
                                                       aGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGc                                                                                                                                        <  4:622636/105‑1 (MQ=255)
                                                        gTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCaga                                                                                                                                           >  1:586435/1‑101 (MQ=255)
                                                        gTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCaga                                                                                                                                           <  2:586435/101‑1 (MQ=255)
                                                         tAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCTGATCGCCagaa                                                                                                                                          <  4:971198/101‑2 (MQ=255)
                                                         tAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAgg                                                                                                                    >  4:688104/1‑123 (MQ=255)
                                                           aaaTTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGAc                                                                                        >  2:1716642/1‑149 (MQ=255)
                                                                      cTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGa                                                                                                                                 >  2:1178826/1‑97 (MQ=255)
                                                                      cTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGa                                                                                                                                 <  1:1178826/97‑1 (MQ=255)
                                                                       tGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGt                                                                            >  1:1640564/1‑149 (MQ=255)
                                                                         cccgcccAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGa                                                                          <  3:1106466/149‑1 (MQ=255)
                                                                              ccAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGt                                                                            >  1:1418263/1‑142 (MQ=255)
                                                                                aaCTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTc                                                                   >  1:744060/1‑149 (MQ=255)
                                                                                aaCTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTc                                                                   >  2:1402242/1‑149 (MQ=255)
                                                                                 aCTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTa                                                                                    <  1:1767680/131‑1 (MQ=255)
                                                                                   tCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAgg                                                                <  4:511075/149‑1 (MQ=255)
                                                                                     cTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGAc                                                                         <  4:947329/138‑1 (MQ=255)
                                                                                      ttCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGaa                                                             >  4:236605/1‑149 (MQ=255)
                                                                                      ttCACGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGAt                                                                                                                                     <  4:1552298/77‑1 (MQ=255)
                                                                                         aGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAg                                                                 >  4:1663939/1‑142 (MQ=255)
                                                                                           gTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGa                                                                                         <  2:1807117/116‑1 (MQ=255)
                                                                                           gTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGa                                                                                         >  1:1807117/1‑116 (MQ=255)
                                                                                           gTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTgg                                                                      <  2:567377/135‑1 (MQ=255)
                                                                                            tAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGt                                                                            <  2:497272/128‑1 (MQ=255)
                                                                                            tAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGt                                                                            >  1:497272/1‑128 (MQ=255)
                                                                                            tAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAgg                                                                >  4:399466/1‑140 (MQ=255)
                                                                                            tAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAgg                                                                <  3:399466/140‑1 (MQ=255)
                                                                                             aGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGc                                                                                                                                               <  3:1634520/60‑1 (MQ=255)
                                                                                             aGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGc                                                                                                                                               >  4:1634520/1‑60 (MQ=255)
                                                                                             aGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTc                                                      <  3:236605/149‑1 (MQ=255)
                                                                                              gCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCt                                                                                                    >  2:98075/1‑102 (MQ=255)
                                                                                               cGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCAc                                                    <  3:1685077/149‑1 (MQ=255)
                                                                                                gACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCAcc                                                   >  1:1729379/1‑149 (MQ=255)
                                                                                                gACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCAcc                                                   >  1:116674/1‑149 (MQ=255)
                                                                                                    cATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACtt                                                                                 <  1:502994/115‑1 (MQ=255)
                                                                                                     aTGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGc                                              >  2:1010494/1‑149 (MQ=255)
                                                                                                     aTGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGAGTGACTGGCTCAGGGAAAACCTTCACCATTGc                                              >  2:1651552/1‑149 (MQ=255)
                                                                                                         gTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAaga                                                                                                                      <  1:1228391/73‑1 (MQ=255)
                                                                                                         gTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGa                                                                          >  3:1850326/1‑117 (MQ=255)
                                                                                                         gTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGa                                                                          <  4:1850326/117‑1 (MQ=255)
                                                                                                          tAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATg                                         >  2:301561/1‑149 (MQ=255)
                                                                                                          tAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATg                                         >  3:1752293/1‑149 (MQ=255)
                                                                                                            aaCCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGt                                                                                      <  3:1443142/102‑1 (MQ=255)
                                                                                                             aCCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGAc                                                                                        <  1:1435194/99‑1 (MQ=255)
                                                                                                             aCCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGAc                                                                                        >  2:1435194/1‑99 (MQ=255)
                                                                                                               cGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCa                                                                  <  3:1136262/119‑1 (MQ=255)
                                                                                                               cGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCa                                                                  >  4:1136262/1‑119 (MQ=255)
                                                                                                                 ttCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACtt                                                                                 <  4:1698054/102‑1 (MQ=255)
                                                                                                                    aaaCTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGa                               <  2:744060/149‑1 (MQ=255)
                                                                                                                     aaCTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTg                                                                                >  2:543810/1‑99 (MQ=255)
                                                                                                                     aaCTGAATTCCGCTTATAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTg                                                                                <  1:543810/99‑1 (MQ=255)
                                                                                                                        tGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGc                                                                                                                 <  1:569101/63‑1 (MQ=255)
                                                                                                                          aaTTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCa                         <  3:692684/149‑1 (MQ=255)
                                                                                                                               cGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGcc                    >  2:1018275/1‑149 (MQ=255)
                                                                                                                                gCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGAc                              <  4:1831782/138‑1 (MQ=255)
                                                                                                                                gCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGccc                   >  3:68373/1‑149 (MQ=255)
                                                                                                                                   tttAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAAc                <  3:952669/149‑1 (MQ=255)
                                                                                                                                      aatccTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCAt             <  2:116674/146‑1 (MQ=255)
                                                                                                                                      aaaCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCAt             <  2:1729379/149‑1 (MQ=255)
                                                                                                                                       aaCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAGACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATg            >  2:1358817/1‑149 (MQ=255)
                                                                                                                                       aaCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCAta            <  1:1010494/149‑2 (MQ=255)
                                                                                                                                       aaCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATg            <  1:1358817/149‑1 (MQ=255)
                                                                                                                                       aaCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATg            <  1:301561/149‑1 (MQ=255)
                                                                                                                                        aCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGa                                                                          <  3:946421/86‑1 (MQ=255)
                                                                                                                                        aCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGa                                                                          >  4:946421/1‑86 (MQ=255)
                                                                                                                                        aCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGAc                              >  1:792064/1‑130 (MQ=255)
                                                                                                                                        aCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGAc                              <  2:792064/130‑1 (MQ=255)
                                                                                                                                        aCCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATgg           <  1:1018275/149‑1 (MQ=255)
                                                                                                                                           ttCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTa         >  4:1319632/1‑148 (MQ=255)
                                                                                                                                           ttCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTAc        >  4:711939/1‑149 (MQ=255)
                                                                                                                                           ttCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTAc        >  2:176777/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGTGATGAACTGTTTTTTTATCCAGTATAATTTGTTGGCATAATTAAGTACGACGAGTAAAATTACATACCTGCCCGCCCAACTCCTTCAGGTAGCGACTCATGAGTAAACCGTTCAAACTGAATTCCGCTTTTAAACCTTCTGGCGATCAGCCAGAGGCGATTCGACGTCTCGAAGAGGGGCTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGC  >  NC_000913/813425‑813718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: