Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,747,168:1 +C 6.9% coding (85/1497 nt) lyxK → L‑xylulose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,747,1681.C 6.9% 1575.4 / 112.2 361coding (85/1497 nt)lyxKL‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base . (169/167);  new base C (12/13);  total (181/180)
Fisher's exact test for biased strand distribution p-value = 8.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01

Only 100 of 380 total aligned reads displayed.

TTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCAT  >  NC_000913/3747021‑3747316
                                                                                                                                                    |                                                                                                                                                    
tttgttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGt                                                                                                                                                     <  1:1822375/149‑1 (MQ=255)
   gttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCa                                                                                                                                                  <  3:728785/149‑1 (MQ=255)
    ttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGt                                                                                                                                                     >  1:698448/1‑145 (MQ=255)
         gATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCa                                                                                                                                                  <  2:909314/143‑1 (MQ=255)
         gATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCa                                                                                                                                                  >  1:1226837/1‑143 (MQ=255)
          aTGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCACGCCTg                                                                                                                                           <  1:1288162/149‑1 (MQ=255)
          aTGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTg                                                                                                                                           >  2:1503586/1‑149 (MQ=255)
               ccGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCt                                                                                                                                      >  2:182795/1‑149 (MQ=255)
                    gggAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTgcg                                                                                                                                 <  1:1503586/149‑1 (MQ=255)
                        gCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCAtt                                                                                                                             >  1:673020/1‑149 (MQ=255)
                          tGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTaa                                                                                                                           <  1:256436/149‑1 (MQ=255)
                           gATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAg                                                                                                                          <  1:1333203/149‑1 (MQ=255)
                             tCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTaa                                                                                                                           >  2:988126/1‑146 (MQ=255)
                               tgctgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCg                                                                                                                      >  1:756129/1‑149 (MQ=255)
                                 ctgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGcc                                                                                                                                         <  2:1694674/128‑1 (MQ=255)
                                      aGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCa                                                                                                                                                  <  1:363083/114‑1 (MQ=255)
                                      aGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCa                                                                                                                                                  >  2:363083/1‑114 (MQ=255)
                                        ccGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAgc                                                                                                                                                <  3:386562/114‑1 (MQ=255)
                                         cGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAg                                                                                                                          >  2:837301/1‑135 (MQ=255)
                                          gTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTg                                                                                                           >  2:1413472/1‑149 (MQ=255)
                                           tGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCtgt                                                                                                                                    <  2:70992/123‑1 (MQ=255)
                                            ggATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTggg                                                                                                         <  2:1434451/149‑1 (MQ=255)
                                             gATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCACGCCTGc                                                                                                                                          >  1:931226/1‑115 (MQ=255)
                                             gATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGc                                                                                                        >  4:727147/1‑149 (MQ=255)
                                               tGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCACGCCTGc                                                                                                                                          <  4:312788/113‑1 (MQ=255)
                                                   gTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAg                                                                                                               <  1:562136/136‑1 (MQ=255)
                                                    tGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcgc                                                                                                 >  2:1739487/1‑149 (MQ=255)
                                                      cAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGa                                                                                               <  3:1593284/149‑1 (MQ=255)
                                                       aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGat                                                                                              >  3:821656/1‑149 (MQ=255)
                                                       aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGat                                                                                              >  2:1615983/1‑149 (MQ=255)
                                                       aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGat                                                                                              >  3:1490500/1‑149 (MQ=255)
                                                          aaaCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATg                                                                                           <  1:1404598/149‑1 (MQ=255)
                                                            aCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTgg                                                                                                          >  2:1290927/1‑132 (MQ=255)
                                                             ccATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCa                                                                                        >  3:1135234/1‑149 (MQ=255)
                                                             ccATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCa                                                                                        >  3:780023/1‑149 (MQ=255)
                                                              cATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCtgtg                                                                                                                                   <  2:1092471/105‑1 (MQ=255)
                                                              cATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCtgtg                                                                                                                                   >  1:1092471/1‑105 (MQ=255)
                                                              cATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCg                                                                                                                      >  4:364529/1‑118 (MQ=255)
                                                                   cGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtg                                                                                  >  2:1009547/1‑149 (MQ=255)
                                                                   cGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtg                                                                                  >  1:1688063/1‑149 (MQ=255)
                                                                    gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                                                                                 <  4:780023/149‑1 (MQ=255)
                                                                     cAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgtg                                                                                <  4:16873/149‑1 (MQ=255)
                                                                      aaTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTgg                                                                               <  1:1009547/149‑1 (MQ=255)
                                                                        tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCa                                                                             >  1:840742/1‑149 (MQ=255)
                                                                          ctggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                                                                                            >  3:565212/1‑132 (MQ=255)
                                                                          ctggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                                                                                            <  4:565212/132‑1 (MQ=255)
                                                                            ggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgc                                                                         <  4:1153107/149‑1 (MQ=255)
                                                                            ggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgc                                                                         <  4:1522375/149‑1 (MQ=255)
                                                                                  ggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt                                                                           >  4:1305627/1‑141 (MQ=255)
                                                                                   gTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATgg                                                                  >  3:938143/1‑149 (MQ=255)
                                                                                   gTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATgg                                                                  <  1:1615983/149‑1 (MQ=255)
                                                                                   gTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATgg                                                                  >  1:398597/1‑149 (MQ=255)
                                                                                    ttAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                                                                                 >  1:349982/1‑133 (MQ=255)
                                                                                        aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTc                                                             >  3:1194753/1‑149 (MQ=255)
                                                                                           gtgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAgg                                                                                                              <  4:1461960/97‑1 (MQ=255)
                                                                                             ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGc                                                                                         <  2:946820/116‑1 (MQ=255)
                                                                                             ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcg                                                        <  1:1739487/149‑1 (MQ=255)
                                                                                             ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcg                                                        <  3:1309817/149‑1 (MQ=255)
                                                                                              gCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcgc                                                       <  2:754981/149‑1 (MQ=255)
                                                                                                   aGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCa                                                                             >  4:255079/1‑122 (MQ=255)
                                                                                                      tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAgc                                                                                                                                                <  4:1861345/52‑1 (MQ=255)
                                                                                                      tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAgc                                                                                                                                                >  3:1861345/1‑52 (MQ=255)
                                                                                                      tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTgg                                                                               >  2:1173736/1‑117 (MQ=255)
                                                                                                      tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTgg                                                                               <  1:1173736/117‑1 (MQ=255)
                                                                                                        gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGc                                                                                                                     >  2:676329/1‑77 (MQ=255)
                                                                                                          tgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGa                                                                                               <  2:510147/97‑1 (MQ=255)
                                                                                                               gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCgcgc                                                     <  4:891513/134‑1 (MQ=255)
                                                                                                                  gggCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTgg                                   <  2:398597/149‑1 (MQ=255)
                                                                                                                   ggCTGTATGACTGCGAAGGCCGGGAGGCAGTCGTGCAGCGCCTGCCGCTGTGCGCACTAAGCCCGCAGCCAGGCTGGGCCGAGCGCGATATGGCAGAACAGTGGCAACGCTGCATGGCTGTCATTCGCGCCATGCTTACTCATTCTGgt                                  <  4:1171709/149‑1 (MQ=255)
                                                                                                                   ggCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgt                                  >  3:324784/1‑149 (MQ=255)
                                                                                                                   ggCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgt                                  <  1:1864681/149‑1 (MQ=255)
                                                                                                                   ggCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgt                                  >  3:1359824/1‑149 (MQ=255)
                                                                                                                     cTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCGTTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgtgt                                >  4:1238539/1‑149 (MQ=255)
                                                                                                                     cTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCAGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgtgt                                <  4:1583783/149‑1 (MQ=255)
                                                                                                                        tATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTc                                                         <  1:1423547/121‑1 (MQ=255)
                                                                                                                        tATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAg                             >  2:1057370/1‑149 (MQ=255)
                                                                                                                           gACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCgg                          <  4:1359824/149‑1 (MQ=255)
                                                                                                                            actgcgAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCggg                         >  1:1034065/4‑149 (MQ=255)
                                                                                                                            aCCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCggg                         <  2:354420/149‑1 (MQ=255)
                                                                                                                              cgcgAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGc                                                                                                        >  2:602282/1‑68 (MQ=255)
                                                                                                                              cgcgAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgt                                  >  4:10585/1‑138 (MQ=255)
                                                                                                                               gcgAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTgg                                                                               >  2:1364203/1‑92 (MQ=255)
                                                                                                                               gcgAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGaa                      >  3:271862/1‑149 (MQ=255)
                                                                                                                                 gAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTAc                                            <  3:63408/125‑1 (MQ=255)
                                                                                                                                 gAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTAc                                            >  4:63408/1‑125 (MQ=255)
                                                                                                                                       nnnnaggcaggcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGt              >  2:1221939/5‑149 (MQ=255)
                                                                                                                                       cGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGt              <  3:1564375/149‑1 (MQ=255)
                                                                                                                                             gcaggcGCTGCACGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgtg                                                                                <  3:880996/77‑1 (MQ=255)
                                                                                                                                             gcaggcGCTGCACGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTAt        >  2:248394/1‑149 (MQ=255)
                                                                                                                                             gcaggcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTAt        <  2:192667/149‑1 (MQ=255)
                                                                                                                                               aggcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTgg                                                                               >  3:1255157/1‑76 (MQ=255)
                                                                                                                                               aggcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgtg                                 <  4:846913/122‑1 (MQ=255)
                                                                                                                                               aggcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTa                              <  1:470484/125‑1 (MQ=255)
                                                                                                                                               aggcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCg      >  3:1700692/1‑149 (MQ=255)
                                                                                                                                               aggcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCg      >  3:470835/1‑149 (MQ=255)
                                                                                                                                               aggcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCg      >  1:890321/1‑149 (MQ=255)
                                                                                                                                                 gcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGtt                               <  3:1156723/122‑1 (MQ=255)
                                                                                                                                                 gcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGc    >  1:958157/1‑149 (MQ=255)
                                                                                                                                                  cGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTc             <  1:732209/139‑1 (MQ=255)
                                                                                                                                                   gTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCAt  <  4:470835/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCAT  >  NC_000913/3747021‑3747316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: