Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,000,238 T→A 7.8% intergenic (+93/+54) uvrD → / ← yigE DNA‑dependent ATPase I and helicase II/DUF2233 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,000,2380TA 7.8% 539.9 / 27.9 154intergenic (+93/+54)uvrD/yigEDNA‑dependent ATPase I and helicase II/DUF2233 family protein
Reads supporting (aligned to +/- strand):  ref base T (73/69);  new base A (6/6);  total (79/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.81e-01

Only 100 of 155 total aligned reads displayed.

GCCAGGGTATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCC  >  NC_000913/4000093‑4000384
                                                                                                                                                 |                                                                                                                                                  
gCCAGGGTATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTAcg                                                                                                                                                 <  2:1210905/149‑1 (MQ=255)
       tATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATc                                                                                                                                          <  3:303646/149‑1 (MQ=255)
        aTTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATGCGGCGTAAACGCCCTATcc                                                                                                                                         <  3:564848/149‑1 (MQ=255)
               ggCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATGCGGCGTAAAcgccctat                                                                                                                                           >  3:679104/1‑136 (MQ=255)
                 cTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTgc                                                                                                                                <  4:1561642/149‑1 (MQ=255)
                  tggtggCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTgcg                                                                                                                               <  2:453812/149‑1 (MQ=255)
                  tggtggCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTgcg                                                                                                                               >  2:851992/1‑149 (MQ=255)
                         ggcATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCGTCCGGCATCTGCGCCATCtt                                                                                                                        <  2:1469926/149‑1 (MQ=255)
                                gCCCGGCTGGAGTCGGTGTAACGTTGCCTGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATc                                                                                                                 >  1:614137/1‑149 (MQ=255)
                                  ccGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTg                                                                                                               >  3:1064504/1‑149 (MQ=255)
                                     gCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCa                                                                                                                      <  2:1436266/139‑1 (MQ=255)
                                               gtgtAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCt                                                                                                                >  4:321946/1‑135 (MQ=255)
                                                  tAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATc                                                                                                                 >  3:1794520/1‑131 (MQ=255)
                                                  tAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATc                                                                                                                 >  3:1074798/1‑131 (MQ=255)
                                                  tAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATc                                                                                                                 <  4:1074798/131‑1 (MQ=255)
                                                  tAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATc                                                                                                                 <  4:1794520/131‑1 (MQ=255)
                                                             gATGCGGTTCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTcc                                                                                    >  4:159831/1‑149 (MQ=255)
                                                                        cagcACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGaa                                                                         <  2:1048594/147‑1 (MQ=255)
                                                                           cACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATc                                                                      >  2:921596/1‑149 (MQ=255)
                                                                             gcTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACaaaa                                                                                                       <  4:1521387/113‑1 (MQ=255)
                                                                             gcTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACaaaa                                                                                                       >  3:1521387/2‑114 (MQ=255)
                                                                             ccTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCTGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACaaaa                                                                                                       >  1:1680484/1‑114 (MQ=255)
                                                                             ccTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACaaaa                                                                                                       <  2:1680484/114‑1 (MQ=255)
                                                                             ccTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCAt                                                                    >  4:863075/1‑149 (MQ=255)
                                                                              cTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCt                                                                                        >  4:969578/1‑128 (MQ=255)
                                                                              cTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCt                                                                                        <  3:969578/128‑1 (MQ=255)
                                                                              cTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATg                                                                   <  3:159831/149‑1 (MQ=255)
                                                                              cTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATg                                                                   >  2:519463/1‑149 (MQ=255)
                                                                              cTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCAGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGtt                                                                                               <  1:1218310/121‑1 (MQ=255)
                                                                                 aTTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGt                                                                 <  3:865787/148‑1 (MQ=255)
                                                                                         tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATGCGGCGTAAAcgcc                                                                                                                                               >  3:834691/1‑62 (MQ=25)
                                                                                         tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATGCGGCGTAAAcgcc                                                                                                                                               <  1:1609470/62‑1 (MQ=25)
                                                                                         tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATGCGGCGTAAAcgcc                                                                                                                                               <  4:834691/62‑1 (MQ=25)
                                                                                         tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGt                                                                                                <  3:488508/109‑1 (MQ=255)
                                                                                         tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGt                                                                                                >  4:488508/1‑109 (MQ=255)
                                                                                         tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTaa                                                                                           <  4:11961/114‑1 (MQ=255)
                                                                                         tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAgg                                                        >  3:1366127/1‑149 (MQ=255)
                                                                                            aaaaTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTc                                                                                     <  2:1275655/117‑1 (MQ=255)
                                                                                              aaTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGc                                                   >  3:1166689/1‑149 (MQ=255)
                                                                                               aTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGt                                                                 <  3:242496/134‑1 (MQ=255)
                                                                                               aTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGt                                                                 >  4:242496/1‑134 (MQ=255)
                                                                                                   ttCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATc                                                                                                  <  4:535357/97‑1 (MQ=255)
                                                                                                    tCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATa                                                     <  2:1171208/141‑1 (MQ=255)
                                                                                                    tCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGcc                                             >  1:1131877/1‑149 (MQ=255)
                                                                                                      aGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAAc                                                                                          <  1:145754/102‑1 (MQ=255)
                                                                                                      aGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGa                                                       >  2:1414651/1‑137 (MQ=255)
                                                                                                      aGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGa                                                       <  1:1414651/137‑1 (MQ=255)
                                                                                                       gATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACg                                          >  3:1098455/1‑149 (MQ=255)
                                                                                                        aTTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACgg                                         <  2:1131877/149‑1 (MQ=255)
                                                                                                         ttCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACaaaa                                                                                                       <  3:1855534/86‑1 (MQ=255)
                                                                                                         ttCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACaaaa                                                                                                       >  4:1855534/1‑86 (MQ=255)
                                                                                                          tCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGtt                                                <  4:895586/140‑1 (MQ=255)
                                                                                                            aaTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATc                                                                      >  4:1144135/1‑116 (MQ=255)
                                                                                                            aaTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACaaaa                                                          <  4:217630/128‑1 (MQ=255)
                                                                                                            aaTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACaaaa                                                          <  4:1069691/128‑1 (MQ=255)
                                                                                                            aaTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACaaaa                                                          >  3:217630/1‑128 (MQ=255)
                                                                                                            aaTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGAtt                                     >  4:1206458/1‑149 (MQ=255)
                                                                                                            aaTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGAtt                                     >  1:1823020/1‑149 (MQ=255)
                                                                                                              tAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGc                                   >  3:1623159/1‑149 (MQ=255)
                                                                                                              tAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGc                                   >  4:913408/1‑149 (MQ=255)
                                                                                                                 aTTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGtt                                                >  2:327189/1‑133 (MQ=255)
                                                                                                                 aTTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGtt                                                <  1:327189/133‑1 (MQ=255)
                                                                                                                     cAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCt                            >  3:425257/1‑149 (MQ=255)
                                                                                                                      aaCGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGAc                                                                             >  4:245697/1‑99 (MQ=255)
                                                                                                                      aaCGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACaaaa                                                          <  2:1825687/118‑1 (MQ=255)
                                                                                                                      aaCGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCtt                           >  2:1234233/1‑149 (MQ=255)
                                                                                                                       aCGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGAtt                                     >  3:315491/1‑138 (MQ=255)
                                                                                                                         gTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCa                        >  4:1724152/1‑149 (MQ=255)
                                                                                                                          tCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTc                                                                                >  4:1144548/1‑92 (MQ=255)
                                                                                                                          tCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTc                                                                                <  3:1144548/92‑1 (MQ=255)
                                                                                                                          tCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCat                       >  1:72987/1‑149 (MQ=255)
                                                                                                                           cATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCata                      <  1:921596/149‑1 (MQ=255)
                                                                                                                           cATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCata                      <  4:1366127/149‑1 (MQ=255)
                                                                                                                             tGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCAtata                    <  3:98324/149‑1 (MQ=255)
                                                                                                                             tGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCAtata                    <  3:1724152/149‑1 (MQ=255)
                                                                                                                               tAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACa                  <  4:1623159/149‑1 (MQ=255)
                                                                                                                               tAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACa                  >  3:1282738/1‑149 (MQ=255)
                                                                                                                                aGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTa                                                                                                    >  2:1663929/1‑66 (MQ=255)
                                                                                                                                  gCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATa                                                     <  1:1421034/111‑1 (MQ=38)
                                                                                                                                  gCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCatat                     >  1:1529216/1‑143 (MQ=255)
                                                                                                                                  gCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCatat                     >  1:1088020/1‑143 (MQ=255)
                                                                                                                                  gCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCatat                     <  2:1529216/143‑1 (MQ=255)
                                                                                                                                   ccGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATAtg              >  2:1402669/1‑149 (MQ=255)
                                                                                                                                    cGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATAtgt             <  1:519463/149‑1 (MQ=255)
                                                                                                                                      gATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATa                                                     <  1:1017225/107‑1 (MQ=255)
                                                                                                                                      gATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATa                                                     >  2:1017225/1‑107 (MQ=255)
                                                                                                                                         aGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAAt        >  2:944826/1‑149 (MQ=255)
                                                                                                                                          gggCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATAtgt             <  2:1111063/143‑1 (MQ=255)
                                                                                                                                          gggCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATc       <  1:743016/149‑1 (MQ=255)
                                                                                                                                           ggCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACgg                                         <  3:722908/114‑1 (MQ=255)
                                                                                                                                           ggCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACgg                                         >  4:722908/1‑114 (MQ=255)
                                                                                                                                           ggCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCata                      <  1:1156018/133‑1 (MQ=255)
                                                                                                                                            gCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCAc                                           >  3:773185/1‑111 (MQ=255)
                                                                                                                                            gCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCAc                                           <  4:773185/111‑1 (MQ=255)
                                                                                                                                            gCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGt     >  3:1454358/1‑149 (MQ=255)
                                                                                                                                            gCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGt     >  1:218861/1‑149 (MQ=255)
                                                                                                                                              gTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTc                         <  3:129234/127‑1 (MQ=255)
                                                                                                                                              gTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGc   <  3:913408/149‑1 (MQ=255)
                                                                                                                                              gTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGc   <  2:658775/149‑1 (MQ=255)
                                                                                                                                               tttACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAACGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGcc  <  1:1234233/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCCAGGGTATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCC  >  NC_000913/4000093‑4000384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: